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Full-Text Articles in Cell Biology

Effects Of Fatty Acids From Coconut And Olive Oil On Expression Of The Hmg1 Gene And Feeding Behavior In Tetrahymena Thermophila, Amelia Konen, Taryn Stewart Apr 2019

Effects Of Fatty Acids From Coconut And Olive Oil On Expression Of The Hmg1 Gene And Feeding Behavior In Tetrahymena Thermophila, Amelia Konen, Taryn Stewart

Carroll College Student Undergraduate Research Festival

HMG-CoA reductase is an enzyme involved in the biosynthetic pathways of sterols in many different organisms. The gene in Tetrahymena thermophila which codes for this protein is HMG1, and has many homologs across many other organisms, from fungi to humans. For this project, we wanted to look at the effect of external fatty acids from coconut and olive oil on the expression of HMG1 in T. thermophila. Previous research has shown that exposure to unsaturated oils result in decreased cholesterol levels. We hypothesized that if T. thermophila are introduced to saturated fats and unsaturated fats, they would exhibit a respectively ...


Effects Of The Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, Maria Carparelli, Kelsey Ripley, Stefanie Otto-Hitt Jul 2018

Effects Of The Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, Maria Carparelli, Kelsey Ripley, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Titanium dioxide (TiO2) is a widely used compound found in everything from food packaging to sunscreens. When ingested, TiO2 is readily transported across membranes and efficiently stored within cells. Previous studies showed that exposure to TiO2 results in underdeveloped nervous systems. For our study, we attempted to answer the following question: Will exposing Drosophila melanogaster larvae to TiO2 affect expression of the Neur gene and development of the central nervous system? The Neur gene is crucial during the celldetermination stage of development as its encoded protein helps specify neuroblast development and aids in nervous system and sensory organ development. It ...


Effects Of Salt Concentration On Tetrahymena Thermophila Growth And Crp1 Gene Expression, Katie Mchugh, Rachel Tremaine, Anna Sapone, Stefanie Otto-Hitt Jul 2018

Effects Of Salt Concentration On Tetrahymena Thermophila Growth And Crp1 Gene Expression, Katie Mchugh, Rachel Tremaine, Anna Sapone, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The use of salt to remove ice from roads has resulted in increased salinity in many freshwater lakes in North America. For this project, we wanted to explore how environmental salt concentrations influence the rate of growth of Tetrahymena thermophila and expression of the CRP1 gene. We hypothesized that if the sodium concentration is increased in the media, Tetrahymena growth would decrease and expression of the CRP1 gene would increase. The CRP1 gene encodes a protein that helps regulate calcium concentrations within a cell based on the concentration of sodium ions. The media of the experimental group was treated with ...


Uv Light Exposure And Tetrahymena Thermophilia: Expression Of The Phr2 Gene, Molly Moloney, Tanner Gustavsen, Stefanie Otto-Hitt Jul 2018

Uv Light Exposure And Tetrahymena Thermophilia: Expression Of The Phr2 Gene, Molly Moloney, Tanner Gustavsen, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The PHR2 (photolyase repair) gene functions in the repair of DNA in Tetrahymena thermophila. It was hypothesized that when Tetrahymena cultures are exposed to UV light, increased expression of PHR2 would occur in response to the damage incurred. UV light has been known to cause the formation of pyrimidine dimers and other disruptions in DNA. Through two rounds of experimentation, the acute impact of UV light exposure was monitored. The cultures underwent RNA extraction, reverse transcription, and semi-quantitative PCR analysis. We predicted that UV exposure would result in the increased expression of PHR2.


Effect Of Potassium Bromate On Oxr1 Gene Expression And Cell Growth In Tetrahymena Thermophila, Scott Khale, Brendan Mcmahon, Stefanie Otto-Hitt Jul 2018

Effect Of Potassium Bromate On Oxr1 Gene Expression And Cell Growth In Tetrahymena Thermophila, Scott Khale, Brendan Mcmahon, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The purpose of our experiment was to answer the question: Does oxidative damage, induced by potassium bromate (KBrO3), affect growth rate and expression of the OXR1 gene in Tetrahymena thermophila? Potassium Bromate was chosen as the environmental stressor because it is a strong oxidizing agent that is used as a leavening agent in baked goods. It was hypothesized that expression of OXR1 would increase in Tetrahymena cells that were exposed to potassium bromate and that their growth rate would decrease. The exact mechanism and function of the OXR1 gene is still unknown; however, the literature suggests that it is required ...


