Open Access. Powered by Scholars. Published by Universities.®

Cell Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Cell Biology

Methods For High-Dimensonal Analysis Of Cells Dissociated From Cyropreserved Synovial Tissue, Laura T. Donlin, Ellen M. Gravallese, Jennifer H. Anolik Jul 2018

Methods For High-Dimensonal Analysis Of Cells Dissociated From Cyropreserved Synovial Tissue, Laura T. Donlin, Ellen M. Gravallese, Jennifer H. Anolik

Open Access Articles

BACKGROUND: Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership RA/SLE Network aims to deconstruct autoimmune pathology by examining cells within target tissues through multiple high-dimensional assays. Robust standardized protocols need to be developed before cellular phenotypes at a single cell level can be effectively compared across patient samples.

METHODS: Multiple clinical sites collected cryopreserved synovial tissue fragments from arthroplasty and synovial biopsy in a 10% DMSO solution. Mechanical and enzymatic dissociation parameters were optimized for viable cell extraction and surface ...


Plasma Circulating Extracellular Rnas In Left Ventricular Remodeling Post-Myocardial Infarction, Kirsty M. Danielson, Kahraman Tanriverdi, Jane E. Freedman, Saumya Das Jun 2018

Plasma Circulating Extracellular Rnas In Left Ventricular Remodeling Post-Myocardial Infarction, Kirsty M. Danielson, Kahraman Tanriverdi, Jane E. Freedman, Saumya Das

Open Access Articles

Despite substantial declines in mortality following myocardial infarction (MI), subsequent left ventricular remodeling (LVRm) remains a significant long-term complication. Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. RNA sequencing of exRNAs was performed on plasma samples from patients with "beneficial" (decrease LVESVI > /=20%, n=11) and "adverse" (increase LVESVI > /=15%, n=11) LVRm. Selected differentially expressed exRNAs were validated by RT-qPCR (n=331) and analyzed for their association with LVRm determined by cardiac MRI. Principal components of exRNAs were associated with LVRm phenotypes post-MI ...


Meeting Report: Discussions And Preliminary Findings On Extracellular Rna Measurement Methods From Laboratories In The Nih Extracellular Rna Communication Consortium, Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, Jorge Arango, Leonora Balaj, Xandra Breakefield, Elizabeth Carlson, Bob S. Carter, Blanca Majem, Clark C. Chen, Emanuele Cocucci, Kirsty Danielson, Amanda Courtright, Saumya Das, Zakaria Y. Abd Elmageed, Daniel Enderle, Alan Ezrin, Marc Ferrer, Jane Freedman, Kahraman Tanriverdi Aug 2015

Meeting Report: Discussions And Preliminary Findings On Extracellular Rna Measurement Methods From Laboratories In The Nih Extracellular Rna Communication Consortium, Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, Jorge Arango, Leonora Balaj, Xandra Breakefield, Elizabeth Carlson, Bob S. Carter, Blanca Majem, Clark C. Chen, Emanuele Cocucci, Kirsty Danielson, Amanda Courtright, Saumya Das, Zakaria Y. Abd Elmageed, Daniel Enderle, Alan Ezrin, Marc Ferrer, Jane Freedman, Kahraman Tanriverdi

Open Access Articles

Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in exRNAs lies in the fact that they may serve as signalling molecules between cells, their potential to serve as biomarkers for prediction and diagnosis of disease and the possibility that exRNAs or the extracellular particles that carry them might be used for therapeutic purposes. Among the most significant bottlenecks to progress in this field is the lack of robust and standardized methods for collection and processing of biofluids, separation of ...