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Laboratory and Basic Science Research Commons

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Lps-Induced Inos Mrna And The Pro-Apoptotic Signaling Pathway In Leukocytes Of Fit And Unfit Males, Tiffany M. Zuniga 2018 Virginia Commonwealth University

Lps-Induced Inos Mrna And The Pro-Apoptotic Signaling Pathway In Leukocytes Of Fit And Unfit Males, Tiffany M. Zuniga

Theses and Dissertations

Overexpression of the enzyme iNOS induces apoptotic cellular death by increasing indices of pro-inflammation and oxidative stress. Aerobic physical activity has been known to have anti- inflammatory benefits and reduce oxidative stress. Purpose: Therefore, this study aimed to examine the impact of aerobic fitness on LPS-induced iNOS mRNA expression and the relationship of this expression with indices of oxidative stress, pro-inflammation and apoptosis in isolated leukocytes. Methods: Whole blood samples from aerobically fit and unfit males were stimulated with and without LPS. Thereafter, iNOS mRNA expression and MDA, TNF-α and p53 concentrations were analyzed. Results: iNOS mRNA expression levels following ...


Coloration, Camouflage, And Sexual Dichromatism In The Northern Pacific Rattlesnake (Crotalus Oreganus), Jefferson Brooks 2018 Central Washington University

Coloration, Camouflage, And Sexual Dichromatism In The Northern Pacific Rattlesnake (Crotalus Oreganus), Jefferson Brooks

All Master's Theses

In some viperid snakes, natural selection has shaped coloration and patterning to match local habitats, and for males to show greater warning coloration than females due to a less sedentary lifestyle. The Northern Pacific Rattlesnake (Crotalus oreganus) is likely the most abundant ambush predator in eastern Washington, yet little is known about its color variation, how color patterns interact with their habitats, and if male and female snakes show differences in patterning or color. To explore how snake color might interact with the colors of their environment, I used standardized digital photography to record both snake and substrate coloration. I ...


Relationship Between Perceived And Actual Exertion And Enjoyment Of Exercise In Individuals With Autism, Nicole Fiscella 2018 James Madison University

Relationship Between Perceived And Actual Exertion And Enjoyment Of Exercise In Individuals With Autism, Nicole Fiscella

Masters Theses

Fiscella, N.A., Moran, T.E., Wigglesworth, J.K., and McKay, C.A. Relationship between Perceived and Actual Exertion and Enjoyment of Exercise in Individuals with Autism. Purpose: The purpose of this study was to examine the relationship between perceived exertion, actual exertion and enjoyment of exercise in individuals with Autism Spectrum Disorder (ASD). Methods: A total of 16 participants (12 males and 4 females) between the ages of 5 and 38 who were diagnosed with ASD participated in the study. The intervention lasted 10 weeks and consisted of participation in one of James Madison University’s Overcoming Barriers hour ...


Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi 2018 University of Colorado, Boulder

Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi

Undergraduate Honors Theses

Heart disease continues to be the leading cause of death in both men and women. It is accompanied with cellular- and organ- level remodeling that cannot be mimicked or recaptured by cells on standard culture platforms. Polyethylene glycol hydrogels bridge this gap by providing user-defined tunability for cell culture. A novel 3D cell culture system was developed to encapsulate neonatal rat ventricular cardiomyocytes (NRVMs) in a single plane, and cell health was assessed. In parallel, recombinant adenoviruses containing the mutations responsible for human hypertrophic cardiomyopathy (HCM) in the beta myosin gene (R403Q and R453C) were generated and used as a ...


Morphological Comparison Of Fourth-Instar Larvae From Various Populations Of Wyeomyia Smithii, Kendra D. Moore 2018 Georgia Southern University

Morphological Comparison Of Fourth-Instar Larvae From Various Populations Of Wyeomyia Smithii, Kendra D. Moore

University Honors Program Theses

Populations of pitcher plant mosquitoes, Wyeomyia smithii, have exhibited recent shifts in blood-feeding behavior, likely in response to changes in climate and food availability. A comparison of various morphological structures in fourth-instar larvae was conducted to investigate the hypothesis that geographic and temporal dietary differences are reflected in changes in organismal size.


