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Spatial Mapping And Profiling Of Metabolite Distributions During Germination, Adam D. Feenstra, Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna D. Yandeau-Nelson, Basil J. Nikolau, Young-Jin Lee 2018 Iowa State University and Ames Laboratory

Spatial Mapping And Profiling Of Metabolite Distributions During Germination, Adam D. Feenstra, Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna D. Yandeau-Nelson, Basil J. Nikolau, Young-Jin Lee

Robbyn Anand

Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also ...


Genotypic Variation In Anthocyanins, Phenolic Compounds, And Antioxidant Activity In Cob And Husk Of Purple Field Corn, Ponsawan Khamphasan, Khomsorn Lomthaisong, Bhornchai Harakotr, Danupol Ketthaisong, Marvin Paul Scott, Kamol Lertrat, Bhalang Suriharn 2018 Khon Kaen University

Genotypic Variation In Anthocyanins, Phenolic Compounds, And Antioxidant Activity In Cob And Husk Of Purple Field Corn, Ponsawan Khamphasan, Khomsorn Lomthaisong, Bhornchai Harakotr, Danupol Ketthaisong, Marvin Paul Scott, Kamol Lertrat, Bhalang Suriharn

Agronomy Publications

Information on phytochemicals in the cob and husk of field corn is important for the use of corn waste in the production of value-added corn products. The objectives of this study were to evaluate the variation in monomeric anthocyanin content (MAC), total phenolic content (TPC), and antioxidant activity, as determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) in 53 purple field corn genotypes, and to study the correlations of these traits with color parameters. Fifty-three corn genotypes were planted in a randomized complete block design with three replications in two locations in the ...


Haplotype Loci Under Selection In Canadian Durum Wheat Germplasm Over 60 Years Of Breeding: Association With Grain Yield, Quality Traits, Protein Loss, And Plant Height, Amidou N'Diaye, Jemanesh K. Haile, Kirby T. Nilsen, Sean Walkowiak, Yuefeng Ruan, Asheesh K. Singh, Fran R. Clarke, John M. Clarke, Curtis J. Pozniak 2018 University of Saskatchewan

Haplotype Loci Under Selection In Canadian Durum Wheat Germplasm Over 60 Years Of Breeding: Association With Grain Yield, Quality Traits, Protein Loss, And Plant Height, Amidou N'Diaye, Jemanesh K. Haile, Kirby T. Nilsen, Sean Walkowiak, Yuefeng Ruan, Asheesh K. Singh, Fran R. Clarke, John M. Clarke, Curtis J. Pozniak

Agronomy Publications

Durum wheat was introduced in the southern prairies of western Canada in the late nineteenth century. Breeding efforts have mainly focused on improving quality traits to meet the pasta industry demands. For this study, 192 durum wheat lines were genotyped using the Illumina 90K Infinium iSelect assay, and resulted in a total of 14,324 polymorphic SNPs. Genetic diversity changed over time, declining during the first 20 years of breeding in Canada, then increased in the late 1980s and early 1990s. We scanned the genome for signatures of selection, using the total variance Fst-based outlier detection method (Lositan), the hierarchical ...


E3 Ubiquitin Ligases: Key Regulators Of Hormone Signaling In Plants, Dior R. Kelley 2018 Iowa State University

E3 Ubiquitin Ligases: Key Regulators Of Hormone Signaling In Plants, Dior R. Kelley

Dior Kelley

Ubiquitin-mediated control of protein stability is central to most aspects of plant hormone signaling. Attachment of ubiquitin to target proteins occurs via an enzymatic cascade with the final step being catalyzed by a family of enzymes known as E3 ubiquitin ligases, which have been classified based on their protein domains and structures. While E3 ubiquitin ligases are conserved among eukaryotes, in plants they are well-known to fulfill unique roles as central regulators of phytohormone signaling, including hormone perception and regulation of hormone biosynthesis. This review will highlight up-to-date findings that have refined well-known E3 ligase-substrate interactions and defined novel E3 ...


