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Cytoskeleton

Genetics

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Full-Text Articles in Cell Biology

Reduced Tubulin Polyglutamylation Suppresses Flagellar Shortness In Chlamydomonas, Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, Ritsu Kamiya, George B. Witman Aug 2015

Reduced Tubulin Polyglutamylation Suppresses Flagellar Shortness In Chlamydomonas, Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, Ritsu Kamiya, George B. Witman

Cell and Developmental Biology Publications

Ciliary length control is an incompletely understood process essential for normal ciliary function. The flagella of Chlamydomonas mutants lacking multiple axonemal dyneins are shorter than normal; previously it was shown that this shortness can be suppressed by the mutation suppressor of shortness 1 (ssh1) via an unknown mechanism. To elucidate this mechanism, we carried out genetic analysis of ssh1 and found that it is a new allele of TPG2 (hereafter tpg2-3), which encodes FAP234 functioning in tubulin polyglutamylation in the axoneme. Similar to the polyglutamylation-deficient mutants tpg1 and tpg2-1, tpg2-3 axonemal tubulin has a greatly reduced level of long polyglutamate ...


Drc3 Connects The N-Drc To Dynein G To Regulate Flagellar Waveform, Junya Awata, Kangkang Song, Jianfeng Lin, Stephen M. King, Michael J. Sanderson, Daniela Nicastro, George B. Witman Aug 2015

Drc3 Connects The N-Drc To Dynein G To Regulate Flagellar Waveform, Junya Awata, Kangkang Song, Jianfeng Lin, Stephen M. King, Michael J. Sanderson, Daniela Nicastro, George B. Witman

Cell and Developmental Biology Publications

The nexin-dynein regulatory complex (N-DRC), which is a major hub for the control of flagellar motility, contains at least 11 different subunits. A major challenge is to determine the location and function of each of these subunits within the N-DRC. We characterized a Chlamydomonas mutant defective in the N-DRC subunit DRC3. Of the known N-DRC subunits, the drc3 mutant is missing only DRC3. Like other N-DRC mutants, the drc3 mutant has a defect in flagellar motility. However, in contrast to other mutations affecting the N-DRC, drc3 does not suppress flagellar paralysis caused by loss of radial spokes. Cryo-electron tomography revealed ...