Open Access. Powered by Scholars. Published by Universities.®

Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

University of Massachusetts Medical School Faculty Publications

2015

Articles 1 - 1 of 1

Full-Text Articles in Cell Biology

Probability-Based Particle Detection That Enables Threshold-Free And Robust In Vivo Single-Molecule Tracking, Carlas Smith, Sjoerd Stallinga, Keith A. Lidke, Bernd Rieger, David Grunwald Nov 2015

Probability-Based Particle Detection That Enables Threshold-Free And Robust In Vivo Single-Molecule Tracking, Carlas Smith, Sjoerd Stallinga, Keith A. Lidke, Bernd Rieger, David Grunwald

University of Massachusetts Medical School Faculty Publications

Single-molecule detection in fluorescence nanoscopy has become a powerful tool in cell biology but can present vexing issues in image analysis, such as limited signal, unspecific background, empirically set thresholds, image filtering, and false-positive detection limiting overall detection efficiency. Here we present a framework in which expert knowledge and parameter tweaking are replaced with a probability-based hypothesis test. Our method delivers robust and threshold-free signal detection with a defined error estimate and improved detection of weaker signals. The probability value has consequences for downstream data analysis, such as weighing a series of detections and corresponding probabilities, Bayesian propagation of probability ...