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2000

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Articles 1 - 30 of 43

Full-Text Articles in Cell Biology

Height Referencing Biochemical Cassette, Marc D. Porter, Vivian W. Jones, Curtis L. Mosher, Eric Henderson Nov 2000

Height Referencing Biochemical Cassette, Marc D. Porter, Vivian W. Jones, Curtis L. Mosher, Eric Henderson

Iowa State University Patents

A height referencing biochemical cassette comprising of a surface suitable to act as a coupling agent, a height referencing indicator, and molecules bonded to the surface. This cassette is a capable of use to test for bonding to these molecules through measuring the height difference between the indicator and the surface. This invention provides an efficient means to quickly and easily test for bonding of molecules to other molecules.


Chlamydomonas Ift88 And Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required For Assembly Of Cilia And Flagella, Gregory J. Pazour, Bethany L. Dickert, Yvonne Vucica, E. Scott Seeley, Joel L. Rosenbaum, George B. Witman, Douglas G. Cole Nov 2000

Chlamydomonas Ift88 And Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required For Assembly Of Cilia And Flagella, Gregory J. Pazour, Bethany L. Dickert, Yvonne Vucica, E. Scott Seeley, Joel L. Rosenbaum, George B. Witman, Douglas G. Cole

Open Access Articles

Intraflagellar transport (IFT) is a rapid movement of multi-subunit protein particles along flagellar microtubules and is required for assembly and maintenance of eukaryotic flagella. We cloned and sequenced a Chlamydomonas cDNA encoding the IFT88 subunit of the IFT particle and identified a Chlamydomonas insertional mutant that is missing this gene. The phenotype of this mutant is normal except for the complete absence of flagella. IFT88 is homologous to mouse and human genes called Tg737. Mice with defects in Tg737 die shortly after birth from polycystic kidney disease. We show that the primary cilia in the kidney of Tg737 mutant mice ...


Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman Oct 2000

Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman

Open Dartmouth: Faculty Open Access Scholarship

Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover ...


Protection Of Telomeres By The Ku Protein In Fission Yeast, Peter Baumann, Thomas R. Cech Oct 2000

Protection Of Telomeres By The Ku Protein In Fission Yeast, Peter Baumann, Thomas R. Cech

Chemistry & Biochemistry Faculty Contributions (1986-2018)

Schizosaccharomyces pombe cells survive loss of telomeres by a unique pathway of chromosome circularization. Factors potentially involved in this survival mechanism include the heterodimeric Ku protein and ligase IV, both of which are involved in the repair of DNA double-strand breaks in mammalian cells. Furthermore, Ku plays a role in telomere maintenance as well as in DNA double-strand break repair in Saccharomyces cerevisiae. We have identified Ku and ligase IV homologues in S. pombe and analyzed their functions during normal growth and in cells undergoing senescence. In the absence of either a Ku subunit (pku70 +) or ligase IV (lig4 +), nonhomologous ...


Interactions Of U2 Gene Loci And Their Nuclear Transcripts With Cajal (Coiled) Bodies: Evidence For Preu2 Within Cajal Bodies, Kelly P. Smith, Jeanne B. Lawrence Sep 2000

Interactions Of U2 Gene Loci And Their Nuclear Transcripts With Cajal (Coiled) Bodies: Evidence For Preu2 Within Cajal Bodies, Kelly P. Smith, Jeanne B. Lawrence

Open Access Articles

The Cajal (coiled) body (CB) is a structure enriched in proteins involved in mRNA, rRNA, and snRNA metabolism. CBs have been shown to interact with specific histone and snRNA gene loci. To examine the potential role of CBs in U2 snRNA metabolism, we used a variety of genomic and oligonucleotide probes to visualize in situ newly synthesized U2 snRNA relative to U2 loci and CBs. Results demonstrate that long spacer sequences between U2 coding repeats are transcribed, supporting other recent evidence that U2 transcription proceeds past the 3' box. The presence of bright foci of this U2 locus RNA differed ...


