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2005

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Articles 1 - 30 of 58

Full-Text Articles in Cell Biology

Maturation Requirements For Dendritic Cells In T Cell Stimulation Leading To Tolerance Versus Immunity, Helen O'Neill, Jonathan Tan Sep 2015

Maturation Requirements For Dendritic Cells In T Cell Stimulation Leading To Tolerance Versus Immunity, Helen O'Neill, Jonathan Tan

Jonathan Tan

The model that dendritic cell (DC) "maturation" describes the change from an immature, antigen-capturing cell to a mature, antigen-presenting cell is well-established. Classification of DCs in terms of function has been problematic previously. It is therefore proposed that mature and not immature DCs are responsible for antigen presentation and stimulation of T cells. Furthermore, DC antigen presentation to T cells can have two outcomes: tolerance or immunity. The particular outcomes appear to be determined by the activation state of the mature DC. DCs can be activated by a range of environmental stimuli or "danger signals". Here, the hypothesis is advanced ...


Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer, Madhu Dhar, Maria Cekanova, Hildegard Schuller Aug 2011

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer, Madhu Dhar, Maria Cekanova, Hildegard Schuller

Howard K. Plummer III

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six ...


Suppression Of Oxidative Metabolism And Mitochondrial Biogenesis By The Transcriptional Corepressor Rip140 In Mouse Adipocytes, Aimee M. Powelka, Asha Seth, Joseph V. Virbasius, Evangelos Kiskinis, Sarah M. Nicoloro, Adilson L. Guilherme, Xiaoqing Tang, Juerg R. Straubhaar, Andrew D. Cherniack, Malcolm G. Parker, Michael P. Czech Dec 2005

Suppression Of Oxidative Metabolism And Mitochondrial Biogenesis By The Transcriptional Corepressor Rip140 In Mouse Adipocytes, Aimee M. Powelka, Asha Seth, Joseph V. Virbasius, Evangelos Kiskinis, Sarah M. Nicoloro, Adilson L. Guilherme, Xiaoqing Tang, Juerg R. Straubhaar, Andrew D. Cherniack, Malcolm G. Parker, Michael P. Czech

Open Access Articles

Using an siRNA-based screen, we identified the transcriptional corepressor RIP140 as a negative regulator of insulin-responsive hexose uptake and oxidative metabolism in 3T3-L1 adipocytes. Affymetrix GeneChip profiling revealed that RIP140 depletion upregulates the expression of clusters of genes in the pathways of glucose uptake, glycolysis, TCA cycle, fatty acid oxidation, mitochondrial biogenesis, and oxidative phosphorylation in these cells. Conversely, we show that reexpression of RIP140 in mouse embryonic fibroblasts derived from RIP140-null mice downregulates expression of many of these same genes. Consistent with these microarray data, RIP140 gene silencing in cultured adipocytes increased both conversion of [14C]glucose to CO2 ...


Soluble Beta-Amyloid1-40 Induces Nmda-Dependent Degradation Of Postsynaptic Density-95 At Glutamatergic Synapses, F. Roselli, M. Tirard, J. Lu, P. Hutzler, P. Lamberti, P. Livrea, Maria A. Morabito, O.F.X. Almeida Dec 2005

Soluble Beta-Amyloid1-40 Induces Nmda-Dependent Degradation Of Postsynaptic Density-95 At Glutamatergic Synapses, F. Roselli, M. Tirard, J. Lu, P. Hutzler, P. Lamberti, P. Livrea, Maria A. Morabito, O.F.X. Almeida

Morabito Lab Publications

Amyloid-beta (Abeta) has been implicated in memory loss and disruption of synaptic plasticity observed in early-stage Alzheimer's disease. Recently, it has been shown that soluble Abeta oligomers target synapses in cultured rat hippocampal neurons, suggesting a direct role of Abeta in the regulation of synaptic structure and function. Postsynaptic density-95 (PSD-95) is a postsynaptic scaffolding protein that plays a critical role in synaptic plasticity and the stabilization of AMPA (AMPARs) and NMDA (NMDARs) receptors at synapses. Here, we show that exposure of cultured cortical neurons to soluble oligomers of Abeta(1-40) reduces PSD-95 protein levels in a dose- and ...


