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Full-Text Articles in Cell Biology

Id3, Estrogenic Chemicals, And The Pathogenesis Of Tumor-Like Proliferative Vascular Lesions, Vincent E. Avecilla Oct 2017

Id3, Estrogenic Chemicals, And The Pathogenesis Of Tumor-Like Proliferative Vascular Lesions, Vincent E. Avecilla

FIU Electronic Theses and Dissertations

Tumor-like proliferative vascular lesions manifest in several diseases such as peripheral arterial disease (PAD) and atherosclerosis (AS) after arterial injury. The cause of the vascular cell dysfunction in PAD patients is not known. Our recent novel discovery shows that inhibitor of differentiation 3 (ID3) is highly expressed in intimal lesions of clinical vascular disease samples. The central hypothesis of our study is: estrogenic chemical induced dysregulation of ID3 target genes is involved in the development of vascular disease. NHANES data analysis demonstrated higher geometric levels of all 6 PCB congeners in both PAD diagnosed participants and participants at risk of ...


Transgneic Endothelin 3 Regulates Murine Pigment Production And Coat Color, Javier Pino Oct 2017

Transgneic Endothelin 3 Regulates Murine Pigment Production And Coat Color, Javier Pino

FIU Electronic Theses and Dissertations

Pigmentation plays a protective role against damage caused by ultraviolet (UV) irradiation. Humans with fair skin and light hair have a higher susceptibility to UV-induced DNA damage that can lead to the development of skin cancers. The melanocytes found in the skin and hair follicles depend on different signaling molecules for their proper development and pigment production. α-Melanocyte Stimulating Hormone (α-msh) binds to the Melanocortin 1 receptor (Mc1r) to regulate pigment production and the switch between eumelanin and pheomelanin. Lethal yellow mice (Ay) overexpress the agouti signaling protein, which inhibits the binding of α-msh, resulting in a yellow coat ...


Regulation Of Adipocyte Differentiation And Metabolism: Rab5-Guanine Nucleotide Exchange Factors And Methylglyoxal, Praew Chantarasinlapin Mar 2017

Regulation Of Adipocyte Differentiation And Metabolism: Rab5-Guanine Nucleotide Exchange Factors And Methylglyoxal, Praew Chantarasinlapin

FIU Electronic Theses and Dissertations

Internalization and trafficking of ligand-receptor complex rely on a particular set of proteins, e.g. small GTPase protein Rab5 and its activators called guanine nucleotide exchange factors. Rab5-activating protein 6 (RAP6), a Vps9-containing protein, may participate in Rab5-mediated insulin signaling and receptor trafficking. A dicarbonyl compound methylglyoxal was found to alter insulin signaling in preadipocytes. This dissertation aimed to investigate the association of RAP6 activity on 3T3-L1 preadipocyte differentiation and those driven by methylglyoxal. Overexpression of RAP6 inhibited preadipocyte differentiation, Ser473-phosphorylation of Akt1, and expression of adipogenic marker PPARγ, but not C/EBPα. Methylglyoxal (10 µM) increased preadipocyte differentiation, proliferation ...


The Effects Of Artemisia Derived Natural Products On Adipogenesis, Steven Abood Jan 2017

The Effects Of Artemisia Derived Natural Products On Adipogenesis, Steven Abood

FIU Electronic Theses and Dissertations

For the first time in human history, more people worldwide suffer from obesity than are undernourished. Numerous health complications are associated with obesity including cardiovascular disease, Type 2 Diabetes, cancers of reproductive tissues, stroke, depression, anxiety disorders, and Alzheimer’s disease. A deeper understanding of the anti-adipogenic effects and mechanism of action of sesquiterpene lactones may have pharmacological import in the continuing search for therapeutic modalities to ameliorate the effects of this global obesity epidemic.

Dehydroleucodine (DhL), 11,13-dihydro-dehydroleucodine (DH-DhL), and dehydroparashin-B (DhP), sesquiterpene lactones extracted from or derived from compounds extracted from Artemisia douglasiana, were investigated for their anti-adipogenic ...