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Laboratory and Basic Science Research Commons

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Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave 2019 Iowa State University

Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave

Constance P. Hargrave

The popularity of TV shows such as Crime Scene Investigation (CSI) has generated high school students’ interest in forensics. Yet, forensic science is not commonly accessible to students, and especially students of color who often attend under-resourced high schools. This article presents the design, development, and evaluation of an online forensics course created for high school students of color who were a part of an informal science, technology, engineering, and mathematics (STEM) educational development program. Two essential elements guided the course design: the target learners (high school students of color) and integrating online instruction and hands-on laboratory activities involving real-world ...


Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal 2019 Clark Atlanta University

Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

In this study, we investigated differential activation and the role of two mTOR complexes in cell migration of prostate cancer cells. Specific knock-down of endogenous RAPTOR and RICTOR by siRNA resulted in decreased cell migration in LNCaP, DU145, and PC3 cells indicating that both mTORC1 and mTORC2 are required for cell migration. EGF treatment induced the activation of both mTORC1 and mTORC2 as determined by complex-specific phosphorylation of mTOR protein. Specific knock-down or inhibition of Rac1 activity in PC3 cells blocked EGF-induced activation of mTORC2, but had no effect on mTORC1 activation. Furthermore, the over-expression of constitutively active Rac1 (Rac1Q61L ...


Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore 2019 Atlanta University Center

Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

The largest U.S. cancer health disparity exists in prostate cancer, with African American men having the highest incidence and mortality rates. The present study evaluated the effects of ZIC2 and the underlying mechanisms in the E006 parental African-American cell line that produces tumors at accelerated growth rates because of the increase of ZIC2 genes in African-American males. We analyzed the experimental research that the overexpression of ZIC2 contributes to progression of prostate cancer. E006AA cells with overexpressed or suppressed ZIC2 were analyzed to determine phenotypic differences, PCR, cell proliferation and immunoblot assays. The expression levels of ZIC2 were analyzed ...


Jet Lag: What’S Light Got To Do With It?, Melissa Richardson 2019 Andrews University

Jet Lag: What’S Light Got To Do With It?, Melissa Richardson

Adventist Human-Subject Researchers Association

Jet lag results in memory loss and sleepiness. Current treatments for jet lag focus on symptoms and not the cause of jet lag; disrupted light environment. This study in mice, uncovered mechanisms of how light regulates jet lag recovery and memory. This work informs our future studies in humans.


A Discrepancy Between The Human Reference Genome (Grch37) And Transcriptome (Refseq) Results In The Incorrect Annotation Of A Clinically-Relevant Sequence Variant In Recql4, Lisa A. Lansdon 2019 Children's Mercy Kansas City

A Discrepancy Between The Human Reference Genome (Grch37) And Transcriptome (Refseq) Results In The Incorrect Annotation Of A Clinically-Relevant Sequence Variant In Recql4, Lisa A. Lansdon

Research Days

No abstract provided.


Forward Osmosis Flow Rate Differential Using Different Osmotic Agents, John M. Trzinski 2019 Embry Riddle Aeronautical University

Forward Osmosis Flow Rate Differential Using Different Osmotic Agents, John M. Trzinski

Beyond: Undergraduate Research Journal

Life support is one of the most vital systems flown on manned spaceflight missions. The systems currently used are large, heavy, inefficient, and power consuming in an environment that requires nearly perfect conditions to function. Forward Osmosis (FO) is a form of filtration that uses the natural properties of water and concentration gradients to filter water without the need for any electricity. An experiment was conducted during the Embry Riddle Summer 2018 study abroad to the LUNARES research station in Piła, Poland. This experiment explored how the flow rate of water through a FO filtration system could be affected by ...


Identification Of Anaerobes In Clinical Specimens Comparison Between The Rapid Ana Ii System And The Bruker Maldi-Tof Ms System Utilized In The Clinical Laboratory, Raymond Chow 2019 Dominican University of California

Identification Of Anaerobes In Clinical Specimens Comparison Between The Rapid Ana Ii System And The Bruker Maldi-Tof Ms System Utilized In The Clinical Laboratory, Raymond Chow

Natural Sciences and Mathematics | Clinical Laboratory Sciences Culminating Projects

Matrix-Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been revealed as an invaluable platform for identifying anaerobic bacteria in the clinical laboratory over traditional methods such as the RapID ANA II System.