The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr 2018 University of Massachusetts Amherst

The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr

Doctoral Dissertations

Plant cell growth is a meticulously regulated process whereby the cell wall is selectively loosened to allow for turgor-pressure driven expansion. The rate of expansion must equal delivery of new material, or the cell will lyse. In many plant cells, this process happens diffusely around the cell. However, a number of plant cells have anisotropic shapes that require exquisite spatial control of secretion. One simple example of anisotropic patterning is tip growth; highly polarized cell expansion utilized by pollen tubes, root hairs, and moss protonemata. Investigating the role various molecules have in tip growth sheds light on how plant cells ...


Investigation Of Female Versus Male Firefighter Anthropometrics And Ergonomic Mobility, Meredith McQuerry 2018 Florida State University

Investigation Of Female Versus Male Firefighter Anthropometrics And Ergonomic Mobility, Meredith Mcquerry

International Textile and Apparel Association (ITAA) Annual Conference Proceedings

The purpose of this study was to assess the impact of fit on female versus male firefighter range of motion (ROM) when donning a structural turnout ensemble. Three test ensembles were evaluated: base layers (BL), turnout suit (TS), and turnout ensemble (TE). Sixteen career firefighters (10 males; 6 females) completed a user needs survey on fit and mobility restrictions, were measured in a three-dimensional body scanner, performed a static ROM protocol, and recorded ease of movement and comfort perceptions for each ensemble. Average body measurements and absolute ROM values were calculated for each test ensemble and analyzed by gender. Results ...


2d Pattern Development Of Cycle Wear From 3d Human Body Scan Data, HYUN JEONG PARK, WOLHEE DO 2018 Chonnam National University

2d Pattern Development Of Cycle Wear From 3d Human Body Scan Data, Hyun Jeong Park, Wolhee Do

International Textile and Apparel Association (ITAA) Annual Conference Proceedings

The purpose of this study is to develop cycling shorts considering the lower body surface changes in male high school cyclists according to their cycling postures. In this method, a male high school cyclist who wears "M" size was selected and the 3D human body scan was performed. In the case of cycling, the lower body posture was shown at various angles by the bending of the body and the rotation of the leg (Cha, 2013). Therefore, the postures of the waist and the knee were set to 145° and 90° in this study. The human body scan was performed ...


Lorcaserin As A Potential Opioid-Sparing Adjunct, Kumiko M. Lippold 2018 Virginia Commonwealth University

Lorcaserin As A Potential Opioid-Sparing Adjunct, Kumiko M. Lippold

Theses and Dissertations

Opioids, such as oxycodone, morphine, and fentanyl, are commonly used medications in the treatment of moderate to severe pain. In spite of their efficacious analgesic properties, their increased prescribing rates by physicians and inherent abuse-related effects have led to the ongoing opioid epidemic. Their clinical utility is limited by the risk of adverse dose-dependent side effects, such as constipation and respiratory depression, and the development of tolerance and dependence. Opioid-sparing adjunctive therapies are sought to address these issues by reducing the dose of opioid needed to achieve analgesia through alternative non-opioidergic mechanisms and as a result, reduce the incidence of ...


The Rhetoric Of Science Education And Technology, Iwasan D. Kejawa 2018 University of Nebraska-Lincoln

The Rhetoric Of Science Education And Technology, Iwasan D. Kejawa

CSE Journal Articles

Nearly thousands of science experiments are performed both on humans and animals every year in the United States (Gregory, 1999). Does Science enormously play a role in the well-beings of individual in the society? Research has found that science education is through motivation and satisfying the needs of humans. The scientific world is part of an elongated human development. This can be substantiated with the use and evolution of TECHNOLOGY and SCIENCE (Minton, 2004). Education of the entities that comprise the need to achieve the goal of TECHNOLOGY and SCIENCE which are important issues of today. Research has shown that ...


Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave 2018 Iowa State University

Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave

Education Publications

The popularity of TV shows such as Crime Scene Investigation (CSI) has generated high school students’ interest in forensics. Yet, forensic science is not commonly accessible to students, and especially students of color who often attend under-resourced high schools. This article presents the design, development, and evaluation of an online forensics course created for high school students of color who were a part of an informal science, technology, engineering, and mathematics (STEM) educational development program. Two essential elements guided the course design: the target learners (high school students of color) and integrating online instruction and hands-on laboratory activities involving real-world ...