Analysis Of Fbp1 Gene Expression In Uv Light-Exposed Tetrahymena Thermophila Cultures, Colby Henry, Stefanie Otto-Hitt Jul 2018

Analysis Of Fbp1 Gene Expression In Uv Light-Exposed Tetrahymena Thermophila Cultures, Colby Henry, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this study, Tetrahymena thermophila cultures were exposed to an acute treatment of UV light and expression of the FBP1 gene was examined. The FBP1 gene encodes Fructose- 1,6-biphosphatase which plays an important role in the process of gluconeogenesis. It was hypothesized that exposure of Tetrahymena to UV light would cause an upregulation in DNA repair enzymes, thereby causing these cells to require higher levels of glucose. The higher demand for glucose would then result in the upregulation of gluconeogenesis and FBP1. Following acute exposure of Tetrahymena cultures to UV light, the expression of FBP1 was analyzed using reverse ...


Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt Jul 2018

Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Scramblase is an enzyme that facilitates the movement of newly synthesized phospholipids from the cytosolic side to the extracellular side of the lipid bilayer. This process is vital for cell membrane repair and growth. In Tetrahymena thermophila, the gene SIT1 encodes for the Scramblase protein, whose functionality is Ca2+-dependent. In this experiment, the concentration of accessible Ca2+ ions was decreased in order to observe whether the change had an aect on the expression of SIT1 and cell growth. It was hypothesized that expression of the SIT1 gene would increase, while cell growth would decrease. To carry out the experiment ...


Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis, Stefanie Otto-Hitt Jul 2018

Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. For this experiment, it was hypothesized that alcohol induces starvation due to a lack of energy available in the organism for feeding. This hypothesis was tested by monitoring food vacuole formation and expression of the PFK1 gene in Tetrahymena that were exposed to alcohol. The PFK-1 gene was chosen because its encoded protein plays an essential role in cellular metabolism. For the experiment, control and experimental cultures of Tetrahymena themophila were maintained in a nutrient rich media, with the media of the experimental group ...


The Effect Of Metal Pollutants On Cell Growth And Ttherm0058490expressionint Etrahymenathermophila, Inderbir Bains, William French, Stefanie Otto-Hitt Jul 2018

The Effect Of Metal Pollutants On Cell Growth And Ttherm0058490expressionint Etrahymenathermophila, Inderbir Bains, William French, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, we sought to answer the following question: Do higher levels of metal exposure affect expression of the TTHERM 00584900 gene in Tetrahymena thermophila? The TTHERM 00584900 gene produces an ATP-binding cassette protein that is responsible for removing metals and metalloids from Tetrahymena thermophila. It is hypothesized that the expression of TTHERM 00584900 will increase with exposure to metals. In order to test this hypothesis, Tetrahymena thermophila were randomly assigned to either a control group, which was cultured in a normal growth medium, or a treatment group, whichin addition to the medium was exposed to water contaminated with ...


Impacts Of Environmental Motion On Cdc2 Expression And Cell Growth In Tetrahymena Thermophila, Jack Rees, Keaton Brady, Stefanie Otto-Hitt Jul 2018

Impacts Of Environmental Motion On Cdc2 Expression And Cell Growth In Tetrahymena Thermophila, Jack Rees, Keaton Brady, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, the eect of constant motion on expression of the cell cycle control gene CDC2 was examined in the organism Tetrahymena thermophila. We hypothesized that CDC2 expression would decrease with a corresponding increase in movement within the Tetrahymena culture environment. The CDC2 protein is primarily used in the replication of eukaryotic DNA because of its connection to HHO1, an important factor in transcriptional regulation. If the cells were subject to vegetative growth in which there are no stressors put on the cell, then HHO1 becomes phosphorylated to prevent it from interacting with chromatin. Because CDC2 is important in ...


Effects Of Fatty Acids From Avocados On Expression Of The Hmg1 Gene In Tetrahymena Thermophila, Jasmine Phan, Connor Kane, Stefanie Otto-Hitt Jul 2018

Effects Of Fatty Acids From Avocados On Expression Of The Hmg1 Gene In Tetrahymena Thermophila, Jasmine Phan, Connor Kane, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The enzyme 3-Hydroxy-3-Methyl-Glutaryl-CoA Reductase (HMG) HMG catalyzes the ratelimiting step in the biosynthesis of cholesterol and is a common drug target for cholesterol reduction. Previous studies in rats have shown that avocado oil reduces cholesterol levels. We were interested in determining whether the healthy fatty acids in avocado oil would have an effect on the expression of HMG. In this study, Tetrahymena thermophila were treated with avocado oil to determine the effect of fatty acids on expression of the HMG1 gene that encodes HMG. Expression of HMG1 was monitored using reverse transcription and semi-quantitative PCR. We predicted that expression of ...


