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Articles 1 - 30 of 79

Full-Text Articles in Cell Biology

Principles And Properties Of Rna Self-Assembly, Gabriel Tauber Jan 2019

Principles And Properties Of Rna Self-Assembly, Gabriel Tauber

Undergraduate Honors Theses

Ribonucleoprotein (RNP) granules are membraneless assemblies of condensed RNA and protein that form through multivalent interactions between the constituent components. Recently, RNA self-assembly has been shown to contribute to the formation of stress granules, which are RNP granules associated with the stress response and neurodegeneration. However, little is understood about the properties of self-assembled RNA. Here, I show that different RNA sequences can self- assemble into condensates with distinct material properties and partitioning preferences in vitro. Due to interactions that minimize surface free energy, RNA self-assembly can generate the spontaneous self-patterning of RNA assemblies. By reducing surface free energy, thereby ...


Predicting Persisters: Investigating Gene Regulation In Persister Cells, Sowmya Dhanwada Jan 2019

Predicting Persisters: Investigating Gene Regulation In Persister Cells, Sowmya Dhanwada

Undergraduate Honors Theses

Bacterial populations produce a small number of persister cells that remain tolerant to multidrug exposure by becoming metabolically dormant. This inquiry focuses on investigating metabolic gene expression levels in persisters to determine potential predictors of bacterial persistence. Strains from the E. Coli Promoter library were imaged using single cell microscopy, and gene expression levels were measured with the integrated reporter GFP. Persister cells were isolated using ofloxacin. Results indicate that bacterial cells that survive antibiotic exposure exhibit filamentous growth before dividing. Resumption of normal growth morphology is accompanied by increased proteolytic activity in daughter cells, however more investigation is required ...


Developing Novel Methods To Investigate Real-Time In Vivo Dopamine Dynamics In The Monogamous Prairie Vole, Emma Burt Jan 2019

Developing Novel Methods To Investigate Real-Time In Vivo Dopamine Dynamics In The Monogamous Prairie Vole, Emma Burt

Undergraduate Honors Theses

Humans are among a small percentage of mammals that form monogamous relationships. These relationships, along with other strong social bonds, are essential to human well-being. In the absence of strong social relationships, humans experience a variety of detrimental conditions such as depression, anxiety, and cardiovascular disease. Traditional barriers to studying social bonds stem from the use of lab rodents such as mice and rats which do not form selective social attachments. However, the prairie vole (Microtus ochrogaster) does form these selective social attachments. The robust social bonds – pair bonds – formed between adult prairie voles may be observed and therefore studied ...


Identification And Characterization Of Regulators Of Glut4 Trafficking, Daniel Richard Gulbranson Feb 2018

Identification And Characterization Of Regulators Of Glut4 Trafficking, Daniel Richard Gulbranson

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Cargo proteins moving between organelles are transported by membrane-enclosed vesicles. Identifying the factors regulating vesicle-mediated transport remains a major challenge in mammalian cells. Here, we performed unbiased genome-wide CRISPR-Cas9 genetic screens to systematically dissect insulin-dependent translocation of glucose transporters (GLUTs), a classic vesicle transport pathway crucial to mammalian physiology. These screens identified known regulators of the pathway as well as a large number of unknown regulatory factors that we validated in secondary screens. The identified genes encode established or predicted factors involved in vesicle budding or fusion, cargo sorting, signal transduction, cell motility, and cellular metabolism, as well as proteins ...


In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski Jan 2018

In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski

Chemical & Biological Engineering Graduate Theses & Dissertations

This thesis investigated the coupling of lysolipids and functionalized tails for in situ formation of synthetic liposomes designed to enable specific characteristics or behaviors in applications ranging from drug delivery to the advancement of artificial cell development. Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) mediated lipid coupling was improved via incorporation of a photoinitiation system. Here, photo-CuAAC enabled spatiotemporal control over liposome assembly, an over 400-fold increase in formation density, and control over the maximal cross-sectional area of the liposomes formed.

Thiol-Michael mediated lipid coupling was enabled using thiol-functionalized lysolipids and acrylate tails where phospholipids were produced over 48 hours with approximate 90 ...


