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1994

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Articles 1 - 27 of 27

Full-Text Articles in Cell Biology

Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao Dec 1994

Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao

Electronic Theses and Dissertations

Using conventional microelectrodes, I studied the electrical basis for determining the resting V$\sb{\rm m}$ in intact EC's from hamsters. The resting V$\sb{\rm m}$ were found to be $-$40 mV for aortic EC's and $-$43 mV for vena caval EC's. The contributions of ions to the resting V$\sb{\rm m}$ of aortic EC's were compared in terms of the transference number (t$\sb{\rm ion}$). To develop a technique for in situ monitoring changes in V$\sb{\rm m}$ of postcapillary venular EC's in the hamster mesentery, a voltage-sensitive fluorescent probe ...


The Effects Of Elevated Glucose Upon Na+/K+-Atpase In Bovine Retinal Pigment Epithelial Cells, Julie Y. Crider Dec 1994

The Effects Of Elevated Glucose Upon Na+/K+-Atpase In Bovine Retinal Pigment Epithelial Cells, Julie Y. Crider

Theses and Dissertations

Crider, Julie Y., The Effects of Elevated Glucose Upon Na+/K+-ATPase in Bovine Retinal Pigment Epithelial Cells. Doctor of Philosophy (Biomedical Sciences, Pharmacology), December, 1994, 154 pp., 14 tables, 31 illustrations, bibliography, 288 titles. Bovine retinal pigment epithelial (RPE) cells were cultured under 1, 4.5 and 10 g/l glucose conditions in order to characterize the effects of hyperglycemia upon Na+/K+-ATPase. Functional activity of Na+/K+-ATPase was measured as ouabain-sensitive Rb+ uptake. 3H ouabain was used to assess binding characteristics of Na+/K+-ATPase. The major contributors to rubidium (mRb+) uptake activity were the ouabain-sensitive ...


Localization Of Individual Calcium Channels At The Release Face Of A Presynaptic Nerve Terminal, Philip G. Haydon, Eric Henderson, Elis F. Stanley Dec 1994

Localization Of Individual Calcium Channels At The Release Face Of A Presynaptic Nerve Terminal, Philip G. Haydon, Eric Henderson, Elis F. Stanley

Zoology and Genetics Publications

Studies using biophysical techniques suggest a highly structured organization of calcium channels at the presynaptic transmitter release face (Llinás et al., 1981; Stanley, 1993), but it has not as yet proved possible to localize identified channels at the required nanometer level of resolution. We have used atomic force microscopy on the calyx-type nerve terminal of the chick ciliary ganglion to localize single calcium channels tagged via biotinylated ω-conotoxin GVIA to avidin-coated 30 nm gold particles. Calcium channels were in low (modal value approximately ⩽ 1 per μm2) and high (modal value55 per μm2) density areas and exhibited a prominent ...


Mediation Of Chemoattractant-Induced Changes In [Ca2+]I And Cell Shape, Polarity, And Locomotion By Insp3, Dag, And Protein Kinase C In Newt Eosinophils, Susan H. Gilbert, Kristine Perry, Fredric S. Fay Oct 1994

Mediation Of Chemoattractant-Induced Changes In [Ca2+]I And Cell Shape, Polarity, And Locomotion By Insp3, Dag, And Protein Kinase C In Newt Eosinophils, Susan H. Gilbert, Kristine Perry, Fredric S. Fay

Open Access Articles

During chemotaxis large eosinophils from newts exhibit a gradient of [Ca2+]i from rear to front. The direction of the gradient changes on relocation of the chemoattractant source, suggesting that the Ca2+ signal may trigger the cytoskeletal reorganization required for cell reorientation during chemotaxis. The initial stimulatory effect of chemoattractant on [Ca2+]i and the opposite orientations of the intracellular Ca2+ gradient and the external stimulus gradient suggest that more than one chemoattractant-sensitive messenger pathway may be responsible for the generation of spatially graded Ca2+ signals. To identify these messengers, Ca2+ changes were measured in single live cells stimulated with ...