Exploring The Effects Of Environmental Temperature On Cdc-7 Expression In T. Thermophila, John Brothers, Alexis Gerry, Lauren Scofield, Stefanie Otto-Hitt Jul 2018

Exploring The Effects Of Environmental Temperature On Cdc-7 Expression In T. Thermophila, John Brothers, Alexis Gerry, Lauren Scofield, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Tetrahymena thermophila are unicellular eukaryotes with a remarkably large genome: over 27,000 genes. One important aspect of studying the Tetrahymena genome is identifying and understanding what factors influence gene expression. This study examined the effects of temperature on the expression of CDC-7, a cell division control gene. We subjected Tetrahymena cultures to increased temperature and hypothesized such treatments would induce cell division and increase CDC-7 expression. Gene expression levels were measured via semi-quantitative PCR analysis after an acute heat treatment.


The Effect Of Basic Conditions On Cdk1 Expression In Tetrahymena Thermophila, Kaitlin Stromberg, Jakob Plagenz, Stefanie Otto-Hitt Jul 2018

The Effect Of Basic Conditions On Cdk1 Expression In Tetrahymena Thermophila, Kaitlin Stromberg, Jakob Plagenz, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The goal of this project was to discover whether expression of the CDK1 gene in Tetrahymena thermophila, as well as their growth, are aected by changing the pH of the growth media. We hypothesized that expression of the CDK1 gene would decrease significantly when grown in basic media. The CDK1 gene encodes the protein Cyclin-Dependent Kinase, which functions to advance cells through various stages of the cell cycle. To test our hypothesis, Tetrahymena cultures were randomly assigned to either the control group, which was grown in media with a neutral pH, or a treatment group, which was grown in media ...


The Effect Of Exposure To Uv Light On Rad51 Expression In Tetrahymena Thermophila, Kendall Patch, Kerri Mcinnis, Stefanie Otto-Hitt Jul 2018

The Effect Of Exposure To Uv Light On Rad51 Expression In Tetrahymena Thermophila, Kendall Patch, Kerri Mcinnis, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, we attempted to answer the question: Does exposure to UV light affect expressionof the Rad51 gene in Tetrahymena thermophila? It was hypothesized that the expression of Rad51 would increase proportionately to the exposure of UV light. The Rad51 protein is fundamental in the repair of double-stranded DNA breaks in eukaryotes, specifically during the process of homologous recombination. In order to test this hypothesis, Tetrahymena were randomly assigned to either a control group, which was cultured under ideal conditions, or a treatment group, which was exposed to UV light. Previous experiments have suggested that T. thermophila prosper most ...


Analyzing Atp Synthetase F1 Expression In Heat-Exposed Tetrahymena Thermophila, Kevin Gardner, Ian Lorang, Stefanie Otto-Hitt Jul 2018

Analyzing Atp Synthetase F1 Expression In Heat-Exposed Tetrahymena Thermophila, Kevin Gardner, Ian Lorang, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this study, we investigated the effect of temperature on expression of the ATP1 gene in the organism Tetrahymena thermophila. We hypothesized that expression of the ATP1 gene would increase as we increased temperature. ATP1 is a highly conserved gene in Tetrahymena that codes for the alpha subunit of ATP Synthase, an enzyme that generates the energy molecule ATP. We decided to use heat treatment based on studies performed on ATP1 in Alaskan Cod showing increased expression in response to increased temperatures (Michael et al., 2015). We used reverse transcription and semi-quantitative PCR to analyze expression of ATP1 in our ...


Effect Of Nitrate Pollution On Tetrahymena Thermophila, Reegan Debruycker, Hannah Sylvester, Stefanie Otto-Hitt Jul 2018

Effect Of Nitrate Pollution On Tetrahymena Thermophila, Reegan Debruycker, Hannah Sylvester, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The question under investigation in this project was: Does an elevated concentration of nitrates have a negative eect on cell growth and expression of the CDC16 gene in the organism Tetrahymena thermophila? Exposure of Tetrahymena cultures to nitrates mimics the exposure of these organisms to nitrate pollution in the environment, which can occur due to septic tank leakage, nitrogen-rich fertilizer run o, and agricultural processes. The EPA standard for nitrate concentrations in drinking water is a maximum of 10ppm. In our experiment, Tetrahymena cultures were treated with media that contained 30ppm of nitrates for one week. The 30ppm nitrate concentration ...


