Scale Up Isolation Of Aaptamine For In Vivo Evaluation Indicates Its Neurobiological Activity Is Linked To The Delta Opioid Receptor, 2019 Dominican University of California
Scale Up Isolation Of Aaptamine For In Vivo Evaluation Indicates Its Neurobiological Activity Is Linked To The Delta Opioid Receptor, Nicole L. Mcintosh, Eptisam Lambo, Laura Millan-Lobo, Fei Li, Li He, Phillip Crews, Jennifer L. Whistler, Tyler Johnson
Opioid receptors belong to the large superfamily of seven transmembrane-spanning (7TM) G protein-coupled receptors (GPCRs). As a class, GPCRs are of fundamental physiological importance mediating the actions of the majority of known neurotransmitters and hormones. The Mu, Delta, and Kappa (MOP, DOP, KOP) opioid receptors are particularly intriguing members of this receptor family as they are the targets involved in many neurobiological diseases such as addiction, pain, stress, anxiety, and depression. Recently we discovered that the aaptamine class of marine sponge derived natural products exhibit selective agonist activity in vitro for the DOP versus MOP receptor. Our findings may explain ...
Synthesis Of A Sulfur Variant For Treatment Of Trypanosomiasis, 2019 University of Nebraska at Omaha
Synthesis Of A Sulfur Variant For Treatment Of Trypanosomiasis, Carlos Vera-Esquivel
Student Research and Creative Activity Fair
Previous work in our lab has found diphenyl ether benzylamines showed a successful response with a micromolar concentration of our lead compound to treat the deadly Trypanosamiosis rhodesience. Furthermore, mammalian cell lines saw promising resistance towards damages. The goal of this study was to synthesize a diphenyl thio benzylamine variant. This variant was more active toward T. b. rhodesience but showed more toxicity to both rat 10 (IC50 mM) and human cell lines (HFF, HC-04, U-2 OS, and HEK293). The selectivity index (ratio of toxicity to activity in the same concentration units (SI)) varied from 55 ...
Seasonal Origins Of Soil Water Used By Trees, 2019 ETH Zurich
Seasonal Origins Of Soil Water Used By Trees, Scott T. Allen, James W. Kirchner, Sabine Braun, Rolf T. W. Siegwolf, Gregory R. Goldsmith
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Rain recharges soil water storages and either percolates downward into aquifers and streams or is returned to the atmosphere through evapotranspiration. Although it is commonly assumed that summer rainfall recharges plant-available water during the growing season, the seasonal origins of water used by plants have not been systematically explored. We characterize the seasonal origins of waters in soils and trees by comparing their midsummer isotopic signatures (δ2H) to seasonal isotopic cycles in precipitation, using a new seasonal origin index. Across 182 Swiss forest sites, xylem water isotopic signatures show that summer rain was not the predominant water source for ...
Bisthioether Stapled Peptides Targeting Polycomb Repressive Complex 2 Gene Repression, 2019 The Graduate Center, City University of New York
Bisthioether Stapled Peptides Targeting Polycomb Repressive Complex 2 Gene Repression, Gan Zhang
All Dissertations, Theses, and Capstone Projects
Interactions between proteins play a key role in nearly all cellular process, and therefore, disruption of such interactions may lead to many different types of cellular dysfunctions. Hence, pathologic protein-protein interactions (PPIs) constitute highly attractive drug targets and hold great potential for developing novel therapeutic agents for the treatment of incurable human diseases. Unfortunately, the identification of PPI inhibitors is an extremely challenging task, since traditionally used small molecule ligands are mostly unable to cover and anchor on the extensive flat surfaces that define those binary protein complexes. In contrast, large biomolecules such as proteins or peptides are ideal fits ...
Refinement Of Production Grade Biodiesel, 2019 GCSU
Refinement Of Production Grade Biodiesel, Campbell Axt, Robert B. Hughley Jr., Sydney Ninneman, Jillian Turner
The modified Burton method for the thermal hydrogen-cracking of peanut oil has been investigated in McGill Research Group since 2009. The successful and reliable production of biodiesel has been achieved since 2014. A hydrocarbon with a viscosity similar to Production Grade Diesel will work in modern diesel engines. The current product has a viscosity significantly lower than production grade diesel. The starting material has a viscosity significantly higher than Production Grade Diesel. Current research is investigating methodologies to mix starting material and product to achieve target viscosity.
Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, 2019 Iowa State University
Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell, Jacek A. Koziel
Agricultural and Biosystems Engineering Publications
The Midwest wine industry has shown a marked increase in growers, hectares planted, wineries, and wine production. This growth coincides with the release of cold-hardy cultivars such as Brianna and Frontenac gris, in 2001 and 2003, respectively. These white grape varieties account for one-third of the total area grown in the state of Iowa. It is generally accepted that the wine aroma profile plays a crucial role in developing a local, sustainable brand. However, the identity of Brianna/Frontenac Gris-based wine aromas and their link to the grape berry chemistry at harvest is unknown. This study aims to preliminarily characterize ...
Oxidative Degradation Of Lignin And Investigation Of Utilization Of Lignin-Derived Materials As Building Blocks For Epoxy Resins, Zhen Fang
Theses and Dissertations--Chemistry
Lignin, the second most abundant biopolymer on earth, is potentially a replaceable source for bulky fuels and chemical feedstocks. There have been numerous reports on methods for the oxidative cleavage of β-O-4 linkages but relatively few reports of how those methods affect other linkages that are present in lignin. We investigated how the β-1 and β-5 linkages respond under oxidative conditions proposed for lignin deconstruction based on their effect on β-O-4 linkages. Mechanochemical treatment of lignin can greatly improve the yield of monomer products and we applied a mechanochemical approach, using powerful ring-and-puck milling to promote lignin degradation. Along with ...
Functionality Of Membrane Proteins Overexpressed And Purified From E. Coli Is Highly Dependent Upon The Strain, 2019 Old Dominion University
Functionality Of Membrane Proteins Overexpressed And Purified From E. Coli Is Highly Dependent Upon The Strain, Khadija Mathieu, Waqas Javed, Sylvain Vallet, Christian Lesterlin, Marie-Pierre Candusso, Feng Ding, Xiaohong Nancy Xu, Christine Ebel, Jean-Michel Jault, Cédric Orelle
Chemistry & Biochemistry Faculty Publications
Overexpression of correctly folded membrane proteins is a fundamental prerequisite for functional and structural studies. One of the most commonly used expression systems for the production of membrane proteins is Escherichia coli. While misfolded proteins typically aggregate and form inclusions bodies, membrane proteins that are addressed to the membrane and extractable by detergents are generally assumed to be properly folded. Accordingly, GFP fusion strategy is often used as a fluorescent proxy to monitor their expression and folding quality. Here we investigated the functionality of two different multidrug ABC transporters, the homodimer BmrA from Bacillus subtilis and the heterodimer PatA/PatB ...
Biogenic Volatiles Emitted From Four Cold-Hardy Grape Cultivars During Ripening, 2019 Iowa State University
Biogenic Volatiles Emitted From Four Cold-Hardy Grape Cultivars During Ripening, Somchai Rice, Devin L. Maurer, Anne Fennell, Murlidhar Dharmadhikari, Jacek A. Koziel
Agricultural and Biosystems Engineering Publications
In this research dataset, we summarize for the first time volatile organic compounds (VOCs) emitted in vivo from ripening wine grapes. We studied four cold-hardy cultivars grown in the Midwestern U.S.: St. Croix, Frontenac, Marquette, and La Crescent. These cultivars have gained popularity among local growers and winemakers, but still very little is known about their performance compared with long-established V. viniferagrapes. Volatiles were collected using two novel approaches: biogenic emissions from grape clusters on a vine and single grape berries. A third approach was headspace collection of volatiles from crushed grapes. Solid-phase microextraction (SPME) was used to collect ...
Syntheses Of A Tin (Iv) Meso-Tetra(4-Pyridyl) Porphyrin Dichloride-Tetrachlorobis (Bipy)2 Ruthenium (Ii) Complex And Studies Of Photophysical Properties Of 1-Nitropyrene, 2018 Stephen F. Austin State University
Syntheses Of A Tin (Iv) Meso-Tetra(4-Pyridyl) Porphyrin Dichloride-Tetrachlorobis (Bipy)2 Ruthenium (Ii) Complex And Studies Of Photophysical Properties Of 1-Nitropyrene, Phillip Sharp
Electronic Theses and Dissertations
Development of a photosensitizer that can work in both aerobic and anaerobic environments would increase the robustness of the cancer treatment known as photodynamic therapy. The development of a photosensitizer was first attempted by synthesizing a tin (IV) meso-tetra (4-pyridyl) porphyrin dichloride-tetrachlorobis (Bipy)2 ruthenium (II) complex. The synthesize of the tin porphyrin was done by a method from literature and had a percent error of 31.58 and 37.31 when comparing the theoretical percentages of carbon (55.39) and nitrogen (12.92) meaning that photosensitizer was not synthesized.
