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Full-Text Articles in Cell Biology

Exogenous Expression Of Human Na, K-Atpase Isoforms In Insect Cells, Christal Renee' Carpenter Apr 2006

Exogenous Expression Of Human Na, K-Atpase Isoforms In Insect Cells, Christal Renee' Carpenter

McCabe Thesis Collection

The Na-K-ATPase or Na pump is a membrane bound enzyme that maintains a high concentration of K+ and low concentration of Na+ in most animal cells. Three subunits constitute the Na pump, named alpha (ex), beta (~). and gamma (y). The ex subunit is responsible for the catalytic and transport of the enzyme, the ~ subunit is important for bringing the ex to the plasma membrane of the cell, and y is a regulatory subunit of the Na pump. In addition, there are several molecular variants or isoforms of the ex and ~ subunits. At present four different ex (ex 1, ex2, ex3 ...


Methods For The Study Of Signaling Molecules In Membrane Lipid Rafts And Caveolae, Rennolds S. Ostrom, Paul A. Insel Jan 2006

Methods For The Study Of Signaling Molecules In Membrane Lipid Rafts And Caveolae, Rennolds S. Ostrom, Paul A. Insel

Pharmacy Faculty Articles and Research

Lipid rafts and caveolae are cholesterol- and sphingolipid-rich microdomains of the plasma membrane that concentrate components of certain signal transduction pathways. Interest in and exploration of these microdomains has grown in recent years, especially after the discovery of the biochemical marker of caveolae, caveolin, and the recognition that caveolin interacts with many different signaling molecules via its scaffolding domain. There are three major types of caveolins (1, 2, and 3), with some selectivity in their expression in different tissues. Results assessing lipid raft/caveolae co-localization of molecules in signal transduction pathways have provided support for the idea that signaling components ...