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Influence Of Functionalized Fullerene Structure On Polymer Photovoltaic Degradation, Brian H. Johnson, Enaanake Allagoa, Robert L. Thomas, Gregory Stettler, Marianne Wallis, Justn H. Peel, Thorsteinn Adalsteinsson, Brian J. McNelis, Richard P. Barber Jr. 2010 Santa Clara University

Influence Of Functionalized Fullerene Structure On Polymer Photovoltaic Degradation, Brian H. Johnson, Enaanake Allagoa, Robert L. Thomas, Gregory Stettler, Marianne Wallis, Justn H. Peel, Thorsteinn Adalsteinsson, Brian J. Mcnelis, Richard P. Barber Jr.

Physics

The time dependence of device performance has been measured for photocells using blends containing the conjugated polymer, poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV) with two different functionalized C60 electron acceptor molecules: commercially available [6,6]-phenyl C61 butyric acid methyl ester (PCBM) or [6,6]-phenyl C61 butyric acid octadecyl ester (PCBOD) produced in this laboratory. Performance was characterized by the short-circuit current output of the devices, with the time dependence of PCBM samples typically degrading exponentially. Variations in the characteristic lifetime of the devices were observed to depend on the molar fraction of the electron acceptor molecules (calculated with ...


Reverse Genetic And Cell Biological Approaches To The Study Of Developmental Functions Of Class Xi Myosin In Arabidopsis Thaliana, Eunsook Park 2010 University of Tennessee - Knoxville

Reverse Genetic And Cell Biological Approaches To The Study Of Developmental Functions Of Class Xi Myosin In Arabidopsis Thaliana, Eunsook Park

Doctoral Dissertations

Myosin proteins function as molecular motors that drive the ATP-dependent movement of cellular components along actin filaments. Vascular plants encode two different types of myosin, referred to as class VIII and class XI. Although class XI myosins have been suggested to function in organelle movement and cytoplasmic streaming, little is known about their cellular function in detail.

The Arabidopsis genome encodes 13 class XI myosin genes. The reasons for the relatively large number of myosin XI isoforms present within a single plant species are unknown. To investigate the function of these gene products in the cell, we determined the spatial ...


Maternal Consumption Of Canola Oil Suppressed Mammary Gland Tumorigenesis In C3(1) Tag Mice Offspring, Gabriela Ion, Juliana A. Akinsete, W. Elaine Hardman 2010 Marshall University

Maternal Consumption Of Canola Oil Suppressed Mammary Gland Tumorigenesis In C3(1) Tag Mice Offspring, Gabriela Ion, Juliana A. Akinsete, W. Elaine Hardman

Biochemistry and Microbiology

Background: Maternal consumption of a diet high in omega 6 polyunsaturated fats (n-6 PUFA) has been shown to increase risk whereas a diet high in omega 3 polyunsaturated fats (n-3 PUFA) from fish oil has been shown to decrease risk for mammary gland cancer in female offspring of rats. The aim of this study was to determine whether increasing n-3 PUFA and reducing n-6 PUFA by using canola oil instead of corn oil in the maternal diet might reduce the risk for breast cancer in female offspring.

Methods: Female SV 129 mice were divided into two groups and placed on ...


Development And Validation Of A Rapid Method For The Detection Of Latrunculol A In Plasma., Jiajiu Shaw, Frederick A. Valeriote, Joseph Media, Tyler A. Johnson, Taro Amagata, Karen Tenney, Phillip Crews 2010 Henry Ford Health System, Detroit, Michigan, USA

Development And Validation Of A Rapid Method For The Detection Of Latrunculol A In Plasma., Jiajiu Shaw, Frederick A. Valeriote, Joseph Media, Tyler A. Johnson, Taro Amagata, Karen Tenney, Phillip Crews

Natural Sciences and Mathematics | Faculty Scholarship

Latrunculol A is a recently discovered 6,7-dihydroxy analog of the potent actin inhibitor latrunculin A. Latrunculol A has exhibited greater cytotoxicity than latrunculin A against both murine and human colon tumor cell lines in vitro. Currently, there are no reports regarding the bioavailability of latrunculol A in vivo. This study was undertaken as a prelude to pharmacokinetic assessments and it is the first work where bioavailability of latrunculol A was studied. In the present work, a simple plasma preparation and a rapid HPLC method have been developed. Mouse plasma containing latrunculol A was first treated by acetonitrile and then ...