Tracking Col1a1 Rna In Osteogenesis Imperfecta. Splice-Defective Transcripts Initiate Transport From The Gene But Are Retained Within The Sc35 Domain, Carol V. Johnson, Dragan Primorac, Monique B. Mckinstry, John A. Mcneil, David W. Rowe, Jeanne B. Lawrence Aug 2000

Tracking Col1a1 Rna In Osteogenesis Imperfecta. Splice-Defective Transcripts Initiate Transport From The Gene But Are Retained Within The Sc35 Domain, Carol V. Johnson, Dragan Primorac, Monique B. Mckinstry, John A. Mcneil, David W. Rowe, Jeanne B. Lawrence

Open Access Articles

This study illuminates the intra-nuclear fate of COL1A1 RNA in osteogenesis imperfecta (OI) Type I. Patient fibroblasts were shown to carry a heterozygous defect in splicing of intron 26, blocking mRNA export. Both the normal and mutant allele associated with a nuclear RNA track, a localized accumulation of posttranscriptional RNA emanating to one side of the gene. Both tracks had slightly elongated or globular morphology, but mutant tracks were cytologically distinct in that they lacked the normal polar distribution of intron 26. Normal COL1A1 RNA tracks distribute throughout an SC-35 domain, from the gene at the periphery. Normally, almost all ...


Positive Regulation Of Acetate Metabolism And Motility By The Rna-Binding Protein Csra In Escherichia Coli, Bangdong L. Wei M.B., M.S. Aug 2000

Positive Regulation Of Acetate Metabolism And Motility By The Rna-Binding Protein Csra In Escherichia Coli, Bangdong L. Wei M.B., M.S.

Theses and Dissertations

Wei, Bangdong L., Positive Regulation of Acetate Metabolism and Motility by the RNA-binding Protein CsrA in Escherichia coli. Doctor of Philosophy (Biomedical Sciences), August, 2000, 118 pp., 5 tables, 19 illustrations, bibliography, 175 titles. The carbon storage regulatory (Csr) system consists of a small RNA-binding effector protein, CsrA, and non-coding RNA, CsrB. CsrA acts as a global regulator and modulates specific mRNA stability in Escherichia coli. It regulates central carbon metabolism, physiology, and cell surface properties on a broad scale. In this study, the regulatory roles of csrA in acetate metabolism and motility were examined. The csrA gene was demonstrated ...


Seeking Common Ground In Nuclear Complexity, Lindsay S. Shopland, Jeanne B. Lawrence Jul 2000

Seeking Common Ground In Nuclear Complexity, Lindsay S. Shopland, Jeanne B. Lawrence

Open Access Articles

No abstract provided.


An Ultrastructural Survey Of The Nuchal Glands Of Rhabdophis Tigrinus (Serpentes: Colubridae), Kathleen A. Roberts Jul 2000

An Ultrastructural Survey Of The Nuchal Glands Of Rhabdophis Tigrinus (Serpentes: Colubridae), Kathleen A. Roberts

Biological Sciences Theses & Dissertations

Rhabdophis tigrinus, an Asian natricine snake, possesses a series of integumentary glands located in the nuchal skin. Previous studies indicated that the nuchal glands are of mesodermal origin, contain cardiac steroids analogous to bufotoxins found in the parotoid glands of toads, which are included in the snake's diet. The nuchal glands are defensive in function. Preliminary histological studies revealed differences in quality of the gland tissue compared to previous studies, and difference in fixation techniques were suspected. Seven specimens of R. tigrinus were fixed by perfusion using a conservative fixation process standard for electron microscopy. The nuchal gland series ...


An Outline Of A Theory Of The Constructional Constraints Governing Early Organismic Evolution, Winfried S. Peters, Bernd Herkner Jun 2000

An Outline Of A Theory Of The Constructional Constraints Governing Early Organismic Evolution, Winfried S. Peters, Bernd Herkner

Winfried S. Peters

This paper has no abstract; this is the first paragraph. The origin of life is one of the most fascinating biological enigmas (Dyson 1985, de Duve 1991). A multitude of hypotheses is available, ranging from the classical "primordial broth" (Haldane 1929) to the "genetic takeover" (Cairns-Smith 1982). Most of these models center on biochemical, metabolic, or genetic aspects. To our knowledge a comprehensive investigation into the mechanical constraints governing the early development of organismic constructions is not available to date. Attempting to close this gap, we here present an outline of a theory of the transformation of constructions in early ...


