Open Access. Powered by Scholars. Published by Universities.®

Plant Breeding and Genetics Commons

Open Access. Powered by Scholars. Published by Universities.®

2,200 Full-Text Articles 4,911 Authors 334,740 Downloads 75 Institutions

All Articles in Plant Breeding and Genetics

Faceted Search

2,200 full-text articles. Page 42 of 45.

Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott 2011 Iowa State University

Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott

Agronomy Publications

When grinding many corn kernels at once, there are several different options to choose from, but single kernel grinding is limited to few options with relatively low throughput. An attractive alternative is a reciprocating grinder with 3/8” grinding balls and a 4ml polycarbonate vial set. This format allows grinding of 24 samples simultaneously, which is a significant improvement to grinding throughput. A reciprocating grinder like the Talboys HT Homogenizer (Troemner, Thorofare, NJ), operates by violently shaking the 4ml polycarbonate vials containing a bearing and a single kernel. In our experience, stainless steel ball bearings do not lead to a ...


Candidate Gene Snp Discovery, Genotyping And Association With Wood Quality Traits In Eucalyptus Pilularis (Blackbutt), Timothy Russell Sexton 2011 Southern Cross University

Candidate Gene Snp Discovery, Genotyping And Association With Wood Quality Traits In Eucalyptus Pilularis (Blackbutt), Timothy Russell Sexton

Theses

This thesis uses association mapping to identify links between quantitative wood quality traits and Single Nucleotide Polymorphisms (SNPs) within wood formation genes. One priori expectation is that if a large panel of SNPs from candidate genes thought to influence associated wood properties are screened, some will be found that are associated with variation of phenotypes within the population and could be used for Marker assisted selection (MAS). Selecting trees based on their DNA sequence offers an alternative to traditional phenotypic selection for traits like wood quality, where breeding programs are largely constrained by the long generation times and the delay ...


Molecular And Phenotypic Comparisons Of Salt Effects On Soybean Cultivars With Differential Chloride Uptake Capacities, Sharon Faye Holifield, Fernando Ledesma Rodriguez, Richard D. Cartwright, Pengyin Chen, Kenneth Korth 2011 University of Arkansas, Fayetteville

Molecular And Phenotypic Comparisons Of Salt Effects On Soybean Cultivars With Differential Chloride Uptake Capacities, Sharon Faye Holifield, Fernando Ledesma Rodriguez, Richard D. Cartwright, Pengyin Chen, Kenneth Korth

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Genetic manipulation of crop plants, through breeding or transgenic approaches, for enhanced tolerance to abiotic stress holds great promise for improving yields and promoting new methods for sustainable agriculture. This study examines the potential role that genes of the soybean, Glycine max L., encoding elongation factor-1 alpha (EF-1α) and glyoxalase I (GlxI) might play in response to salt stress. Previous reports have suggested a possible function for both GlxI and EF-1α in conferring enhanced salt tolerance in other plant species. In addition to other possible mechanisms, salt tolerance in soybeans can be regulated by plant uptake and transport of chloride ...


A Plant Dj-1 Homolog Is Essential For Arabidopsis Thaliana Chloroplast Development, Jiusheng Lin, Tara J. Nazarenus, Jeanine L. Frey, Xinwen Liang, Mark A. Wilson, Julie M. Stone 2011 University of Nebraska-Lincoln

A Plant Dj-1 Homolog Is Essential For Arabidopsis Thaliana Chloroplast Development, Jiusheng Lin, Tara J. Nazarenus, Jeanine L. Frey, Xinwen Liang, Mark A. Wilson, Julie M. Stone

Faculty Publications from the Center for Plant Science Innovation

Protein superfamilies can exhibit considerable diversification of function among their members in various organisms. The DJ-1 superfamily is composed of proteins that are principally involved in stress response and are widely distributed in all kingdoms of life. The model flowering plant Arabidopsis thaliana contains three close homologs of animal DJ-1, all of which are tandem duplications of the DJ-1 domain. Consequently, the plant DJ-1 homologs are likely pseudo-dimeric proteins composed of a single polypeptide chain. We report that one A. thaliana DJ-1 homolog (AtDJ1C) is the first DJ-1 homolog in any organism that is required for viability. Homozygous disruption of ...


Structure Function Analysis Of An Adp-Ribosyltransferase Type Iii Effector And Its Rna-Binding Target In Plant Immunity, Byeong-Ryool Jeong, Yan Lin, Anna Joe, Ming Guo, Christin Korneli, Huirong Yang, Ping Wang, Min Yu, Ronald Cerny, Dorothee Staiger, James R. Alfano, Yanhui Xu 2011 University of Nebraska - Lincoln

Structure Function Analysis Of An Adp-Ribosyltransferase Type Iii Effector And Its Rna-Binding Target In Plant Immunity, Byeong-Ryool Jeong, Yan Lin, Anna Joe, Ming Guo, Christin Korneli, Huirong Yang, Ping Wang, Min Yu, Ronald Cerny, Dorothee Staiger, James R. Alfano, Yanhui Xu

Faculty Publications from the Center for Plant Science Innovation

Background: HopU1 ADP-ribosylates GRP7, suppressing plant immunity.