C-Jun Nh2-Terminal Kinase Is Required For Lineage-Specific Differentiation But Not Stem Cell Self-Renewal, Ping Xu, Roger J. Davis 2010 University of Massachusetts Medical School

C-Jun Nh2-Terminal Kinase Is Required For Lineage-Specific Differentiation But Not Stem Cell Self-Renewal, Ping Xu, Roger J. Davis

Davis Lab Publications

The c-Jun NH(2)-terminal kinase (JNK) is implicated in proliferation. Mice with a deficiency of either the Jnk1 or the Jnk2 genes are viable, but a compound deficiency of both Jnk1 and Jnk2 causes early embryonic lethality. Studies using conditional gene ablation and chemical genetic approaches demonstrate that the combined loss of JNK1 and JNK2 protein kinase function results in rapid senescence. To test whether this role of JNK was required for stem cell proliferation, we isolated embryonic stem (ES) cells from wild-type and JNK-deficient mice. We found that Jnk1(-/-) Jnk2(-/-) ES cells underwent self-renewal, but these cells proliferated ...


Quasispecies-Like Behavior Observed In Catalytic Rna Populations Evolving In A Test Tube, Carolina Diaz Arenas, Niles Lehman 2010 Portland State University

Quasispecies-Like Behavior Observed In Catalytic Rna Populations Evolving In A Test Tube, Carolina Diaz Arenas, Niles Lehman

Chemistry Faculty Publications and Presentations

Background: During the RNA World, molecular populations were probably very small and highly susceptible to the force of strong random drift. In conjunction with Muller's Ratchet, this would have imposed difficulties for the preservation of the genetic information and the survival of the populations. Mechanisms that allowed these nascent populations to overcome this problem must have been advantageous.

Results: Using continuous in vitro evolution experimentation with an increased mutation rate imposed by MnCl2, it was found that clonal 100-molecule populations of ribozymes clearly exhibit certain characteristics of a quasispecies. This is the first time this has been seen with ...


Crystallization Of Community-Acquired Respiratory Distress Syndrome Toxin From Mycoplasma Pneumonia, Olga N. Pakhomova, Alexander B. Taylor, Argentina Becker, Stephen P. Holloway, T. R. Kannan, Joel B. Baseman, P. John Hart 2010 Old Dominion University

Crystallization Of Community-Acquired Respiratory Distress Syndrome Toxin From Mycoplasma Pneumonia, Olga N. Pakhomova, Alexander B. Taylor, Argentina Becker, Stephen P. Holloway, T. R. Kannan, Joel B. Baseman, P. John Hart

Bioelectrics Publications

Community-acquired respiratory distress syndrome toxin (CARDS TX) is a 591-amino-acid protein with ADP-ribosyltransferase and vacuolating activities that damages the cells lining the respiratory tracts of patients infected with the bacterial pathogen Mycoplasma pneumoniae. Crystals of CARDS TX were grown in space group C2, with unit-cell parameters a = 191.4, b = 107.4, c = 222.1 A, beta = 90.6 degrees. A complete 2.2 A data set was obtained from a single CARDS TX crystal.


