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Canavalia And Dolichos Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez 2019 University of Nebraska - Lincoln

Canavalia And Dolichos Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Botanical repellents and pesticides are now being rediscovered as new tools for integrated pest management in order to reduce the use of toxic chemicals in crop production. Canavalia gladiata and Dolichos lablab are two Fabaceae very well adapted to farmlands of El Salvador, effective as living barriers and mostly as cover crops, however, they are not yet very well disseminated. This document describes the potential for using the liquid extracts and the dry flour of raw seeds of those plants for economic benefit and practical convenience for pest management in Salvadorian agriculture under field conditions. Seed extracts were useful when ...


Sheet‐Extruded Films From Blends Of Hydroxypropylated And Native Corn Starches, And Their Characterization, Hee-Young Kim, Buddhi P. Lamsal, Jay-lin Jane, David A. Grewell 2019 Iowa State University

Sheet‐Extruded Films From Blends Of Hydroxypropylated And Native Corn Starches, And Their Characterization, Hee-Young Kim, Buddhi P. Lamsal, Jay-Lin Jane, David A. Grewell

Jay-Lin Jane

Sheet‐extruded films from the blends of hydroxypropylated normal corn starch (HP) and native normal corn starch (NS) at weight ratios of 100:0, 90:10, 70:30, 50:50, 30:70, and 0:100 were prepared and characterized. Glycerol and water were added as plasticizers at 11 and 27% starch weight, respectively. The highest tensile strength (TS) and longest elongation to break (EB) for dry films were observed at 70:30 HP: NS ratio, which was 25.76 MPa, and 3.97%, respectively. However, TS and EB of this 70:30 blend extruded starch film exhibited low resistance upon ...


Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. McDaniel, D. Raj Raman, Sharon L. Weyers 2019 Iowa State University

Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. Mcdaniel, D. Raj Raman, Sharon L. Weyers

Douglas L Karlen

The Midwestern U.S. landscape is one of the most highly altered and intensively managed ecosystems in the country. The predominant crops grown are maize (Zea mays L.) and soybean [Glycine max (L.) Merr]. They are typically grown as monocrops in a simple yearly rotation or with multiple years of maize (2 to 3) followed by a single year of soybean. This system is highly productive because the crops and management systems have been well adapted to the regional growing conditions through substantial public and private investment. Furthermore, markets and supporting infrastructure are highly developed for both crops. As maize ...


Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. McDaniel, D. Raj Raman, Sharon L. Weyers 2019 Iowa State University

Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. Mcdaniel, D. Raj Raman, Sharon L. Weyers

D. Raj Raman

The Midwestern U.S. landscape is one of the most highly altered and intensively managed ecosystems in the country. The predominant crops grown are maize (Zea mays L.) and soybean [Glycine max (L.) Merr]. They are typically grown as monocrops in a simple yearly rotation or with multiple years of maize (2 to 3) followed by a single year of soybean. This system is highly productive because the crops and management systems have been well adapted to the regional growing conditions through substantial public and private investment. Furthermore, markets and supporting infrastructure are highly developed for both crops. As maize ...


Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. McDaniel, D. Raj Raman, Sharon L. Weyers 2019 Iowa State University

Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. Mcdaniel, D. Raj Raman, Sharon L. Weyers

Amy L. Kaleita

The Midwestern U.S. landscape is one of the most highly altered and intensively managed ecosystems in the country. The predominant crops grown are maize (Zea mays L.) and soybean [Glycine max (L.) Merr]. They are typically grown as monocrops in a simple yearly rotation or with multiple years of maize (2 to 3) followed by a single year of soybean. This system is highly productive because the crops and management systems have been well adapted to the regional growing conditions through substantial public and private investment. Furthermore, markets and supporting infrastructure are highly developed for both crops. As maize ...