The Effect Of Exposure To Ionizing Radiation On Cwf16 Expression In Tetrahymena Thermophila, Shelby Olsen, David Woolston Jul 2018

The Effect Of Exposure To Ionizing Radiation On Cwf16 Expression In Tetrahymena Thermophila, Shelby Olsen, David Woolston

Life and Environmental Sciences Course-based Research Projects

The following research seeks to determine whether exposure to ultraviolet radiation will induce an increase in the expression of the Cwf16 gene in Tetrahymena thermophila. Cwf16 is a homolog of Ccgc94, whose gene product is a functioning member of the Prp19 complex, a protein complex which protects cells from committing apoptosisprogrammed cell deathdue to exposure to ionizing radiation. The homology between Cwf16 and Ccgc94 yields the hypothesis that Cwf16 expression in T. thermophila will increase in response to UV radiation exposure in a similar mechanism to that observed the Ccgc94 system. In the present research, T. thermophila cultures were randomly ...


The Effects Of Warming Temperatures On Rad51 Expression In Tetrahymena Thermophila, Timothy Brunson, Rachael Lefstad, Stefanie Otto-Hitt Jul 2018

The Effects Of Warming Temperatures On Rad51 Expression In Tetrahymena Thermophila, Timothy Brunson, Rachael Lefstad, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Our experiment explored whether raised temperatures affected expression of the Rad51 gene in Tetrahymena thermophila. Our hypothesis was that raised temperatures would result in a decrease in expression of the Rad51 gene. The protein product of this gene functions in double stranded DNA repair. We chose this experiment to see if the rising average temperatures around the world would affect expression of genes involved in DNA repair. Our test samples were kept in a growth medium at 37C while our control samples were kept in a growth medium at 24C. Afterwards, the transcribed mRNA was reverse transcribed into DNA and ...


The Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis Apr 2018

The Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis

Carroll College Student Undergraduate Research Festival

The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. Prior research has shown that exposure to alcohol results in decreases in both the frequency of feeding and overall growth. For this experiment, it was hypothesized that these effects are due to a lack of energy available in the organism for feeding. This hypothesis was tested by monitoring food vacuole formation and expression of the PFK-1 gene in Tetrahymena that were exposed to alcohol. The PFK-1 gene was chosen because its encoded protein plays an essential role in cellular metabolism. For the experiment, control ...


Engineering An Improved Expression Vector For The Nupl53 Rna Binding Domain, Francisca Maertens Apr 2007

Engineering An Improved Expression Vector For The Nupl53 Rna Binding Domain, Francisca Maertens

Life and Environmental Sciences Undergraduate Theses

The nucleoporin Nupl53 has a critical role in both import and export of cellular substances across the nuclear envelope. A unique RNA binding domain has been revealed in Nupl53 (Nupl53-RBD). In order to further understand the interaction between the RNA binding domain (RBD) of Nupl53 and RNA, a crystal structure of the domain would be invaluable. This can be accomplished only if a significant quantity of purified RBD is isolated. Several RBD constructs have been produced to aid in purification of this protein. In most cases, recombinant RBD preparations are fairly heterogeneous and thus unsuitable for structural studies. However, a ...


Developing Proteomics Techniques For Glycosylated Proteins, Adrienne Udelhoven Apr 2006

Developing Proteomics Techniques For Glycosylated Proteins, Adrienne Udelhoven

Life and Environmental Sciences Undergraduate Theses

The ability to study proteins and their role in cell function is a central area of scientific study. To learn more about proteins and glycosylation, a form of posttranslational modification, a means of detecting specifically for glycosylation is necessary. The modification of an existing dye to meet this need was the goal ofthe project. N-benzyl-4-nitroaniline and methyl ((4-(4-nitrophenyl)amino)methyl)benzoate, two intermediate compounds in the synthesis, were created and purified.