A Ciliary Sensation: Mapping Components Of The Gtp Signaling Pathway, Heather G. Kuruvilla Dec 2005

A Ciliary Sensation: Mapping Components Of The Gtp Signaling Pathway, Heather G. Kuruvilla

Science and Mathematics Faculty Presentations

GTP is a chemorepellent in Tetrahymena thermophila, causing cells to exhibit avoidance behavior, characterized by ciliary reversal. Recent work in our laboratory has shown that tyrosine kinase activity is required in order for GTP signaling to take place (Bartholomew et al., submitted for publication). Second messengers which we have found to be important for GTP signaling in Tetrahymena include nitric oxide and cGMP. Previous studies by Kim et al., 1999, have shown that a calcium-based depolarization is elicited by the application of extracellular GTP. Currently, our lab is addressing the question of where intracellular calcium is involved in the GTP ...


Genetic And Molecular Analysis Of Phytochromes From The Filamentous Fungus Neurospora Crassa, Allan C. Froehlich, Bosl Noh, Richard D. Vierstra, Jennifer Loros, Jay C. Dunlap Dec 2005

Genetic And Molecular Analysis Of Phytochromes From The Filamentous Fungus Neurospora Crassa, Allan C. Froehlich, Bosl Noh, Richard D. Vierstra, Jennifer Loros, Jay C. Dunlap

Open Dartmouth: Faculty Open Access Scholarship

Phytochromes (Phys) comprise a superfamily of red-/far-red-light-sensing proteins. Whereas higher-plant Phys that control numerous growth and developmental processes have been well described, the biochemical characteristics and functions of the microbial forms are largely unknown. Here, we describe analyses of the expression, regulation, and activities of two Phys in the filamentous fungus Neurospora crassa. In addition to containing the signature N-terminal domain predicted to covalently associate with a bilin chromophore, PHY-1 and PHY-2 contain C-terminal histidine kinase and response regulator motifs, implying that they function as hybrid two-component sensor kinases activated by light. A bacterially expressed N-terminal fragment of PHY-2 ...


Chemokine And Chemokine Receptor Expression During Colony Stimulating Factor-1-Induced Osteoclast Differentiation In The Toothless Osteopetrotic Rat: A Key Role For Ccl9 (Mip-1gamma) In Osteoclastogenesis In Vivo And In Vitro, Meilheng Yang, Genevieve Mailhot, Carole A. Mackay, April Mason-Savas, Justin Aubin, Paul R. Odgren Nov 2005

Chemokine And Chemokine Receptor Expression During Colony Stimulating Factor-1-Induced Osteoclast Differentiation In The Toothless Osteopetrotic Rat: A Key Role For Ccl9 (Mip-1gamma) In Osteoclastogenesis In Vivo And In Vitro, Meilheng Yang, Genevieve Mailhot, Carole A. Mackay, April Mason-Savas, Justin Aubin, Paul R. Odgren

Open Access Articles

Osteoclasts differentiate from hematopoietic precursors under systemic and local controls. Chemokines and receptors direct leukocyte traffic throughout the body and may help regulate site-specific bone resorption. We investigated bone gene expression in vivo during rapid osteoclast differentiation induced by colony-stimulating factor 1 (CSF-1) in Csf1-null toothless (tl/tl) rats. Long-bone RNA from CSF-1-treated tl/tl rats was analyzed by high-density microarray over a time course. TRAP (tartrate-resistant acid phosphatase)-positive osteoclasts appeared on day 2, peaked on day 4, and decreased slightly on day 6, as marrow space was expanding. TRAP and cathepsin K mRNA paralleled the cell counts. We ...


The Nit1 Promoter Allows Inducible And Reversible Silencing Of Centrin In Chlamydomonas Reinhardtii, Bettina Koblenz, Karl-Ferdinand Lechtreck Nov 2005

The Nit1 Promoter Allows Inducible And Reversible Silencing Of Centrin In Chlamydomonas Reinhardtii, Bettina Koblenz, Karl-Ferdinand Lechtreck

Open Access Articles

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.


Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, David R. Paoletti, Travis E. Doom, Carissa M. Krane, Michael L. Raymer, Dan E. Krane Nov 2005

Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, David R. Paoletti, Travis E. Doom, Carissa M. Krane, Michael L. Raymer, Dan E. Krane

Biology Faculty Publications

Samples containing DNA from two or more individuals can be difficult to interpret. Even ascertaining the number of contributors can be challenging and associated uncertainties can have dramatic effects on the interpretation of testing results. Using an FBI genotypes dataset, containing complete genotype information from the 13 Combined DNA Index System (CODIS) loci for 959 individuals, all possible mixtures of three individuals were exhaustively and empirically computed. Allele sharing between pairs of individuals in the original dataset, a randomized dataset and datasets of generated cousins and siblings was evaluated as were the number of loci that were necessary to reliably ...


Ablation Of Mekk4 Kinase Activity Causes Neurulation And Skeletal Patterning Defects In The Mouse Embryo, Amy N. Abell, Jaime A. Rivera-Pérez, Bruce D. Cuevas, Mark T. Uhlik, Susan Sather, Nancy L. Johnson, Suzanne K. Minton, Jean M. Lauder, Ann M. Winter-Vann, Kazuhiro Nakamura, Terry Magnuson, Richard R. Vaillancourt, Lynn E. Heasley, Gary L. Johnson Oct 2005

Ablation Of Mekk4 Kinase Activity Causes Neurulation And Skeletal Patterning Defects In The Mouse Embryo, Amy N. Abell, Jaime A. Rivera-Pérez, Bruce D. Cuevas, Mark T. Uhlik, Susan Sather, Nancy L. Johnson, Suzanne K. Minton, Jean M. Lauder, Ann M. Winter-Vann, Kazuhiro Nakamura, Terry Magnuson, Richard R. Vaillancourt, Lynn E. Heasley, Gary L. Johnson

Rivera Lab Publications

Skeletal disorders and neural tube closure defects represent clinically significant human malformations. The signaling networks regulating normal skeletal patterning and neurulation are largely unknown. Targeted mutation of the active site lysine of MEK kinase 4 (MEKK4) produces a kinase-inactive MEKK4 protein (MEKK4(K1361R)). Embryos homozygous for this mutation die at birth as a result of skeletal malformations and neural tube defects. Hindbrains of exencephalic MEKK4(K1361R) embryos show a striking increase in neuroepithelial cell apoptosis and a dramatic loss of phosphorylation of MKK3 and -6, mitogen-activated protein kinase kinases (MKKs) regulated by MEKK4 in the p38 pathway. Phosphorylation of MAPK-activated ...


A Qtl Resource And Comparison Tool For Pigs: Pigqtldb, Zhi-Liang Hu, Svetlana Dracheva, Wonhee Jang, Donna Maglott, John Bastiaansen, Max F. Rothschild, James M. Reecy Oct 2005

A Qtl Resource And Comparison Tool For Pigs: Pigqtldb, Zhi-Liang Hu, Svetlana Dracheva, Wonhee Jang, Donna Maglott, John Bastiaansen, Max F. Rothschild, James M. Reecy

Animal Science Publications

During the past decade, efforts to map quantitative trait loci (QTL) in pigs have resulted in hundreds of QTL being reported for growth, meat quality, reproduction, disease resistance, and other traits. It is a challenge to locate, interpret, and compare QTL results from different studies. We have developed a pig QTL database (PigQTLdb) that integrates available pig QTL data in the public domain, thus, facilitating the use of this QTL data in future studies. We also developed a pig trait classification system to standardize names of traits and to simplify organization and searching of the trait data. These steps made ...


Nitrosamine 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone-Induced Pulmonary Adenocarcinomas In Syrian Golden Hamsters Contain Beta 2-Adrenergic Receptor Single-Nucleotide Polymorphisms, T Masi, Maria Cekanova, K Walker, H Bernart, M Majidi, Jm Becker, Hildegard Schuller Sep 2005

Nitrosamine 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone-Induced Pulmonary Adenocarcinomas In Syrian Golden Hamsters Contain Beta 2-Adrenergic Receptor Single-Nucleotide Polymorphisms, T Masi, Maria Cekanova, K Walker, H Bernart, M Majidi, Jm Becker, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

Cigarette smoking contributes to the development of lung cancer throughout the world, with cases of pulmonary adenocarcinoma (PAC) the most numerous. Nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), which is formed from nicotine, has been demonstrated to cause mutations in genes that affect cell regulation and proliferation. Moreover, NNK has been shown to interact directly with and stimulate beta adrenergic receptor (ADRB) signal transduction pathways. Our goal was to determine whether single-nucleotide polymorphisms (SNPs) in the Adrb2 from PAC tumors were induced in golden hamsters by the injection of NNK. Here we report the cloning and sequencing of Adrb2 clones ...


