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Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

2014

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Articles 361 - 364 of 364

Full-Text Articles in Cell Biology

Structural Basis For Phosphorylation And Lysine Acetylation Cross-Talk In A Kinase Motif Associated With Myocardial Ischemia And Cardioprotection, Benjamin L. Parker, Nicholas E. Shepherd, Sophie Trefely, Nolan John Hoffman, Melanie Y. White, Kasper Engholm-Keller, Brett D. Hambly, Martin R. Larsen, David E. James, Stuart J. Cordwell Jan 2014

Structural Basis For Phosphorylation And Lysine Acetylation Cross-Talk In A Kinase Motif Associated With Myocardial Ischemia And Cardioprotection, Benjamin L. Parker, Nicholas E. Shepherd, Sophie Trefely, Nolan John Hoffman, Melanie Y. White, Kasper Engholm-Keller, Brett D. Hambly, Martin R. Larsen, David E. James, Stuart J. Cordwell

Faculty of Health Sciences Publications

Myocardial ischemia and cardioprotection by ischemic pre-conditioning induce signal networks aimed at survival or cell death if the ischemic period is prolonged. These pathways are mediated by protein post-translational modifications that are hypothesized to cross-talk with and regulate each other. Phosphopeptides and lysine-acetylated peptides were quantified in isolated rat hearts subjected to ischemia or ischemic pre-conditioning, with and without splitomicin inhibition of lysine deacetylation. We show lysine acetylation (acetyl-Lys)-dependent activation of AMP-activated protein kinase, AKT, and PKA kinases during ischemia. Phosphorylation and acetyl-Lys sites mapped onto tertiary structures were proximal in >50% of proteins investigated, yet they were mutually ...


Analysis Of The Role Of Two Autophagy Pathway Related Genes, Becn1 And Tsc1, In Murine Mammary Gland Development And Differentiation, Amber N. Hale Jan 2014

Analysis Of The Role Of Two Autophagy Pathway Related Genes, Becn1 And Tsc1, In Murine Mammary Gland Development And Differentiation, Amber N. Hale

Theses and Dissertations--Biology

The mammary gland is a dynamic organ that undergoes the majority of its development in the postnatal period in four stages; mature virgin, pregnancy, lactation, and involution. Every stage relies on tightly regulated cellular proliferation, programmed cell death, and tissue remodeling mechanisms. Misregulation of autophagy, an intracellular catabolic process to maintain energy stores, has long been associated with mammary tumorigenesis and other pathologies. We hypothesize that appropriate regulation and execution of autophagy are necessary for proper development of the mammary ductal tree and maintenance of the secretory epithelia during late pregnancy and lactation. To test this hypothesis we examined the ...


The Interactions Between Jak/Stat Signaling Ligands In Drosophila Melanogaster, Qian Chen Jan 2014

The Interactions Between Jak/Stat Signaling Ligands In Drosophila Melanogaster, Qian Chen

Theses and Dissertations--Biology

The development of multi-cellular organisms requires extensive cell-cell communication to coordinate cell functions. However, only a handful of signaling pathways have emerged to mediate all the intercellular communications; therefore, each of them is under an array of regulations to achieve signaling specificity and diversity. One such signaling pathway is the Janus Kinase/ Signal Transducer and Activator of Transcription (JAK/STAT) pathway, which is the primary signaling cascade responding to a variety of cytokines and growth factors in mammals and involved in many developmental processes. This signaling pathway is highly conserved between mammals and Drosophila ...


Requirements Of Rab5 Activity In Highly Invasive Breast Cancer Cell Lines, Nicole Porther, M Alejandro Barbieri Dec 2013

Requirements Of Rab5 Activity In Highly Invasive Breast Cancer Cell Lines, Nicole Porther, M Alejandro Barbieri

Nicole Porther

Rab5 expression in cancer has been associated with the disease progression and prognosis. We have previously shown that growth factor-directed cell invasion and migration was dependent on Rab5 activation in non-invasive breast cancer cells. However, hardly any data is available regarding the role of Rab5 in invasive cells in the presence of growth factor.  In our present study, we report that the invasive and migratory properties of the highly invasive breast cancer cell line, MDAMB-231, were abrogated in cells expressing the inactive (GDP-bound) form of Rab5 irrespective of growth factor stimulation; while the invasive potential of breast cancer cell lines ...