Targets And Functions Of The Microrna-200 Family In The Developing Skin And Hair Follicle, Jaimee Elizabeth Hoefert Jan 2018

Targets And Functions Of The Microrna-200 Family In The Developing Skin And Hair Follicle, Jaimee Elizabeth Hoefert

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The microRNA-200 (miR-200) family is well known for preventing epithelial-to-mesenchymal transition in cancer. However, the targets and functions of this family in normal epithelial tissues remain unclear. This five-member microRNA (miRNA) family also presents a unique platform for studying miRNA-mediated regulation, as they share two nearly-identical seed sequences. The results presented within this dissertation establish a role for these miRNAs in governing hair follicle morphogenesis and fine-tuning cell specification by regulating cell adhesion, polarity, and signaling pathways. By directly ligating miRNAs to their targeted mRNA regions, numerous miR-200 family targets are identified, many of which are involved ...


Novel Factors At Endoplasmic Reticulum-Endosome Contact Sites, Melissa J. Hoyer Jan 2018

Novel Factors At Endoplasmic Reticulum-Endosome Contact Sites, Melissa J. Hoyer

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The endoplasmic reticulum is the cell’s platform for protein and lipid synthesis. Not only does the ER perform these functions, but it also regulates other organelles through membrane contact sites. To better understand the functions of ER membrane contact sites (MCSs), we optimized tools to monitor contact sites and identify new proteins at these MCSs. We recently showed ER MCSs mark positions of the fission of other organelles. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to the endosome budding domains that form from the endosome body and undergo fission from ...


Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi Jan 2018

Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi

Undergraduate Honors Theses

Heart disease continues to be the leading cause of death in both men and women. It is accompanied with cellular- and organ- level remodeling that cannot be mimicked or recaptured by cells on standard culture platforms. Polyethylene glycol hydrogels bridge this gap by providing user-defined tunability for cell culture. A novel 3D cell culture system was developed to encapsulate neonatal rat ventricular cardiomyocytes (NRVMs) in a single plane, and cell health was assessed. In parallel, recombinant adenoviruses containing the mutations responsible for human hypertrophic cardiomyopathy (HCM) in the beta myosin gene (R403Q and R453C) were generated and used as a ...


Investigations Of Interactions Between The Microtubule Binding Domain Of Centrosomal P4.1 Associated Protein And Polyglutamylated Microtubules, Caroline Rhoads Jan 2018

Investigations Of Interactions Between The Microtubule Binding Domain Of Centrosomal P4.1 Associated Protein And Polyglutamylated Microtubules, Caroline Rhoads

Undergraduate Honors Theses

Microtubules maintain particularly diverse and important functions within the cell despite being polymers made up of two highly conserved proteins: a- and b-tubulin. Some of these diverse roles are explained by the presence of post-translational modifications occurring on the C-terminal tail of tubulin proteins, primarily detyrosination, polyglutamylation and polyglycylation. Misregulation of C-terminal tail post-translational modifications are observed in disease phenotypes and may increase the risk of some forms of cancers; however, the role of these post-translational modifications is not well understood. Working towards a goal of designing in vivo methods to study specific post-translational modifications, this paper examines the specificity ...


Fibroblast Growth Factor Receptor Function In Muscle Stem Cells, Tashi Hackett Jan 2018

Fibroblast Growth Factor Receptor Function In Muscle Stem Cells, Tashi Hackett

Undergraduate Honors Theses

Skeletal muscle has a remarkable ability to regenerate itself following injury and this is reliant upon a group of stem cells known as muscle stem cells Muscle stem cell dysfunction contributes to variety of muscle wasting diseases and insights into mechanisms that regulate MuSC function could lead to new therapies and further our understanding of stem cell biology in general. Fibroblast Growth Factor 2 (FGF2) is a protein that regulates muscle stem cell behavior through stimulation of cell surface tyrosine receptor kinases known as Fibroblast Growth Factor Receptors (Jones, 2001). Activation of the receptors by FGF will induce intracellular signaling ...


Novel Insights Into Tace Regulation Of Autocrine And Paracrine Signaling In Epithelial Cells, Eric Newman Bunker Jan 2017

Novel Insights Into Tace Regulation Of Autocrine And Paracrine Signaling In Epithelial Cells, Eric Newman Bunker

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

A multitude of cellular functions are controlled by the activity of the membrane-bound

protease TACE, including immune response and development. Through cleavage of its

membrane anchored substrates, TACE can activate a myriad of signaling pathways in

an auto- or paracrine manner, transferring signals across a tissues and systems.