Results: The HopU1 structure has two novel loops required for GRP7 recognition, and HopU1 ribosylates GRP7 at an arginine in position 49 disrupting its function.

Conclusion: HopU1 targets a conserved arginine in GRP7, disabling its ability to bind immunity-related RNA.

Significance: The mechanistic details of how HopU1 recognizes its substrate reveal how HopU1 contributes to pathogenesis.


Structural And Functional Analysis Of The Type Iii Secretion System From Pseudomonas Fluorescens Q8r1-96, Dmitri V. Mavrodi, Anna Joe, Olga V. Mavrodi, Karl A. Hassan, David M. Weller, Ian T. Paulsen, Joyce E. Loper, James R. Alfano, Linda S. Thomashow 2011 Washington State University

Structural And Functional Analysis Of The Type Iii Secretion System From Pseudomonas Fluorescens Q8r1-96, Dmitri V. Mavrodi, Anna Joe, Olga V. Mavrodi, Karl A. Hassan, David M. Weller, Ian T. Paulsen, Joyce E. Loper, James R. Alfano, Linda S. Thomashow

Faculty Publications from the Center for Plant Science Innovation

Pseudomonas fluorescens Q8r1-96 represents a group of rhizosphere strains responsible for the suppressiveness of agricultural soils to take-all disease of wheat. It produces the antibiotic 2,4-diacetylphloroglucinol and aggressively colonizes the roots of cereal crops. In this study, we analyzed the genome of Q8r1-96 and identified a type III protein secretion system (T3SS) gene cluster that has overall organization similar to that of the T3SS gene cluster of the plant pathogen Pseudomonas syringae. We also screened a collection of 30 closely related P. fluorescens strains and detected the T3SS genes in all but one of them. The Q8r1-96 genome contained ...


Two Distinct Roles Of Arabidopsis Homolog Of Trithorax1 (Atx1) At Promoters And Within Transcribed Regions Of Atx1-Regulated Genes, Yong Ding, Zoya Avramova, Michael E. Fromm 2011 University of Nebraska - Lincoln

Two Distinct Roles Of Arabidopsis Homolog Of Trithorax1 (Atx1) At Promoters And Within Transcribed Regions Of Atx1-Regulated Genes, Yong Ding, Zoya Avramova, Michael E. Fromm

Faculty Publications from the Center for Plant Science Innovation

The Arabidopsis thaliana trithorax-like protein, ATX1, shares common structural domains, has similar histone methyltransferase (HMT) activity, and belongs in the same phylogenetic subgroup as its animal counterparts. Most of our knowledge of the role of HMTs in trimethylating lysine 4 of histone H3 (H3K4me3) in transcriptional regulation comes from studies of yeast and mammalian homologs. Little is known about the mechanism by which ATX1, or any other HMT of plant origin, affects transcription. Here, we provide insights into how ATX1 influences transcription at regulated genes, playing two distinct roles. At promoters, ATX1 is required for TATA binding protein (TBP) and ...


Mpk6, Sphinganine And The Lcb2a Gene From Serine Palmitoyltransferase Are Required In The Signaling Pathway That Mediates Cell Death Induced By Long Chain Bases In Arabidopsis, Mariana Saucedo-Garcia, Arturo Guevara-Garcia, Ariadna Gonzalez-Solis, Felipe Cruz-Garcia, Sonia Vazquez-Santana, Jonathan E. Markham, M. Guadalupe Lozano-Rosas, Charles R. Dietrich, Maricela Ramos-Vega, Edgar B. Cahoon, Marina Gavilanes-Ruiz 2011 Universidad Nacional Autonoma de Mexico

Mpk6, Sphinganine And The Lcb2a Gene From Serine Palmitoyltransferase Are Required In The Signaling Pathway That Mediates Cell Death Induced By Long Chain Bases In Arabidopsis, Mariana Saucedo-Garcia, Arturo Guevara-Garcia, Ariadna Gonzalez-Solis, Felipe Cruz-Garcia, Sonia Vazquez-Santana, Jonathan E. Markham, M. Guadalupe Lozano-Rosas, Charles R. Dietrich, Maricela Ramos-Vega, Edgar B. Cahoon, Marina Gavilanes-Ruiz

Faculty Publications from the Center for Plant Science Innovation

• Long chain bases (LCBs) are sphingolipid intermediates acting as second messengers in programmed cell death (PCD) in plants. Most of the molecular and cellular features of this signaling function remain unknown.

