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Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati 2010 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India

Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati

Rajesh Pati

Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15oC. Somatic ...


The Effect Of T Cell Receptor Specificity On Cd4+Cd25+ Regulatory T Cell Function In An Autoimmune Setting, Soyoung Oh 2010 University of Pennsylvania

The Effect Of T Cell Receptor Specificity On Cd4+Cd25+ Regulatory T Cell Function In An Autoimmune Setting, Soyoung Oh

Publicly Accessible Penn Dissertations

The studies presented in this dissertation examine how the T cell receptor (TCR) specificity of CD4+CD25+ regulatory T (Treg) cells affects their function in a mouse model of autoimmune arthritis. TS1xHACII mice co-express CD4+ T cells that express the transgenic 6.5 TCR, which is specific for the S1 determinant of influenza virus PR8 hemagglutinin (HA), and HA as a neo-self antigen under the MHC Class II I-Eα promoter. The majority of TS1xHACII mice develop inflammatory arthritis that is driven by recognition of S1 peptide by 6.5+CD4+ T cells. Notably, arthritis develops despite the presence of CD4 ...


Understanding Acquired Resistance To Lapatinib In Breast Cancer Cells, Jen-Te Tseng 2010 University of Texas Graduate School of Biomedical Sciences at Houston

Understanding Acquired Resistance To Lapatinib In Breast Cancer Cells, Jen-Te Tseng

UT GSBS Dissertations and Theses (Open Access)

Signaling through epidermal growth factor receptor (EGFR/ErbB) family members plays a very important role in regulating proliferation, development, and malignant transformation of mammary epithelial cells. ErbB family members are often over-expressed in human breast carcinomas. Lapatinib is an ErbB1 and ErbB2 tyrosine kinase inhibitor that has been shown to have anti-proliferative effects in breast and lung cancer cells. Cells treated with Lapatinib undergo G1 phase arrest, followed by apoptosis. Lapatinib has been approved for clinical use, though patients have developed resistance to the drug, as seen previously with other EGFR inhibitors. Moreover, the therapeutic efficacy varies significantly within the ...


Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability, Diem T. Kha 2010 University of Texas Graduate School of Biomedical Sciences at Houston

Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability, Diem T. Kha

UT GSBS Dissertations and Theses (Open Access)

Genetic instability in mammalian cells can occur by many different mechanisms. In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a Z-DNA or H-DNA, this can lead to genetic instability in the form of DNA double-strand breaks (DSBs) (1, 2). Our laboratory found that the stability of these non-B DNA structures was different in mammals versus Escherichia coli (E.coli) bacteria (1, 2). One explanation for the difference between these species may be a ...


Modeling Sporadic Tumor Formation Driven By Telomere Dysfunction In The Gastrointestinal Tract, Suzanne S. Chan 2010 University of Texas Graduate School of Biomedical Sciences at Houston

Modeling Sporadic Tumor Formation Driven By Telomere Dysfunction In The Gastrointestinal Tract, Suzanne S. Chan

UT GSBS Dissertations and Theses (Open Access)

Colorectal cancer is a complex disease that is thought to arise when cells accumulate mutations that allow for uncontrolled growth. There are several recognized mechanisms for generating such mutations in sporadic colon cancer; one of which is chromosomal instability (CIN). One hypothesized driver of CIN in cancer is the improper repair of dysfunctional telomeres. Telomeres comprise the linear ends of chromosomes and play a dual role in cancer. Its length is maintained by the ribonucleoprotein, telomerase, which is not a normally expressed in somatic cells and as cells divide, telomeres continuously shorten. Critically shortened telomeres are considered dysfunctional as they ...


A Metagenomic Study Of The Tick Midgut, Daniel T. Yuan 2010 University of Texas Graduate School of Biomedical Sciences at Houston

A Metagenomic Study Of The Tick Midgut, Daniel T. Yuan

UT GSBS Dissertations and Theses (Open Access)

A Metagenomic Study of the Tick Midgut
Daniel Yuan, B.S.
Supervisory Professor : Steven J. Norris, Ph.D.
Southern tick–associated rash illness (STARI) or Master’s disease is a Lyme-like illness that occurs following bites by Amblyomma americanum, the lone-star tick. Clinical symptoms include a bull’s eye rash similar to the erythema migrans lesions of Lyme disease, as well as fever and joint pains. Lyme disease is caused by Borrelia burgdorferi and related spirochetes. However, B. burgdorferi has not been detected in STARI patients, or in ticks in the South Central U.S. The causative agent of STARI ...