Despite its importance, it is highly debated how TACE is regulated. I helped generate

TSen, a fluorescence-based sensor of TACE activity, a critical tool that proved

necessary to interrogate the underlying signaling that leads to TACE activation. TSen

enabled us to uncover a wide variation between cell lines and their underlying signaling ...


Disruption Of P53 Activated Transcription By A Rationally Designed Peptide, Benjamin Levi Allen Jan 2017

Disruption Of P53 Activated Transcription By A Rationally Designed Peptide, Benjamin Levi Allen

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

Transcription is a key checkpoint in regulating cell processes. The activity of RNA Pol II is modulated through a variety of mechanisms at various stages of transcription, and the Mediator complex is a central player in carrying out these changes. Mediator is a 1.2 MDa complex composed of 30 subunits. A primary function of Mediator is to bridge the activation domain of a transcription factor bound at an enhancer with Pol II and the general transcription factors at a promoter. Mediator is therefore a direct conduit for transcription factors to activate their target genes.

Chapter 2 describes the design ...


Engineering And Evaluating Fluorescent Tools For Endoplasmic Reticulum Zinc, Kyle Pierce Carter Jan 2017

Engineering And Evaluating Fluorescent Tools For Endoplasmic Reticulum Zinc, Kyle Pierce Carter

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

Zinc (Zn2+) is an essential micronutrient for human health. However, excess Zn2+ is toxic and Zn2+ deficiency can be fatal, so the concentration of this ion must be carefully controlled within cells. Highlighting the importance of Zn2+ is the observation that approximately 10 % of the genes in the human genome potentially code for a Zn2+-binding protein. The Palmer lab has developed a suite of genetically-encoded biosensors for Zn2+ that use Förster Resonance Energy Transfer (FRET) to provide a readout of Zn2+ concentration within human cells. Measurements with these sensors have revealed heterogeneous ...


Neural Crest Cell Development And Evolution, Tyler Alexander Square Jan 2017

Neural Crest Cell Development And Evolution, Tyler Alexander Square

Ecology & Evolutionary Biology Graduate Theses & Dissertations

Neural crest cells play a major role in vertebrate development and evolution. Both the appearance and diversification of these cells have been evoked as a major driver of vertebrate success. This cell type is unique because it is capable of contributing to extremely diverse tissues: structural tissues like cartilage and bone, signal transducing cells of the peripheral nervous system, and multiple types of pigment cells found throughout the body -- in every vertebrate hair, feather, and scale. Elaborations of the biological systems that rely on these cell types were critical in the specialization of vertebrates, as evidenced in fossil and extant ...


Tissue Retention Of Norrin: A Ligand Required For Retinal Vascularization, Lavan Khandan Jan 2017

Tissue Retention Of Norrin: A Ligand Required For Retinal Vascularization, Lavan Khandan

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The Norrin ligand is a small (14 kDa) cystine-knot protein, required for the proper vascularization of retinal, cerebellar and inner ear neural tissue. Both inherited human disorders and murine genetic studies implicate Norrin mutations in major ocular and visual defects, including retinal hypovascularization. In this thesis, I describe the property of Norrin enrichment at specific layers in the retina. I address the question of whether proper Norrin distribution in retinal tissue is required for vascularization. To do so, I used ex vivo ligand in-situ staining to interrogate differences amongst vertebrate Norrin orthologues in retinal tissue retention. I then used sequence ...


Elucidating The Ligand-Specific Role Of Tetraspanin12 As An Essential Co-Activator In Norrin/Frizzled4 Signaling And Retinal Vascularization, Maria B. Lai Jan 2017

Elucidating The Ligand-Specific Role Of Tetraspanin12 As An Essential Co-Activator In Norrin/Frizzled4 Signaling And Retinal Vascularization, Maria B. Lai

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Genetic evidence indicates that specific combinations of accessory proteins and ligands mediate vascular Frizzled (FZD) signaling via beta-catenin in different CNS structures. Accessory proteins in FZD receptor complexes are thought to determine ligand-selectivity and signaling amplitude. In the retina, TSPAN12 is an essential co-activator in Norrin/FZD4 signaling to mediate angiogenesis. The genes encoding mediators of Norrin/FZD4 signaling are linked to familial exudative vitreoretinopathy (FEVR), an inherited retinal disease that can lead to blindness. Yet, the molecular function of TSPAN12 and the specific cell type in which TSPAN12 functions in the retina remains poorly understood. Here, I utilized binding ...