Intracellular Transport, Assembly, And Degradation Of Wild-Type And Disease-Linked Mutant Gap Junction Proteins, Judy K. Vanslyke, Suzanne M. Deschênes, Linda S. Musil Jun 2000

Intracellular Transport, Assembly, And Degradation Of Wild-Type And Disease-Linked Mutant Gap Junction Proteins, Judy K. Vanslyke, Suzanne M. Deschênes, Linda S. Musil

Biology Faculty Publications

More than 130 different mutations in the gap junction integral plasma membrane protein connexin32 (Cx32) have been linked to the human peripheral neuropathy X-linked Charcot–Marie–Tooth disease (CMTX). How these various mutants are processed by the cell and the mechanism(s) by which they cause CMTX are unknown. To address these issues, we have studied the intracellular transport, assembly, and degradation of three CMTX-linked Cx32 mutants stably expressed in PC12 cells. Each mutant had a distinct fate: E208K Cx32 appeared to be retained in the endoplasmic reticulum (ER), whereas both the E186K and R142W mutants were transported to perinuclear ...


The Mechanic State Of “Inner Tissue” In The Growing Zone Of Sunflower Hypocotyls And The Regulation Of Its Growth Rate Following Excision, Winfried Peters, A. Tomos May 2000

The Mechanic State Of “Inner Tissue” In The Growing Zone Of Sunflower Hypocotyls And The Regulation Of Its Growth Rate Following Excision, Winfried Peters, A. Tomos

Winfried S. Peters

Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative growth rates, facilitating comparison to growth in situ. Turgor and turgor-induced strain were determined. Overall longitudinal strain in inner tissues in situ was positive, indicating that compressive forces exerted by peripheral tissues are outweighed by turgor-dependent tensile stress. Inner tissue expansion following isolation depended on water uptake. Extreme plastic extension rates occurred immediately after excision, suggesting ...


Colony-Stimulating Factor-1 (Csf-1) Rescues Osteoblast Attachment, Survival And Sorting Of Beta-Actin Mrna In The Toothless (Tl-Osteopetrotic) Mutation In The Rat, Hiroki Watanabe, Carole A. Mackay, April Mason-Savas, Edward H. Kislauskis, Sandy C. Marks Jr. May 2000

Colony-Stimulating Factor-1 (Csf-1) Rescues Osteoblast Attachment, Survival And Sorting Of Beta-Actin Mrna In The Toothless (Tl-Osteopetrotic) Mutation In The Rat, Hiroki Watanabe, Carole A. Mackay, April Mason-Savas, Edward H. Kislauskis, Sandy C. Marks Jr.

Open Access Articles

We have shown that in the osteopetrotic rat mutation toothless (tl) osteoblasts are absent from older bone surfaces in mutants and that mutant osteoblasts in vivo lack the prominent stress fiber bundles polarized along bone surfaces in osteoblasts from normal littermates. Our recent data demonstrate that in normal osteoblasts in vitro beta- and gamma-actin mRNAs have different, characteristic intracellular distributions and that tl (mutant) osteoblasts fail to differentially sort these mRNAs. Because bone resorption and formation are highly interdependent and injections of CSF-1, a growth factor, increase bone resorption and growth in tl rats, we examined the effects of CSF-1 ...


Ca(2+) Entry Through Store-Operated Channels In Mouse Sperm Is Initiated By Egg Zp3 And Drives The Acrosome Reaction, Christine M. B. O'Toole, Christophe Arnoult, Alberto Darszon, Richard A. Steinhardt, Harvey M. Florman May 2000

Ca(2+) Entry Through Store-Operated Channels In Mouse Sperm Is Initiated By Egg Zp3 And Drives The Acrosome Reaction, Christine M. B. O'Toole, Christophe Arnoult, Alberto Darszon, Richard A. Steinhardt, Harvey M. Florman

Open Access Articles

Fertilization occurs after the completion of the sperm acrosome reaction, a secretory event that is triggered during gamete adhesion. ZP3, an egg zona pellucida glycoprotein, produces a sustained increase of the internal Ca(2+) concentration in mouse sperm, leading to acrosome reactions. Here we show that the sustained Ca(2+) concentration increase is due to the persistent activation of a Ca(2+) influx mechanism during the late stages of ZP3 signal transduction. These cells also possess a Ca(2+) store depletion-activated Ca(2+) entry pathway that is open after treatment with thapsigargin. Thapsigargin and ZP3 activate the same Ca(2 ...