T Cell Specific Adapter Protein (Tsad) Interacts With Tec Kinase Itk To Promote Cxcl12 Induced Migration Of Human And Murine T Cells, Tone Berge, Vibeke Sundvold-Gjerstad, Stine Granum, Thorny Cecilie Bie Andersen, Gunn B. Holthe, Lena Claesson-Welsh, Amy H. Andreotti, Marit Inngjerdingen, Anne Spurkland 2010 University of Oslo

T Cell Specific Adapter Protein (Tsad) Interacts With Tec Kinase Itk To Promote Cxcl12 Induced Migration Of Human And Murine T Cells, Tone Berge, Vibeke Sundvold-Gjerstad, Stine Granum, Thorny Cecilie Bie Andersen, Gunn B. Holthe, Lena Claesson-Welsh, Amy H. Andreotti, Marit Inngjerdingen, Anne Spurkland

Biochemistry, Biophysics and Molecular Biology Publications

The chemokine CXCL12/SDF-1α interacts with its G-protein coupled receptor CXCR4 to induce migration of lymphoid and endothelial cells. T cell specific adapter protein (TSAd) has been found to promote migration of Jurkat T cells through interaction with the G protein β subunit. However, the molecular mechanisms for how TSAd influences cellular migration have not been characterized in detail. We show that TSAd is required for tyrosine phosphorylation of the Lck substrate IL2-inducible T cell kinase (Itk). Presence of Itk Y511 was necessary to boost TSAd's effect on CXCL12 induced migration of Jurkat T cells. In addition, TSAd's ...


Site-Directed Mutagenesis And Pban Activation Of The Helicoverpa Zea Pban-Receptor, Man-Yeon Choi, Russell A. Jurenka 2010 United States Department of Agriculture

Site-Directed Mutagenesis And Pban Activation Of The Helicoverpa Zea Pban-Receptor, Man-Yeon Choi, Russell A. Jurenka

Entomology Publications

Pheromone biosynthesis-activating neuropeptide (PBAN) and pyrokinins belong to a family of insect peptide hormones that have a common FXPRLamide C-terminal ending. The G-protein-coupled receptors (GPCRs) for this peptide family were first identified from a moth and Drosophila with sequence similarity to neuromedin U receptors from vertebrates. We have characterized the PBAN-receptor (PBAN-R or PR) active binding domains using chimeric GPCRs and proposed that extracellular loop 3 is critical for ligand selection. Here, we characterized the 3rd extracellular domain of PBAN-R through site-directed point mutations. Results are discussed in context of the structural features required for receptor activation using receptor activation ...


The Saccharomyces Sun Gene, Uth1, Is Involved In Cell Wall Biogenesis, Father Nicanor Austriaco 2010 Providence College

The Saccharomyces Sun Gene, Uth1, Is Involved In Cell Wall Biogenesis, Father Nicanor Austriaco

Rev. Nicanor Austriaco, O.P.

Deletion of the Saccharomyces gene, UTH1, a founding member of the SUN family of fungal genes, has pleiotropic effects. Several phenotypes of Δuth1 cells including their decreased levels of mitochondrial proteins, their impaired autophagic degradation of mitochondria, and their increased viability in the presence of mammalian BAX, a proapoptotic regulator localized to the mitochondria, have prompted others to propose that the Uth1p functions primarily at the mitochondria. In this report, we show that cells lacking UTH1 have more robust cell walls with higher levels of β-d-glucan that allows them to grow in the presence of calcofluor white or sodium dodecyl ...


Requirements For Transitional Endoplasmic Reticulum Site Structure And Function In Saccharomyces Cerevisiae, Polina Shindiapina, Charles Barlowe 2010 Dartmouth College

Requirements For Transitional Endoplasmic Reticulum Site Structure And Function In Saccharomyces Cerevisiae, Polina Shindiapina, Charles Barlowe

Open Dartmouth: Faculty Open Access Scholarship

Secretory proteins are exported from the endoplasmic reticulum (ER) at specialized regions known as the transitional ER (tER). Coat protein complex II (COPII) proteins are enriched at tER sites, although the mechanisms underlying tER site assembly and maintenance are not understood. Here, we investigated the dynamic properties of tER sites in Saccharomyces cerevisiae and probed protein and lipid requirements for tER site structure and function. Thermosensitive sec12 and sec16 mutations caused a collapse of tER sites in a manner that depended on nascent secretory cargo. Continual fatty acid synthesis was required for ER export and for normal tER site structure ...


