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Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1991

Antigens; Cell Nucleus; Cells, Cultured; Fluorescent Antibody Technique; Humans; Male; Mitosis; Poly A; RNA; Ribonucleoproteins; Ribonucleoproteins, Small Nuclear; Transcription, Genetic

Articles 1 - 1 of 1

Full-Text Articles in Cell Biology

Discrete Nuclear Domains Of Poly(A) Rna And Their Relationship To The Functional Organization Of The Nucleus, Kenneth C. Carter, Krishan L. Taneja, Jeanne B. Lawrence Dec 1991

Discrete Nuclear Domains Of Poly(A) Rna And Their Relationship To The Functional Organization Of The Nucleus, Kenneth C. Carter, Krishan L. Taneja, Jeanne B. Lawrence

Open Access Articles

The functional organization of the nucleus was studied using a fluorescence microscopy approach which allowed integration of positional information for RNA, DNA, and proteins. In cells from sea urchin to human, nuclear poly(A) RNA was found concentrated primarily within several discrete "transcript domains" which often surrounded nucleoli. Concentrations of poly(A) RNA were coincident with snRNP antigen clusters, providing evidence for the localization of pre-mRNA splicing at these sites. The spatial relationship of transcript domains with respect to various classes of DNA was established, in that the poly(A) RNA-rich regions coincided with discrete regions of low DNA density ...