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Full-Text Articles in Cell Biology

Detection Of Flaviviruses And Orthobunyaviruses In Mosquitoes In The Yucatan Peninsula Of Mexico In 2008, Jose A. Farfan-Ale, Maria A. Loroño-Pino, Julian E. Garcia-Rejon, Victor Soto, Ming Lin, Molly Staley, Karin S. Dorman, Lyric C. Bartholomay, Einat Hovav, Bradley J. Blitvich Oct 2010

Detection Of Flaviviruses And Orthobunyaviruses In Mosquitoes In The Yucatan Peninsula Of Mexico In 2008, Jose A. Farfan-Ale, Maria A. Loroño-Pino, Julian E. Garcia-Rejon, Victor Soto, Ming Lin, Molly Staley, Karin S. Dorman, Lyric C. Bartholomay, Einat Hovav, Bradley J. Blitvich

Entomology Publications

A total of 191,244 mosquitoes from 24 species were collected in the Yucatan Peninsula of Mexico from January to December 2008, and tested for the presence of cytopathic virus by virus isolation in Vero cells. Eighteen virus isolates were obtained, all of which were orthobunyaviruses. These were identified by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing as Cache Valley virus (n = 17) and South River virus (n = 1). A subset (n = 20,124) of Culex quinquefasciatus collected throughout the year was further tested by RT-PCR using flavivirus-specific primers. Flavivirus RNA was present in this mosquito species year-round. The ...


Echinacea-Induced Cytosolic Ca2+ Elevation In Hek293, Lankun Wu, Eric W. Rowe, Ksenija Jeftinija, Srdija Jeftinija, Ludmila Rizhsky, Basil Nikolau, Jodi Mckay, Marian L. Kohut, Eve Wurtele Jan 2010

Echinacea-Induced Cytosolic Ca2+ Elevation In Hek293, Lankun Wu, Eric W. Rowe, Ksenija Jeftinija, Srdija Jeftinija, Ludmila Rizhsky, Basil Nikolau, Jodi Mckay, Marian L. Kohut, Eve Wurtele

Biochemistry, Biophysics and Molecular Biology Publications

Background: With a traditional medical use for treatment of various ailments, herbal preparations of Echinacea are now popularly used to improve immune responses. One likely mode of action is that alkamides from Echinacea bind to cannabinoid type 2 (CB2) receptors and induce a transient increase in intracellular Ca2+. Here, we show that unidentified compounds from Echinacea purpurea induce cytosolic Ca2+ elevation in non-immune-related cells, which lack CB2 receptors and that the Ca2+ elevation is not influenced by alkamides. Methods: A non-immune human cell line, HEK293, was chosen to evaluate E. purpurea root extracts and constituents as potential regulators of intracellular ...


Tor Is A Negative Regulator Of Autophagy In Arabidopsis Thaliana, Yimo Liu, Diane C. Bassham Jan 2010

Tor Is A Negative Regulator Of Autophagy In Arabidopsis Thaliana, Yimo Liu, Diane C. Bassham

Genetics, Development and Cell Biology Publications

Background: Autophagy is a protein degradation process by which cells recycle cytoplasmic contents under stress conditions or during senescence; a basal level of housekeeping autophagy also occurs under non-stressed conditions. Although a number of genes that function in autophagy (ATG genes) have been identified in plants, the upstream components that regulate the plant autophagy pathway are still obscure. Target of rapamycin (TOR) is a negative regulator of autophagy in both yeast and animals, and homologs of TOR in plants control plant growth and protein synthesis. However, a role for TOR in regulation of autophagy in plants has not been demonstrated ...


Moesin1 And Ve-Cadherin Are Required In Endothelial Cells During In Vivo Tubulogenesis, Ying Wang, Mark S. Kaiser, Jon D. Larson, Aidas Nasevicius, Karl J. Clark, Shannon A. Wadman, Sharon E. Roberg-Perez, Stephen C. Ekker, Perry B. Hackett, Maura Mcgrail, Jeffrey J. Essner Jan 2010

Moesin1 And Ve-Cadherin Are Required In Endothelial Cells During In Vivo Tubulogenesis, Ying Wang, Mark S. Kaiser, Jon D. Larson, Aidas Nasevicius, Karl J. Clark, Shannon A. Wadman, Sharon E. Roberg-Perez, Stephen C. Ekker, Perry B. Hackett, Maura Mcgrail, Jeffrey J. Essner

Genetics, Development and Cell Biology Publications

Endothelial tubulogenesis is a crucial step in the formation of functional blood vessels during angiogenesis and vasculogenesis. Here, we use in vivo imaging of living zebrafish embryos expressing fluorescent fusion proteins of β-Actin, α-Catenin, and the ERM family member Moesin1 (Moesin a), to define a novel cord hollowing process that occurs during the initial stages of tubulogenesis in intersegmental vessels (ISVs) in the embryo. We show that the primary lumen elongates along cell junctions between at least two endothelial cells during embryonic angiogenesis. Moesin1-EGFP is enriched around structures that resemble intracellular vacuoles, which fuse with the luminal membrane during expansion ...


Skeletal Muscle Stem Cells From Animals I. Basic Cell Biology, Michael V. Dodson, Gary J. Hausman, Leluo Guan, Min Du, Theodore P. Rasmussen, Sylvia P. Poulos, Priya Mir, Werner G. Bergen, Melinda E. Fernyhough, Douglas C. Mcfarland, Robert P. Rhoads, Beatrice Soret, James M. Reecy, Sandra G. Velleman, Zhihua Jiang Jan 2010

Skeletal Muscle Stem Cells From Animals I. Basic Cell Biology, Michael V. Dodson, Gary J. Hausman, Leluo Guan, Min Du, Theodore P. Rasmussen, Sylvia P. Poulos, Priya Mir, Werner G. Bergen, Melinda E. Fernyhough, Douglas C. Mcfarland, Robert P. Rhoads, Beatrice Soret, James M. Reecy, Sandra G. Velleman, Zhihua Jiang

Animal Science Publications

Skeletal muscle stem cells from food-producing animals are of interest to agricultural life scientists seeking to develop a better understanding of the molecular regulation of lean tissue (skeletal muscle protein hypertrophy) and intramuscular fat (marbling) development. Enhanced understanding of muscle stem cell biology and function is essential for developing technologies and strategies to augment the metabolic efficiency and muscle hypertrophy of growing animals potentially leading to greater efficiency and reduced environmental impacts of animal production, while concomitantly improving product uniformity and consumer acceptance and enjoyment of muscle foods.