• We induced PCD conditions in Arabidopsis thaliana seedlings and analyzed LCB accumulation kinetics, cell ultrastructure and phenotypes in serine palmitoyltransferase (spt), mitogen-activated protein kinase (mpk), mitogenactivated protein phosphatase (mkp1) and lcb-hydroxylase (sbh) mutants.

• The lcb2a-1 mutant was unable to mount an effective PCD in response to fumonisin B1 (FB1), revealing that the LCB2a gene is essential for the induction of PCD. The accumulation kinetics of LCBs in wild-type (WT ...


Letter From The Dean, Michael Vayda 2011 University of Arkansas, Fayetteville

Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


A Novel Gene Silencing Vector For Plant Genomics And Biotechnology, Dustin Hoover, Scott Nicholson, Vibha Srivastava 2011 University of Arkansas, Fayetteville

A Novel Gene Silencing Vector For Plant Genomics And Biotechnology, Dustin Hoover, Scott Nicholson, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene silencing is a process of suppressing activity of specific genes by producing “interfering” RNA encoded by foreign genes. This process serves as the principle of genetic modification in plants and animals, which is an important tool in genomics and biotechnology, allowing scientists to manipulate organisms to better meet human demands. New approaches of gene silencing may enable improvements on current practices of genetic modification, and broaden the application and impact of gene silencing in biotechnology. Recently, a novel vector design consisting of the transcription of short gene fragments lacking transcription termination signals was demonstrated to be effective in partial ...


Influence Of The Rag1 And Rag2 Genes On Aphid Resistance And Agronomic Performance Of Soybean Lines, Shaylyn L. Wiarda, Walter R. Fehr, Matthew E. O'Neal 2010 Iowa State University

Influence Of The Rag1 And Rag2 Genes On Aphid Resistance And Agronomic Performance Of Soybean Lines, Shaylyn L. Wiarda, Walter R. Fehr, Matthew E. O'Neal

Proceedings of the Integrated Crop Management Conference

Aphids have been causing economic damage to soybean in the United States since 2000 (Hartman et al., 2001). High aphid density on soybeans causes the yield to decrease due to plant damage including leaf puckering, plant stunting, reduced pod and/or seed counts, and smaller seeds (Rice et al., 2008). In addition, the black sooty mold fungus that grows on aphid honeydew has a negative influence on soybean performance by reducing photosynthesis (Beckendorf et al., 2008).


Breeding Sorghum Cultivars For Processing, Gary Perterson 2010 Texas A & M University

Breeding Sorghum Cultivars For Processing, Gary Perterson

INTSORMIL Presentations

Discusses the diversity of traits in sorghum and details some aspects of breeding techniques.


Evaluation Of Novel Input Output Traits In Sorghum Through Biotechnology, Tejinder K. Mall 2010 University of Nebraska-Lincoln

Evaluation Of Novel Input Output Traits In Sorghum Through Biotechnology, Tejinder K. Mall

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop world-wide as well as an important source of feed, fiber and biofuel. It is a C4 plant and is well adapted to environments subject to high temperature and water limitation. Despite these agronomic qualities, it suffers from limitations of sensitivity to low temperature and the grain has drawbacks in relation to functionality and digestibility. The objectives of this study are: 1. To promote seed germination at low temperature and to enhance the seedling cold tolerance. 2. To enhance the grain digestibility and functionality. In an attempt to augment ...


Detection Of Soybean Seed Protein Qtls Using Selective Genotyping, Piyaporn Phansak 2010 University of Nebraska-Lincoln

Detection Of Soybean Seed Protein Qtls Using Selective Genotyping, Piyaporn Phansak

Theses, Dissertations, and Student Research in Agronomy and Horticulture

A quantitative trait locus (QTL) is a statically defined location of a gene governing that trait. QTL identification is the first step towards using marker-assisted selection (MAS) to introgress desirable QTL alleles into elite high-yield cultivars. Hundreds of high protein plant introductions (PIs) exist in the USDA germplasm collection and are a source of high protein alleles. Although 86 protein QTLs are currently listed in SoyBase, many are likely repeat discoveries of the same QTL(s), given the typical +/- 10 cM confidence intervals associated with QTL positions. Six germplasm accessions of maturity groups (MGs) II to IV that exhibited high ...