Sequences Responsible For Intracellular Localization Of Beta-Actin Messenger Rna Also Affect Cell Phenotype, Edward H. Kislauskis, Xiaochun Zhu, Robert H. Singer Oct 1994

Sequences Responsible For Intracellular Localization Of Beta-Actin Messenger Rna Also Affect Cell Phenotype, Edward H. Kislauskis, Xiaochun Zhu, Robert H. Singer

Open Access Articles

We have characterized the structure and function of RNA sequences that direct beta-cytoplasmic actin mRNA to the cell periphery were mapped to two segments of 3'-untranslated region by expression of LacZ/beta-actin chimeric mRNAs in chicken embryo fibroblasts (CEFs). A 54-nt segment, the "RNA zipcode," and a homologous but less active 43-nt segment each localized beta-galactosidase activity to the leading lamellae. This zipcode contains the full activity, and mutations or deletions within it reduce, but do not eliminate, its activity, indicating that several motifs contribute to the activity. Two of these motifs, when multimerized, can regenerate almost full activity ...


In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski Oct 1994

In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski

Theses and Dissertations in Biomedical Sciences

The 85 kDa cytosolic phospholipase A2 (cPLA2) is an agonist-responsive effector for intracellular signal transduction through the arachidonate cascade. In vitro studies have demonstrated that this enzyme is regulated by sub-micromolar calcium and is specific for arachidonate as the sn-2 fatty acyl group of phospholipid substrates. However, very little data is available regarding in situ mechanisms which govern the activity of cPLA2. The primarily objective of these studies was to develop an in situ system for the study of cPLA2, and investigate mobilization of arachidonate during signal transduction events.

Dimethylsulfoxide differentiation of the human lymphoma cell ...


Ponticulin Is The Major High Affinity Link Between The Plasma Membrane And The Cortical Actin Network In Dictyostelium, Anne L. Hitt, J. H. Hartwig, Elizabeth J. Luna Sep 1994

Ponticulin Is The Major High Affinity Link Between The Plasma Membrane And The Cortical Actin Network In Dictyostelium, Anne L. Hitt, J. H. Hartwig, Elizabeth J. Luna

Women’s Health Research Faculty Publications

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization and stability, the control of cell shape, and various motile processes. To ascertain the function of high affinity actin-membrane associations, we have disrupted by homologous recombination the gene encoding ponticulin, the major high affinity actin-membrane link in Dictyostelium discoideum amoebae. Cells lacking detectable amounts of ponticulin message and protein also are deficient in high affinity actin-membrane binding by several criteria. First, only 10-13% as much endogenous actin cosediments through sucrose and crude plasma membranes from ponticulin-minus cells, as compared with membranes from the parental strain ...


Association Of The Dictyostelium 30 Kda Actin Bundling Protein With Contact Regions, M. Fechheimer, H. M. Ingalls, R. Furukawa, Elizabeth J. Luna Sep 1994

Association Of The Dictyostelium 30 Kda Actin Bundling Protein With Contact Regions, M. Fechheimer, H. M. Ingalls, R. Furukawa, Elizabeth J. Luna

Women’s Health Research Faculty Publications

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium amebae (H.M. Ingalls et al. (1986). Proc. Nat. Acad. Sci. USA 83, 4779). Purified contact regions contain a prominent actin-binding protein with an M(r) of 34,000. Immunoblotting with monoclonal antibodies identifies this polypeptide as a 34,000 M(r) actin-bundling protein (known as 30 kDa protein), previously shown to be enriched in filopodia (M. Fechheimer (1987). J. Cell Biol. 104, 1539). About four times more 30 kDa protein by mass is associated with contact regions than is found in total plasma membranes ...


Ponticulin Is An Atypical Membrane Protein, Anne L. Hitt, Tze Hong Lu, Elizabeth J. Luna Sep 1994

Ponticulin Is An Atypical Membrane Protein, Anne L. Hitt, Tze Hong Lu, Elizabeth J. Luna

Women’s Health Research Faculty Publications

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and nucleates actin assembly. A single copy gene encodes a developmentally regulated message that is high during growth and early development, but drops precipitously during cell streaming at approximately 8 h of development. The deduced amino acid sequence predicts a protein with a cleaved NH2-terminal signal sequence and a COOH-terminal glycosyl anchor. These predictions are supported by amino acid sequencing of mature ponticulin and metabolic labeling with glycosyl anchor components. Although no alpha-helical membrane-spanning domains are apparent, several hydrophobic and/or sided beta-strands, each long ...