New Target Genes For Tumor Suppressors P53 And P73 In Regenerating Liver, Svitlana M. Kurinna 2010 University of Texas Graduate School of Biomedical Sciences at Houston

New Target Genes For Tumor Suppressors P53 And P73 In Regenerating Liver, Svitlana M. Kurinna

UT GSBS Dissertations and Theses (Open Access)

The p53-family of proteins regulates expression of target genes during tissue development and differentiation. Within the p53-family, p53 and p73 have hepatic-specific functions in development and tumor suppression. Despite a growing list of p53/p73 target genes, very few of these have been studied in vivo, and the knowledge regarding functions of p53 and p73 in normal tissues remains limited. p53+/-p73+/- mice develop hepatocellular carcinoma (HCC), whereas overexpression of p53 in human HCC leads to tumor regression. However, the mechanism of p53/p73 function in liver remains poorly characterized. Here, the model of mouse liver regeneration is used to ...


Cross-Sector Review Of Drivers And Available 3rs Approaches For Acute Systemic Toxicity Testing, Troy Seidle, Sally Robinson, Tom Holmes, Stuart Creton, Pilar Prieto, Julia Scheel, Magda Chlebus 2010 Humane Society International

Cross-Sector Review Of Drivers And Available 3rs Approaches For Acute Systemic Toxicity Testing, Troy Seidle, Sally Robinson, Tom Holmes, Stuart Creton, Pilar Prieto, Julia Scheel, Magda Chlebus

Experimentation Collection

Acute systemic toxicity studies are carried out in many sectors in which synthetic chemicals are manufactured or used and are among the most criticized of all toxicology tests on both scientific and ethical grounds. A review of the drivers for acute toxicity testing within the pharmaceutical industry led to a paradigm shift whereby in vivo acute toxicity data are no longer routinely required in advance of human clinical trials. Based on this experience, the following review was undertaken to identify (1) regulatory and scientific drivers for acute toxicity testing in other industrial sectors, (2) activities aimed at replacing, reducing, or ...


Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati 2010 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India

Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati

Rajesh Pati

No abstract provided.


Wku Scholar, Pamela Napier, editor-in-chief 2010 Western Kentucky University

Wku Scholar, Pamela Napier, Editor-In-Chief

WKU Scholar

No abstract provided.


Toxicity Testing In The 21st Century: A Vision And A Strategy, Daniel Krewski, Daniel Acosta Jr, Melvin Anderson, Henry Anderson, John C. Bailar III, Kim Boekelheide, Robert Brent, Gail Charnley, Vivian G. Cheung, Sidney Green Jr, Karl T. Kelsey, Nancy I. Kerkvliet, Abby A. Li, Lawrence McCray, Otto Meyer, Reid D. Patterson, William Pennie, Robert A. Scala, Gina M. Solomon, Martin Stephens, James Yager, Lauren Zeise 2010 University of Ottawa

Toxicity Testing In The 21st Century: A Vision And A Strategy, Daniel Krewski, Daniel Acosta Jr, Melvin Anderson, Henry Anderson, John C. Bailar Iii, Kim Boekelheide, Robert Brent, Gail Charnley, Vivian G. Cheung, Sidney Green Jr, Karl T. Kelsey, Nancy I. Kerkvliet, Abby A. Li, Lawrence Mccray, Otto Meyer, Reid D. Patterson, William Pennie, Robert A. Scala, Gina M. Solomon, Martin Stephens, James Yager, Lauren Zeise

Experimentation Collection

With the release of the landmark report Toxicity Testing in the 21st Century: A Vision and a Strategy, the U.S. National Academy of Sciences, in 2007, precipitated a major change in the way toxicity testing is conducted. It envisions increased efficiency in toxicity testing and decreased animal usage by transitioning from current expensive and lengthy in vivo testing with qualitative endpoints to in vitro toxicity pathway assays on human cells or cell lines using robotic high-throughput screening with mechanistic quantitative parameters. Risk assessment in the exposed human population would focus on avoiding significant perturbations in these toxicity pathways. Computational ...