Identification And Characterization Of Regulators Of Glut4 Trafficking, Daniel Richard Gulbranson Jan 2017

Identification And Characterization Of Regulators Of Glut4 Trafficking, Daniel Richard Gulbranson

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Cargo proteins moving between organelles are transported by membrane-enclosed vesicles. Identifying the factors regulating vesicle-mediated transport remains a major challenge in mammalian cells. Here, we performed unbiased genome-wide CRISPR-Cas9 genetic screens to systematically dissect insulin-dependent translocation of glucose transporters (GLUTs), a classic vesicle transport pathway crucial to mammalian physiology. These screens identified known regulators of the pathway as well as a large number of unknown regulatory factors that we validated in secondary screens. The identified genes encode established or predicted factors involved in vesicle budding or fusion, cargo sorting, signal transduction, cell motility, and cellular metabolism, as well as proteins ...


Correlative Light And Electron Microscopy On Mouse Polyomavirus-Infected Fibroblasts, Christina Henderson Jan 2017

Correlative Light And Electron Microscopy On Mouse Polyomavirus-Infected Fibroblasts, Christina Henderson

Undergraduate Honors Theses

Polyomaviruses are small, DNA tumor viruses that can infect multiple species, including humans. In recent years, the number of identified human polyomaviruses (huPyV) has grown. To identify possible therapeutic targets for huPyVs, a better understanding of virus infection at the structural level is needed. Confocal microscopy allows for the visualization of fluorescently-tagged proteins but without seeing the cell’s subcellular structures, functional information can be lost. Electron microscopy provides structural insight but does not always provide biological or biochemical details. Combining these two approaches can relate biological function to structural information about virus replication. Polyomaviruses (PyV) co-opt host proteins that ...


Homothorax Is A Modifier Of Radiation Sensitivity In Drosophila Melanogaster Bantam Mutants, Geoffrey Meyerhof Jan 2017

Homothorax Is A Modifier Of Radiation Sensitivity In Drosophila Melanogaster Bantam Mutants, Geoffrey Meyerhof

Undergraduate Honors Theses

Radiation resistance in human cancers represents a massive impediment for successful tumor treatment. The fruit fly Drosophila melanogaster is an excellent model for human radiation resistance because of its largely conserved apoptotic pathways and malleable genome. This thesis investigates the genetic regulatory mechanisms for bantam (ban), an anti-apoptotic microRNA. To first identify genes that interact with ban, a forward genetic screen was conducted. This screen looked for genes that yielded radiation dependent pupal lethality in a ban deficient background. From this screen the transcription factor, homothorax, was identified as displaying radiation dependent synthetic lethality with ban. To investigate the mechanism ...


Characterization Of Tumor Protein P63 Regulated 1-Like Function In Xenopus Laevis, Julia Mo Jan 2017

Characterization Of Tumor Protein P63 Regulated 1-Like Function In Xenopus Laevis, Julia Mo

Undergraduate Honors Theses

Of the approximately 20,000 genes in the human genome, about 6,000 have unknown or poorly characterized function. Tumor protein p63 regulated 1-like (TPRG1l) is one of those genes with no functional data. TPRG1L is expressed during embryonic development and adulthood with strongest expression in the brain. It is implicated in carcinogenesis because its gene expression is activated by tumor regulators p63/73. To elucidate TPRG1L function during embryogenesis, CRISPR-Cas9 genome editing system was utilized to induce Tprg1l mutations in Xenopus laevis. Embryos injected with Tprg1l sgRNA and Cas9 mRNA displayed axis formation and convergent extension defects suggesting that ...


An Inquiry Into The Electro-Physiology Of Bacteria, Prasanth Prahladan Apr 2016

An Inquiry Into The Electro-Physiology Of Bacteria, Prasanth Prahladan

Electrical, Computer & Energy Engineering Graduate Theses & Dissertations

This thesis documents the exploration of tools and techniques which were used to determine the existence of patterns within and relationships between the voltage and calcium measurements from individual bacterial cells. The research lies in the broad domain of bacterial electro-physiology.