Nuclear Structure-Gene Expression Interrelationships: Implications For Aberrant Gene Expression In Cancer, Gary S. Stein, Martin A. Montecino, Andre J. Van Wijnen, Janet L. Stein, Jane B. Lian Apr 2000

Nuclear Structure-Gene Expression Interrelationships: Implications For Aberrant Gene Expression In Cancer, Gary S. Stein, Martin A. Montecino, Andre J. Van Wijnen, Janet L. Stein, Jane B. Lian

Open Access Articles

There is long-standing recognition that transformed and tumor cells exhibit striking alterations in nuclear morphology as well as in the representation and intranuclear distribution of nucleic acids and regulatory factors. Parameters of nuclear structure support cell growth and phenotypic properties of cells by facilitating the organization of genes, replication and transcription sites, chromatin remodeling complexes, transcripts, and regulatory factors in structurally and functionally definable subnuclear domains within the three-dimensional context of nuclear architecture. The emerging evidence for functional interrelationships of nuclear structure and gene expression is consistent with linkage of tumor-related modifications in nuclear organization to compromised gene regulation during ...


The Morphogenetic Role Of Midline Mesendoderm And Ectoderm In The Development Of The Forebrain And The Midbrain Of The Mouse Embryo, Anne Camus, Bruce P. Davidson, Saraid Billiards, Poh-Lynn Khoo, Jaime A. Rivera-Pérez, Maki Wakamiya, Richard R. Behringer, Patrick P. L. Tam Apr 2000

The Morphogenetic Role Of Midline Mesendoderm And Ectoderm In The Development Of The Forebrain And The Midbrain Of The Mouse Embryo, Anne Camus, Bruce P. Davidson, Saraid Billiards, Poh-Lynn Khoo, Jaime A. Rivera-Pérez, Maki Wakamiya, Richard R. Behringer, Patrick P. L. Tam

Rivera Lab Publications

The anterior midline tissue (AML) of the late gastrula mouse embryo comprises the axial mesendoderm and the ventral neuroectoderm of the prospective forebrain, midbrain and rostral hindbrain. In this study, we have investigated the morphogenetic role of defined segments of the AML by testing their inductive and patterning activity and by assessing the impact of their ablation on the patterning of the neural tube at the early-somite-stage. Both rostral and caudal segments of the AML were found to induce neural gene activity in the host tissue; however, the de novo gene activity did not show any regional characteristic that might ...


Endochondral Bone Formation In Toothless (Osteopetrotic) Rats: Failures Of Chondrocyte Patterning And Type X Collagen Expression, Sandy C. Marks Jr., Carin Lundmark, Cecilia Christersson, Tilmann Wurtz, Paul R. Odgren, Mark F. Seifert, Carole A. Mackay, April Mason-Savas, Steven N. Popoff Apr 2000

Endochondral Bone Formation In Toothless (Osteopetrotic) Rats: Failures Of Chondrocyte Patterning And Type X Collagen Expression, Sandy C. Marks Jr., Carin Lundmark, Cecilia Christersson, Tilmann Wurtz, Paul R. Odgren, Mark F. Seifert, Carole A. Mackay, April Mason-Savas, Steven N. Popoff

Open Access Articles

The pacemaker of endochondral bone growth is cell division and hypertrophy of chondrocytes. The developmental stages of chondrocytes, characterized by the expression of collagen types II and X, are arranged in arrays across the growth zone. Mutations in collagen II and X genes as well as the absence of their gene products lead to different, altered patterns of chondrocyte stages which remain aligned across the growth plate (GP). Here we analyze GP of rats bearing the mutation toothless (tl) which, apart from bone defects, develop a progressive, severe chondrodystrophy during postnatal weeks 3 to 6. Mutant GP exhibited disorganized, non-aligned ...


