Probing The Bifunctional Catalytic Activity Of Ceria Nanorods Towards The Cyanosilylation Reaction, 2013 University of Nebraska-Lincoln
Probing The Bifunctional Catalytic Activity Of Ceria Nanorods Towards The Cyanosilylation Reaction, Gonghua Wang, Lu Wang, Xiang Fei, Yunyun Zhou, Renat F. Sabirianov, Wai Ning Mei, Chin Li Cheung
Faculty Publications -- Chemistry Department
Ceria nanorods were demonstrated to be an active, bifunctional catalyst for the cyanosilylation of aldehydes. The catalytic activity of ceria was shown to be positively correlated with a decrease in the coordination numbers of neighbouring oxygen atoms around cerium atoms in the catalyst. Chemisorption and density functional theory studies suggested that the coordinatively unsaturated cerium sites exposed by the surface oxygen vacancy defects functioned as Lewis acid sites and the neighbouring oxygen atoms behaved as Lewis base sites in the catalytic cycle.
Sol-Gel Characteristics For Corrosion Resistance Of Anodised Aluminium, 2013 Technological University Dublin
Sol-Gel Characteristics For Corrosion Resistance Of Anodised Aluminium, Michael Whelan, John Cassidy, Brendan Duffy
Conventional anodising electrolytes based on sulphuric acid, oxalic acid and phosphoric acid have been used to form nanoporous layers on AA3003-H14 and sealed with silane based sol-gel sealers. It is shown that the sol-gel chemistries have varying levels of pore penetration depending on the synthesis conditions. The extent of sol-gel penetration and pore sealing is analysed by electron microscopy and energy dispersive x-ray spectroscopy. To describe the sealing phenomena observed a sol-gel penetration and sealing rating has been proposed to explain the interactions of the sol-gels with the pores of the anodised layers. The corrosion resistance of the sol-gel sealed ...
A Supramolecular Strategy To Assemble Multifunctional Viral Nanoparticles, 2013 University of South Carolina - Columbia
A Supramolecular Strategy To Assemble Multifunctional Viral Nanoparticles, L. Chen, Xia Zhao, Y. Lin, Y. Huang, Qian Wang
Using a one-pot approach driven by the supramolecular interaction between β-cyclodextrin and adamantyl moieties, multifunctional viral nanoparticles can be facilely formulated for biomedical applications.
Supercharging: An Investigation Into The Effects Of External Amino Acid Residue Charge On The Solubility And Internal Electric Character Of Bound Ligands In A Heme-Binding De Novo-Designed Protein, 2013 CUNY City College
Supercharging: An Investigation Into The Effects Of External Amino Acid Residue Charge On The Solubility And Internal Electric Character Of Bound Ligands In A Heme-Binding De Novo-Designed Protein, Cooper French
Dissertations and Theses
De novo protein design offers many interesting prospects both as a means to better understand natural protein dynamics and as a potential resource in biomedical and industrial applications. In this work I describe the modification of a simple, well-characterized heme-binding protein by altering side chain residue identities on the hydrophilic surface of the protein to produce variants with a range of net external charges. These charge modifications had a significant impact on nearly every measurable character of the protein. This work establishes the hard limits of supercharging within our experimental protein scaffold system, demonstrating that excessive positive charge increased the ...
The Computation Of Cyclic Peptide With Prolin-Prolin Bond As Fusion Inhibitor Of Denv Envelope Protein Through Molecular Docking And Molecular Dynamics Simulation, Arli A. Parikesit, Hilyatuz Zahroh, Andreas S. Nugroho, Amalia Hapsari, Usman Sumo F. Tambunan
Arli A Parikesit
A disease that caused by dengue virus (DENV) has become the major health problem of the world. Nowadays, no effective treatment is available to overcome the disease due to the level of dengue virus pathogeneses. A novel treatment method such as antiviral drug is highly necessary for coping with the dengue disease. Envelope protein is one of the non-structural proteins of DENV, which engaged in the viral fusion process. It penetrates into the host cell to transfer its genetic material into the targeted cell followed by replication and establishment of new virus. Thus, the envelope protein can be utilized as ...
New Applications Of Mass Spectrometry For Drug And Lipid Analysis, 2012 University of Arkansas, Fayetteville
New Applications Of Mass Spectrometry For Drug And Lipid Analysis, Elizabeth Lauren Emerson
Theses and Dissertations
Mass spectrometry is an important tool used in many different disciplines and settings that include forensics, drug discovery, environmental analysis, and proteomics. Gas chromatography - mass spectrometry (GC-MS) and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI - TOF MS) are two of the most important instruments used for analysis of compounds. Chapters 1 and 2 of this discussion use GC-MS for the investigation of synthetic cannabinoids in `K2' incense products and the detection of metabolites in urine samples from individuals suspected of consuming these mixtures. Analytical standards were synthesized and used for identification and confirmation of structures. Detection of these ...