Analysis Of Two Centrin-Binding Proteins, Poc5 And Sfr1, In Tetrahymena Thermophila Basal Bodies, Westley Heydeck Jan 2016

Analysis Of Two Centrin-Binding Proteins, Poc5 And Sfr1, In Tetrahymena Thermophila Basal Bodies, Westley Heydeck

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Basal bodies are microtubule-based structures which template, anchor, and orient cilia at the cell surface. Although basal bodies contribute to vital cell functions, the molecular contributors of their assembly and maintenance are poorly understood. Previous studies in Tetrahymena thermophila revealed important roles for centrins in basal body assembly, separation of new basal bodies, and stability. Here, I characterized the basal body function of two centrin-binding proteins, Sfr1 and Poc5, in Tetrahymena. Sfr1 is the only centrin-binding protein in Tetrahymena that localizes to all cortical row and oral apparatus basal bodies. Poc5, on the other hand, transiently localizes to basal bodies ...


Polyomavirus Interactions With Host Cell Surface Receptors Mediate Important Steps In Virus Infection: From Signaling To Pathogenesis, Samantha D. O'Hara Jan 2016

Polyomavirus Interactions With Host Cell Surface Receptors Mediate Important Steps In Virus Infection: From Signaling To Pathogenesis, Samantha D. O'Hara

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Virus binding to the cell surface triggers an array of host responses important for infection. Gangliosides are the cell surface receptors for Polyomavirus (PyV) infection. Specificity is determined by recognition of carbohydrate moieties on the ganglioside by the major viral capsid protein VP1 and alterations in ganglioside binding cause dramatic changes in virus tropism and pathogenesis. Knockout mice lacking complex gangliosides are completely resistant to Mouse Polyomavirus (MuPyV) infection. Fibroblasts (MEFs) from these mice are likewise resistant to infection, and supplementation with specific gangliosides: GD1a, GT1b, and GT1a rescues infection. MuPyV also binds a protein receptor α4-integrin and loss of ...


Double Knockout Of Munc18b And Munc18c Reduces Translocation Of Glut4 In Response To Insulin, Zoe A. Praggastis Jan 2016

Double Knockout Of Munc18b And Munc18c Reduces Translocation Of Glut4 In Response To Insulin, Zoe A. Praggastis

Undergraduate Honors Theses

Insulin resistance has become a prominent issue in the United States, causing serious comorbid conditions. One crucial step in insulin response in adipose and muscle tissue is the movement of glucose transporter GLUT4 to the cell surface from storage vesicles. This process requires the SNARE complex to drive membrane fusion. Munc18b and Munc18c are syntaxin binding proteins associated with the SNARE complex during fusion. While protein binding assays have revealed interactions of these proteins, their function in GLUT4 translocation has not been well understood. In an effort to establish the role of these proteins in GLUT4 translocation and insulin response ...


With Or Without You: Studying The Requirement Of P53 For Anti-Cancer Responses To Nuclear Export Inhibitors, Andrea E. Doak Jan 2016

With Or Without You: Studying The Requirement Of P53 For Anti-Cancer Responses To Nuclear Export Inhibitors, Andrea E. Doak

Undergraduate Honors Theses

Exportin-1 (XPO-1) is responsible for the movement of cargo proteins out of the nucleus and into the cytoplasm. Selective inhibitors of nuclear export (SINE) bind XPO-1 at cysteine-528, which results in the sequestration of cargo proteins in the nucleus. SINE drugs are currently being developed and tested in the treatment of many types of cancers. One of the cargos, p53 may play an important role in the efficacy of SINE. To test the necessity of p53 in the action of SINE drugs, matched pairs of cell lines with wildtype or functionally disrupted p53 were analyzed for differences in their cell ...


