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1984

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Articles 1 - 16 of 16

Full-Text Articles in Cell Biology

A Quantitative Study Of The Distinctive Proteins Of The Eosinophil: A Comparison Of Normals And Eosinophilic Patients, James M. Olson Dec 1984

A Quantitative Study Of The Distinctive Proteins Of The Eosinophil: A Comparison Of Normals And Eosinophilic Patients, James M. Olson

Honors Theses

Eosinophils have long been associated with parasitic and allergic diseases. Methods of procurement and purification of eosinophils in the last ten years have allowed intensive investigation into their components, properties, functions, and mechanisms (Gleich et al, 1982). The goals of this project are to separate eosinophils in blood samples taken from patients with eosinophilia and from normals based on density over distontinuous Metrizamide gradients; to determine quantitatively the levels of the three distinctive proteins of the eosinophil, specifically MBP, EDN, and ECP; and to compare the levels of these proteins found in eosinophils of patients with eosinophilia to the amounts ...


Glycolipids Of The Mouse Peritoneal Macrophage. Alterations In Amount And Surface Exposure Of Specific Glycolipid Species Occur In Response To Inflammation And Tumoricidal Activation, Arthur M. Mercurio, Gerald A. Schwarting, Phillips W. Robbins Oct 1984

Glycolipids Of The Mouse Peritoneal Macrophage. Alterations In Amount And Surface Exposure Of Specific Glycolipid Species Occur In Response To Inflammation And Tumoricidal Activation, Arthur M. Mercurio, Gerald A. Schwarting, Phillips W. Robbins

Cancer Biology Publications and Presentations

We have characterized the major glycolipid constituents of the mouse peritoneal macrophage, and have demonstrated that alterations in the amount and in the accessibility of specific glycolipid species to galactose oxidase/NaB3H4 labeling, an indicator of glycolipid surface exposure, occur in response to inflammation and as a consequence of activation to a tumoricidal state. The key findings are: (a) Asialo GM1, a major neutral glycolipid constituent of all macrophage populations examined, is accessible to galactose oxidase/NaB3H4 labeling on the surface of TG-elicited and BCG-activated macrophages but not on resident macrophages; (b) GM1 is the predominant ganglioside constituent of the ...


Effect Of Adenovirus Infection On Expression Of Human Histone Genes, S. J. Flint, M. A. Plumb, U. C. Yang, Gary S. Stein, Janet L. Stein Jul 1984

Effect Of Adenovirus Infection On Expression Of Human Histone Genes, S. J. Flint, M. A. Plumb, U. C. Yang, Gary S. Stein, Janet L. Stein

Stein, Stein, Lian, vanWijnen Lab Publications

The influence of adenovirus type 2 infection of HeLa cells upon expression of human histone genes was examined as a function of the period of infection. Histone RNA synthesis was assayed after run-off transcription in nuclei isolated from mock-infected cells and after various periods of adenovirus infection. Histone protein synthesis was measured by [3H]leucine labeling of intact cells and fluorography of electrophoretically fractionated nuclear and cytoplasmic proteins. The cellular representation of RNA species complementary to more than 13 different human histone genes was determined by RNA blot analysis of total cellular, nuclear or cytoplasmic RNA by using a series ...


A Membrane Cytoskeleton From Dictyostelium Discoideum. Ii. Integral Proteins Mediate The Binding Of Plasma Membranes To F-Actin Affinity Beads, Elizabeth J. Luna, C. M. Goodloe-Holland, H. M. Ingalls Jul 1984

A Membrane Cytoskeleton From Dictyostelium Discoideum. Ii. Integral Proteins Mediate The Binding Of Plasma Membranes To F-Actin Affinity Beads, Elizabeth J. Luna, C. M. Goodloe-Holland, H. M. Ingalls

Women’s Health Research Faculty Publications

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to F-actin beads (fluorescein-labeled F-actin on antifluorescein IgG-Sephacryl S-1000 beads). Binding was found to be (a) specific, since beads containing bound fluorescein-labeled ovalbumin or beads without bound fluorescein-labeled protein do not bind membranes, (b) saturable at approximately 0.6 microgram of membrane protein per microgram of bead-bound F-actin, (c) rapid with a t1/2 of 4-20 min, and (d) apparently of reasonable affinity since the off rate is too slow to be measured by present techniques. Using low-speed sedimentation assays, we found that sonicated plasma membrane ...


