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Full-Text Articles in Cell Biology

Ultrastructural Characterization Of Colloidal Metal Films For Bioanalytical Applications By Scanning Force Microscopy, Wolfgang Fritzsche, Konstantin Sokolov, George D. Chumanov, Therese M. Cotton, Eric Henderson Jan 1996

Ultrastructural Characterization Of Colloidal Metal Films For Bioanalytical Applications By Scanning Force Microscopy, Wolfgang Fritzsche, Konstantin Sokolov, George D. Chumanov, Therese M. Cotton, Eric Henderson

Zoology and Genetics Publications

Colloidal metal films (CMFs) are prepared by the attachment of silver, gold, or platinum (or other metal) particles to a glass slide modified by silanization with 3‐mercaptopropyl silane. The covalent attachment of the metal particles occurs through the metal–sulfur bond. In these samples the local electromagnetic field is enhanced near the surface of the CMF due to excitation of plasmon resonances. This phenomenon can be used for a variety of analytical applications. Because the optical properties are strongly dependent on the morphology of the film, its structural characterization becomes of great importance. To further characterize CMFs we have ...


Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard Jan 1996

Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard

Retrospective Theses and Dissertations

Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. 275-nm excitation gave attomole (amol = 10-18 mol) detection limits for the intracellular species of interest. Two projects are described in which hemoglobin (Hb) variants were determined in single red blood cells. In the third experiment, individual mast cells were degranulated on-column, and exocytosis and serotonin release were monitored temporally;First, single red blood cells, in which Hb ...