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Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Molecular Genetics

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Dissecting Membrane Trafficking In The Genomic Era: Components And Molecular Mechanisms, Eric Michael Davis Jan 2015

Dissecting Membrane Trafficking In The Genomic Era: Components And Molecular Mechanisms, Eric Michael Davis

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Forward genetics in classic model organisms has been a key driving force for establishing the basic principles of cellular biology. However, many cellular pathways including membrane trafficking pathways are more complex in mammals, often exhibiting unique features not found in lower model organisms. To address this, I use a multi-pronged approach taking advantage of the haploid genetic system in human cells to explore these genetic features in a mammalian cell context. First, I direct a series of forward comparative genetic screens toward the dissection of a class of membrane bound molecules -the glycophosphatidylinositol-anchored proteins (GPI- APs). My findings demonstrate that ...