A Membrane Cytoskeleton From Dictyostelium Discoideum. Iii. Plasma Membrane Fragments Bind Predominantly To The Sides Of Actin Filaments, C. M. Goodloe-Holland, Elizabeth J. Luna Jul 1984

A Membrane Cytoskeleton From Dictyostelium Discoideum. Iii. Plasma Membrane Fragments Bind Predominantly To The Sides Of Actin Filaments, C. M. Goodloe-Holland, Elizabeth J. Luna

Women’s Health Research Faculty Publications

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads was found to be competitively inhibited by myosin subfragment-1. This inhibition is MgATP-sensitive, exhibits a Ki of approximately 5 X 10(-8) M, and is reciprocal, since membranes inhibit the binding of 125I-heavy meromyosin to F-actin on beads. These experiments demonstrate that membrane binding and S-1 binding to F-actin on beads are mutually exclusive and, therefore, that the membrane fragments bind predominantly to the sides, rather than to the ends, of the actin filaments. This conclusion is supported by electron micrographs that show many lateral ...


Hemoglobin Synthesis In Mice Doubly Heterozygous For Alpha And Beta Thalassemia, Eric Smith Apr 1984

Hemoglobin Synthesis In Mice Doubly Heterozygous For Alpha And Beta Thalassemia, Eric Smith

Life and Environmental Sciences Undergraduate Theses

The extent of imbalance between the synthesis rate of «-globin polypeptide chains relative to B-globin chains in thalassemic mice is one of the major factors in determining the pathophysiology of thalassemia. Mice doubly heterozygous for both a mutant ®<-thalassemic chromosome and a /?-thalassemic chromosome exhibited a hypochromic, microcytic anemia with mild reticulocytosis and erythrocytosis. However, the erythrocytes of these mice displayed few signs of hemolysis, or poikilocytosis, and did not contain visible inclusion bodies. Analysis of the relative rates of globin chain syntheses in vitro by ^H-leucine incorporation revealed the


A Study Of The Binding Of Rna Polymerase Ii To Mononucleosomes, Sean Russell Apr 1984

A Study Of The Binding Of Rna Polymerase Ii To Mononucleosomes, Sean Russell

Life and Environmental Sciences Undergraduate Theses

In order to arrive at a basic understanding of the mechanism of gene expression in eukaryotic cells, one must first understand the structure and interaction of their components: the structure and conformation of DNA in active and inactive genes, the modification of histones, and the interaction of factors during transcription. Increased research has resulted in several important observations, such as that of the subunit of chromatin structure.^ Further research is underway to determine if this subunit of structure is the actual conformation of DNA in active genes, 2 and how it might be affected by histone modification. Ongoing efforts have ...


The Use Of Envelope Specific Monoclonal Antibodies To Search For Differentiation Antigens Encoded By Endogenous Retroviral Sequences In Murine Hematopoietic Cells, David Olson Apr 1984

The Use Of Envelope Specific Monoclonal Antibodies To Search For Differentiation Antigens Encoded By Endogenous Retroviral Sequences In Murine Hematopoietic Cells, David Olson

Life and Environmental Sciences Undergraduate Theses

Hematopoietic cells in the mouse were examined to detect retrovirus encoded differentiation antigens on their cell surfaces. Twenty-four established lymphoid, erythroid, and myeloid cell lines were studied. A panel of well defined envelope specific monoclonal antibodies were tested against 125 each cell line in a I-protein A cell binding radioimmunoassay to detect retroviral envelope antigens on the cell's plasma membrane. Results were analyzed to discern cell line specific expression of antigens as a basis for the search for differentiation antigens. A few cell lines were selected for further study. Two erythroid cell lines, 7727 and 7883, for example, were ...


Preliminary Characterization Of The P3 Human Epithelial Cell Line For Radiobiological Reseach, John Holland Apr 1984

Preliminary Characterization Of The P3 Human Epithelial Cell Line For Radiobiological Reseach, John Holland

Life and Environmental Sciences Undergraduate Theses

Preliminary experimentation was performed to characterize the P3 human epithelial cell line for radiobiological research. Specifically, work was done to determine if the cell line should be used to evaluate mechanisms of radiation damage and repair as revealed by survival response experimentation and via the 6-thioguanine mutation assay. Growth analysis was performed as well as a 6-thioguanine toxicity study and a 6-thioguanine mutation expression time curve. Preliminary results were obtained from survival experiments following P3 cell irradiation with 50 KvP X-rays, 6^co gamma rays, and Janus Reactor fission spectrum neutrons with a mean energy of 0.86 MeV. These ...


The Cytotoxic And Kinetic Effects Of Asynchronous Cells Following The Administration Of Vincristine, Meri Firpo Apr 1984

The Cytotoxic And Kinetic Effects Of Asynchronous Cells Following The Administration Of Vincristine, Meri Firpo

Life and Environmental Sciences Undergraduate Theses

A growth curve was constructed for SCCVII-SF, a mouse squamous cell carcinoma cell line, in a culture of modified q-crle's medium. A toxicity curve was constructed for vincristine ,nd SCCVII-SF cells in the exponential phase of growth. Cells in mid-exponential phase were treated with a subtoxic level of vincristine for four hours. A count of mitotic cells was taken at intervals to determine whether vincristine caused a buildup of cells in mitosis after its removal. Results showed that cells continued to collect in mitosis for four hours after the removal of vincristine.


