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Medicinal-Pharmaceutical Chemistry Commons

Open Access. Powered by Scholars. Published by Universities.®

2010

Chemically-modified RNA

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Nmr Resonance Assignment Of The Therapeutic Rna Aptamer Macugen In Complex With Its In Vivo Target, The Heparin Binding Domain Of Vegf165, Andrew Eric Libby Jan 2010

Nmr Resonance Assignment Of The Therapeutic Rna Aptamer Macugen In Complex With Its In Vivo Target, The Heparin Binding Domain Of Vegf165, Andrew Eric Libby

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

Aptamers consist of nucleic acids that are selected from an in vitro library in order to bind a specific target or catalyze a reaction. Many aptamer-ligand structures have been solved in recent years to characterize the molecular mechanisms that explain the high affinity and specificity observed in these interactions. To this end, NMR resonance assignment experiments were performed as a necessary first step to calculating the solution structure of a therapeutically-active RNA aptamer, Macugen, bound to its in vivo target, the heparin binding domain (HBD) of vascular endothelial growth factor 165 (VEGF165). Though the resonance assignment of Macugen is challenging ...