Osteoclast Differentiation Independent Of The Trance-Rank-Traf6 Axis, Nacksung Kim, Yuho Kadono, Masamichi Takami, Junwon Lee, Seoung-Hoon Lee, Fumihiko Okada, Jung Ha Kim, Takashi Kobayashi, Paul R. Odgren, Hiroyasu Nakano, Wen-Chen Yeh, Sun-Kyeong Lee, Joseph A. Lorenzo, Yongwon Choi Sep 2005

Osteoclast Differentiation Independent Of The Trance-Rank-Traf6 Axis, Nacksung Kim, Yuho Kadono, Masamichi Takami, Junwon Lee, Seoung-Hoon Lee, Fumihiko Okada, Jung Ha Kim, Takashi Kobayashi, Paul R. Odgren, Hiroyasu Nakano, Wen-Chen Yeh, Sun-Kyeong Lee, Joseph A. Lorenzo, Yongwon Choi

Odgren Lab Publications

Osteoclasts are derived from myeloid lineage cells, and their differentiation is supported by various osteotropic factors, including the tumor necrosis factor (TNF) family member TNF-related activation-induced cytokine (TRANCE). Genetic deletion of TRANCE or its receptor, receptor activator of nuclear factor kappaB (RANK), results in severely osteopetrotic mice with no osteoclasts in their bones. TNF receptor-associated factor (TRAF) 6 is a key signaling adaptor for RANK, and its deficiency leads to similar osteopetrosis. Hence, the current paradigm holds that TRANCE-RANK interaction and subsequent signaling via TRAF6 are essential for the generation of functional osteoclasts. Surprisingly, we show that hematopoietic precursors from ...


The Effect Of Ifn-Gamma And Tnf-Alpha On The Nadph Oxidase System Of Human Colostrum Macrophages, Blood Monocytes, And Thp-1 Cells, Ana Carolina Almeida, Jussara Rehder, Silvana Dalge Severino, Jose Martins-Filho, Peter E. Newburger, Antonio Condino-Neto Sep 2005

The Effect Of Ifn-Gamma And Tnf-Alpha On The Nadph Oxidase System Of Human Colostrum Macrophages, Blood Monocytes, And Thp-1 Cells, Ana Carolina Almeida, Jussara Rehder, Silvana Dalge Severino, Jose Martins-Filho, Peter E. Newburger, Antonio Condino-Neto

Hematology/Oncology

The aim of this work was to analyze the effect of Interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) on NADPH oxidase activity and gp91-phox gene expression in human colostrum macrophages (CM), peripheral blood monocytes (PBM), and myelomonocytic THP-1 cells. We also investigated the effect of IFN-gamma on the release of TNF-alpha by these cells. Our results show that under basal culture conditions, CM release more superoxide than PBM and THP-1 cells (p andlt; 0.05). The addition of IFN-gamma, alone or in combination with TNF-alpha, increased spontaneous superoxide release by PBM and THP-1 cells (p andlt; 0.05) and increased ...


Atypical Pkciota Contributes To Poor Prognosis Through Loss Of Apical-Basal Polarity And Cyclin E Overexpression In Ovarian Cancer, Astrid M. Eder, Xiaomei Sui, Daniel G. Rosen, Laura K. Nolden, Kwai Wa Cheng, John P. Lahad, Madhuri Kango-Singh, Karen H. Lu, Carla L. Warneke, Edward N. Atkinson, Isabelle Bedrosian, Khandan Keyomarsi, Wen-Lin Kuo, Joe W. Gray, Jerry C. P. Yin, Jinsong Liu, Georg Halder, Gordon B. Mills Aug 2005

