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Articles 1 - 4 of 4

Full-Text Articles in Cell Biology

Extracellular-Signal Regulated Kinase: A Central Molecule Driving Epithelial-Mesenchymal Transition In Cancer, Monserrat Olea-Flores, Miriam Daniela Zuniga-Eulogio, Miguel Angel Mendoza-Catalan, Hugo Alberto Rodriguez-Ruiz, Eduardo Castaneda-Saucedo, Carlos Ortuno-Pineda, Teresita Padilla-Benavides, Napoleon Navarro-Tito Jun 2019

Extracellular-Signal Regulated Kinase: A Central Molecule Driving Epithelial-Mesenchymal Transition In Cancer, Monserrat Olea-Flores, Miriam Daniela Zuniga-Eulogio, Miguel Angel Mendoza-Catalan, Hugo Alberto Rodriguez-Ruiz, Eduardo Castaneda-Saucedo, Carlos Ortuno-Pineda, Teresita Padilla-Benavides, Napoleon Navarro-Tito

Open Access Articles

Epithelial-mesenchymal transition (EMT) is a reversible cellular process, characterized by changes in gene expression and activation of proteins, favoring the trans-differentiation of the epithelial phenotype to a mesenchymal phenotype. This process increases cell migration and invasion of tumor cells, progression of the cell cycle, and resistance to apoptosis and chemotherapy, all of which support tumor progression. One of the signaling pathways involved in tumor progression is the MAPK pathway. Within this family, the ERK subfamily of proteins is known for its contributions to EMT. The ERK subfamily is divided into typical (ERK 1/2/5), and atypical (ERK 3/4 ...


Ligand-Activated Bmp Signaling Inhibits Cell Differentiation And Death To Promote Melanoma, Arvind Venkatesan, Rajesh Vyas, Alec Gramann, Karen A. Dresser, Sharvari Gujja, Sanchita Bhatnagar, Sagar Chhangawala, Camilla Borges Ferreira Gomes, Hualin Simon Xi, Christine G. Lian, Yariv Houvras, Yvonne J. K. Edwards, April C. Deng, Michael R. Green, Craig J. Ceol Jan 2018

Ligand-Activated Bmp Signaling Inhibits Cell Differentiation And Death To Promote Melanoma, Arvind Venkatesan, Rajesh Vyas, Alec Gramann, Karen A. Dresser, Sharvari Gujja, Sanchita Bhatnagar, Sagar Chhangawala, Camilla Borges Ferreira Gomes, Hualin Simon Xi, Christine G. Lian, Yariv Houvras, Yvonne J. K. Edwards, April C. Deng, Michael R. Green, Craig J. Ceol

Open Access Articles

Oncogenomic studies indicate that copy number variation (CNV) alters genes involved in tumor progression; however, identification of specific driver genes affected by CNV has been difficult, as these rearrangements are often contained in large chromosomal intervals among several bystander genes. Here, we addressed this problem and identified a CNV-targeted oncogene by performing comparative oncogenomics of human and zebrafish melanomas. We determined that the gene encoding growth differentiation factor 6 (GDF6), which is the ligand for the BMP family, is recurrently amplified and transcriptionally upregulated in melanoma. GDF6-induced BMP signaling maintained a trunk neural crest gene signature in melanomas. Additionally, GDF6 ...


High-Resolution Proteomic And Lipidomic Analysis Of Exosomes And Microvesicles From Different Cell Sources, Reka A. Haraszti, Marie-Cecile Didiot, Ellen Sapp, John D. Leszyk, Scott A. Shaffer, Hannah E. Rockwell, Fei Gao, Niven R. Narain, Marian Difiglia, Michael A. Kiebish, Neil Aronin, Anastasia Khvorova Nov 2016

High-Resolution Proteomic And Lipidomic Analysis Of Exosomes And Microvesicles From Different Cell Sources, Reka A. Haraszti, Marie-Cecile Didiot, Ellen Sapp, John D. Leszyk, Scott A. Shaffer, Hannah E. Rockwell, Fei Gao, Niven R. Narain, Marian Difiglia, Michael A. Kiebish, Neil Aronin, Anastasia Khvorova

Open Access Articles

Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were ...


Border Security: The Role Of Ripk3 In Epithelium Homeostasis, Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Chan Jun 2016

Border Security: The Role Of Ripk3 In Epithelium Homeostasis, Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Chan

Open Access Articles

Receptor interacting protein kinase 3 (RIPK3) is a crucial inducer of necroptosis. Its activity is controlled by interaction with other signal adaptors through the "RIP homotypic interaction motif" (RHIM). Recent studies revealed a critical function for RIPK3 in the maintenance of epithelial tissue integrity. In mice with genetic deficiency of the apoptosis adaptors FADD or caspase 8, RIPK3 promotes necroptotic cell death of epithelial cells, leading to excessive and lethal inflammation. In contrast, when FADD and caspase 8 functions are intact, RIPK3 serves as a protector of intestinal epithelial integrity by promoting injury-induced wound repair. In the latter case, RIPK3 ...