High Density Genetic Mapping Of Fusarium Head Blight Resistance Qtl In Tetraploid Wheat, Ehsan Sari, Samia Berraies, Ron E. Knox, Asheesh K. Singh, Yuefeng Ruan, Richard D. Cuthbert, Curtis J. Pozniak, Maria Antonia Henriquez, Santosh Kumar, Andrew J. Burt, Amidou N'Diaye, David J. Konkin, Adrian L. Cabral, Heather L. Campbell, Krystalee Wiebe, Janet Condie, Prabhath Lokuruge, Brad Meyer, George Fedak, John M. Clarke, Daryl J. Somers, Perre R. Fobert 2018 Agriculture and Agri-Food Canada

High Density Genetic Mapping Of Fusarium Head Blight Resistance Qtl In Tetraploid Wheat, Ehsan Sari, Samia Berraies, Ron E. Knox, Asheesh K. Singh, Yuefeng Ruan, Richard D. Cuthbert, Curtis J. Pozniak, Maria Antonia Henriquez, Santosh Kumar, Andrew J. Burt, Amidou N'Diaye, David J. Konkin, Adrian L. Cabral, Heather L. Campbell, Krystalee Wiebe, Janet Condie, Prabhath Lokuruge, Brad Meyer, George Fedak, John M. Clarke, Daryl J. Somers, Perre R. Fobert

Agronomy Publications

Breeding for Fusarium head blight (FHB) resistance in durum wheat is complicated by the quantitative trait expression and narrow genetic diversity of available resources. High-density mapping of the FHB resistance quantitative trait loci (QTL), evaluation of their co-localization with plant height and maturity QTL and the interaction among the identified QTL are the objectives of this study. Two doubled haploid (DH) populations, one developed from crosses between Triticum turgidum ssp. durum lines DT707 and DT696 and the other between T. turgidum ssp. durum cv. Strongfield and T. turgidum ssp. carthlicum cv. Blackbird were genotyped using the 90K Infinium iSelect chip ...


Color Space Standardization And Image Analysis For High-Throughput Phenotyping Of Sorghum Bicolor, Alexandria A. Pokorny 2018 Department of Mathematics, Illinois State University

Color Space Standardization And Image Analysis For High-Throughput Phenotyping Of Sorghum Bicolor, Alexandria A. Pokorny

Annual Symposium on Biomathematics and Ecology: Education and Research

No abstract provided.


Cis–Trans Controls And Regulatory Novelty Accompanying Allopolyploidization, Guanjing Hu, Jonathan F. Wendel 2018 Iowa State University

Cis–Trans Controls And Regulatory Novelty Accompanying Allopolyploidization, Guanjing Hu, Jonathan F. Wendel

Ecology, Evolution and Organismal Biology Publications

Allopolyploidy is a prevalent process in plants, having important physiological, ecological, and evolutionary consequences. Transcriptomic responses to genomic merger and doubling have been demonstrated in many allopolyploid systems, encompassing a diversity of phenomena including homoeolog expression bias, genome dominance, expression‐level dominance, and revamping of co‐expression networks. Notwithstanding the foregoing, there remains a need to develop a conceptual framework that will stimulate a deeper understanding of these diverse phenomena and their mechanistic interrelationships. Here we introduce considerations relevant to this framework with a focus on cistrans interactions among duplicated genes and alleles in hybrids and allopolyploids. By extending ...


Aac Viewfield Hard Red Spring Wheat, R. D. Cuthbert, R. M. DePauw, A. K. Singh, B. McCallum, T. Fetch 2018 Agriculture and Agri-Food Canada

Aac Viewfield Hard Red Spring Wheat, R. D. Cuthbert, R. M. Depauw, A. K. Singh, B. Mccallum, T. Fetch

Agronomy Publications

AAC Viewfield hard red spring wheat (Triticum aestivum L.) has grain yield significantly higher than the check cultivars Katepwa and Lillian and is similar to Carberry. AAC Viewfield matures significantly later than Katewpa and Lillian but is similar to Carberry. AAC Viewfield has an awned spike, a low lodging score indicative of strong straw, and significantly shorter plant stature than all checks. AAC Viewfield expressed resistance to prevalent races of yellow rust and stem rust, moderate resistance to leaf rust and common bunt, and intermediate resistance to Fusarium head blight. AAC Viewfield has quality attributes within the range of the ...