Atypical Pkciota Contributes To Poor Prognosis Through Loss Of Apical-Basal Polarity And Cyclin E Overexpression In Ovarian Cancer, Astrid M. Eder, Xiaomei Sui, Daniel G. Rosen, Laura K. Nolden, Kwai Wa Cheng, John P. Lahad, Madhuri Kango-Singh, Karen H. Lu, Carla L. Warneke, Edward N. Atkinson, Isabelle Bedrosian, Khandan Keyomarsi, Wen-Lin Kuo, Joe W. Gray, Jerry C. P. Yin, Jinsong Liu, Georg Halder, Gordon B. Mills

Biology Faculty Publications

We show that atypical PKCι, which plays a critical role in the establishment and maintenance of epithelial cell polarity, is genomically amplified and overexpressed in serous epithelial ovarian cancers. Furthermore, PKCι protein is markedly increased or mislocalized in all serous ovarian cancers. An increased PKCι DNA copy number is associated with decreased progression-free survival in serous epithelial ovarian cancers. In a Drosophila in vivo epithelial tissue model, overexpression of persistently active atypical PKC results in defects in apical-basal polarity, increased Cyclin E protein expression, and increased proliferation. Similar to the Drosophila model, increased PKCι proteins levels are associated with ...


Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller Aug 2005

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six ...


Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller Aug 2005

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller

Hildegard M. Schuller

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six ...


Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller Aug 2005

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six ...


Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller Aug 2005

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller

Madhu S Dhar

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six ...


Group 13 Hox Proteins Interact With The Mh2 Domain Of R-Smads And Modulate Smad Transcriptional Activation Functions Independent Of Hox Dna Binding Capability, Thomas M. Williams, Melissa E. Williams, Joanne H. Heaton, Thomas D. Gelehrter, Jeffrey W. Innis Aug 2005

Group 13 Hox Proteins Interact With The Mh2 Domain Of R-Smads And Modulate Smad Transcriptional Activation Functions Independent Of Hox Dna Binding Capability, Thomas M. Williams, Melissa E. Williams, Joanne H. Heaton, Thomas D. Gelehrter, Jeffrey W. Innis

Biology Faculty Publications

Interactions with co-factors provide a means by which HOX proteins exert specificity. To identify candidate protein interactors of HOXA13, we created and screened an E11.5–E12.5, distal limb bud yeast two-hybrid prey library. Among the interactors, we isolated the BMP-signaling effector Smad5, which interacted with the paralogous HOXD13 but not with HOXA11 or HOXA9, revealing unique interaction capabilities of the AbdB-like HOX proteins. Using deletion mutants, we determined that the MH2 domain of Smad5 is necessary for HOXA13 interaction.

This is the first report demonstrating an interaction between HOX proteins and the MH2 domain of Smad proteins ...


Pulse Energy Dependence Of Subcellular Dissection By Femtosecond Laser Pulses, A. Heisterkamp, I. Z. Maxwell, E. Mazur, Jean M. Underwood, Jeffrey A. Nickerson, S. Kumar, Donald E. Ingber Jul 2005

Pulse Energy Dependence Of Subcellular Dissection By Femtosecond Laser Pulses, A. Heisterkamp, I. Z. Maxwell, E. Mazur, Jean M. Underwood, Jeffrey A. Nickerson, S. Kumar, Donald E. Ingber

Nickerson Lab Publications

Precise dissection of cells with ultrashort laser pulses requires a clear understanding of how the onset and extent of ablation (i.e., the removal of material) depends on pulse energy. We carried out a systematic study of the energy dependence of the plasma-mediated ablation of fluorescently-labeled subcellular structures in the cytoskeleton and nuclei of fixed endothelial cells using femtosecond, near-infrared laser pulses focused through a high-numerical aperture objective lens (1.4 NA). We find that the energy threshold for photobleaching lies between 0.9 and 1.7 nJ. By comparing the changes in fluorescence with the actual material loss determined ...


Calmodulin And Pf6 Are Components Of A Complex That Localizes To The C1 Microtubule Of The Flagellar Central Apparatus, Matthew J. Wargo, Erin E. Dymek, Elizabeth F. Smith Jul 2005

Calmodulin And Pf6 Are Components Of A Complex That Localizes To The C1 Microtubule Of The Flagellar Central Apparatus, Matthew J. Wargo, Erin E. Dymek, Elizabeth F. Smith

Open Dartmouth: Faculty Open Access Scholarship

Studies of flagellar motility in Chlamydomonas mutants lacking specific central apparatus components have supported the hypothesis that the inherent asymmetry of this structure provides important spatial cues for asymmetric regulation of dynein activity. These studies have also suggested that specific projections associated with the C1 and C2 central tubules make unique contributions to modulating motility; yet, we still do not know the identities of most polypeptides associated with the central tubules. To identify components of the C1a projection, we took an immunoprecipitation approach using antibodies generated against PF6. The pf6 mutant lacks the C1a projection and possesses flagella that only ...