Activation Of Pad4 In Net Formation, 2012 The Scripps Research Institute
Activation Of Pad4 In Net Formation, Amanda S. Rohrbach, Daniel J. Slade, Paul R. Thompson, Kerri A. Mowen
Thompson Lab Publications
Peptidylarginine deiminases, or PADs, convert arginine residues to the non-ribosomally encoded amino acid citrulline in a variety of protein substrates. PAD4 is expressed in granulocytes and is essential for the formation of neutrophil extracellular traps (NETs) via PAD4-mediated histone citrullination. Citrullination of histones is thought to promote NET formation by inducing chromatin decondensation and facilitating the expulsion of chromosomal DNA that is coated with antimicrobial molecules. Numerous stimuli have been reported to lead to PAD4 activation and NET formation. However, how this signaling process proceeds and how PAD4 becomes activated in cells is largely unknown. Herein, we describe the various ...
Identification Of Padi2 As A Potential Breast Cancer Biomarker And Therapeutic Target., 2012 Cornell University
Identification Of Padi2 As A Potential Breast Cancer Biomarker And Therapeutic Target., John L. Mcelwee, Sunish Mohanan, Obi L. Griffith, Heike C. Breuer, Lynne J. Anguish, Brian D. Cherrington, Ashley M. Palmer, Louise R. Howe, Venkataraman Subramanian, Corey P. Causey, Paul R. Thompson, Joe W. Gray, Scott A. Coonrod
Thompson Lab Publications
BACKGROUND: We have recently reported that the expression of peptidylarginine deiminase 2 (PADI2) is regulated by EGF in mammary cancer cells and appears to play a role in the proliferation of normal mammary epithelium; however, the role of PADI2 in the pathogenesis of human breast cancer has yet to be investigated. Thus, the goals of this study were to examine whether PADI2 plays a role in mammary tumor progression, and whether the inhibition of PADI activity has anti-tumor effects.
METHODS: RNA-seq data from a collection of 57 breast cancer cell lines was queried for PADI2 levels, and correlations with known ...
Potential Role Of Peptidylarginine Deiminase Enzymes And Protein Citrullination In Cancer Pathogenesis., Sunish Mohanan, Brian D. Cherrington, Sachi Horibata, John L. Mcelwee, Paul R. Thompson, Scott A. Coonrod
Thompson Lab Publications
The peptidylarginine deiminases (PADs) are a family of posttranslational modification enzymes that catalyze the conversion of positively charged protein-bound arginine and methylarginine residues to the uncharged, nonstandard amino acid citrulline. This enzymatic activity is referred to as citrullination or, alternatively, deimination. Citrullination can significantly affect biochemical pathways by altering the structure and function of target proteins. Five mammalian PAD family members (PADs 1-4 and 6) have been described and show tissue-specific distribution. Recent reviews on PADs have focused on their role in autoimmune diseases. Here, we will discuss the potential role of PADs in tumor progression and tumor-associated inflammation. In ...
The Development Of Organometallic Oboc Peptide Libraries For Use In Molecular Imaging, 2012 The University of Western Ontario
The Development Of Organometallic Oboc Peptide Libraries For Use In Molecular Imaging, Dana R. Cruickshank
Electronic Thesis and Dissertation Repository
The discovery of peptide-based targeted imaging agents is hindered by the required addition of a radionuclide that can affect the binding properties of the peptide. Screening of peptide libraries is problematic because they do not take into account the presence of the imaging agent. Herein the development of an organometallic OBOC peptide library that contain a rhenium (I) tricarbonyl-based imaging entity surrogate incorporated directly onto the peptide chain, is reported. Additionally, MALDI tandem mass spectrometry is reported as a method to deconvolute organometallic peptides containing four different rhenium (I) tricarbonyl chelates on the N-terminus of octapeptides. Furthermore, an organometallic OBOC ...