Pseudo-Merohedral Twinning And Noncrystallographic Symmetry In Orthorhombic Crystals Of Sivmac239 Nef Core Domain Bound To Different-Length Tcrzeta Fragments., Walter Minsub Kim, Alexander B. Sigalov, Lawrence J. Stern 2010 University of Massachusetts Medical School

Pseudo-Merohedral Twinning And Noncrystallographic Symmetry In Orthorhombic Crystals Of Sivmac239 Nef Core Domain Bound To Different-Length Tcrzeta Fragments., Walter Minsub Kim, Alexander B. Sigalov, Lawrence J. Stern

MD/PhD Program

HIV/SIV Nef mediates many cellular processes through interactions with various cytoplasmic and membrane-associated host proteins, including the signalling zeta subunit of the T-cell receptor (TCRzeta). Here, the crystallization strategy, methods and refinement procedures used to solve the structures of the core domain of the SIVmac239 isolate of Nef (Nef(core)) in complex with two different TCRzeta fragments are described. The structure of SIVmac239 Nef(core) bound to the longer TCRzeta polypeptide (Leu51-Asp93) was determined to 3.7 A resolution (R(work) = 28.7%) in the tetragonal space group P4(3)2(1)2. The structure of SIVmac239 Nef(core ...


Southern Lagoon And Village Of Gales Point, Belize, Ca: Water Uses, Water Quality, And Potential Health Impacts, Rasmi Nair, Ritchie Taylor 2010 Western Kentucky University

Southern Lagoon And Village Of Gales Point, Belize, Ca: Water Uses, Water Quality, And Potential Health Impacts, Rasmi Nair, Ritchie Taylor

Impact Belize

An International Health Service Learning Program, Impact Belize 2010, was conducted by Western Kentucky University. Faculty and students worked closely with Gales Point villagers to gain an insight into the use of Southern Lagoon. The objective of the study was to assess fecal coliform levels in Southern lagoon, its primary and secondary contact use by villagers and its potential impact on health. Study methodology involved collection of water samples, both near shore and offshore, in the lagoon and incubating them using ColiQuant EZ method approved by EPA. A questionnaire was administered to villagers attending the WKU clinic to collect data ...


Building Better Scientists Through Cross-Disciplinary Collaboration In Synthetic Biology: A Report From The Genome Consortium For Active Teaching Workshop 2010, Michael J. Wolyniak, Consuelo J. Alvarez, Vidya Chandrasekaran, Theresa M. Grana, Andrea Holgado, Christopher J. Jones, Robert W. Morris, Anil L. Pereira, Joyce Stamm, Talitha M. Washington, Yixin Yang 2010 University of Mary Washington

Building Better Scientists Through Cross-Disciplinary Collaboration In Synthetic Biology: A Report From The Genome Consortium For Active Teaching Workshop 2010, Michael J. Wolyniak, Consuelo J. Alvarez, Vidya Chandrasekaran, Theresa M. Grana, Andrea Holgado, Christopher J. Jones, Robert W. Morris, Anil L. Pereira, Joyce Stamm, Talitha M. Washington, Yixin Yang

Biological Sciences Research

A common problem faced by primarily undergraduate institutions is the lack of funding and material support needed to adequately expose students to modern biology, including synthetic biology. To help alleviate this problem, the Genome Consortium for Active Teaching (GCAT) was founded in 2000 by Malcolm Campbell at Davidson College to bring genomics into the undergraduate curriculum. GCAT’s first tangible activity was to serve as a central clearinghouse both for the purchase and reading of DNA microarrays and for information on how to execute genomics experiments at undergraduate institutions. In response to the evolution of molecular biology in the last ...


The Current Scientific And Legal Status Of Alternative Methods To The Ld50 Test For Botulinum Neurotoxin Potency Testing, Sarah Adler, Gerd Bicker, Hans Bigalke, Christopher Bishop, Jörg Blümel, Dirk Dressler, Joan Fitzgerald, Frank Gessler, Heide Heuschen, Birgit Kegel, Andreas Luch, Catherine Milne, Andrew Pickett, Heidemarie Ratsch, Irmela Ruhdel, Dorothea Sesardic, Martin Stephens, Gerhard Stiens, Peter D. Thornton, René Thürmer, Martin Vey, Horst Spielmann, Barbara Grune, Manfred Liebsch 2010 Centre for Documentation and Evaluation of Alternatives to Animal Experiments (ZEBET)

The Current Scientific And Legal Status Of Alternative Methods To The Ld50 Test For Botulinum Neurotoxin Potency Testing, Sarah Adler, Gerd Bicker, Hans Bigalke, Christopher Bishop, Jörg Blümel, Dirk Dressler, Joan Fitzgerald, Frank Gessler, Heide Heuschen, Birgit Kegel, Andreas Luch, Catherine Milne, Andrew Pickett, Heidemarie Ratsch, Irmela Ruhdel, Dorothea Sesardic, Martin Stephens, Gerhard Stiens, Peter D. Thornton, René Thürmer, Martin Vey, Horst Spielmann, Barbara Grune, Manfred Liebsch

Experimentation Collection

No abstract provided.


