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1991

Anatomy

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Full-Text Articles in Cell Biology

In Vitro Desmin Assembly And In Vivo Changes In Developing Smooth Muscle , Rong-Ghi Robert Chou Jan 1991

In Vitro Desmin Assembly And In Vivo Changes In Developing Smooth Muscle , Rong-Ghi Robert Chou

Retrospective Theses and Dissertations

This dissertation is divided into two principal parts. The purpose of the first part was to investigate the effects of cations and temperature on the critical concentration of desmin required for filament assembly by using tow independent assays, namely, turbidity and centrifugation. Assembly was done in the presence of 2 mM Ca[superscript]2+, 2 mM Mg[superscript]2+ or 150 mM Na[superscript]+ at 2, 22 and 37°C. Similar values for critical concentration were obtained by both assays. As temperature increased, critical concentration decreased for each cation. Critical concentration was lowest in the presence of Ca[superscript]2 ...