Proteomic Analysis Of A Eukaryotic Cilium, Gregory J. Pazour, Nathan S. Agrin, John D. Leszyk, George B. Witman Jul 2005

Proteomic Analysis Of A Eukaryotic Cilium, Gregory J. Pazour, Nathan S. Agrin, John D. Leszyk, George B. Witman

Open Access Articles

Cilia and flagella are widespread cell organelles that have been highly conserved throughout evolution and play important roles in motility, sensory perception, and the life cycles of eukaryotes ranging from protists to humans. Despite the ubiquity and importance of these organelles, their composition is not well known. Here we use mass spectrometry to identify proteins in purified flagella from the green alga Chlamydomonas reinhardtii. 360 proteins were identified with high confidence, and 292 more with moderate confidence. 97 out of 101 previously known flagellar proteins were found, indicating that this is a very complete dataset. The flagellar proteome is rich ...


Nnk-Induced Hamster Lung Adenocarcinomas Over-Express Beta2-Adrenergic And Egfr Signaling Pathways, Hildegard Schuller, Maria Cekanova Jun 2005

Nnk-Induced Hamster Lung Adenocarcinomas Over-Express Beta2-Adrenergic And Egfr Signaling Pathways, Hildegard Schuller, Maria Cekanova

Maria Cekanova MS, RNDr, PhD

Pulmonary adenocarcinoma (PAC) is the most common type of human lung cancer. A diagnosis of PAC, history of non-smoking and presence of mutations in the EGFR are predictive factors for responsiveness of lung cancer to EGFR-specific tyrosine kinase inhibitors. Unfortunately, less than 50% of PAC cases demonstrate this mutation-based responsiveness. Our immunohistochemical analysis of NNK-induced PAC in hamsters demonstrates the simultaneous over-expression of a beta2-adrenergic receptor pathway, including PKA, cAMP, CREB and phosphorylated CREB and of an EGFR pathway, including over-expression of EGFR-specific phosphorylated tyrosine kinase, Raf-1 and ERK1/2 and their phosphorylated forms. These findings implicate, for the first ...


Characterization Of A Novel Receptor Cs1 In Human Lymphocytes; Studies In Natural Killer Cells And B-Lymphocytes, Jae Kyung Lee M.S. Jun 2005

Characterization Of A Novel Receptor Cs1 In Human Lymphocytes; Studies In Natural Killer Cells And B-Lymphocytes, Jae Kyung Lee M.S.

Theses and Dissertations

The purpose of this study was to investigate the roles of CS1 on human lymphocytes. The molecular and functional characterization of CS1 receptor in the natural killer (NK) cells and B lymphocytes was investigated. CS1 (CRACC, novel Ly9) is a novel member of the CD2 family receptor expressed on natural killer (NK), T cells, activated Bcells and dendritic cells. To examine the existence of isoform of CS1, library from NK cells was screened based on wild type of CS1 (CS1-L). A splice variant form of CS1 (CS1-S), which lacks immunoreceptor tyrosine-based switch motifs (ITSMs) in cytoplasmic domain, was identified. To ...


Predictors Of Serum Dioxin Levels Among Adolescent Boys In Chapaevsk, Russia: A Cross-Sectional Pilot Study, Russ Hauser, Paige L. Williams, Larisa Altshul, Susan Korrick, Lynne Peeples, Donald G. Patterson Jr., Wayman E. Turner, Mary M. Lee, Boris Revich, Oleg Sergeyev May 2005

Predictors Of Serum Dioxin Levels Among Adolescent Boys In Chapaevsk, Russia: A Cross-Sectional Pilot Study, Russ Hauser, Paige L. Williams, Larisa Altshul, Susan Korrick, Lynne Peeples, Donald G. Patterson Jr., Wayman E. Turner, Mary M. Lee, Boris Revich, Oleg Sergeyev

Lee Lab Publications

BACKGROUND: Toxicological studies and limited human studies have demonstrated associations between exposure to polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) and adverse developmental and reproductive health effects. Given that children may be particularly susceptible to reproductive and developmental effects of organochlorines, and the paucity of information available regarding childhood exposures to dioxins in particular, we undertook a pilot study to describe the distribution of, and identify potential predictors of exposure to, dioxin-like compounds and dioxins among adolescent boys in Chapaevsk, Russia. The pilot study was also designed to guide the development of a large prospective cohort study ...