Insights On Cytochrome P450 Enzymes And Inhibitors Obtained Through Qsar Studies, 2012 Xavier University of Louisiana
Insights On Cytochrome P450 Enzymes And Inhibitors Obtained Through Qsar Studies, Jayalakshmi Sridhar, Jiawang Liu, Maryam Foroozesh, Cheryl Stevens L. Klein Stevens
Faculty and Staff Publications
The cytochrome P450 (CYP) superfamily of heme enzymes play an important role in the metabolism of a large number of endogenous and exogenous compounds, including most of the drugs currently on the market. Inhibitors of CYP enzymes have important roles in the treatment of several disease conditions such as numerous cancers and fungal infections in addition to their critical role in drug-drug interactions. Structure activity relationships (SAR), and three-dimensional quantitative structure activity relationships (3D-QSAR) represent important tools in understanding the interactions of the inhibitors with the active sites of the CYP enzymes. A comprehensive account of the QSAR studies on ...
Reactions Of Methyl Perfluoroalkyl Ethers With Isopropyl Alcohol: Experimental And Theoretical Studies, 2012 University of Dayton
Reactions Of Methyl Perfluoroalkyl Ethers With Isopropyl Alcohol: Experimental And Theoretical Studies, Howard Knachel, Vladimir Benin, Chadwick Barklay, Janine C. Birkbeck, Billy D. Faubion, William E. Moddeman
Chemistry Faculty Publications
The reaction of an isomeric mixture of the methyl perfluoroalkyl ether, C4F9OCH3 (Novec-7100), in the presence of isopropyl alcohol (IPA) and/or water has been studied by measuring the rate of product formation using an ion-selective electrode (ISE) for fluoride ion, Karl Fisher coulometric titrations for water, and 1H and 19F NMR spectroscopy for product identification and rate studies. The results showed the methyl perfluoroalkyl ether to be very stable with products forming at the rate of ∼1 ppm per year at a laboratory temperature of 20 °C. Measurements over the temperature range of 6° to 100 °C were made ...
Characterization Of Polymeric Phthalocyanine Nanoparticles Using Dynamic Light Scattering Laser, 2012 Governors State University
Characterization Of Polymeric Phthalocyanine Nanoparticles Using Dynamic Light Scattering Laser, Satish Kumar Murarishetty
All Capstone Projects
The objective of this study is to use DLS (Dynamic Light Scattering) to analyze polymeric copper phthalocyanine nanoparticles (CuPcNPs). CuPcNPs are synthesized in order to facilitate drug penetration of bacterial biofilms for the treatment of chronic wounds. Microorganisms that reside inside the biofilms of chronic wounds are very resistant to any kind of treatment, even to the host’s own immune system. Therefore we have proposed an alternative method to destroy the microorganisms existing within the biofilms. Photodynamic anti-bacterial chemotherapy (PACT) has received much attention for the past decade due to the multi-drug resistant strands. PACT uses photons, a photosensitizer ...
Potential Role For Padi-Mediated Histone Citrullination In Preimplantation Development., 2012 Cornell University
Potential Role For Padi-Mediated Histone Citrullination In Preimplantation Development., Rui Kan, Mei Jin, Venkataraman Subramanian, Corey P. Causey, Paul R. Thompson, Scott A. Coonrod
Thompson Lab Publications
BACKGROUND: The peptidylarginine deiminases (PADIs) convert positively charged arginine residues to neutrally charged citrulline on protein substrates in a process that is known as citrullination or deimination. Previous reports have documented roles for histone citrullination in chromatin remodeling and gene regulation in several tissue types, however, a potential role for histone citrullination in chromatin-based activities during early embryogenesis has not been investigated.
RESULTS: In the present study, we tested by laser scanning confocal indirect immunofluorescence microscopy whether specific arginine residues on the histone H3 and H4 N-terminal tails (H4R3, H3R2 + 8 + 17, and H3R26) were citrullinated in mouse oocytes and ...
Design And Synthesis Studies Of New S-Adenosyl-L-Methionine Analogues, 2012 New Jersey Institute of Technology
Design And Synthesis Studies Of New S-Adenosyl-L-Methionine Analogues, Shanshan Wu
S-Adenosyl-L-Methionine (SAM), which is involved in methyl group transfers, is the second most abundant coenzyme in the human body. It is made from adenosinetriphosphate (ATP) and methionine catalyzed by adenosyltransferase. Methyltransferases (MTs) transfer the activated methyl group from the sulfur center to a specific position in a variety of substrates, e.g., DNA, RNA, proteins, and secondary metabolites. Methyltransferases have been linked to various diseases, including cancer.
In recent years, more and more SAM analogues have been reported. These analogues show activities in biological target labeling. The purpose of this thesis is to design and synthesize new SAM analogues to ...
