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Cell Biology Commons

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1991

GSBS Student Publications

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Full-Text Articles in Cell Biology

Preservation Of Specific Rna Distribution Within The Chromatin-Depleted Nuclear Substructure Demonstrated By In Situ Hybridization Coupled With Biochemical Fractionation, Yigong P. Xing, Jeanne B. Lawrence Mar 1991

Preservation Of Specific Rna Distribution Within The Chromatin-Depleted Nuclear Substructure Demonstrated By In Situ Hybridization Coupled With Biochemical Fractionation, Yigong P. Xing, Jeanne B. Lawrence

GSBS Student Publications

Biochemical fractionation procedures previously shown to remove 95% of cellular protein, DNA, and phospholipid, were combined with fluorescence in situ hybridization to provide a critical evaluation of the retention and spatial preservation of specific primary transcripts within the chromatin-depleted nuclear substructure, operationally defined as the nuclear "matrix." This unique approach made it possible to directly address whether nuclear extraction procedures preserve, create, or destroy ribonucleoprotein filament structures. Comparison of nuclei before and after fractionation demonstrated that localized foci or "tracks" of specific nRNA are unambiguously retained in the nuclear matrix preparation. Two well-characterized nuclear fractionation procedures were used and three ...