Predictors Of Serum Dioxin Levels Among Adolescent Boys In Chapaevsk, Russia: A Cross-Sectional Pilot Study, Russ Hauser, Paige Williams, Larisa Altshul, Susan Korrick, Lynne Peeples, Donald G. Patterson Jr., Wayman E. Turner, Mary M. Lee, Boris Revich, Oleg Sergeyev May 2005

Predictors Of Serum Dioxin Levels Among Adolescent Boys In Chapaevsk, Russia: A Cross-Sectional Pilot Study, Russ Hauser, Paige Williams, Larisa Altshul, Susan Korrick, Lynne Peeples, Donald G. Patterson Jr., Wayman E. Turner, Mary M. Lee, Boris Revich, Oleg Sergeyev

Lee Lab Publications

BACKGROUND: Toxicological studies and limited human studies have demonstrated associations between exposure to polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) and adverse developmental and reproductive health effects. Given that children may be particularly susceptible to reproductive and developmental effects of organochlorines, and the paucity of information available regarding childhood exposures to dioxins in particular, we undertook a pilot study to describe the distribution of, and identify potential predictors of exposure to, dioxin-like compounds and dioxins among adolescent boys in Chapaevsk, Russia. The pilot study was also designed to guide the development of a large prospective cohort study ...


Device And Method Of Use For Detection And Characterization Of Pathogens And Biological Materials, Eric R. Henderson, Saju R. Nettikadan, Curtis L. Mosher May 2005

Device And Method Of Use For Detection And Characterization Of Pathogens And Biological Materials, Eric R. Henderson, Saju R. Nettikadan, Curtis L. Mosher

Genetics, Development and Cell Biology Patents

The present invention includes a method and apparatus for the detection of a target material. The method and apparatus includes providing a substrate with a surface and forming a domains of deposited materials thereon. The deposited material can be placed on the surface and bound directly and non-specifically to the surface, or it may be specifically or non-specifically bound to the surface. The deposited material has an affinity for a specific target material. The domains thus created are termed affinity domains or deposition domains. Multiple affinity domains of deposited materials can be deposited on a single surface, creating a plurality ...


Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano May 2005

Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano

GSBS Student Publications

The activation of muscle-specific gene expression requires the coordinated action of muscle regulatory proteins and chromatin-remodeling enzymes. Microarray analysis performed in the presence or absence of a dominant-negative BRG1 ATPase demonstrated that approximately one-third of MyoD-induced genes were highly dependent on SWI/SNF enzymes. To understand the mechanism of activation, we performed chromatin immunoprecipitations analyzing the myogenin promoter. We found that H4 hyperacetylation preceded Brg1 binding in a MyoD-dependent manner but that MyoD binding occurred subsequent to H4 modification and Brg1 interaction. In the absence of functional SWI/SNF enzymes, muscle regulatory proteins did not bind to the myogenin promoter ...


Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano May 2005

Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano

Biology Faculty Publications

The activation of muscle-specific gene expression requires the coordinated action of muscle regulatory proteins and chromatin-remodeling enzymes. Microarray analysis performed in the presence or absence of a dominant-negative BRG1 ATPase demonstrated that approximately one-third of MyoD-induced genes were highly dependent on SWI/SNF enzymes. To understand the mechanism of activation, we performed chromatin immunoprecipitations analyzing the myogenin promoter. We found that H4 hyperacetylation preceded Brg1 binding in a MyoD-dependent manner but that MyoD binding occurred subsequent to H4 modification and Brg1 interaction. In the absence of functional SWI/SNF enzymes, muscle regulatory proteins did not bind to the myogenin promoter ...