Size Dependent Antimicrobial Properties Of Sugar Encapsulated Gold Nanoparticles, 2012 Western Kentucky University
Size Dependent Antimicrobial Properties Of Sugar Encapsulated Gold Nanoparticles, Lakshmisri Manisha Vangala
Masters Theses & Specialist Projects
The antimicrobial properties of dextrose encapsulated gold nanoparticles (dGNPs) with average diameters of 25 nm, 60 nm, and 120 nm (± 5 nm) synthesized by green chemistry principles were investigated against both Gram-negative and Gram-positive bacteria. Studies were performed involving the effect of the dGNPs on the growth, morphology and the ultrastructural properties of bacteria. dGNPs were found to have significant dose dependent antibacterial activity which was directly proportional to their size and also their concentration. The microbial assays revealed the dGNPs to be bacteriostatic as well as bactericidal. The dGNPs exhibited their bactericidal action through the disruption of the bacterial ...
Synthesis Of S-Ribosyl-L-Homocysteine And Analogs Modified At The Homocysteine-C3 Position, 2012 The University of San Francisco
Synthesis Of S-Ribosyl-L-Homocysteine And Analogs Modified At The Homocysteine-C3 Position, Ruoyi Liu
Quorum sensing (QS) is a process of bacterial cell-to-cell communication that conveys population density information in order to coordinate gene expression to produce synchronized behaviors. QS regulates the expression of virulence genes in many species of bacteria; hence, the manipulation of QS pathways may lead to treatment options against many bacterial diseases. The LuxS enzyme converts S-ribosyl-L-homocysteine (SRH) into homocysteine (HCys) and 4(S),5-dihydroxypentane-2,3-dione (DPD), which is the precursor of autoinducer-2 (AI-2). Thus, inhibitors of LuxS could prevent QS by halting the conversion of SRH to AI-2 rendering the cell “uncommunicative”. This work shows the successful chemical ...
Synthesis And Development Of Potential Cb1 Receptor Neutral Antagonists, 2012 University of New Orleans
Synthesis And Development Of Potential Cb1 Receptor Neutral Antagonists, Kimari Slaughter
University of New Orleans Theses and Dissertations
Cannabis and its derivatives have been used for both medicinal and recreational purposes. The study of this plant led to the discovery of over 60 cannabinoids, found exclusively in cannabis, that contribute to the behavioral effects of cannabis use, the most common is delta-9-tetrahydrocannabinol. Cannabinoid receptors function to increase activity in the mesolimbic dopamine reward system. Dopamine is a neurotransmitter that plays a major role in addition and its regulation plays a crucial role in mental and physical well-being. There is evidence that CB1 receptors are important to the reinforcing effects and the development of physical dependence on opiate ...
Synthesis And Biological Evaluation Of Rigid Analogues Of Methamphetamines, 2012 University of New Orleans
Synthesis And Biological Evaluation Of Rigid Analogues Of Methamphetamines, Andrea N. Forsyth
University of New Orleans Theses and Dissertations
A series of rigid azetidenyl-based methamphetamine analogs were synthesized from commercially available N-Boc-azetidinone. The benzylideneazetidine analogs were prepared via a Wittig olefination via the ylides generated from the corresponding triphenylphosphonium benzylhalide salts. The substituted benzylazetidine analogs were synthesized from the corresponding benzylideneazetidienes via hydrogention over palladium and platinum catalysts. The benzylideneazetidine and benzyliazetidine analogs were evaluated at monoamine transporters as a part of preliminary structure-activity study for the development of novel monoamine transporter ligands. The binding affinities of the azetidine analogs were determined at dopamine (DAT) and serotonin (SERT) transporters in rat brain tissue preparations. The preliminary in vitro ...
Interaction Of Pharmaceutical Compounds With Dissolved Organic Matter, 2012 Western Michigan University
Interaction Of Pharmaceutical Compounds With Dissolved Organic Matter, Carlen Nigl Smith
Highly prescribed pharmaceutical compounds and their metabolites used to treat conditions ranging from infections to depression are of environmental concern as these excreted compounds are increasingly appearing in wastewater influent. As pharmaceuticals enter treatment plants they also can interact with dissolved organic matter (DOM) present. This potential interaction between pharmaceutical compounds and DOM may inhibit the advanced oxidation process (AOP) based removal of drugs during water treatment. The focus of this research was to utilize multinuclear magnetic resonance and hydroxyl radical kinetics to assess whether an interaction between influent pharmaceuticals and DOM does occur, the extent of the interaction, and ...