A Double Leucine Within The Glut4 Glucose Transporter Cooh-Terminal Domain Functions As An Endocytosis Signal, Silvia Corvera, Anil Chawla, Ranjan Chakrabarti, Marguerite Joly, Joanne M. Buxton, Michael P. Czech Aug 1994

A Double Leucine Within The Glut4 Glucose Transporter Cooh-Terminal Domain Functions As An Endocytosis Signal, Silvia Corvera, Anil Chawla, Ranjan Chakrabarti, Marguerite Joly, Joanne M. Buxton, Michael P. Czech

Open Access Articles

The unique COOH-terminal 30-amino acid region of the adipocyte/skeletal muscle glucose transporter (GLUT4) appears to be a major structural determinant of this protein's perinuclear localization, from where it is redistributed to the cell surface in response to insulin. To test whether an underlying mechanism of this domain's function involves glucose transporter endocytosis rates, transfected cells were generated expressing exofacial hemagglutinin epitope (HA)-tagged erythrocyte/brain glucose transporter (GLUT1) or a chimera containing the COOH-terminal 30 amino acids of GLUT4 substituted onto this GLUT1 construct. Incubation of COS-7 or CHO cells expressing the HA-tagged chimera with anti-HA antibody ...


Dynamic Changes In The Higher-Level Chromatin Organization Of Specific Sequences Revealed By In Situ Hybridization To Nuclear Halos, Michael G. Gerdes, Kenneth C. Carter, Phillip T. Moen, Jeanne B. Lawrence Jul 1994

Dynamic Changes In The Higher-Level Chromatin Organization Of Specific Sequences Revealed By In Situ Hybridization To Nuclear Halos, Michael G. Gerdes, Kenneth C. Carter, Phillip T. Moen, Jeanne B. Lawrence

Open Access Articles

A novel approach to study the higher level packaging of specific DNA sequences has been developed by coupling high-resolution fluorescence hybridization with biochemical fractionation to remove histones and distend DNA loops to form morphologically reproducible nuclear "halos." Results demonstrate consistent differences in the organization of specific sequences, and further suggest a relationship to functional activity. Pulse-incorporated bromodeoxyuridine representing nascent replicating DNA localized with the base of the chromatin loops in discrete clustered patterns characteristic of intact cells, whereas at increasing chase times, the replicated DNA was consistently found further out on the extended region of the halo. Fluorescence hybridization to ...


Mutations In The Non-Helical Linker Segment L1-2 Of Keratin 5 In Patients With Weber-Cockayne Epidermolysis Bullosa Simplex, Yiu-Mo Chan, Qian-Chun Yu, Janine M. Leblanc-Straceski, Angela Christiano, Lena Pulkkinen, Raju S. Kucherlapati, Jouni Uitto, Elaine Fuchs Apr 1994

Mutations In The Non-Helical Linker Segment L1-2 Of Keratin 5 In Patients With Weber-Cockayne Epidermolysis Bullosa Simplex, Yiu-Mo Chan, Qian-Chun Yu, Janine M. Leblanc-Straceski, Angela Christiano, Lena Pulkkinen, Raju S. Kucherlapati, Jouni Uitto, Elaine Fuchs

Biology Faculty Publications

Keratins are the major structural proteins of the epidermis. Analyzing keratin gene sequences, appreciating the switch in keratin gene expression that takes place as epidermal cells commit to terminally differentiate, and elucidating how keratins assemble into 10 nm filaments, have provided the foundation that has led to the discoveries of the genetic bases of two major classes of human skin diseases, epidermolysis bullosa simplex (EBS) and epidermolytic hyperkeratosis (EH). These diseases involve point mutations in either the basal epidermal keratin pair, K5 and K14 (EBS), or the suprabasal pair, K1 and K10 (EH). In severe cases of EBS and EH ...