Sheet‐Extruded Films From Blends Of Hydroxypropylated And Native Corn Starches, And Their Characterization, Hee-Young Kim, Buddhi P. Lamsal, Jay-lin Jane, David A. Grewell 2019 Iowa State University

Sheet‐Extruded Films From Blends Of Hydroxypropylated And Native Corn Starches, And Their Characterization, Hee-Young Kim, Buddhi P. Lamsal, Jay-Lin Jane, David A. Grewell

Food Science and Human Nutrition Publications

Sheet‐extruded films from the blends of hydroxypropylated normal corn starch (HP) and native normal corn starch (NS) at weight ratios of 100:0, 90:10, 70:30, 50:50, 30:70, and 0:100 were prepared and characterized. Glycerol and water were added as plasticizers at 11 and 27% starch weight, respectively. The highest tensile strength (TS) and longest elongation to break (EB) for dry films were observed at 70:30 HP: NS ratio, which was 25.76 MPa, and 3.97%, respectively. However, TS and EB of this 70:30 blend extruded starch film exhibited low resistance upon ...


Negative Impacts Of The Beef Industry: Lab-Grown Meat, Stephanie Grass 2019 Bowling Green State University

Negative Impacts Of The Beef Industry: Lab-Grown Meat, Stephanie Grass

WRIT: Journal of First-Year Writing

The beef industry is harmful to the environment and human health and alternative solutions must be implemented in order to mitigate the effects of climate change. Water and grain are used in agriculture in abundance despite the negative environmental effects it causes. Cattle are the biggest contributors to greenhouse gas emissions in the sector, also contributing to climate change. Antibiotics are used in large quantities without regard to potential future consequences. One potential solution for this problem is lab-grown beef, which demands very little from the consumer and would take pressure off the environmental issues the beef industry creates. Lab-grown ...


Qqs Orphan Gene And Its Interactor Nf‐Yc4 Reduce Susceptibility To Pathogens And Pests, Mingsheng Qi, Wenguang Zheng, Xuefeng Zhao, Jessica D. Hohenstein, Yuba Kandel, Seth O’Conner, Yifan Wang, Chuanlong Du, Dan Nettleton, Gustavo C. Macintosh, Gregory L. Tylka, Eve Syrkin Wurtele, Steven A. Whitham, Ling Li 2019 Iowa State University

Qqs Orphan Gene And Its Interactor Nf‐Yc4 Reduce Susceptibility To Pathogens And Pests, Mingsheng Qi, Wenguang Zheng, Xuefeng Zhao, Jessica D. Hohenstein, Yuba Kandel, Seth O’Conner, Yifan Wang, Chuanlong Du, Dan Nettleton, Gustavo C. Macintosh, Gregory L. Tylka, Eve Syrkin Wurtele, Steven A. Whitham, Ling Li

Ling Li

Enhancing the nutritional quality and disease resistance of crops without sacrificing productivity is a key issue for developing varieties that are valuable to farmers and for simultaneously improving food security and sustainability. Expression of the Arabidopsis thaliana species‐specific AtQQS (Qua‐Quine Starch) orphan gene or its interactor, NF‐YC4 (Nuclear Factor Y, subunit C4), has been shown to increase levels of leaf/seed protein without affecting the growth and yield of agronomic species. Here, we demonstrate that overexpression of AtQQS and NF‐YC4 in Arabidopsis and soybean enhances resistance/reduces susceptibility to viruses, bacteria, fungi, aphids, and soybean cyst ...


Expression Of Multi-Domain Lytic Peptide Genes In Transgenic Plants For Disease Resistance, George Biliarski 2019 University of Nebraska - Lincoln

Expression Of Multi-Domain Lytic Peptide Genes In Transgenic Plants For Disease Resistance, George Biliarski

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Four non-plant multi-domain lytic peptide genes coding for antimicrobial peptides were expressed in Nicotiana benthamiana plants and tested against three fungal pathogens: Sclerotinia sclerotiorum, Rhizoctonia solani, and Pythium sp. Detached-leaf bioassay was performed for the transgenic plants carrying multi-domain lytic peptide constructs and compared with transgenic and wild type control plants. Symptom area of each leaf was measured with high precision using the Compu-Eye software and processed by SAS statistical package. The transgenic lines ORF13 and RSL1 showed substantial resistance to Sclerotinia sclerotiorum infection producing significantly smaller lesion areas compared to control plants. However, these lines were not ...


Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle 2019 University of Nebraska-Lincoln

Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Switchgrass (Panicum virgatum L.) is a perennial warm-season monocot that is indigenous to locations in North America east of the Rocky Mountains, and is considered a model grass for biofuel feedstock production. As switchgrass production increases, diseases pose a potential threat to biomass production and ethanol extraction. The two predominant switchgrass diseases in Nebraska are rust caused by Puccinia spp. and a viral mosaic disease caused by Panicum mosaic virus (PMV) and its associated Satellite panicum mosaic virus (SPMV). In this thesis, one study determined how SPMV affects PMV infection and systemic spread in two populations of switchgrass at different ...


Investigating Parental Effects On End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.) Hybrids, Anthony Delaney 2019 University of Nebraska-Lincoln

Investigating Parental Effects On End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.) Hybrids, Anthony Delaney

Theses, Dissertations, and Student Research in Agronomy and Horticulture

To optimize the performance and marketing of hybrid wheat, breeders need to understand the impact parents have on end-use quality. The goal of this study was to investigate the inheritance of end-use quality traits of hard winter wheat reciprocal hybrids produced by Easterly (2017). The 2016 analysis included 71 reciprocal hybrid combinations from 13 parents and the 2017 analysis included 79 reciprocal hybrid combinations from 14 parents. The reciprocals were composed of crosses between the top performing and bottom performing parents with respect to end-use quality as quantified by a Mixograph, a SDS sedimentation assay, and a SDS-SRC hybrid assay ...


An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana de Carvalho 2019 University of Nebraska-Lincoln

An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana De Carvalho

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The C4 photosynthetic pathway was first described over 50 years ago. Today, it is known that C4 evolved independently > 60 in plant lineages, which involves understanding not only the genetic, but also the metabolic features and differences involved in this process. Also, several adaptations are involved in the evolution of this type of photosynthesis, for example: changes in leaf anatomy and the evolution of kranz anatomy, physiology and metabolic pathways. In order to further investigate this pathway, different technologies and methods have been developed to unravel genes involved in C4 photosynthesis. With the advances in molecular biology and bioinformatics tools ...


Hormonal Signaling Induced In Soybean By Lysobacter Enzymogenes Strain C3, Jessica C. Walnut 2019 University of Nebraska-Lincoln

Hormonal Signaling Induced In Soybean By Lysobacter Enzymogenes Strain C3, Jessica C. Walnut

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The biological control bacterium Lysobacter enzymogenes strain C3 has been shown to suppress fungal diseases by producing a suite of lytic enzymes and antimicrobial secondary metabolites. Previous studies have found that C3, when applied to grass and cereal plants, also is capable of inducing local and systemic resistance against fungal pathogens. It is unknown, however, whether the bacterium has the ability to induce resistance in dicots and what signaling pathways are involved. This study assessed the ability of C3 to trigger local and systemic induced resistance responses in soybean (Glycine max ‘Williams82’) by analyzing relative expression of salicylic acid (SA ...


New Approaches To Use Genomics, Field Traits, And High-Throughput Phenotyping For Gene Discovery In Maize (Zea Mays), Zhikai Liang 2019 University of Nebraska-Lincoln

New Approaches To Use Genomics, Field Traits, And High-Throughput Phenotyping For Gene Discovery In Maize (Zea Mays), Zhikai Liang

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Maize is one of major crop species over the world. With lots of genetic resources and genomic tools, maize also serves as a model species to understand genetic diversity, facilitate the development of trait extraction algorithms and map candidate functional genes. Since the first version of widely used B73 reference genome was released, independent research groups in the maize community propagated seeds themselves for further research purposes. However, unexpected or occasional contamination may happen during this process. The first study in this thesis used public RNA-seq data of B73 from 27 research groups across three countries for calling single nucleotide ...


Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan 2019 University of Nebraska - Lincoln

Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Cereals are main food sources for humans and animals. However, during storage, cereal grains can be infested by insects and fungi. One of the most important insect storage pests is Sitophilus granarius (L., Coleoptera: Curculionidae). Adults and larvae can cause serious grain losses. In addition to insect pests, fungal pathogens may also invade the grain and cause economic loss, including contamination with mycotoxins, which threaten mammal health by causing serious disease. The most common mycotoxigenic grain fungi are species that belong to the genera Fusarium, Aspergillus and Penicillium. Currently, the most commonly used management strategies for insect and fungal storage ...