A qualitative comparison is made, through the analysis of methodologies and specifications, to determine whether the RapID ANA II system or Bruker MALDI-TOF MS is more suitable for identifying anaerobic organism in the clinical laboratory. Based on the data reviewed, the MALDI-TOF MS is a more intuitive platform within the clinical laboratory due to its increased specificity, cost-effectiveness, and shorten turnaround time for the identification ...


Detection Of Genetic Thrombophilia (Fa V Leiden & Pt20210 Mutation) Using Genexpert Technology With Iqcp Implementation, Mariegrace Saquilayan 2019 Dominican University of California

Detection Of Genetic Thrombophilia (Fa V Leiden & Pt20210 Mutation) Using Genexpert Technology With Iqcp Implementation, Mariegrace Saquilayan

Natural Sciences and Mathematics | Clinical Laboratory Sciences Culminating Projects

Abstract

Individuals having Genetic Thrombophilia pose a higher risk of having a thrombotic event. It is crucial to determine these gene variants to prevent a possible episode of thromboembolism. With the current PCR method, it involves individual processing of DNA isolation, amplification, and detection using three (3) different instruments resulting to an increased turnaround time of 5 to 7 days and additional staff utilization. It is performed by repetitive manual sample pipetting and preparation of reagent master mixes in small vials. Results interpretations are entered manually to a worklist built initially for final verification. These processes increase the risk of ...


Acute Promyelocytic Leukemia: A Case Study Correlating Cytogenetic Abnormality With Prognosis, Alyssa Lamond, Theresa Tellier-Castellone 2019 University of Rhode Island

Acute Promyelocytic Leukemia: A Case Study Correlating Cytogenetic Abnormality With Prognosis, Alyssa Lamond, Theresa Tellier-Castellone

Senior Honors Projects

Advancements in medical technology today have positively impacted the diagnosis, treatment, and prognosis of cancers. Particularly acute promyelocytic leukemia (APL) has completely improved from having the poorest prognosis to one of the best. Acute promyelocytic leukemia is a malignant disease of hematopoietic tissue classified by WHO as leukemia with >20% blasts from the myeloid lineage, specifically promyelocytes. Determined in 1976, FAB classified AML subtypes M1-M7, with APL being M3. Specific characteristics classify the subtype of AML, with each resulting from a different genetic abnormality. The focus of APL diagnosis, treatment, and prognosis occurs around the known PML-RARα fusion gene. Flow ...


Thiol-Based Misfolding: Linking Redox Balance To Cytosolic Proteostasis, Ford Amy 2019 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Thiol-Based Misfolding: Linking Redox Balance To Cytosolic Proteostasis, Ford Amy

UT GSBS Dissertations and Theses (Open Access)

The eukaryotic cytosolic proteome is vulnerable to changes in proteostatic and redox balance caused by temperature, pH, oxidants and xenobiotics. Cysteine-containing proteins are especially at risk as the thiol side chain is subject to oxidation, adduction and chelation by thiol-reactive compounds. All of these thiol-modifiers have been demonstrated to induce the heat shock response and recruit protein chaperones to sites of presumed protein aggregation in the budding yeast Saccharomyces cerevisiae. However, endogenous targets of thiol stress toxicity responsible for these outcomes are largely unknown. Furthermore, I hypothesize proteins identified as redox-active are prone to misfolding and aggregation by thiol-specific stress ...