Purification And Characterization Of A Camp- And Ca2+-Calmodulin-Independent Glycogen Synthase Kinase From Porcine Renal Cortex, Stephen J. Beebe, Erwin M. Reimann, Keith K. Schlender Feb 1984

Purification And Characterization Of A Camp- And Ca2+-Calmodulin-Independent Glycogen Synthase Kinase From Porcine Renal Cortex, Stephen J. Beebe, Erwin M. Reimann, Keith K. Schlender

Bioelectrics Publications

We recently reported the partial purification of a cAMP-independent and Ca2+-calmodulin-independent glycogen synthase kinase from porcine renal cortex (Schlender, K. K., Beebe, S. J., and Reimann, E. M. (1981) Cold Spring Harbor Conf. Cell Proliferation, 389-400). Subsequent purification indicated that the enzyme preparation consisted of at least three forms of glycogen synthase kinase which could be resolved by ATP gradient elution from aminoethylphosphate-agarose (AEP-agarose). The predominant form of glycogen synthase kinase, which eluted from AEP-agarose between 2 and 6 mM ATP, was purified approximately 800-fold and is designated GSK-A1. It had a molecular weight of 45,000-50,000 ...


Effects Of Cannabinoids On Gene Expression, Gary S. Stein, Janet L. Stein Jan 1984

Effects Of Cannabinoids On Gene Expression, Gary S. Stein, Janet L. Stein

Stein, Stein, Lian, vanWijnen Lab Publications

No abstract provided.


Microheterogeneity Of Type Ii Camp-Dependent Protein Kinase In Various Mammalian Species And Tissues, Alison M. Robinson-Steiner, Stephen J. Beebe, Stephen R. Rannels, Jackie D. Corbin Jan 1984

Microheterogeneity Of Type Ii Camp-Dependent Protein Kinase In Various Mammalian Species And Tissues, Alison M. Robinson-Steiner, Stephen J. Beebe, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Excluding autophosphorylated species, at least six forms of the regulatory subunit of type II cAMP-dependent protein kinase (R(II)) from various mammalian tissues were identified by sodium dodecyl sulfate (SDS) gel electrophoresis of purified samples and of crude preparations photoaffinity labeled with 8-azido[32P] cAMP and by gel filtration. After autophosphorylation some heart R(II) forms termed type IIA (bovine, porcine, equine, and dog) shifted to a more slowly migrating band on SDS gels while others termed type IIB (rat, guinea pig, rabbit, and monkey) did not detectably shift. Both subclasses of R(II) exhibited variation in apparent M(r ...


Two Classes Of Camp Analogs Which Are Selective For The Two Different Camp-Binding Sites Of Type Ii Protein Kinase Demonstrate Synergism When Added Together To Intact Adipocytes, Stephen J. Beebe, Rob Holloway, Stephen R. Rannels, Jackie D. Corbin Jan 1984

Two Classes Of Camp Analogs Which Are Selective For The Two Different Camp-Binding Sites Of Type Ii Protein Kinase Demonstrate Synergism When Added Together To Intact Adipocytes, Stephen J. Beebe, Rob Holloway, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Twenty-five cyclic nucleotide analogs were tested individually to act as lipolytic agents and to activate adipocyte protein kinase. The lipolytic potency of individual analogs correlated better with their K(a) for protein kinase and their lipophilicity rather than with either parameters alone. Some of the most potent lipolytic analogs had high I50 values for the particulate low K(m) cAMP phosphodiesterase suggesting that their effect was not due to raising endogenous cAMP levels through inhibition of phosphodiesterase. The most potent lipolytic analogs contained a thio moiety at the C-8 or C-6 position. These analogs exhibited concave upward dose-response curves. At ...


Inquiry Into The Causes And Significance Of Cytoplasmic Vacuolation Of Neutrophils In The Peripheral Circulation, Gary Xavier Haight Jan 1984

Inquiry Into The Causes And Significance Of Cytoplasmic Vacuolation Of Neutrophils In The Peripheral Circulation, Gary Xavier Haight

Dissertations and Theses

One-half million cases of septicemia are reported annually with a mortality of around 35%. Diagnosis depends in part on blood cultures which require one to two weeks. It would be advantageous if an early sign of septicemia were available.

Cytoplasmic vacuolation of polymorphonuclear neutropnils is occasionally seen in the peripheral blood smears of patients who have infections. The object of this work was to determine the cause and significance of cytoplasmic vacuolation with the goal of using the occurrence of vacuolation as an early indicator of infection.


The Ultrastructure Of Cell Division In The Marine Red Alga Lomentaria Baileyana, Elizabeth Carpenter Davis Jan 1984

The Ultrastructure Of Cell Division In The Marine Red Alga Lomentaria Baileyana, Elizabeth Carpenter Davis

Dissertations, Theses, and Masters Projects

No abstract provided.