Cell-Type Specific Distribution Of Chloride Transporters In The Rat Suprachiasmatic Nucleus, Michael A. Belenky, Patricia J. Sollars, David B. Mount, Seth L. Alper, Yosef Yarom, Gary E. Pickard 2010 Hebrew University of Jerusalem

Cell-Type Specific Distribution Of Chloride Transporters In The Rat Suprachiasmatic Nucleus, Michael A. Belenky, Patricia J. Sollars, David B. Mount, Seth L. Alper, Yosef Yarom, Gary E. Pickard

Papers in Veterinary and Biomedical Science

The suprachiasmatic nucleus (SCN) is a circadian oscillator and biological clock. Cell-to-cell communication is important for synchronization among SCN neuronal oscillators and the great majority of SCN neurons use γ-aminobutyric acid (GABA) as a neurotransmitter, the principal inhibitory neurotransmitter in the adult central nervous system. Acting via the ionotropic GABAA receptor, a chloride ion channel, GABA typically evokes inhibitory responses in neurons via Cl− influx. Within the SCN GABA evokes both inhibitory and excitatory responses although the mechanism underlying GABA-evoked excitation in the SCN is unknown. GABA-evoked depolarization in immature neurons in several regions of the brain is a function ...


Role Of Jak/Stat Pathway In The Pathogenesis Of Breast Cancer, qifang zhang 2010 Virginia Commonwealth University

Role Of Jak/Stat Pathway In The Pathogenesis Of Breast Cancer, Qifang Zhang

Theses and Dissertations

The Jak/Stat signaling cascade mediates cell proliferation, differentiation, survival, apoptosis and immune responses. Aberrant activation of this pathway mediates neoplastic transformation and abnormal growth of many malignancies including breast cancer, the most common cancer among women, and the second leading cause of cancer deaths in women in United States. The mechanism by which the Jak/Stat pathway modulates the pathogenesis of breast cancer is unclear. This dissertation elucidates roles of Jak/Stat members that mediate the pathogenesis of breast cancer. For these studies, we used 4T1 mouse mammary tumor cells as a model which mimics human breast cancer. First ...


Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi L. Reynolds, Pamela Sheaff 2010 University of Nebraska - Lincoln

Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi L. Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.


Fast Protein Structure Alignment, Yosi Shibberu, Allen Holder, Kyla Lutz 2010 Rose-Hulman Institute of Technology

Fast Protein Structure Alignment, Yosi Shibberu, Allen Holder, Kyla Lutz

Mathematical Sciences Technical Reports (MSTR)

We address the problem of aligning the 3D structures of two proteins. Our pairwise comparisons are based on a new optimization model that is succinctly expressed in terms of linear transformations and highlights the problem’s intrinsic geometry. The optimization problem is approximately solved with a new polynomial time algorithm. The worst case analysis of the algorithm shows that the solution is bounded by a constant depending only on the data of the problem.


Replication And Active Demethylation Represent Partially Overlapping Mechanisms For Erasure Of H3k4me3 In Budding Yeast, Marta Radman-Livaja, Chih Long Liu, Nir Friedman, Stuart L. Schreiber, Oliver J. Rando 2010 University of Massachusetts Medical School

Replication And Active Demethylation Represent Partially Overlapping Mechanisms For Erasure Of H3k4me3 In Budding Yeast, Marta Radman-Livaja, Chih Long Liu, Nir Friedman, Stuart L. Schreiber, Oliver J. Rando

Open Access Articles

Histone modifications affect DNA-templated processes ranging from transcription to genomic replication. In this study, we examine the cell cycle dynamics of the trimethylated form of histone H3 lysine 4 (H3K4me3), a mark of active chromatin that is viewed as "long-lived" and that is involved in memory during cell state inheritance in metazoans. We synchronized yeast using two different protocols, then followed H3K4me3 patterns as yeast passed through subsequent cell cycles. While most H3K4me3 patterns were conserved from one generation to the next, we found that methylation patterns induced by alpha factor or high temperature were erased within one cell cycle ...