Impacts Of Size Fractionation And Processing On Functional Characteristics Of Broken Rice Kernels, Rebecca Mawusi Bruce 2019 University of Arkansas, Fayetteville

Impacts Of Size Fractionation And Processing On Functional Characteristics Of Broken Rice Kernels, Rebecca Mawusi Bruce

Theses and Dissertations

Rice flour generated from broken rice (brokens) has inconsistencies in functional properties. This may be due to differences in size and composition of brokens used for the flour. It is postulated that size classification of brokens can improve flour functionality. This study sought to investigate the effect of size fractionation of brokens on the functional or pasting properties of resulting rice flour. Broken rice was generated from six cultivars of freshly harvested rough rice that were dried at 25◦C in the laboratory. The brokens were classified into large, medium and small, using US sieve size 10, 12 and 20 ...


A Sugarcane Mosaic Virus Vector For Gene Expression In Maize, Yu Mei, Guanjun Liu, Chunquan Zhang, John H. Hill, Steven A. Whitham 2019 Iowa State University

A Sugarcane Mosaic Virus Vector For Gene Expression In Maize, Yu Mei, Guanjun Liu, Chunquan Zhang, John H. Hill, Steven A. Whitham

Plant Pathology and Microbiology Publications

Zea mays L. ssp. mays (maize) is an important crop plant as well as model system for genetics and plant biology. The ability to select among different virus‐based platforms for transient gene silencing or protein expression experiments is expected to facilitate studies of gene function in maize and complement experiments with stable transgenes. Here, we describe the development of a sugarcane mosaic virus (SCMV) vector for the purpose of protein expression in maize. An infectious SCMV cDNA clone was constructed, and heterologous genetic elements were placed between the protein 1 (P1) and helper component‐proteinase (HC‐Pro) cistrons in ...


Understanding Anhydrous Ammonia Application In Soil, John E. Sawyer 2019 Iowa State University

Understanding Anhydrous Ammonia Application In Soil, John E. Sawyer

John E. Sawyer

What happens when anhydrous ammonia is injected into soil?

Several physical and chemical reactions take place following anhydrous ammonia injection: dissolution in water, reaction with soil organic matter and clay, and attachment of ammonium ions on the soil cation exchange complex. These reactions all tend to limit the movement of ammonia, with water having the greatest initial effect. The highest concentration of ammonia is at/near the point of injection, with a tapering of the concentration toward the outer edge of the retention zone. Usually the greatest ammonia concentration is within the first inch or two of the injection point ...


Flood Adulterated Grain - Nutrient Supply To Crops When Land Applied, John E. Sawyer, Antonio Mallarino 2019 Iowa State University

Flood Adulterated Grain - Nutrient Supply To Crops When Land Applied, John E. Sawyer, Antonio Mallarino

John E. Sawyer

Flooding has been extensive in several areas of Iowa this spring. In some cases, stored grain has been affected by flood waters. Land application of flood adulterated grain as a nutrient source for a future crop may be an option for some. See the Iowa Dept. of Natural Resources (IDNR) and Iowa Dept. of Agriculture and Land Stewardship (IDALS) publication for Proper Management of Flooded Grain and Hay. That publication states “Farms disposing of spoiled grain and feed on their own property can land apply damaged grain at the following application rates: 146 bu corn/acre and 50 bu soybean ...


Anhydrous Ammonia Application -- Spring 2019, John E. Sawyer 2019 Iowa State University

Anhydrous Ammonia Application -- Spring 2019, John E. Sawyer

John E. Sawyer

It was a late harvest in fall 2018. Soils were wet and frozen when it was time to apply anhydrous ammonia. Those situations resulted in much less than normal anhydrous ammonia application last fall. Therefore, considerable anhydrous ammonia needs to be applied this spring. There is only so much capacity to switch from one nitrogen (N) fertilizer product to another. In Iowa, historically the two largest N fertilizers are anhydrous ammonia (largest) and urea-ammonium nitrate (UAN) solution, with granulated urea a distant third.


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