The second type of chromophore that was studied was 1-nitropyrene ...
Photo-Isomerizable Complementary Hydrogen Bond Arrays, 2018 The University of Western Ontario
Photo-Isomerizable Complementary Hydrogen Bond Arrays, Jeffrey S. Pleizier
Electronic Thesis and Dissertation Repository
The use of light as an external stimulus provides a non-invasive technique to induce large changes in physical and chemical properties of a substrate. The incorporation of a photochromic subunit into supramolecular polymeric systems therefore adds a potential "smart" quality for polymer manipulation. The azo moiety (-N=N-) is one of the most popular photochromic units as the trans to cis isomerization results in a large degree of spatial rearrangement. This present thesis exploits the azo group by its incorporation into complementary hydrogen bonded complexes. Hydrogen bonding acceptor groups attached to the periphery of the central azo photoswitch provided strong ...
Inequality, Rubber, And Thermodynamics In Indonesia, 2018 Cleveland State University
Inequality, Rubber, And Thermodynamics In Indonesia, Ernest M. Oleksy
The Downtown Review
Intersectionality has led the charge in ensuring that workplace justice is assured to all people, irrespective of their identities. While intersectionality is a useful theory for explaining inequality, what must not be understated is the contributions that postcolonialism and the blue-collar working identity can have on harsh working conditions. Particularly, miners in Indonesia have had to work in very hostile environments where they are at-risk for sulfur poisoning as they mine for materials to vulcanize rubber. This article serves two purposes. The first is to call attention to how place can help explain the differential experiences of miners in the ...
Copper Grafted Titanium Dioxide In Hydrogels For Photocatalysts, 2018 Atlanta University Center
Copper Grafted Titanium Dioxide In Hydrogels For Photocatalysts, Salem Amsaaed
Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University
This study are based on the premise that the incorporation of metal ions into nano titania-PHEMA [poly (2-hydroxyethyl methacrylate)] hydrogels would enhance the desirable properties in the photodecomposition of pollutants. The investigation are centered in the use of Cu(II) as metal ion of interest. The development of TiO2-PHEMA-Cu hydrogels was conducted, and the characterization of the materials by FT-IR, XRD and fluorescence was performed. The absorption of copper(II) from the solution was monitored by UV-Vis. The FT-IR are found too, be the most effective tool too, analyze the interaction of Cu(II) with PHEMA in the ...
Ray Et Al.: Pheromone Of The Velvet Longhorned Beetle Trichoferus Campestris, 2018 Xavier University
Ray Et Al.: Pheromone Of The Velvet Longhorned Beetle Trichoferus Campestris, Ann M. Ray, Joseph A. Francese, Yunfan Zou, Kristopher Watson, Damon J. Crook, Jocelyn G. Millar
No abstract provided.
Extraction And Purification Of Biologically Active Metabolites From Rhodococcus Sp. Mtm3w5.2, 2018 East Tennessee State University
Extraction And Purification Of Biologically Active Metabolites From Rhodococcus Sp. Mtm3w5.2, Mohrah Alenazi
Electronic Theses and Dissertations
Rhodococcushas been recognized as a potential antibiotic producer. Recently, a strain of Rhodococcussp. MTM3W5.2 was isolated from a soil sample collected in Morristown, Tennessee and was found to produce an inhibitory compound which is active against other related species. The purpose of this research is to extract, purify and analyze the active metabolite. The compound was extracted from RM broth cultures and purified by preliminary fractionation of crude extract through a Sephadex LH-20 column. Further purification was completed using semi-preparative reversed phase column chromatography. Final purification was obtained using multiple rounds of an analytical C18 HPLC column ...