Laboratory Focus On Improving The Culture Of Biosafety: Statewide Risk Assessment Of Clinical Laboratories That Process Specimens For Microbiologic Analysis, Erik Munson, Erin J. Bowles, Richard Dern, eric Beck, Raymond P. Podzorski, Allen C. Batemman, Timothy K. Block, Joshua L. Kropp, Tyler Radke, Karen Siebers, Brian Simmons, Mary A. Smith, Frances Spray-Larson, David M. Warshauer 2018 Marquette University

Laboratory Focus On Improving The Culture Of Biosafety: Statewide Risk Assessment Of Clinical Laboratories That Process Specimens For Microbiologic Analysis, Erik Munson, Erin J. Bowles, Richard Dern, Eric Beck, Raymond P. Podzorski, Allen C. Batemman, Timothy K. Block, Joshua L. Kropp, Tyler Radke, Karen Siebers, Brian Simmons, Mary A. Smith, Frances Spray-Larson, David M. Warshauer

Clinical Lab Sciences Faculty Research and Publications

The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source ...


A Retrospective Analysis Of Treatment And Clinical Outsomes Among Patients With Methicillin-Susceptible Staphlococcus Aureus Bloodstream Isolates Possessing Detecable Meca By A Commercial Pcr Assay Compared To Patients With Methicillin-Resistant Staphylococcus Aureus Bloodstream Isolates, Dylan Jones, Ramy Elshaboury, Erik Munson, Thomas J. Dilworth 2018 aAscension, Wheaton Franciscan-St. Francis Campus, Department of Pharmacy

A Retrospective Analysis Of Treatment And Clinical Outsomes Among Patients With Methicillin-Susceptible Staphlococcus Aureus Bloodstream Isolates Possessing Detecable Meca By A Commercial Pcr Assay Compared To Patients With Methicillin-Resistant Staphylococcus Aureus Bloodstream Isolates, Dylan Jones, Ramy Elshaboury, Erik Munson, Thomas J. Dilworth

Clinical Lab Sciences Faculty Research and Publications

mecA-positive Staphylococcus aureus isolates phenotypically susceptible to cefoxitin (mecA-methicillin-sensitive S. aureus[MSSA]) have been identified. We describe the treatment and outcomes among patients with mecA-MSSA bloodstream infections (BSI) and MRSA BSI matched 1:1 for age, BSI origin, and BSI type (n = 17 per group). Compared to MRSA BSI patients, mecA-MSSA BSI patients more often experienced clinical failure (58.8% and 11.8%, P = 0.010), driven largely by persistent bacteremia (35.3% and 11.8%). mecA-MSSA BSI patients may be at higher risk for poor clinical outcomes.


Dna Base Excision Repair And Double Strand Break Repair In Human Fibroblast, Mingyang Li 2017 Louisiana State University and Agricultural and Mechanical College

Dna Base Excision Repair And Double Strand Break Repair In Human Fibroblast, Mingyang Li

LSU Doctoral Dissertations

In eukaryotes, DNA repair mechanisms detect and repair damaged DNA. DNA damage is primarily caused by a variety of exogenous and endogenous sources. Several types of damage to DNA are repaired by different kinds of DNA repair pathways. This dissertation focused on repair of N-methylpurines (NMPs) and double-strand breaks (DSBs) in human fibroblasts.

NMPs, including N7-methylguanine (7MeG) and N3-methyladenine (3MeA), can be induced by environmental methylating agents (e.g. the soil fumigant methyl bromide), chemotherapeutics (e.g. nitrogen mustards), and natural cellular methyl donors like S-adenosylmethionine. In human cells, NMPs are repaired by the multi-step ...


Effect Of Metal Ion Substitution On Electrochemical Properties Of Cobalt Oxide For Energy Applications, Dalal Alqahtani 2017 Pittsburg State University

Effect Of Metal Ion Substitution On Electrochemical Properties Of Cobalt Oxide For Energy Applications, Dalal Alqahtani

Electronic Thesis Collection

Metal oxides are being used for various applications, such as batteries, supercapacitors, and catalysts. This research is mainly to produce high-performance energy storage devices capable of working at high temperatures and causing no pollution in the environment. To this end, the effect of metal ion substitution on the electrochemical properties of Co3O4 for energy applications was studied. Different metal oxides, such as FeCo2O4, MnCo2O4, Co3O4, NiCo2O4, CuCo2O4, and ZnCo2O4, were synthesized using a hydrothermal process. These metal oxides were ...


