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Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

2010

Iowa State University

Veterinary Medicine

Articles 1 - 1 of 1

Full-Text Articles in Cell Biology

Detection Of Flaviviruses And Orthobunyaviruses In Mosquitoes In The Yucatan Peninsula Of Mexico In 2008, Jose A. Farfan-Ale, Maria A. Loroño-Pino, Julian E. Garcia-Rejon, Victor Soto, Ming Lin, Molly Staley, Karin S. Dorman, Lyric C. Bartholomay, Einat Hovav, Bradley J. Blitvich Oct 2010

Detection Of Flaviviruses And Orthobunyaviruses In Mosquitoes In The Yucatan Peninsula Of Mexico In 2008, Jose A. Farfan-Ale, Maria A. Loroño-Pino, Julian E. Garcia-Rejon, Victor Soto, Ming Lin, Molly Staley, Karin S. Dorman, Lyric C. Bartholomay, Einat Hovav, Bradley J. Blitvich

Entomology Publications

A total of 191,244 mosquitoes from 24 species were collected in the Yucatan Peninsula of Mexico from January to December 2008, and tested for the presence of cytopathic virus by virus isolation in Vero cells. Eighteen virus isolates were obtained, all of which were orthobunyaviruses. These were identified by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing as Cache Valley virus (n = 17) and South River virus (n = 1). A subset (n = 20,124) of Culex quinquefasciatus collected throughout the year was further tested by RT-PCR using flavivirus-specific primers. Flavivirus RNA was present in this mosquito species year-round. The ...