Mammalian Swi-Snf Complexes Contribute To Activation Of The Hsp70 Gene, Ivana L. De La Serna, Kerri A. Carlson, David A. Hill, Cynthia J. Guidi, Ryan O. Stephenson, Said Sif, Robert E. Kingston, Anthony N. Imbalzano Mar 2000

Mammalian Swi-Snf Complexes Contribute To Activation Of The Hsp70 Gene, Ivana L. De La Serna, Kerri A. Carlson, David A. Hill, Cynthia J. Guidi, Ryan O. Stephenson, Said Sif, Robert E. Kingston, Anthony N. Imbalzano

GSBS Student Publications

ATP-dependent chromatin-remodeling complexes are conserved among all eukaryotes and function by altering nucleosome structure to allow cellular regulatory factors access to the DNA. Mammalian SWI-SNF complexes contain either of two highly conserved ATPase subunits: BRG1 or BRM. To identify cellular genes that require mammalian SWI-SNF complexes for the activation of gene expression, we have generated cell lines that inducibly express mutant forms of the BRG1 or BRM ATPases that are unable to bind and hydrolyze ATP. The mutant subunits physically associate with at least two endogenous members of mammalian SWI-SNF complexes, suggesting that nonfunctional, dominant negative complexes may be formed ...


The Nucleolus And The Four Ribonucleoproteins Of Translation, Thoru Pederson, Joan C. Ritland Politz Mar 2000

The Nucleolus And The Four Ribonucleoproteins Of Translation, Thoru Pederson, Joan C. Ritland Politz

Open Access Articles

No abstract provided.


The Docking Stage Of Yeast Vacuole Fusion Requires The Transfer Of Proteins From A Cis-Snare Complex To A Rab/Ypt Protein, Albert Price, Darren Seals, William Wickner, Christian Ungermann Mar 2000

The Docking Stage Of Yeast Vacuole Fusion Requires The Transfer Of Proteins From A Cis-Snare Complex To A Rab/Ypt Protein, Albert Price, Darren Seals, William Wickner, Christian Ungermann

Open Dartmouth: Faculty Open Access Scholarship

The homotypic fusion of yeast vacuoles requires Sec18p (NSF)-driven priming to allow vacuole docking, but the mechanism that links priming and docking is unknown. We find that a large multisubunit protein called the Vam2/6p complex is bound to cis-paired SNAP receptors (SNAREs) on isolated vacuoles. This association of the Vam2/6p complex with the cis-SNARE complex is disrupted during priming. The Vam2/6p complex then binds to Ypt7p, a guanosine triphosphate binding protein of the Rab family, to initiate productive contact between vacuoles. Thus, cis-SNARE complexes can contain Rab/Ypt effectors, and these effectors can be mobilized by ...


Ets-2 Is A Target For An Akt (Protein Kinase B)/Jun N-Terminal Kinase Signaling Pathway In Macrophages Of Motheaten-Viable Mutant Mice, Alicia E. Schaffner, James L. Smith, Joseph K. Hofmeister, Matthew Hartman, Guo Wei, David Forsthoefel, David A. Hume, Michael C. Ostrowski Mar 2000

Ets-2 Is A Target For An Akt (Protein Kinase B)/Jun N-Terminal Kinase Signaling Pathway In Macrophages Of Motheaten-Viable Mutant Mice, Alicia E. Schaffner, James L. Smith, Joseph K. Hofmeister, Matthew Hartman, Guo Wei, David Forsthoefel, David A. Hume, Michael C. Ostrowski

Science and Mathematics Faculty Publications

The transcription factor ets-2 was phosphorylated at residue threonine 72 in a colony-stimulating factor 1 (CSF-1)- and mitogen-activated protein kinase-independent manner in macrophages isolated from motheaten-viable (me-v) mice. The CSF-1 and ets-2 target genes coding for Bcl-x, urokinase plasminogen activator, and scavenger receptor were also expressed at high levels independent of CSF-1 addition to me-v cells. Akt (protein kinase B) was constitutively active in me-v macrophages, and an Akt immunoprecipitate catalyzed phosphorylation of ets-2 at threonine 72. The p54 isoform of c-jun N-terminal kinase–stress-activated kinase (JNK- SAPK) coimmunoprecipitated with Akt from me-v macrophages, and treatment ofme-v cells with ...