Seasonal Changes In Susceptibility To Chytridiomycosis In Columbia Spotted Frogs, Delaney Burrows 2019 Carroll College

Seasonal Changes In Susceptibility To Chytridiomycosis In Columbia Spotted Frogs, Delaney Burrows

Carroll College Student Undergraduate Research Festival

Chytridiomycosis is a potentially deadly skin disease found in amphibian populations all over the world. Chytridiomycosis is caused by the fungus Batrachochytrium dendrobatidis (Bd) that grows on the skin of amphibians. The fungus can cause a disruption in the homeostatic functions of the amphibian skin and can lead to death. The relationship between an amphibian’s antimicrobial skin secretions, cutaneous microbial communities and environmental water quality can affect their resistance and susceptibility to Bd infection. Columbia spotted frogs (Lithobates luteiventris) are native to western Montana and have been affected by chytridiomycosis. Three lakes known to be positive for Bd were ...


Potential Antimicrobial Activity Of Plants In The Genus Plantago, McKenna Hunt 2019 University of North Alabama

Potential Antimicrobial Activity Of Plants In The Genus Plantago, Mckenna Hunt

Scholars Week 2019

The aim of this study was to investigate potential antimicrobial activity of phytochemical extracts of commercially available species of Plantago. Plantago is a medically relevant genus, with several species traditionally used worldwide to treat inflammation and various infections. Dried leaf material of both species was extracted using a Soxhlet apparatus. Nonpolar (hexane) extracts were obtained from both P. subnuda and P. eriopoda, as well as a polar (methanol) extract from P. eriopoda. Minimum inhibitory concentration (MIC) assays were performed using 96-well microtiter plates against seven different bacterial species (Escherichia coli, Bacillus megaterium, Klebsiella pneumoniae, Mycobacterium smegmatis, Pseudomonas aeruginosa, Serratia marcescens ...


2018 Guide Summer Research - Breast Cancer, Qur'Annah Jones, DeLawnia Comer-Hagans 2019 Governors State University

2018 Guide Summer Research - Breast Cancer, Qur'annah Jones, Delawnia Comer-Hagans

GSU Research Day

This research poster details the relationship between cancer treatments and alternative approaches, such as mindfulness. The purpose of this study was to determine the methodology used by doctors to treat breast cancer and their willingness to try a holistic approach. This study was conducted on site at the UIC Cancer Center as well as remotely within hospitals around the Chicagoland area. We determined that mindfulness is in fact a worthwhile asset to breast cancer patients and that the field of oncology could support further research.


Publication Reform To Safeguard Wildlife From Researcher Harm, Kate A. Field, Paul C. Paquet, Kyle A. Artelle, Gilbert Proulx, Ryan K. Brook, Chris T. Darimont 2019 University of Victoria

Publication Reform To Safeguard Wildlife From Researcher Harm, Kate A. Field, Paul C. Paquet, Kyle A. Artelle, Gilbert Proulx, Ryan K. Brook, Chris T. Darimont

Professional Science Research Ethics Collection

Despite abundant focus on responsible care of laboratory animals, we argue that inattention to the maltreatment of wildlife constitutes an ethical blind spot in contemporary animal research. We begin by reviewing significant shortcomings in legal and institutional oversight, arguing for the relatively rapid and transformational potential of editorial oversight at journals in preventing harm to vertebrates studied in the field and outside the direct supervision of institutions. Straightforward changes to animal care policies in journals, which our analysis of 206 journals suggests are either absent (34%), weak, incoherent, or neglected by researchers, could provide a practical, effective, and rapidly imposed ...


Rgd-Decorated Cholesterol Stabilized Polyplexes For Targeted Sirna Delivery To Glioblastoma Cells., Bo Lou, Kate Connor, Kieron Sweeney, Ian S. Miller, Alice C. O'Farrell, Eduardo Ruiz-Hernandez, David M. Murray, Garry P. Duffy, Alan Wolfe, Enrico Mastrobattista, Annette Byrne, Wim E. Hennink 2019 Utrecht University

Rgd-Decorated Cholesterol Stabilized Polyplexes For Targeted Sirna Delivery To Glioblastoma Cells., Bo Lou, Kate Connor, Kieron Sweeney, Ian S. Miller, Alice C. O'Farrell, Eduardo Ruiz-Hernandez, David M. Murray, Garry P. Duffy, Alan Wolfe, Enrico Mastrobattista, Annette Byrne, Wim E. Hennink