High-Resolution Genotyping Via Whole Genome Hybridizations To Microarrays Containing Long Oligonucleotide Probes, Yan Fu, Nathan M. Springer, Kai Ying, Cheng-Ting Yeh, A. Leonardo Iniguez, Todd Richmond, Wei Wu, Brad Barbazuk, Dan Nettleton, Jeff Jeddeloh, Patrick S. Schnable 2010 Iowa State University

High-Resolution Genotyping Via Whole Genome Hybridizations To Microarrays Containing Long Oligonucleotide Probes, Yan Fu, Nathan M. Springer, Kai Ying, Cheng-Ting Yeh, A. Leonardo Iniguez, Todd Richmond, Wei Wu, Brad Barbazuk, Dan Nettleton, Jeff Jeddeloh, Patrick S. Schnable

Statistics Publications

To date, microarray-based genotyping of large, complex plant genomes has been complicated by the need to perform genome complexity reduction to obtain sufficiently strong hybridization signals. Genome complexity reduction techniques are, however, tedious and can introduce unwanted variables into genotyping assays. Here, we report a microarray-based genotyping technology for complex genomes (such as the 2.3 GB maize genome) that does not require genome complexity reduction prior to hybridization. Approximately 200,000 long oligonucleotide probes were identified as being polymorphic between the inbred parents of a mapping population and used to genotype two recombinant inbred lines. While multiple hybridization replicates ...


Regalia-Induced Resistance In Powdery Mildew-Infected Grapevine Plants Correlates With The Induction Of Chalcone Synthase, Thomas Crottogini 2010 California Polytechnic State University - San Luis Obispo

Regalia-Induced Resistance In Powdery Mildew-Infected Grapevine Plants Correlates With The Induction Of Chalcone Synthase, Thomas Crottogini

Horticulture and Crop Science

No abstract provided.


An Improved Tissue Culture And Transformation System For Switchgrass (Panicum Virgatum L.), Jason Neil Burris 2010 University of Tennessee, Knoxville

An Improved Tissue Culture And Transformation System For Switchgrass (Panicum Virgatum L.), Jason Neil Burris

Masters Theses

Switchgrass (Panicum virgatum), a summer perennial grass native to North America, is currently being explored for its potential use in the production of biofuels. With these interests, genetic manipulation of switchgrass to produce plants that are easier to digest, have an increased resistance to diseases and stresses, and maintain viability longer in the field are required. Therefore, it was necessary to develop a reliable and efficient tissue culture system for the transformation of switchgrass. Current switchgrass tissue culture requires months for regeneration of transformants with relatively poor transformation efficiencies and are limited to derivatives of a single variety, Alamo. We ...


Identification Of Novel Brown Midrib Genes In Maize By Tests Of Allelism, F. Ali, P. Scott, J. Bakht, Y. Chen, T. Lubberstedt 2010 Pukhtunkhwa Agricultural University

Identification Of Novel Brown Midrib Genes In Maize By Tests Of Allelism, F. Ali, P. Scott, J. Bakht, Y. Chen, T. Lubberstedt

Agronomy Publications

Brown midrib (bm) mutations are known to affect cell-wall digestibility by altering the quantity and composition of lignins in cell walls, resulting in higher ethanol yield and increased cell-wall digestibility. So far, four bm genes (bm1, bm2, bm3 and bm4) were identified and mapped in maize, the last one (bm4) in 1947. In this study, 13 spontaneous mutations (bm*A–M) resulting in the appearance of brown midribs were crossed with bm1–4 for tests of allelism. From these tests, we report two new bm mutants bm5 (bm*F) and bm6 (bm ...


Fusarium Head Blight: Winter Wheat Cultivar Responses And Characterization Of Pathogen Isolates, John Fredy Hernandez Nopsa 2010 University of Nebraska-Lincoln

Fusarium Head Blight: Winter Wheat Cultivar Responses And Characterization Of Pathogen Isolates, John Fredy Hernandez Nopsa

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Fusarium head blight (FHB) is a destructive disease of wheat (Triticum aestivum L). FHB reduces yield and grain quality and causes accumulation of the mycotoxin deoxynivalenol (DON) in grain. Cultivar resistance is one of the most effective management strategies for FHB. Experiments were conducted to 1) identify winter wheat cultivars with resistance to FHB and DON accumulation, 2) determine the effect of winter wheat cultivar on the relationship between FHB and DON concentration, and 3) identify the major species of Fusarium causing FHB in Nebraska and characterize its isolates. Differences (P ≤ 0.05) were detected among cultivars in FHB index ...


More Than 800 Scn-Resistant Soybean Varieties Available To Iowa Growers, Gregory L. Tylka 2010 Iowa State University

More Than 800 Scn-Resistant Soybean Varieties Available To Iowa Growers, Gregory L. Tylka

Integrated Crop Management News

Soybean varieties that are resistant to the soybean cyst nematode (SCN) are extremely important for managing this damaging pest. In general, SCN-resistant soybean varieties yield greater than susceptible varieties in SCN-infested fields, and resistant varieties keep SCN population densities from increasing, which ensures profitable soybean production in future years.


Digital Commons powered by bepress