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Open Access Articles

1994

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Articles 1 - 5 of 5

Full-Text Articles in Cell Biology

Sequences Responsible For Intracellular Localization Of Beta-Actin Messenger Rna Also Affect Cell Phenotype, Edward H. Kislauskis, Xiaochun Zhu, Robert H. Singer Oct 1994

Sequences Responsible For Intracellular Localization Of Beta-Actin Messenger Rna Also Affect Cell Phenotype, Edward H. Kislauskis, Xiaochun Zhu, Robert H. Singer

Open Access Articles

We have characterized the structure and function of RNA sequences that direct beta-cytoplasmic actin mRNA to the cell periphery were mapped to two segments of 3'-untranslated region by expression of LacZ/beta-actin chimeric mRNAs in chicken embryo fibroblasts (CEFs). A 54-nt segment, the "RNA zipcode," and a homologous but less active 43-nt segment each localized beta-galactosidase activity to the leading lamellae. This zipcode contains the full activity, and mutations or deletions within it reduce, but do not eliminate, its activity, indicating that several motifs contribute to the activity. Two of these motifs, when multimerized, can regenerate almost full activity ...


Mediation Of Chemoattractant-Induced Changes In [Ca2+]I And Cell Shape, Polarity, And Locomotion By Insp3, Dag, And Protein Kinase C In Newt Eosinophils, Susan H. Gilbert, Kristine Perry, Fredric S. Fay Oct 1994

Mediation Of Chemoattractant-Induced Changes In [Ca2+]I And Cell Shape, Polarity, And Locomotion By Insp3, Dag, And Protein Kinase C In Newt Eosinophils, Susan H. Gilbert, Kristine Perry, Fredric S. Fay

Open Access Articles

During chemotaxis large eosinophils from newts exhibit a gradient of [Ca2+]i from rear to front. The direction of the gradient changes on relocation of the chemoattractant source, suggesting that the Ca2+ signal may trigger the cytoskeletal reorganization required for cell reorientation during chemotaxis. The initial stimulatory effect of chemoattractant on [Ca2+]i and the opposite orientations of the intracellular Ca2+ gradient and the external stimulus gradient suggest that more than one chemoattractant-sensitive messenger pathway may be responsible for the generation of spatially graded Ca2+ signals. To identify these messengers, Ca2+ changes were measured in single live cells stimulated with ...


A Double Leucine Within The Glut4 Glucose Transporter Cooh-Terminal Domain Functions As An Endocytosis Signal, Silvia Corvera, Anil Chawla, Ranjan Chakrabarti, Marguerite Joly, Joanne M. Buxton, Michael P. Czech Aug 1994

A Double Leucine Within The Glut4 Glucose Transporter Cooh-Terminal Domain Functions As An Endocytosis Signal, Silvia Corvera, Anil Chawla, Ranjan Chakrabarti, Marguerite Joly, Joanne M. Buxton, Michael P. Czech

Open Access Articles

The unique COOH-terminal 30-amino acid region of the adipocyte/skeletal muscle glucose transporter (GLUT4) appears to be a major structural determinant of this protein's perinuclear localization, from where it is redistributed to the cell surface in response to insulin. To test whether an underlying mechanism of this domain's function involves glucose transporter endocytosis rates, transfected cells were generated expressing exofacial hemagglutinin epitope (HA)-tagged erythrocyte/brain glucose transporter (GLUT1) or a chimera containing the COOH-terminal 30 amino acids of GLUT4 substituted onto this GLUT1 construct. Incubation of COS-7 or CHO cells expressing the HA-tagged chimera with anti-HA antibody ...


Dynamic Changes In The Higher-Level Chromatin Organization Of Specific Sequences Revealed By In Situ Hybridization To Nuclear Halos, Michael G. Gerdes, Kenneth C. Carter, Phillip T. Moen, Jeanne B. Lawrence Jul 1994

Dynamic Changes In The Higher-Level Chromatin Organization Of Specific Sequences Revealed By In Situ Hybridization To Nuclear Halos, Michael G. Gerdes, Kenneth C. Carter, Phillip T. Moen, Jeanne B. Lawrence

Open Access Articles

A novel approach to study the higher level packaging of specific DNA sequences has been developed by coupling high-resolution fluorescence hybridization with biochemical fractionation to remove histones and distend DNA loops to form morphologically reproducible nuclear "halos." Results demonstrate consistent differences in the organization of specific sequences, and further suggest a relationship to functional activity. Pulse-incorporated bromodeoxyuridine representing nascent replicating DNA localized with the base of the chromatin loops in discrete clustered patterns characteristic of intact cells, whereas at increasing chase times, the replicated DNA was consistently found further out on the extended region of the halo. Fluorescence hybridization to ...


Nuclear Matrix Proteins Distinguish Normal Diploid Osteoblasts From Osteosarcoma Cells, Joseph P. Bidwell, Edward G. Fey, Andre J. Van Wijnen, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein Jan 1994

Nuclear Matrix Proteins Distinguish Normal Diploid Osteoblasts From Osteosarcoma Cells, Joseph P. Bidwell, Edward G. Fey, Andre J. Van Wijnen, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein

Open Access Articles

Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with those in normal diploid osteoblasts. The tumor-derived cells coexpress genes which are expressed in a sequential and mutually exclusive manner during the progressive stages of osteoblast differentiation. In osteosarcoma cells two-dimensional electrophoretic analysis indicates a composite representation of nuclear matrix proteins characteristic of both the proliferative and postproliferative periods of osteoblast phenotype development. In addition, nuclear matrix proteins unique to the tumor cells and the absence of nuclear matrix proteins found ...