Endoplasmic Reticulum Area Expands Upon Onset Of The Unfolded Protein Response, Katelyn Camille Cook Jan 2016

Endoplasmic Reticulum Area Expands Upon Onset Of The Unfolded Protein Response, Katelyn Camille Cook

Undergraduate Honors Theses

The endoplasmic reticulum (ER) is a dynamic organelle responsible for essential cell functions such as protein folding, calcium storage, and lipid synthesis. It also directly interacts with and regulates other organelles in the cell, such as endosomes or mitochondria, and is the site of bulk protein synthesis for both secretion and the cellular endomembrane system. However, cellular stressors such as heat shock, chemical imbalance, or calcium depletion can disrupt the protein-folding capacity of the ER, causing activation of the ER-mediated unfolded protein response (UPR) in order to either restore homeostasis or initiate apoptosis. Chronic ER stress is a hallmark of ...


Investigating The Molecular Mechanisms And Functions Of The Musashi-2 Rna-Binding Protein, Christopher Bennett Jan 2016

Investigating The Molecular Mechanisms And Functions Of The Musashi-2 Rna-Binding Protein, Christopher Bennett

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The Musashi (Msi) family of RNA-binding proteins is post-transcriptional regulators of gene expression. They were discovered in 1994 as being required for Drosophila sensory organ development. Since then, Msi proteins have been found to enhance cell proliferation and maintain stem cell identities in a multitude of mammalian tissues. In addition, overexpression of Msi proteins is often observed in many types of human cancers, most prominently the widely expressed Msi family member, Musashi-2 (Msi2). Msi2 plays oncogenic roles in hematopoietic, neural, and gastrointestinal tissues. However, Msi2 has received little attention in other tissues in which it is expressed, such as in ...


Activation And Utilization Of Dna Damage Signaling By Murine Polyomavirus, Katie Heiser Jan 2016

Activation And Utilization Of Dna Damage Signaling By Murine Polyomavirus, Katie Heiser

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Nuclear replication of DNA viruses activates DNA damage repair (DDR) pathways, which may detect and inhibit viral replication. However, many DNA viruses also depend on these pathways in order to optimally replicate their genomes. I investigated the relationship between murine polyomavirus (MuPyV) and components of DDR signaling pathways including CHK1, CHK2, H2AX, ATR, ATM, RPA, MRN, and DNAPK. I found that recruitment and retention of DDR proteins at viral replication centers was independent of H2AX, as well as the viral small and middle T-antigens. Additionally, infectious virus production required ATR kinase activity, but was independent of CHK1, CHK2, or DNAPK ...


Stability Of Escrt Iii Snf7 Polymer Influenced By: Bro1 And Vps2/Vps24, Troy K. Coody Iii Jan 2016

Stability Of Escrt Iii Snf7 Polymer Influenced By: Bro1 And Vps2/Vps24, Troy K. Coody Iii

Undergraduate Honors Theses

The endosomal sorting complexes required for transport (ESCRT) are protein complexes that remodel cellular membranes. The ESCRT III membrane machinery has been implicated in regulating membrane bound receptor proteins through the multi-vesicular body (MVB) pathway, during the end of cytokinesis, and being manipulated by the HIV virus during infection. My thesis project focuses on the in vivo regulation of ESCRT III. Bro1 binding to the Snf7 protein polymer helped stabilize ESCRT III. With this understanding that Bro 1 stabilizes the Snf7 polymer, two questions can be posed: Does it block Vps4 mediated Snf7 disassembly? Or does it block Vps2 and ...


Synaptogyrin 3 Binds Amyloid Beta And May Alter Dopamine Transport In Alzheimer’S Disease, William Gierach Jan 2016

Synaptogyrin 3 Binds Amyloid Beta And May Alter Dopamine Transport In Alzheimer’S Disease, William Gierach

Undergraduate Honors Theses

Alzheimer’s Disease effects 35 million elderly individuals globally by causing dementia, memory loss and eventually death. Amyloid Beta (Aß), a neurotropic peptide and a hallmark of AD pathogenesis, disrupts cellular organization and impairs neuronal systems. The dopaminergic system regulates learning, motor function and behavior and malfunctions in early AD. Aß causes dopaminergic transmission to dysfunction by unknown mechanisms. To elucidate these mechanisms, we screened solubilized mouse synaptosomes for proteins that specifically bind Aß 1-42. 107 proteins that bound Aß were identified by mass spectrometry. Human orthologs were further screened in HEK293 cells for Aß binding. Of the thirteen proteins ...