Open Access. Powered by Scholars. Published by Universities.®

Medicinal-Pharmaceutical Chemistry Commons

Open Access. Powered by Scholars. Published by Universities.®

Analytical chemistry

Articles 1 - 3 of 3

Full-Text Articles in Medicinal-Pharmaceutical Chemistry

Enantiomeric Chromatographic Separations And Ionic Liquids , Jie Ding Jan 2005

Enantiomeric Chromatographic Separations And Ionic Liquids , Jie Ding

Retrospective Theses and Dissertations

The chiral nature of compounds contributes to their bioactivity and their various pharmaceutical/industrial uses. As a result, the Food and Drug Administration (FDA) has implemented policies for analyzing the enantiomers of chiral compounds. Gas chromatography (GC) and liquid chromatography (LC) utilizing chiral stationary phases are the two most commonly used methods to achieve direct enantiomeric separations of chiral compounds. The first part of this dissertation includes a review of direct separation of enantiomers in gas and liquid chromatography, an example of the enantiomeric separation of various racemic sulfoxides and sulfinate esters on four derivatized cyclodextrin chiral stationary phases using ...


Capillary Electrophoresis Separation Of Neutral Organic Compounds, Pharmaceutical Drugs, Proteins And Peptides, Enantiomers And Anions , Wei-Liang Ding Jan 1998

Capillary Electrophoresis Separation Of Neutral Organic Compounds, Pharmaceutical Drugs, Proteins And Peptides, Enantiomers And Anions , Wei-Liang Ding

Retrospective Theses and Dissertations

Addition of a novel anionic surfactant, namely lauryl polyoxyethylene sulfate, to an aqueous-acetonitrile electrolyte makes it possible to separate nonionic organic compounds by capillary electrophoresis. Separation is based on differences in the association between analytes and the surfactant. Highly hydrophobic compounds such as polyaromatic hydrocarbons are well separated by this new surfactant. Migration times of analytes can be readily changed over an unusually large range by varying the additive concentration and the proportion of acetonitrile in the electrolyte. Several examples are given, including the separation of four methylbenz (a) anthracene isomers and the separation of normal and deuterated acetophenone;The ...


Combination Of Capillary Isoelectric Focusing And Liquid Chromatography With Electrospray Ionization Mass Spectrometry For Protein Characterization , Jing Wei Jan 1998

Combination Of Capillary Isoelectric Focusing And Liquid Chromatography With Electrospray Ionization Mass Spectrometry For Protein Characterization , Jing Wei

Retrospective Theses and Dissertations

Combination of capillary isoelectric focusing (CIEF) and liquid chromatography (LC) with electrospray ionization mass spectrometry (ESIMS) is utilized for protein characterization;Both CIEF-ESIMS and LC-MS are studied for phosphoprotein characterization. At the intact protein level, on-line CTEF-ESIMS is applied for phosphorylation analysis to obtain the two dimensional information: isoelectric point and molecular mass. At the peptide level, on-line LC-ESI-MS and LC-ESI neutral loss MS/MS were highly specific for the determination of sites of phosphorylation and successfully applied on the tryptic digest of tau proteins phosphorylated by different kinases;On-line combination of CIEF with ESI-TOF MS is demonstrated for high ...