Analysis Of Sterol Diagenesis Stages In A Peat Core From Kamchatka, Russia, 2018 SUNY College of Environmental Science and Forestry
Analysis Of Sterol Diagenesis Stages In A Peat Core From Kamchatka, Russia, Tia Ogus
Peatlands are a critical part of the global carbon cycle. While many studies of peat carbon focus on accumulation, less is known about peat decay. We use the diagenesis of sterols to track the decomposition of peat in a Holocene-length core of a sedge fen on the Kamchatka Peninsula, Russia. Diagenesis can transform sterols as soon as the peat is initially deposited, but they can also remain unchanged for millennia. We have quantified the transformation of sterols into their diagenetic products to measure the maturity of peat and estimate carbon loss. Further, there are two pathways in sterol diagenesis, oxidative ...
Shorter And Improved Access To The Key Tetracyclic Core Of Sarpagine-Macroline-Ajmaline Indole Alkaloids: The Total Synthesis Of Alkaloids Macrocarpines A-G, Talcarpine, N(4)-Methyl-N(4),21-Secotalpinine, Deoxyperaksine, Dihydroperaksine, Talpinine, O-Acetyltalpinine, And N(4)-Methyltalpinine, 2018 University of Wisconsin-Milwaukee
Shorter And Improved Access To The Key Tetracyclic Core Of Sarpagine-Macroline-Ajmaline Indole Alkaloids: The Total Synthesis Of Alkaloids Macrocarpines A-G, Talcarpine, N(4)-Methyl-N(4),21-Secotalpinine, Deoxyperaksine, Dihydroperaksine, Talpinine, O-Acetyltalpinine, And N(4)-Methyltalpinine, Md Toufiqur Rahman
Theses and Dissertations
Extension of the asymmetric Pictet-Spengler (P-S) reaction to bulkier Nb-alkylated tryptophan derivatives resulted in a shorter and improved stereospecific access to the key bicyclo[3.3.1]nonane framework of bioactive C-19 methyl substituted sarpagine/macro-line/ajmaline indole alkaloids with excellent diastereoselectivity via internal asymmetric induction. The asymmetric Pictet-Spengler/Dieckmann protocol with bulky Nb-alkyl substituted systems enabled a more direct and two-step shorter route to this key architecture. Complete stereocontrol of the C-19 methyl function in either the α- or β-configuration was achieved which would enable one to gain rapid access to the crucial intermediates for the total synthesis of ...
Synthesis Of Dicamba Glucosides For The Study Of Environmental Dicamba Drift Effects On Soybeans, 2018 University of Arkansas, Fayetteville
Synthesis Of Dicamba Glucosides For The Study Of Environmental Dicamba Drift Effects On Soybeans, Holly Wallace
Theses and Dissertations
The most popular herbicide used for weed control has been glyphosate for many years in the Midwestern United States. Plants have begun to develop a resistance to glyphosate due to over use of the herbicide. This herbicide resistance has pushed farmers to turn to alternative herbicides such as dicamba and 2,4-D. Recently agrochemical companies have developed genetically modified crops that are resistant to herbicides such as dicamba. These modified crops allow farmers to spray their fields with dicamba without fear of crop damage. Farmers of non-genetically modified crops, however, suffer damage and loss of yield from herbicide drift effects ...
Modulating Patterns Of Two-Phase Flow With Electric Fields, 2018 University of Washington - Seattle Campus
Modulating Patterns Of Two-Phase Flow With Electric Fields, Dingsheng Liu, Bejan Hakimi, Michael Volny, Joelle Rolfs, Robbyn Anand, Rantisek Turecek, Daniel T. Chiu
This paper describes the use of electro-hydrodynamic actuation to control the transition between three major flow patterns of an aqueous-oil Newtonian flowin a microchannel: droplets, beads-on-a-string (BOAS), and multi-stream laminar flow. We observed interesting transitional flow patterns between droplets and BOAS as the electric field was modulated. The ability to control flow patterns of a two-phase fluid in a microchannel adds to the microfluidic tool box and improves our understanding of this interesting fluid behavior.
Spatial Mapping And Profiling Of Metabolite Distributions During Germination, 2018 Iowa State University and Ames Laboratory
Spatial Mapping And Profiling Of Metabolite Distributions During Germination, Adam D. Feenstra, Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna D. Yandeau-Nelson, Basil J. Nikolau, Young-Jin Lee
Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also ...