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Full-Text Articles in Cell Biology

Splenic Endothelial Cell Lines Support Development Of Dendritic Cells From Bone Marrow, Geneviève Despars, Helen O'Neill May 2006

Splenic Endothelial Cell Lines Support Development Of Dendritic Cells From Bone Marrow, Geneviève Despars, Helen O'Neill

Helen O'Neill

Although growth factors are commonly used to generate dendritic cells (DCs) in vitro, the role of the microenvironment necessary for DC development is still poorly understood. The mixed splenic stromal cell population STX3 defines an in vitro microenvironment supportive of DC development. Dissection of cellular components of the STX3 stroma should provide information about a niche for DC development. STX3 was therefore cloned by single-cell sorting, and a panel of 102 splenic stromal cell lines was established. Four representative splenic stromal cell lines that support hematopoiesis from bone marrow are described here in terms of stromal cell type and DC ...


Cell Surface Expression Of A Peptide Encoded By The Unrearranged Tcr-Vβ8.2 Gene, Janice Abbey, Mark Hulett, Helen O'Neill Dec 2005

Cell Surface Expression Of A Peptide Encoded By The Unrearranged Tcr-Vβ8.2 Gene, Janice Abbey, Mark Hulett, Helen O'Neill

Helen O'Neill

Germline transcription of T-cell receptor (TCR) genes has been described in early lymphoid cells. The most common explanation for this phenomenon is that transcription of unrearranged Vβ genes directs gene usage during the rearrangement event. Germline transcription of the TCR-Vβ8.2 gene has been detected in a precursor T-cell line, C1-V13D, which shows no rearrangement at any of the TCR gene loci. This cell line also shows weak binding of specific anti-V 8.2 antibody to the cell surface, consistent with expression of a truncated TCR chain. RT-PCR has been used to confirm expression of spliced germline transcripts of TCR-Vβ8 ...