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Chromatin

Departmental Papers (Biology)

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Full-Text Articles in Cell Biology

Lamin B1 Depletion In Senescent Cells Triggers Large-Scale Changes In Gene Expression And The Chromatin Landscape, Parisha P. Shah, Greg Donahue, Gabriel Otte, Brian C. Capell, David M. Nelson, Kajia Cao, Varun Aggarwala, Hazel A. Cruickshanks, Taranjit Singh Rai, Tony Mcbryan, Brian D. Gregory, Peter D. Adams, Shelley L. Berger Aug 2013

Lamin B1 Depletion In Senescent Cells Triggers Large-Scale Changes In Gene Expression And The Chromatin Landscape, Parisha P. Shah, Greg Donahue, Gabriel Otte, Brian C. Capell, David M. Nelson, Kajia Cao, Varun Aggarwala, Hazel A. Cruickshanks, Taranjit Singh Rai, Tony Mcbryan, Brian D. Gregory, Peter D. Adams, Shelley L. Berger

Departmental Papers (Biology)

Senescence is a stable proliferation arrest, associated with an altered secretory pathway, thought to promote tumor suppression and tissue aging. While chromatin regulation and lamin B1 down-regulation have been implicated as senescence effectors, functional interactions between them are poorly understood. We compared genome-wide Lys4 trimethylation on histone H3 (H3K4me3) and H3K27me3 distributions between proliferating and senescent human cells and found dramatic differences in senescence, including large-scale domains of H3K4me3- and H3K27me3-enriched “mesas” and H3K27me3-depleted “canyons.” Mesas form at lamin B1-associated domains (LADs) in replicative senescence and oncogene-induced senescence and overlap DNA hypomethylation regions in cancer, suggesting that pre-malignant senescent chromatin ...