Free-Living Inferential Modeling Of Blood Glucose Level Using Only Noninvasive Inputs, Derrick K. Rollins, Nidhi Bhandari, Jim Kleinedler, Kaylee Kotz, Amber Strohbehn, Lindsay Boland, Megan Murphy, Dave Andre, Nisarg Vyas, Greg Welk, Warren D. Franke 2010 Iowa State University

Free-Living Inferential Modeling Of Blood Glucose Level Using Only Noninvasive Inputs, Derrick K. Rollins, Nidhi Bhandari, Jim Kleinedler, Kaylee Kotz, Amber Strohbehn, Lindsay Boland, Megan Murphy, Dave Andre, Nisarg Vyas, Greg Welk, Warren D. Franke

Warren D Franke

The goal of this work is to present a causation modeling methodology with the ability to accurately infer blood glucose levels using a large set of highly correlated noninvasive input variables over an extended period of time. These models can provide insight to improve glucose monitoring, and glucose regulation through advanced model-based control technologies. The efficacy of this approach is demonstrated using real data from a type 2 diabetic (T2D) subject collected under free-living conditions over a period of 25 consecutive days. The model was identified and tested using eleven variables that included three food variables as well as several ...


Regulation Of Crbp1 In Mammary Epithelial Cells, Stacy L. Pease 2010 University of Massachusetts Amherst

Regulation Of Crbp1 In Mammary Epithelial Cells, Stacy L. Pease

Masters Theses 1911 - February 2014

Breast cancer is the second leading cause of death of women in the United States, warranting further investigation into preventative therapies. It has been well documented that early pregnancy results in a lifetime decreased risk of breast cancer in humans and mounting evidence suggests that the retinoic acid pathway may play an important role in this protective effect. Cellular retinol binding protein-1 (CRBP1) is an essential component of the retinoic acid pathway and we propose that it plays an important role in pregnancy-induced protection against breast cancer. In order to investigate the role of CRBP1 in parity-induced protection against breast ...


Observational Learning In Wild And Captive Dolphins, Deirdre Yeater, Stan A. Kuczaj II 2010 Sacred Heart University

Observational Learning In Wild And Captive Dolphins, Deirdre Yeater, Stan A. Kuczaj Ii

Psychology Faculty Publications

Many non-human species imitate the behavior of others, and dolphins seem particularly adept at this form of observational learning. Evidence for observational learning in wild dolphins is rare, given the difficulty of observing individual wild animals in sufficient detail to eliminate other possible explanations of purported imitation. Consequently, much of the evidence supporting observational learning in dolphins has involved animals in captive settings. This research suggests that dolphins have an affinity for mimicry, and that they are more successful at observational learning if they choose to imitate another rather than being asked to do so. These results, combined with those ...


Environmental Monitoring: Acoustic Wireless Sensors For Pest Detection, Adam Osseiran 2010 Edith Cowan University

Environmental Monitoring: Acoustic Wireless Sensors For Pest Detection, Adam Osseiran

ECU Research Week

Presented at a Showcase of Research at the School of Engineering.


Application Of Systematic Review Methodology To Food And Feed Safety Assessments To Support Decision Making, Jonathan J. Deeks, Geoff K. Frampton, Julie M. Glanville, Mattias Greiner, Julian P. T. Higgins, Gabor Lövei, Annette M. O'Connor, Andrew S. Pullin, Andrijana Rajić 2010 University of Birmingham

Application Of Systematic Review Methodology To Food And Feed Safety Assessments To Support Decision Making, Jonathan J. Deeks, Geoff K. Frampton, Julie M. Glanville, Mattias Greiner, Julian P. T. Higgins, Gabor Lövei, Annette M. O'Connor, Andrew S. Pullin, Andrijana Rajić

Veterinary Diagnostic and Production Animal Medicine Publications

Systematic reviews are commonly used in human health research to provide overviews of existing evidence pertinent to clearly formulated specific questions, using pre-specified and standardised methods to identify and critically appraise relevant research, and to collect, report and analyse data from the studies that are included in the reviews. Formal systematic reviews have rarely been used in food and feed safety risk assessments and the existing systematic review methods in other disciplines may not be directly applicable in this field. This Guidance aims to assist the application of systematic reviews to food and feed safety risk assessments in support of ...


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