The Heat Capacity And Thermodynamic Properties Of The Iron Oxides And Their Relation To The Mineral Core Of The Iron Storage Protein Ferritin, Claine Lindsey Morton Snow 2010 Brigham Young University - Provo

The Heat Capacity And Thermodynamic Properties Of The Iron Oxides And Their Relation To The Mineral Core Of The Iron Storage Protein Ferritin, Claine Lindsey Morton Snow

Theses and Dissertations

The iron oxides are a group of materials with geological, biological, and technological importance. A thermodynamic understanding of these materials is important because it provides information about their relative stabilities, chemical reactivity, and transformations. This study provides the heat capacity of a nanocrystalline magnetite (Fe3O4) sample, bulk hematite (α-Fe2O3), nanocrystalline hematite, akaganéite (β-FeOOH), and lepidocrocite (γ-FeOOH) at temperatures as low as 0.5 K. These measurements were fit to theoretical functions at temperatures lower than 15 K, and the respective thermophysical properties of these materials are discussed. Also the molar entropies of bulk hematite and hydrous nanocrystalline hematite as well ...


Microtensile Bond Strength Of Self-Adhesive Resin-Based Dental Cement, SELDA KESKIN 2010 The Pennsilyvania State University, Department of Chemistry

Microtensile Bond Strength Of Self-Adhesive Resin-Based Dental Cement, Selda Keskin


This study investigated the effectiveness of bleaching agents on the micro tensile bond strength (mTBS) of self-adhesive luting cement and enamel. A total of 126 samples were prepared from the labial surfaces of permanent human maxillary central incisors and assigned into three groups with 42 samples each as: control, enamel bleached with 10% hydrogen peroxide (HP), and enamel bleached with 10% carbamide peroxide (CP). Rely X Unicem1 (3 M, ESPE) was used as self-adhesive resin cement. The total testing period for bleaching was selected as 14 days and the bleaching agents were applied 8 hours a day. The statistical one-way ...


A Natural Missing Link Between Activated And Downhill Protein Folding Scenarios, Feng Liu, Caroline Maynard, Gregory E. Scott, Artem Melnykov, Kathleen B. Hall, Martin Gruebele 2010 University of Illinois at Urbana-Champaign

A Natural Missing Link Between Activated And Downhill Protein Folding Scenarios, Feng Liu, Caroline Maynard, Gregory E. Scott, Artem Melnykov, Kathleen B. Hall, Martin Gruebele

Chemistry and Biochemistry

We propose protein PTB1:4W as a good candidate for engineering into a downhill folder. PTB1:4W has a probe-dependent thermal unfolding curve and sub-millisecond T-jump relaxation kinetics on more than one time scale. Its refolding rate in denaturant is a non-linear function of denaturant concentration (curved chevron plot). Yet at high denaturant concentration its unfolding is probe-independent, and the folding kinetics can be fitted to a single exponential decay. The domain appears to fold via a mechanism between downhill folding and activated folding over several small barriers, and when denaturant is added, one of these barriers greatly increases and ...


Abundance Of Ammonia-Oxidizing Archaea And Bacteria Along An Estuarine Salinity Gradient In Relationship To Potential Nitrification Rates, Anne E. Bernhard, Zachary C. Landry, Alison Blevins, José R. de la Torre, Anne E. Giblin, David A. Stahl 2010 Connecticut College

Abundance Of Ammonia-Oxidizing Archaea And Bacteria Along An Estuarine Salinity Gradient In Relationship To Potential Nitrification Rates, Anne E. Bernhard, Zachary C. Landry, Alison Blevins, José R. De La Torre, Anne E. Giblin, David A. Stahl

Biology Faculty Publications

Abundance of ammonia-oxidizing Archaea (AOA) was found to be always greater than that of ammonia-oxidizing Bacteria along an estuarine salinity gradient, and AOA abundance was highest at intermediate salinity. However, AOA abundance did not correlate with potential nitrification rates. This lack of correlation may be due to methodological limitations or alternative energy sources.


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