Physiology and Medical Physics Articles

The development of an effective and safe treatment for glioblastoma (GBM) represents a significant challenge in oncology today. Downregulation of key mediators of cell signal transduction by RNA interference is considered a promising treatment strategy but requires efficient, intracellular delivery of siRNA into GBM tumor cells. Here, we describe novel polymeric siRNA nanocarriers functionalized with cRGD peptide that mediates targeted and efficient reporter gene silencing in U87R invasive human GBM cells. The polymer was synthesized via RAFT copolymerization of N-(2-hydroxypropyl)-methacrylamide (HPMA) and N-acryloxysuccinimide (NAS), followed by post-polymerization modification with cholesterol for stabilization, cationic amines for siRNA complexation, and ...


The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli 2019 The University of Southern Mississippi

The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli

Master's Theses

The spotted seatrout (Cynoscion nebulosus) is a euryhaline finfish candidate for marine aquaculture in the northern Gulf of Mexico. Aquaculture of this species currently uses salinities of 25 psu or greater making production challenging in areas where high salinity water is not available. This study aimed to document the effects of salinity on zootechnical performance of spotted seatrout embryos, early larvae, and juveniles in recirculating aquaculture systems to assist with the development of protocols for low salinity culture.

Embryos obtained from a volitional spawn at 29 psu were incubated at three salinities (12.5, 18.75, or 25 psu) until ...


Characterizing Aft1/2-Grx3/4 Interaction And The Role Of Bol2 During Iron Regulation In Saccharomyces Cerevisiae, William Rivers 2019 University of South Carolina - Columbia

Characterizing Aft1/2-Grx3/4 Interaction And The Role Of Bol2 During Iron Regulation In Saccharomyces Cerevisiae, William Rivers

Senior Theses

Iron dysregulation has been linked to a variety of human diseases, such as anemia, Friedreich’s ataxia, X-linked sideroblastic anemia, sideroblastic-like microcytic anemia, and myopathy. Thus, it is vitally important to understand the mechanisms for regulating intracellular iron. Here, we use fluorescence microscopy techniques in live cells to study interactions of the yeast proteins Grx3/4, Aft1/2, and Bol2, which have been shown to be involved in turning off iron import when the cell has adequate iron. Modified versions of genes encoding these proteins have been incorporated into several yeast backgrounds to use fluorescence to monitor interactions under varying ...


An Automated Bayesian Pipeline For Rapid Analysis Of Single-Molecule Binding Data, Carlas Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grünwald 2019 University of Massachusetts Medical School

An Automated Bayesian Pipeline For Rapid Analysis Of Single-Molecule Binding Data, Carlas Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grünwald

David Grünwald

Single-molecule binding assays enable the study of how molecular machines assemble and function. Current algorithms can identify and locate individual molecules, but require tedious manual validation of each spot. Moreover, no solution for high-throughput analysis of single-molecule binding data exists. Here, we describe an automated pipeline to analyze single-molecule data over a wide range of experimental conditions. In addition, our method enables state estimation on multivariate Gaussian signals. We validate our approach using simulated data, and benchmark the pipeline by measuring the binding properties of the well-studied, DNA-guided DNA endonuclease, TtAgo, an Argonaute protein from the Eubacterium Thermus thermophilus. We ...


Day To Day And Leg To Leg Variation In Gene Expression, Zohal Alizai 2019 University of Nebraska at Omaha

Day To Day And Leg To Leg Variation In Gene Expression, Zohal Alizai

Student Research and Creative Activity Fair

BACKGROUND: Skeletal muscle biopsies are extensively used in research to determine the effects of exercise. Usually, sequential biopsies are taken before and after exercise in order to determine the influence of exercise on gene expression. Often it is not clear if the results are due to exercise or as a result of damage cause by the biopsy to the muscle. Previous studies have proven that multiple biopsies in the same leg cause stress to the muscle and such stress leads to inflammation and other response pathways that alter the gene expression. PURPOSE: The purpose of this study is to determine ...


Andrews University Receives Naacls Accreditation, Hannah Gallant 2019 Andrews University

Andrews University Receives Naacls Accreditation, Hannah Gallant

Andrews Agenda: Campus News

No abstract provided.


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