Analysis Of Human Sperm Chromatin Integrity, Silvina M. Bocca Apr 1994

Analysis Of Human Sperm Chromatin Integrity, Silvina M. Bocca

Theses and Dissertations in Biomedical Sciences

Determining potential maternal or paternal sources of abnormal chromosomal constitution gives opportunity for preconception genetic counseling. The most direct determination is achieved by analyzing the nuclear constitution of the gametes.

The present study evaluated the integrity of human spermatozoal nuclear material in the two condensation stages of chromatin and chromosomes. Original semen samples (ORI) and their swim-up fractions (SW, selected for motility) from men of known (donors) and unknown (patients) fertility were analyzed. The extent of chromatin condensation was assessed by light microscopy and flow cytometry during the time course of a chemically-induced decondensation reaction.

Motile spermatozoa were used to ...


Translational Regulation Of The C-Jun Proto-Oncogene, Anil Sehgal Apr 1994

Translational Regulation Of The C-Jun Proto-Oncogene, Anil Sehgal

Theses and Dissertations in Biomedical Sciences

The v-jun oncogene was originally isolated from the ASV17 virus in 1987. Ever since its isolation, extensive work has been done to understand the role of the v-jun oncogene in cell transformation. The c-Jun protein is a transcription factor which binds to the DNA target TGACTCA. The c-Jun protein binds to DNA in the form of dimers. It can form homodimers with itself and heterodimers with Jun family (JunB and JunD), Fos family (FosB, Fra1 and Fra2), or with CREB family members through the leucine zipper motif. Because the c-jun proto-oncogene plays an important role in cell transformation, extensive work ...


Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi Jan 1994

Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi

Journal of the Arkansas Academy of Science

Liver lipid changes in male BALB-c nude mice due to subcutaneously implanted human prostate metastatic grade IV adenocarcinoma was studied. The prostate cancer cells were cultured in F12 plus 7.5% horse serum and 25% fetal calf serum medium. When they reached confluence, some of these cells were fixed with glutaraldehyde and thoroughly washed with buffer then 4 x107 cells were implanted into four mice. Four more mice were implanted with 4 x 106 viable, unfixed cells. Four uninjected mice served as controls. All the mice were sacrificed 18 days later. The total liver lipids (TLL) from each liver were ...


Correlation Between Chromatid Deletion Production And Progression Of The Dna Replication Fork In Uv-Irradiated S Phase Xenopus Cells, Daniel M. Yoder, Jason M. Hiles, H. Gaston Griggs Jan 1994

Correlation Between Chromatid Deletion Production And Progression Of The Dna Replication Fork In Uv-Irradiated S Phase Xenopus Cells, Daniel M. Yoder, Jason M. Hiles, H. Gaston Griggs

Journal of the Arkansas Academy of Science

Experimentation was performed primarily to determine whether progression of the DNA replication fork along segments of S phase Xenopus chromosomes, which contain UV-induced pre-aberrational lesions, plays a significant role in conversion of these lesions into chromatid deletions. Specifically, a Xenopus chromosome that was both easy to identify and that possessed a single DNA replication fork in one arm was found and used to conduct the experimentation. This chromosome was exposed to UV in early S phase and a Bromodeoxyuridine/Giemsa differential staining technique was applied in conjunction with conventional aberrational techniques to correlate progression of the DNA replication fork through ...


Examination Of The Effects Of Selected Factors On The Talin-Actin Interaction , Jean Marie Schmidt Jan 1994

Examination Of The Effects Of Selected Factors On The Talin-Actin Interaction , Jean Marie Schmidt

Retrospective Theses and Dissertations

Talin is an adhesion plaque protein believed to be involved in linking actin filaments to cell membranes. In these studies I examined the effects of selected factors on the ability of talin and its 190 kDa proteolytic fragment to interact with actin. A modified method for purifying talin from avian smooth muscle was devised. Cosedimentation assays showed that increasing amounts of talin bound to actin filaments as pH was decreased from 7.4 to 6.4. Talin's ability to increase viscosity of F-actin solutions also was increased with decreasing pH within this range. Electron microscope observations supported a pH-dependent ...


Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang Jan 1994

Molecular Mechanisms Of Mycoplasma Hyopneumoniae Adherence To Swine Respiratory Epithelial Cells, Qijing Zhang

Retrospective Theses and Dissertations

A microtiter plate adherence assay for Mycoplasma hyopneumoniae was established by using purified swine tracheal cilia which are the natural targets for the mycoplasma. M. hyopneumoniae bound specifically to solubilized cilia immobilized onto microtiter plates. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited binding of the mycoplasmas to cilia. Heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesins on the surface of mycoplasmas, whereas laminin blocked the receptors in cilia. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas; whereas, treatment of cilia with sodium metaperiodate decreased the binding. In the second study ...


Identification Of Potential Oat Phytochrome A Mrna Degradation Mechanisms , David Charles Higgs Jan 1994

Identification Of Potential Oat Phytochrome A Mrna Degradation Mechanisms , David Charles Higgs

Retrospective Theses and Dissertations

The phytochrome plant photoreceptor is important for perceiving the light environment and initiating plant developmental and environmental responses. When dark-grown oat seedlings are treated with light the phytochrome A (PHYA) mRNA abundance rapidly declines due, in part, to the inherent instability of the PHYA mRNA. Messenger RNA stability is an important process in regulating eukaryotic gene expression. My primary goal was to investigate both the mechanism of oat PHYA mRNA degradation and cis-acting sequences involved in regulating oat PHYA mRNA degradation. An oat protoplast system was established to investigate its use as a quick and reliable system to study plant ...


An Investigation Into The Role Of Arterial Wall Vibration In The Pathogenesis Of Atherosclerosis , Becky Bellatti Bittle Jan 1994

An Investigation Into The Role Of Arterial Wall Vibration In The Pathogenesis Of Atherosclerosis , Becky Bellatti Bittle

Retrospective Theses and Dissertations

Fifty percent of all the deaths in the Western world can be attributed to diseases of the cardiovascular system. A major player amongst these diseases is atherosclerosis. Atherosclerosis is the localized thickening of the arterial wall and is primarily due to the proliferation of fibrous tissue and smooth muscle cells in conjunction with the deposition of lipids within the wall. The hypothesis investigated was that high frequency, low amplitude vibratory environments similar to those found in regions of disturbed flow, which are coincident with intimal thickenings, the earliest stage of atherosclerosis, do elicit a proliferative response in vascular smooth muscle ...


Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer Jan 1994

Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer

Retrospective Theses and Dissertations

The budding yeast Saccharomyces cerevisiae is a dimorphic organism that can assume either a yeast-like or pseudohyphal form. Nitrogen limitation induces pseudohyphal growth, which is characterized by branched chains of elongated cells. Pseudohyphal cells can grow invasively in agar medium, whereas yeast-like cells do not. To identify factors involved in morphologic differentiation, S. cerevisiae mutants exhibiting a constitutive cell elongation morphology were isolated. Genetic analysis identified 28 recessive and 2 semi-dominant mutations that cause abnormal morphology, and placed these in 14 distinct gene loci, termed ELM, for ELongated Morphology. Many elm mutations cause multiple aspects of pseudohyphal growth, and thus ...


Neuroligand-Evoked Release Of Excitatory Neurotransmitters From Cultured Glial Cells , Fang Liu Jan 1994

Neuroligand-Evoked Release Of Excitatory Neurotransmitters From Cultured Glial Cells , Fang Liu

Retrospective Theses and Dissertations

Neuroglia have multiple roles in nervous system which include the regulation of external potassium, the uptake of excitatory amino acids and releasing neurotransmitters such as glutamate and aspartate. There are three different mechanisms proposed for the release of glutamate. One of them is the calcium dependency of glutamate release; The second one is reversal of uptake of glutamate; Furosemide-sensitive release of glutamate from swelling cells is the third proposed mechanism;The objectives of this research are to determine whether neuroligand bradykinin (BK) and adenosine 5'-triphosphate (ATP) can stimulate release of excitatory amino acid (EAA) glutamate and aspartate from cultured ...


Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen Jan 1994

Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen

Retrospective Theses and Dissertations

Mitogen regulated protein/proliferin (MRP/PLF) is a murine uterine growth factor belonging to the prolactin/growth hormone gene superfamily. Basic fibroblast growth factor (bFGF) regulates its expression in 3T3 cells and is speculated to be among its regulators in vivo. In order to gain insights into its regulation, experiments were performed to elucidate the mechanisms by which bFGF regulates mrp/plf gene expression in 3T3 cells. RT-PCR analyses demonstrated that of the three different forms of MRP/PLF proteins only one, PLF1, is expressed in these cells in response to bFGF. PLF1 could be the product of either plf42 ...


Nuclear Matrix Proteins Distinguish Normal Diploid Osteoblasts From Osteosarcoma Cells, Joseph P. Bidwell, Edward G. Fey, Andre J. Van Wijnen, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein Jan 1994

Nuclear Matrix Proteins Distinguish Normal Diploid Osteoblasts From Osteosarcoma Cells, Joseph P. Bidwell, Edward G. Fey, Andre J. Van Wijnen, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein

Open Access Articles

Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with those in normal diploid osteoblasts. The tumor-derived cells coexpress genes which are expressed in a sequential and mutually exclusive manner during the progressive stages of osteoblast differentiation. In osteosarcoma cells two-dimensional electrophoretic analysis indicates a composite representation of nuclear matrix proteins characteristic of both the proliferative and postproliferative periods of osteoblast phenotype development. In addition, nuclear matrix proteins unique to the tumor cells and the absence of nuclear matrix proteins found ...


Binding Of Matrix Attachment Regions To Lamin Polymers Involves Single-Stranded Regions And The Minor Groove., M. E. Eva Ludérus, Jan L. Den Blaauwen, Oncko J. De Smit, Duane A. Compton, Roel Van Driel Jan 1994

Binding Of Matrix Attachment Regions To Lamin Polymers Involves Single-Stranded Regions And The Minor Groove., M. E. Eva Ludérus, Jan L. Den Blaauwen, Oncko J. De Smit, Duane A. Compton, Roel Van Driel

Open Dartmouth: Faculty Open Access Scholarship

Chromatin in eukaryotic nuclei is thought to be partitioned into functional loop domains that are generated by the binding of defined DNA sequences, named MARs (matrix attachment regions), to the nuclear matrix. We have previously identified B-type lamins as MAR-binding matrix components (M. E. E. Ludérus, A. de Graaf, E. Mattia, J. L. den Blaauwen, M. A. Grande, L. de Jong, and R. van Driel, Cell 70:949-959, 1992). Here we show that A-type lamins and the structurally related proteins desmin and NuMA also specifically bind MARs in vitro. We studied the interaction between MARs and lamin polymers in molecular ...


Cell Division In The Multicellular Marine Red Alga Agardhiella Subulata, Karel Joan Klepacki Jan 1994

Cell Division In The Multicellular Marine Red Alga Agardhiella Subulata, Karel Joan Klepacki

Dissertations, Theses, and Masters Projects

No abstract provided.


The Effects Of Contaminated Sediment On The Epidermal Goblet Cells Of The Mummichog, Fundulus Heteroclitus, Laurent C. Mézin Jan 1994

The Effects Of Contaminated Sediment On The Epidermal Goblet Cells Of The Mummichog, Fundulus Heteroclitus, Laurent C. Mézin

Dissertations, Theses, and Masters Projects

Secretion of mucus by epidermal goblet cells is an important first line of defense for teleosts. It protects them against many of the biological, physical and chemical insults they encounter in their environment. This project monitored changes in hemoglobin concentration in epidermal mucus and in the density, diameter and mucus quality of epidermal goblet cells in the mummichog, Fundulus heteroclitus, following exposure to creosote-contaminated sediment. Male fish (200) were exposed for 13 days in flowthough aquaria to either an uncontaminated reference sediment or contaminated sediment containing 30% Elizabeth River sediment. Fish were sampled on Days 0, 3, 7 and 13 ...