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Full-Text Articles in Cell Biology

Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller Aug 2011

Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller

Howard K. Plummer III

Human lung cancer cell lines are widely used to test anticancer drugs. These in-vitro tests, however, preclude the detection of responses to paracrine factors from surrounding stroma. We have cocultured pulmonary fibroblasts CCD-19Lu, from a healthy donor, or HLF-A, from a patient with epidermoid carcinoma of the lung, with two human pulmonary adenocarcinoma cell lines to test the hypothesis that the fibroblasts stimulate the growth of the tumor cells. Both fibroblast cell lines significantly increased the proliferation of the pulmonary adenocarcinoma cell lines in 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide assays, with HLF-A fibroblasts yielding the most pronounced responses ...


Nnk Activates Erk1/2 And Creb/Atf-1 Via Beta-1-Ar And Egfr Signaling In Human Lung Adenocarcinoma And Small Airway Epithelial Cells, E Laag, M Majidi, Maria Cekanova, T Masi, T Takahashi, Hildegard Schuller Sep 2006

Nnk Activates Erk1/2 And Creb/Atf-1 Via Beta-1-Ar And Egfr Signaling In Human Lung Adenocarcinoma And Small Airway Epithelial Cells, E Laag, M Majidi, Maria Cekanova, T Masi, T Takahashi, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

We have shown that the tobacco nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is an agonist for -adrenergic receptors (beta-ARs) and increased DNA synthesis of human lung adenocarcinoma cells with features of bronchiolar Clara cells by binding to these receptors. Using a cell line derived from a human pulmonary adenocarcinoma with Clara cell phenotype (PACC) and immortalized human small airway epithelial cells (HPLD1), the putative cells of origin of this cancer type, our current studies have analyzed signaling initiated by binding of NNK to the beta 1-AR. NNK upregulated ERK1/2 and CREB/ATF-1 phosphorylation in a PKA-dependent manner in ...


Functional Interaction Of The Retinoblastoma And Ini1/Snf5 Tumor Suppressors In Cell Growth And Pituitary Tumorigenesis, Cynthia J. Guidi, Rajini R. Mudhasani, Kathleen Hoover, Andrew Koff, Irwin Leav, Anthony N. Imbalzano, Stephen N. Jones Aug 2006

Functional Interaction Of The Retinoblastoma And Ini1/Snf5 Tumor Suppressors In Cell Growth And Pituitary Tumorigenesis, Cynthia J. Guidi, Rajini R. Mudhasani, Kathleen Hoover, Andrew Koff, Irwin Leav, Anthony N. Imbalzano, Stephen N. Jones

Open Access Articles

The Ini1 subunit of the SWI/SNF chromatin remodeling complex suppresses formation of malignant rhabdoid tumors in humans and mice. Transduction of Ini1 into Ini1-deficient tumor-derived cell lines has indicated that Ini1 arrests cell growth, controls chromosomal ploidy, and suppresses tumorigenesis by regulating components of the retinoblastoma (Rb) signaling pathway. Furthermore, conditional inactivation of Ini1 in mouse fibroblasts alters the expression of various Rb-E2F-regulated genes, indicating that endogenous Ini1 levels may control Rb signaling in cells. We have reported previously that loss of one allele of Ini1 in mouse fibroblasts results only in a 15% to 20% reduction in total ...


Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller Jul 2006

Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

Human lung cancer cell lines are widely used to test anticancer drugs. These in-vitro tests, however, preclude the detection of responses to paracrine factors from surrounding stroma. We have cocultured pulmonary fibroblasts CCD-19Lu, from a healthy donor, or HLF-A, from a patient with epidermoid carcinoma of the lung, with two human pulmonary adenocarcinoma cell lines to test the hypothesis that the fibroblasts stimulate the growth of the tumor cells. Both fibroblast cell lines significantly increased the proliferation of the pulmonary adenocarcinoma cell lines in 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide assays, with HLF-A fibroblasts yielding the most pronounced responses ...


Detection Of Overexpressed Cox-2 In Precancerous Lesions Of Hamster Pancreas And Lungs By Molecular Imaging: Implications For Early Diagnosis And Prevention, Hildegard Schuller, George Kabalka, G Smith, A Mereddy, M Akula, Maria Cekanova May 2006

Detection Of Overexpressed Cox-2 In Precancerous Lesions Of Hamster Pancreas And Lungs By Molecular Imaging: Implications For Early Diagnosis And Prevention, Hildegard Schuller, George Kabalka, G Smith, A Mereddy, M Akula, Maria Cekanova

Maria Cekanova MS, RNDr, PhD

The enzyme cyclooxygenase-2 (COX-2) is overexpressed in many cancers, cardiovascular disease, neurodegenerative disorders, and arthritis. Selective inhibitors of COX-2 have been developed as therapeutics or preventive agents for these diseases. However, recent reports have revealed a significant increase in cardiovascular mortality in long-term users of the COX-2 inhibitors Vioxx and Celebrex, emphasizing the need for noninvasive tests that allow the identification of individuals whose COX-2 levels are overexpressed prior to assignment to treatment with these drugs. In this study, we have prepared a radioiodinated analogue of the selective COX-2 inhibitor celecoxib, and verified its binding to the COX-2 enzyme in ...


Chmp1 Is Implicated In The Development Of Pancreatic Tumor Via The Retinoic Acid Signaling Pathway, Juliana Adedayo Akinsete Jan 2006

Chmp1 Is Implicated In The Development Of Pancreatic Tumor Via The Retinoic Acid Signaling Pathway, Juliana Adedayo Akinsete

Theses, Dissertations and Capstones

In the present study, we investigated the involvement of Chmp1 (Chromatin ModifyingProtein 1/Charged Multivesicular body Protein 1) in the development of mousepancreatic acinar tumor cell line. CRL 2151 cell line was transfected with Chmp1/CS2vector to compare growth, morphology and expression of Chmp1, p53 and pp53 (ser37) with control-transfected cells. CRL 2151 cells were treated with all-trans retinoicacid (ATRA) to compare growth, morphology and expression of Chmp1 and p53 withcontrol-treated cells. Strabismus was used as control. Results showed inhibition ofgrowth but no morphological change in transfected cells. Western blot analysis showedthat Chmp1 transfection upregulated the expression of p53, pp53 ...


Synergistic Mitogenic Signaling Of The Tobacco Specific Carcinogen, 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (Nnk) And Ethanol On Immortalized Human Pancreatic Duct Epithelial Cells, Mdf Askari, Ms Tsao, Maria Cekanova, Hildegard Schuller Dec 2005

Synergistic Mitogenic Signaling Of The Tobacco Specific Carcinogen, 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (Nnk) And Ethanol On Immortalized Human Pancreatic Duct Epithelial Cells, Mdf Askari, Ms Tsao, Maria Cekanova, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

No abstract provided.