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Articles 1 - 5 of 5

Full-Text Articles in Cell Biology

Functional Interaction Of The Retinoblastoma And Ini1/Snf5 Tumor Suppressors In Cell Growth And Pituitary Tumorigenesis, Cynthia J. Guidi, Rajini R. Mudhasani, Kathleen Hoover, Andrew Koff, Irwin Leav, Anthony N. Imbalzano, Stephen N. Jones Aug 2006

Functional Interaction Of The Retinoblastoma And Ini1/Snf5 Tumor Suppressors In Cell Growth And Pituitary Tumorigenesis, Cynthia J. Guidi, Rajini R. Mudhasani, Kathleen Hoover, Andrew Koff, Irwin Leav, Anthony N. Imbalzano, Stephen N. Jones

Open Access Articles

The Ini1 subunit of the SWI/SNF chromatin remodeling complex suppresses formation of malignant rhabdoid tumors in humans and mice. Transduction of Ini1 into Ini1-deficient tumor-derived cell lines has indicated that Ini1 arrests cell growth, controls chromosomal ploidy, and suppresses tumorigenesis by regulating components of the retinoblastoma (Rb) signaling pathway. Furthermore, conditional inactivation of Ini1 in mouse fibroblasts alters the expression of various Rb-E2F-regulated genes, indicating that endogenous Ini1 levels may control Rb signaling in cells. We have reported previously that loss of one allele of Ini1 in mouse fibroblasts results only in a 15% to 20% reduction in total ...


Linking Ligand-Induced Alterations In Androgen Receptor Structure To Differential Gene Expression: A First Step In The Rational Design Of Selective Androgen Receptor Modulators, Dmitri Kazmin, Tatiana Prytkova, C. Edgar Cook, Russell Wolfinger, Tzu-Ming Chu, David Beratan, J. D. Norris, Ching-Yi Chang, Donald P. Mcdonnell Jan 2006

Linking Ligand-Induced Alterations In Androgen Receptor Structure To Differential Gene Expression: A First Step In The Rational Design Of Selective Androgen Receptor Modulators, Dmitri Kazmin, Tatiana Prytkova, C. Edgar Cook, Russell Wolfinger, Tzu-Ming Chu, David Beratan, J. D. Norris, Ching-Yi Chang, Donald P. Mcdonnell

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

We have previously identified a family of novel androgen receptor (AR) ligands that, upon binding, enable AR to adopt structures distinct from that observed in the presence of canonical agonists. In this report, we describe the use of these compounds to establish a relationship between AR structure and biological activity with a view to defining a rational approach with which to identify useful selective AR modulators. To this end, we used combinatorial peptide phage display coupled with molecular dynamic structure analysis to identify the surfaces on AR that are exposed specifically in the presence of selected AR ligands. Subsequently, we ...


Comparative Study Of Immunocytochemical Patterns Of Somatotrophs, Mammotrophs, And Mammosomatotrophs In The Porcine Anterior Pituitary , Jin-Sook Lee Jan 2006

Comparative Study Of Immunocytochemical Patterns Of Somatotrophs, Mammotrophs, And Mammosomatotrophs In The Porcine Anterior Pituitary , Jin-Sook Lee

Retrospective Theses and Dissertations

The anterior pituitary gland is a dynamic and complex endocrine gland that synthesizes and secretes trophic hormones from distinct endocrine cells including mammotrophs (prolactin cells or lactotrophs) and somatotrophs (growth hormone cells) to play essential roles in the maintenance of homeostasis, metabolism, reproduction, growth, and lactation. Recently, the existence of a novel anterior pituitary cell type, the mammosomatotroph which contained both growth hormone (GH) and prolactin (PRL) was proposed to function as a transitional cell or progenitor cell between somatotrophs and mammotrophs under various physiological conditions. The present study investigated the spatial distribution patterns of somatotrophs, mammotrophs, and mammosomatotrophs by ...


Understanding Il-12 Responsiveness During Leishmania Amazonensis Infection , Amanda Ellen Ramer Jan 2006

Understanding Il-12 Responsiveness During Leishmania Amazonensis Infection , Amanda Ellen Ramer

Retrospective Theses and Dissertations

Cutaneous leishmaniasis is a vector-borne disease caused by intracellular protozoan parasites of the genus Leishmania. C3H mice challenged with L. major develop a polarized Th1 response and subsequently heal, whereas L. amazonensis challenge leads to chronic lesions with high parasite loads. This work demonstrates that infection with L. amazonensis creates a population of antigen-responsive, CD44hi CD4+ T cells that proliferate and produce IL-2 but do not polarize to an effector phenotype and exhibit limited IFN-gamma production in response to IL-12. CD44 hi CD4+ T cells from L. amazonensis-infected mice fail to accumulate in culture as compared to cells from L ...


Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor Jan 2006

Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor

Retrospective Theses and Dissertations

The purpose of this study is to develop strategies to facilitate nerve regeneration using a synergistic combination of guidance cues. We are investigating the cellular mechanisms of development using adult rat hippocampal progenitor cells (AHPCs) and have demonstrated that manipulating a combination of physical, chemical and biological cues can lead to oriented astrocyte and neural progenitor cell outgrowth and can influence progenitor cell differentiation. To provide physical guidance, micropatterned polystyrene (PS) substrates were fabricated and chemically modified with laminin. Astrocytes or AHPCs cultured on these substrates align along the grooves of the patterned surface extending highly elongated processes. To further ...