Roles Of Hof1p, Bni1p, Bnr1p, And Myo1p In Cytokinesis In Saccharomyces Cerevisiae, Elizabeth Ann Vallen, J. Caviston, E. Bi Feb 2000

Roles Of Hof1p, Bni1p, Bnr1p, And Myo1p In Cytokinesis In Saccharomyces Cerevisiae, Elizabeth Ann Vallen, J. Caviston, E. Bi

Biology Faculty Works

Cytokinesis in Saccharomyces cerevisiae occurs by the concerted action of the actomyosin system and septum formation. Here we report on the roles of HOF1,BNI1, and BNR1 in cytokinesis, focusing on Hof1p. Deletion of HOF1 causes a temperature-sensitive defect in septum formation. A Hof1p ring forms on the mother side of the bud neck in G2/M, followed by the formation of a daughter-side ring. Around telophase, Hof1p is phosphorylated and the double rings merge into a single ring that contracts slightly and may colocalize with the actomyosin structure. Upon septum formation, Hof1p splits into two rings, disappearing upon cell ...


What Makes Plants Different? Principles Of Extracellular Matrix Function In 'Soft' Plant Tissues [Review Article], Winfried Peters, Wolfgang Hagemann, A. Tomos Jan 2000

What Makes Plants Different? Principles Of Extracellular Matrix Function In 'Soft' Plant Tissues [Review Article], Winfried Peters, Wolfgang Hagemann, A. Tomos

Winfried S. Peters

An overview of the biomechanic and morphogenetic function of the plant extracellular matrix (ECM) in its primary state is given. ECMs can play a pivotal role in cellular osmo- and volume-regulation, if they enclose the cell hermetically and constrain hydrostatic pressure evoked by osmotic gradients between the cell and its environment. From an engineering viewpoint, such cell walls turn cells into hydraulic machines, which establishes a crucial functional differences between cell walls and other cellular surface structures. Examples of such hydraulic machineries are discussed. The function of cell walls in the control of pressure, volume, and shape establishes constructional evolutionary ...


The Nuclear Factor Of Activated T Cells (Nfat) Transcription Factor Nfatp (Nfatc2) Is A Repressor Of Chondrogenesis, Ann M. Ranger, Louis C. Gerstenfeld, Jinxi Wang, Tamiyo Kon, Hyunsu Bae, Ellen M. Gravallese, Melvin J. Glimcher, Laurie H. Glimcher Jan 2000

The Nuclear Factor Of Activated T Cells (Nfat) Transcription Factor Nfatp (Nfatc2) Is A Repressor Of Chondrogenesis, Ann M. Ranger, Louis C. Gerstenfeld, Jinxi Wang, Tamiyo Kon, Hyunsu Bae, Ellen M. Gravallese, Melvin J. Glimcher, Laurie H. Glimcher

Rheumatology Publications and Presentations

Nuclear factor of activated T cells (NFAT) transcription factors regulate gene expression in lymphocytes and control cardiac valve formation. Here, we report that NFATp regulates chondrogenesis in the adult animal. In mice lacking NFATp, resident cells in the extraarticular connective tissues spontaneously differentiate to cartilage. These cartilage cells progressively differentiate and the tissue undergoes endochondral ossification, recapitulating the development of endochondral bone. Proliferation of already existing articular cartilage cells also occurs in some older animals. At both sites, neoplastic changes in the cartilage cells occur. Consistent with these data, NFATp expression is regulated in mesenchymal stem cells induced to differentiate ...


The Skn7 Response Regulator Of Saccharomyces Cerevisiae Interacts With Hsf1 In Vivo And Is Required For The Induction Of Heat Shock Genes By Oxidative Stress, Desmond C. Raitt, Anthony L. Johnson, Alexander M. Erkine, Kozo Makino, Brian Morgan, David S. Gross, Leland H. Johnston Jan 2000

The Skn7 Response Regulator Of Saccharomyces Cerevisiae Interacts With Hsf1 In Vivo And Is Required For The Induction Of Heat Shock Genes By Oxidative Stress, Desmond C. Raitt, Anthony L. Johnson, Alexander M. Erkine, Kozo Makino, Brian Morgan, David S. Gross, Leland H. Johnston

Scholarship and Professional Work – COPHS

The Skn7 response regulator has previously been shown to play a role in the induction of stress-responsive genes in yeast, e.g., in the induction of the thioredoxin gene in response to hydrogen peroxide. The yeast Heat Shock Factor, Hsf1, is central to the induction of another set of stress-inducible genes, namely the heat shock genes. These two regulatory trans-activators, Hsf1 and Skn7, share certain structural homologies, particularly in their DNA-binding domains and the presence of adjacent regions of coiled-coil structure, which are known to mediate protein–protein interactions. Here, we provide evidence that Hsf1 and Skn7 interact in ...


Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross Jan 2000

Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross

Scholarship and Professional Work – COPHS

In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G1-arrested cells ...


Dietary Conjugated Linoleic Acid Expands Cd8+ Lymphocyte Subsets In Vivo And Enhances Their Function , Josep Bassaganya-Riera Jan 2000

Dietary Conjugated Linoleic Acid Expands Cd8+ Lymphocyte Subsets In Vivo And Enhances Their Function , Josep Bassaganya-Riera

Retrospective Theses and Dissertations

In the first experiment, 64 pigs were used in a factorial (2 x 4) arrangement within a split-plot design with eight blocks of four littermate pigs as the experimental unit for the environment and pig within litter as the experimental unit for dietary treatment to evaluate the effects of conjugated linoleic acid (CLA) on growth and immune cell phenotype when pigs were located in two distinct health status environments. Diets were formulated to contain CLA at 0, .67, 1.33 or 2% and to exceed NRC (1988) nutrient needs of pigs and were self-fed for 7 wk in three phases ...


Two Tetrahymena G-Dna-Binding Proteins, Tgp1 And Tgp3, Share Novel Motifs And May Play A Role In Micronuclear Division, Quan Lu, Eric Henderson Jan 2000

Two Tetrahymena G-Dna-Binding Proteins, Tgp1 And Tgp3, Share Novel Motifs And May Play A Role In Micronuclear Division, Quan Lu, Eric Henderson

Zoology and Genetics Publications

G-DNA is a four-stranded DNA structure with diverse putative biological roles. We have previously purified and cloned a novel G-DNA-binding protein TGP1 from the ciliate Tetrahymena thermophila. Here we report the molecular cloning of TGP3, an additional G-DNA-binding protein from the same organism. The TGP3 cDNA encodes a 365 amino acid protein that is homologous to TGP1 (34% identity and 44% similarity). The proteins share a sequence pattern that contains two novel repetitive and homologous motifs flanking an extensively hydrophilic and basic region. A nuclear fractionation experiment showed that TGP1 and TGP3 activities are localized predominantly in the nuclear fraction ...


Mechanisms Of Negative Regulation Of Protein Prenylation: Investigation Of Murine Guanylate-Binding Protein 1 , John Thomas Stickney Jan 2000

Mechanisms Of Negative Regulation Of Protein Prenylation: Investigation Of Murine Guanylate-Binding Protein 1 , John Thomas Stickney

Retrospective Theses and Dissertations

Covalent attachment of isoprenoid lipids to proteins is a post-translational modification that occurs on approximately 2% of all cellular proteins. Proteins that contain a CaaX motif as their final four amino acids are presumed to be targets for the addition of C15 or C20 isoprenoids by farnesyl transferase (FTase) or geranylgeranyl transferase I (GGTase I) respectively. This modification is generally believed to occur stoichiometrically, and irreversibly, in vivo. Thus, if prenylation is to be regulated, this control must occur prior to lipid addition. At the moment, there is little evidence that the enzymatic activity of prenyl transferases can be modulated ...


Tissue Specific Expression And Regulation Of The Mitogen Regulated Protein Genes , John Tollen Fassett Jan 2000

Tissue Specific Expression And Regulation Of The Mitogen Regulated Protein Genes , John Tollen Fassett

Retrospective Theses and Dissertations

Mitogen Regulated Protein (MRP; also called Proliferin; or MRP/PLF) is a member of the Prolactin/Growth Hormone gene family. There is evidence that MRP/PLF is a uterine growth factor and an angiogenesis factor. Between four and six highly homologous MRP/PLF genes (mrp/plf ) exist in the mouse, but the significance of multiple genes has not been determined. MRP/Plf is produced at high levels during mid-gestation by the placenta, where expression is restricted to the trophoblastic giant cells. To better understand the regulation of the mrp/ plf genes in vivo, we have developed an assay to analyze ...