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A Prostaglandin Synthase Inhibition Assay With Detection By Elisa, Patricia Scott
Theses, Dissertations & Honors Papers
A simple, reliable method is described for the routine measurement of non-steroidal anti-inflammatory drugs (NSAIDs) as inhibitors of prostaglandin synthase. Appropriate concentrations of inhibitor are incubated with ram seminal vesical prostaglandin synthase, sodium arachidonate and reduced glutathione. Prostaglandin E2 (PGE2) production is then quantitated by and enzyme linked immunosorbant assay (ELISA), in which anti-PGE2 antibody is utilized in a binding competition between test sample and adsorbed conjugate of PGE2-BSA. The antibody which remains bound to the solid phase is quantitatively determined colorimetrically by incubation with horse radish peroxidase labeled goat anti-rabbit IgG followed by incubation ...