Multi-Objective Optimized Genomic Breeding Strategies For Sustainable Food Improvement, Deniz Akdemir, William D, Beavis, Roberto Fritsche-Neto, Asheesh K. Singh, Julio Isidro-Sánchez 2018 Cornell University

Multi-Objective Optimized Genomic Breeding Strategies For Sustainable Food Improvement, Deniz Akdemir, William D, Beavis, Roberto Fritsche-Neto, Asheesh K. Singh, Julio Isidro-Sánchez

Agronomy Publications

The purpose of breeding programs is to obtain sustainable gains in multiple traits while controlling the loss of genetic variation. The decisions at each breeding cycle involve multiple, usually competing, objectives; these complex decisions can be supported by the insights that are gained by applying multi-objective optimization principles to breeding. The discussion in this manuscript includes the definition of several multi-objective optimized breeding approaches within the phenotypic or genomic breeding frameworks and the comparison of these approaches with the standard multi-trait breeding schemes such as tandem selection, independent culling and index selection. Proposed methods are demonstrated with two empirical data ...


Perceptions Of Midwestern Crop Advisors And Growers On Foliar Fungicide Adoption And Use In Maize, P. D. Esker, D. A. Shah, C. A. Bradley, S. P. Conley, P. A. Paul, A. E. Robertson 2018 Penn State University

Perceptions Of Midwestern Crop Advisors And Growers On Foliar Fungicide Adoption And Use In Maize, P. D. Esker, D. A. Shah, C. A. Bradley, S. P. Conley, P. A. Paul, A. E. Robertson

Plant Pathology and Microbiology Publications

Foliar fungicide use in hybrid maize in the United States was rare before 2000. The decade from 2000 to 2010 saw foliar fungicides increasingly applied to maize in the absence of appreciable disease pressure, a practice seemingly at odds with integrated pest management philosophy. Yet, it is commonly believed that growers do not employ management strategies unless there are perceived benefits. Maize (corn) growers (CGs) and certified crop advisors (CCAs) across four Midwestern states (Iowa, Illinois, Ohio, and Wisconsin) were surveyed to better understand their practices, values and perceptions concerning the use of foliar fungicides during 2005 to 2009. The ...


Rethinking Invasion Impacts Across Multiple Field Sites Using European Swallowwort (Vincetoxicum Rossicum) As A Model Invader, Grant L. Thompson, Terrence H. Bell, Jenny Kao-Kniffin 2018 Cornell University

Rethinking Invasion Impacts Across Multiple Field Sites Using European Swallowwort (Vincetoxicum Rossicum) As A Model Invader, Grant L. Thompson, Terrence H. Bell, Jenny Kao-Kniffin

Grant Thompson

European swallowwort [Vincetoxicum rossicum (Kleopow) Barbarich] is found in the northeastern United States and southeastern Canada. It forms dense growth patterns that reduce plant and insect biodiversity, and lab assays show that it produces allelopathic compounds that affect microbial activity. Consequently, we hypothesized that V. rossicum alters soil microbiome composition and activity in invaded habitats, which may impact
ecosystem properties and processes. We sampled soil from a similar time point within a growing season at each of five sites in New York State where V. rossicum was both present and absent. We measured bacterial and fungal microbiome composition, available soil ...


Identification Of Quantitative Trait Loci (Qtl) For Tolerance To Metribuzin In Soft Red Winter Wheat (Triticum Aestivum L.), Hugo Alfonso Anzueto Ponciano 2018 Louisiana State University and Agricultural and Mechanical College

Identification Of Quantitative Trait Loci (Qtl) For Tolerance To Metribuzin In Soft Red Winter Wheat (Triticum Aestivum L.), Hugo Alfonso Anzueto Ponciano

LSU Master's Theses

Wheat is one of the world’s most important food crops and provides nearly 20% of the calories consumed. Wheat is the third most important crop after corn and rice on a global production scale. In Louisiana wheat harvest was about 6000 hectares in 2017. Wheat production is negatively affected by several biotic and abiotic factors including weather, diseases, and weeds. Metribuzin is a broad-spectrum herbicide commonly used in wheat because it is inexpensive and controls most of the common weeds in wheat fields. Metribuzin has benefits for the grower controlling weeds that might cause yield loss in varieties or ...


Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang 2018 Iowa State University

Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang

Food Science and Human Nutrition Publications

There has been a great interest in the phospholipids (PL) found in dairy products because of their health and functional properties. In this study, a technology that was originally developed for egg yolk PL extraction was applied to whey protein phospholipid concentrate (WPPC). This method successfully precipitated the proteins present in WPPC and extracted the lipids with a renewable alcoholic solvent, ethanol. The effect of ethanol concentration, extraction temperature, and extraction number on the recovery of total lipid, total PL, and individual PL class was evaluated. The optimum processing conditions for a combined 5-stage sequential extraction for producing a PL-enriched ...


Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen 2018 University of Calgary

Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen

The Goose

Review of Daniel Coleman's Yardwork: A Biography of an Urban Place.


Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow 2018 University of New Orleans

Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow

University of New Orleans Theses and Dissertations

Introduced populations of Eichhornia crassipes (Pontederiaceae) possess extremely low levels of genetic diversity due to severe bottleneck events and clonal reproduction. While populations elsewhere have been well studied, North American populations of E. crassipes remain understudied. We used Amplified Fragment Length Polymorphism markers to assess genetic diversity and population structure in North American E. crassipes populations. Patterns of diversity over the past fifty years were analyzed using herbarium specimens. Furthermore, we sampled populations across the Gulf Coast of the United States throughout a year to determine contemporary genetic diversity and assess potential seasonal effects. Genetic diversity was found to be ...


Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson 2018 Purdue

Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson

The Summer Undergraduate Research Fellowship (SURF) Symposium

An essential part of livestock diets is soybean meal, which is a major source of protein, but which also consists of antinutritional carbohydrates. Antinutritional carbohydrates such as raffinose and stachyose lead to irritation to the gut for monogastric livestock as well as unhealthy weight gain. A major objective of soybean genetics is to reduce these antinutritional carbohydrates within the seed and increase the levels of good carbohydrates. This will lead to healthier livestock and better meat quality. To select genes potentially responsible for variation in carbohydrate levels in seeds, the expression of genes encoding several biosynthetic enzymes was measured during ...


Heterosis-Related Genes Under Different Planting Densities In Maize, Juan Ma, Dengfeng Zhang, Yanyong Cao, Lifeng Wang, Jingjing Li, Thomas Lubberstedt, Tianyu Wang, Yu Li, Huiyong Li 2018 Henan Academy of Agricultural Sciences

Heterosis-Related Genes Under Different Planting Densities In Maize, Juan Ma, Dengfeng Zhang, Yanyong Cao, Lifeng Wang, Jingjing Li, Thomas Lubberstedt, Tianyu Wang, Yu Li, Huiyong Li

Agronomy Publications

Heterosis and increasing planting density have contributed to improving maize grain yield (GY) for several decades. As planting densities increase, the GY per plot also increases whereas the contribution of heterosis to GY decreases. There are trade-offs between heterosis and planting density, and the transcriptional characterization of heterosis may explain the mechanism involved. In this study, 48 transcriptome libraries were sequenced from four inbred Chinese maize lines and their F1 hybrids. They were planted at densities of 45,000 plants/ha and 67,500 plants/ha. Maternal-effect differentially expressed genes (DEGs) played important roles in processes related to photosynthesis and ...


Genetic Control And Geo-Climate Adaptation Of Pod Dehiscence Provide Novel Insights Into The Soybean Domestication And Expansion, Jiaoping Zhang, Asheesh K. Singh 2018 Iowa State University

Genetic Control And Geo-Climate Adaptation Of Pod Dehiscence Provide Novel Insights Into The Soybean Domestication And Expansion, Jiaoping Zhang, Asheesh K. Singh

Agronomy Publications

Loss of pod dehiscence is a key step during soybean [Glycine max (L.) Merr.] domestication. Genome-wide association analysis for soybean shattering identified loci harboring Pdh1, NST1A and SHAT1-5. Pairwise epistatic interactions were observed, and the dehiscent Pdh1 overcomes the resistance conferred by NST1A or SHAT1-5 locus, indicating that Pdh1 predominates pod dehiscence expression. Further candidate gene association analysis identified a nonsense mutation in NST1A associated with pod dehiscence. Allele composition and population differential analyses unraveled that Pdh1 and NST1A, but not SHAT1-5, underwent domestication and modern breeding selections. Geographic analysis showed that in Northeast China (NEC), indehiscence at both Pdh1 ...


Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada 2018 University of Arkansas, Fayetteville

Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada

Theses and Dissertations

Tools such as genome-wide association study (GWAS) and genomic selection (GS) have expedited the development of crops with improved genetic potential. While GWAS aims to identify significant markers associated with a trait of interest, the goal of GS is to utilize all marker effects to predict the performance of new breeding lines prior to testing. A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft winter wheat (SWW) lines evaluated in eight site-years in Arkansas and Oklahoma. Broad sense heritability of GY (H2=0.48) was moderate compared to other ...


Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani 2018 University of Arkansas, Fayetteville

Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani

Theses and Dissertations

Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function ...


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