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Cell Biology Commons

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1996

Cell biology

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Full-Text Articles in Cell Biology

The Role Of Rat Brain Ptp-1 Surface Basic Residues In Substrate Reactivity , Deborah Sue Baedke Jan 1996

The Role Of Rat Brain Ptp-1 Surface Basic Residues In Substrate Reactivity , Deborah Sue Baedke

Retrospective Theses and Dissertations

Previous work by others has demonstrated that rat brain protein tyrosine phosphatase (rbPTP-1) exhibited increased reactivity toward substrates containing acidic residues N-terminal to phosphotyrosine. We show that substrates containing a single acidic residue C-terminal to the phosphotyrosine also enhanced reactivity with rbPTP-1. A substrate aspartate residue at the P+2 position exhibited the greatest enhancement of reactivity;Surface basic residues of rbPTP-1 were investigated for their role in the increased reactivity due to substrate acidic residues. Mutation of R47 or K120 to alanine decreased the enhancement of reactivity due to acidic residues N-terminal to phosphotyrosine by 78% and 73% respectively ...