Aging, Inflammation, And Gut Microbiota In Mice, Jody Demeo 2017 Bowling Green State University

Aging, Inflammation, And Gut Microbiota In Mice, Jody Demeo

Honors Projects

Inflammation and gut microbiota are two areas of study that can be linked to aging in the body. As a person ages, systemic inflammation tends to increase, and changes in microbiota in the gastrointestinal system occur as well.


Detection Of Duo-Schistosome Infection From Filtered Urine Samples From School Children In Zambia After Mda, Megan J. Hessler, Austin Cyrs, Steven C. Krenzke, El Shaimaa Mahmoud, Chummy Sikasunge, James C. L. Mwansa, Nilanjan Lodh 2017 Marquette University

Detection Of Duo-Schistosome Infection From Filtered Urine Samples From School Children In Zambia After Mda, Megan J. Hessler, Austin Cyrs, Steven C. Krenzke, El Shaimaa Mahmoud, Chummy Sikasunge, James C. L. Mwansa, Nilanjan Lodh

Clinical Lab Sciences Faculty Research and Publications

Schistosomiasis is one of the major Neglected Tropical Diseases (NTDs) in sub-Saharan Africa. In sub-Saharan Africa, two major human schistosome species namely Schistosoma mansoniand S. haematobium often occur sympatrically largely affecting children. Recognizing the public health impact of Schistosomiasis, the World Health Organization (WHO) is urging member states to regularly treat at least 75% and up to 100%, of all school-aged children at risk of morbidity. For control strategies based on targeted mass drug administration (MDA) to succeed it is essential to have a simple and sensitive test for monitoring the success of these interventions. Current available diagnostic tests ...


Zinc Chloride Activates Phospho-Akt And Promotes Chondrocyte Maturation In The Atdc5 Chondrogenic Cell Line, Donya T. Burgess 2017 Seton Hall University

Zinc Chloride Activates Phospho-Akt And Promotes Chondrocyte Maturation In The Atdc5 Chondrogenic Cell Line, Donya T. Burgess

Seton Hall University Dissertations and Theses (ETDs)

Fracture healing is characterized by inflammation, cartilage formation, bone deposition, and remodeling. Endochondral ossification is an important part of callus formation that relies on the maturation of chondrocytes. Early in their maturation these cells express collagen type II alpha 1 chain (COL2A1) and express collagen type X alpha 1 chain (COL10A1) as they mature further and become hypertrophic chondrocytes. Activation of the insulin pathway is considered a primary factor involved in the initiation of chondrogenesis and its progression during bone healing. Insulin mimetics like zinc chloride (ZnCl2) have been shown to improve bone healing outcomes and enhance bone healing ...


Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson 2017 University of the Incarnate Word

Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson

Theses & Dissertations

Research Focus. Rheumatoid arthritis (RA) is a chronic, debilitating, autoimmune disease that causes the destruction of bone tissue and the articular structures of joints. At least 30% of RA patient populations have cognitive impairment. Acidic dopamine (DA) is the principal neuroimmunotransmitter that links the central nervous system and peripheral nervous system together. The aim of the present study was to determine the levels of DA and its two acidic metabolites: 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in arthritic induced rats, and whether their levels vary across four different parts of the brain: amygdala (AMG), front cerebral cortex (CX ...


Characterization Of Notch1 And Pi3k-Pten-Akt/Mtor Pathway Interaction In Head And Neck Squamous Cell Carcinoma, Kyriante' Henry 2017 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Characterization Of Notch1 And Pi3k-Pten-Akt/Mtor Pathway Interaction In Head And Neck Squamous Cell Carcinoma, Kyriante' Henry

UT GSBS Dissertations and Theses (Open Access)

Head and neck squamous cell carcinoma (HNSCC) affects various mucosal sites of the upper aerodigestive tract, including the nasal and oral cavities, the nasopharynx, and the oropharynx. More than five hundred thousand new cases of HNSCC occurred in 2011 alone, with 50,000 reported cases in the United States. This trend made HNSCC the seventh most common non-skin cancer worldwide (Ferlay et al., 2015). Although significant epidemiological and pathological advancements have been made, survival rates have not improved much over the last 40 years, leaving a mortality rate that remains at approximately 50%. An unbiased drug screen demonstrated that HNSCC ...


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