Open Access. Powered by Scholars. Published by Universities.®

Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Cell Biology

Mitochondrial Dna Of The Thatching Ant, Formica Obscuripes, Gregory Park Apr 1996

Mitochondrial Dna Of The Thatching Ant, Formica Obscuripes, Gregory Park

Life and Environmental Sciences Undergraduate Theses

In a continuing attempt to purify mitochondrial DNA from the thatching ant, modifications were made to a previously developed extraction procedure. Although mitochondria were highly enriched in a subcellular fraction derived from the heads of the ants, when DNA purified from the fraction by an alkaline lysis procedure was electrophoresed, inconsistent results were obtained. Bands in the gel show a molecule larger than 22 kilobase pairs, which is not expected of mtDNA.


A Comparative Study Of The Binding Of Two Lectins In The Early Development Of The Freshwater Sponge, Ephydatia Mulleri, Jay Taylor Apr 1996

A Comparative Study Of The Binding Of Two Lectins In The Early Development Of The Freshwater Sponge, Ephydatia Mulleri, Jay Taylor

Life and Environmental Sciences Undergraduate Theses

Carbohydrates have been implicated in cell adhesion and in developmental processes. Lectin histochemistry using ConcanavalinA and Lotus lectin was used to localize lectin-accessible mannosyl and fucosyl residues in a maturing sponge developing from histoblasts generated during germination of a gemmule. With the exception of cystencytes found in the sponges stained with Lotus lectin, a similar staining pattern was seen with the two lectins, but there was a rather significant difference in the intensity of staining. This difference in staining intensity was thought to be a result of either more mannosyl residues available to the lectin or that the concentration of ...


Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon Apr 1996

Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon

Life and Environmental Sciences Undergraduate Theses

This project was intended to be a pair of preliminary experiments from which more detailed and specific experiments could be expanded. There were two procedures used in carrying out this study involving the isolation of DNA from a freshwater sponge. First, genomic DNA was isolated and purified. This genomic DNA was digested with the restriction endonuclease EcoR1 and then analyzed using agarose gel electrophoresis. The results showed an accumulation of the sponge DNA in one major band roughly slightly larger than the 21226 bp band generated by EcoR1 digestion of X-phage DNA. The second part of this experiment was an ...