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Full-Text Articles in Cell Biology

Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon Apr 1996

Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon

Life and Environmental Sciences Undergraduate Theses

This project was intended to be a pair of preliminary experiments from which more detailed and specific experiments could be expanded. There were two procedures used in carrying out this study involving the isolation of DNA from a freshwater sponge. First, genomic DNA was isolated and purified. This genomic DNA was digested with the restriction endonuclease EcoR1 and then analyzed using agarose gel electrophoresis. The results showed an accumulation of the sponge DNA in one major band roughly slightly larger than the 21226 bp band generated by EcoR1 digestion of X-phage DNA. The second part of this experiment was an ...


Regulation Of Dna Replication In Xenopus Laevis , Jing Fang Jan 1996

Regulation Of Dna Replication In Xenopus Laevis , Jing Fang

Retrospective Theses and Dissertations

The purpose of my research project is to make an attempt to understand some aspects of eukaryotic DNA replication by studying how this process is regulated positively or negatively in different physiological conditions. Using Xenopus egg extracts as the in vitro replication system, I studied the inhibitors of DNA replication found in nuclei of Xenopus quiescent cells. Replication of nuclei isolated from quiescent Xenopus cells was barely detectable in interphase extracts of Xenopus laevis eggs, although Xenopus sperm chromatin was replicated with about 100% efficiency in the same extracts. The evidence of the inhibitors came further from the fact that ...