The Role Of E2f1 In Endocycle Regulation, Lindsay Black Apr 2004

The Role Of E2f1 In Endocycle Regulation, Lindsay Black

Life and Environmental Sciences Undergraduate Theses

In D. melanogaster, CycE and E2F1 are critical proteins in endocycle control. It is known that both of these proteins are required for endocycling and that cycE mRNA and protein oscillate in endoreduplicating tissues (ERT’s). The mechanism of endocycle regulation by E2F1 through CycE, however, has not been elucidated. Here, I implicate E2F1 protein oscillation in the fluctuation of two of its specific target genes, cycE and rnr2, which is then responsible for the G-S transition ofDrosophila ERT’s. The results also suggest that E2F1 control of S phase initiation within the endocycling cell is dependent on nutritional inputs.


Differential Expression Of Mitochondrial Proteins In Alzheimer’S Disease Patients Compared To Age-Matched Controls, Erin Flaherty Apr 2004

Differential Expression Of Mitochondrial Proteins In Alzheimer’S Disease Patients Compared To Age-Matched Controls, Erin Flaherty

Life and Environmental Sciences Undergraduate Theses

To date, the pathology of Alzheimer’s remains unknown, and many researchers have focused on the mitochondria as playing a significant role in its progression. In earlier studies, it was found that mRNA levels of mitochondrial proteins of Alzheimer’s Disease patients were altered in mouse and human models. Additional research is warranted to determine if the mRNA differences are translated to the protein level. It has been shown that mitochondrial genes are differentially expressed in Alzheimer’s patients when compared to age-matched controls. I propose the levels of the translated gene product will also be altered. To test this ...


The Effect Of Pkc 5 Inhibitors On Pma-Induced Apoptosis In Xenopus Laevis Splenocytes, Annie Crater Apr 2003

The Effect Of Pkc 5 Inhibitors On Pma-Induced Apoptosis In Xenopus Laevis Splenocytes, Annie Crater

Life and Environmental Sciences Undergraduate Theses

Protein kinase C (PKC) controls apoptosis in amphibian systems such as that of the South African clawed frog, Xenopus laevis. The effects of cancer inducing agents like phorbol 12-myristate 13-acetate (PMA) when combined with Ca2+ independent PKC inhibitors on programmed cell death were studied in A laevis splenocytes. Regulation of PMA-activated cells was observed via Annexin V-FITC and propidium iodide assays, flow cytometry, and Western blot analysis. Results indicate the PKC inhibitors reduced both apoptosis and cell growth in PMA-stimulated X. laevis splenocytes compared to PMA alone. These results are valuable to future mammalian cancer research. By identifying the various ...


Analysis Of Candidate Proteins Interacting With Eyal, Ryan Schwanke Apr 2002

Analysis Of Candidate Proteins Interacting With Eyal, Ryan Schwanke

Life and Environmental Sciences Undergraduate Theses

The Eyal gene is important for mammalian organogenesis, and mutations in the human EYA1 results in Branchio-Oto-Renal (BOR) syndrome. This syndrome is autosomal dominant and causes combinations of branchial, otic, and renal anomalies. All Eya gene products contain an extremely conserved 271 amino acid C-terminus that is essential in protein-protein interaction. Recently, it was found that during mouse development, the expression of certain Six genes relied on the function of Eyal, and that Eyal interacts with relevant Six proteins through its C-terminal domain. These results provided insight into the molecular and developmental basis for organ defects occurring in BOR syndrome ...


Acid Phosphatase Content And Acidity Of The Perialgal Vacuole Of Ephydatia Muelleri, Camette Miller Apr 1997

Acid Phosphatase Content And Acidity Of The Perialgal Vacuole Of Ephydatia Muelleri, Camette Miller

Life and Environmental Sciences Undergraduate Theses

Freshwater sponges containing symbiotic algae can serve as a model for the study of relationships in which a host and an intracellular symbiont benefit. In freshwater sponges, symbiotic algae belonging to the genus, Chlorella, are housed in large cytoplasmic vacuoles, called perialgal vacuoles. I used microscopic techniques in an effort to determine whether the perialgal vacuoles ofthe freshwater sponge, Ephydatia muelleri, (1) contain active acid phosphatase, a lysosomal enzyme and (2) are acidic. Whereas the postcoupling method for localizing active acid phosphatase gave equivocal results, the Gomori method for localizing the enzyme revealed that few of the perialgal vacuoles possesed ...


Mitochondrial Dna Of The Thatching Ant, Formica Obscuripes, Gregory Park Apr 1996

Mitochondrial Dna Of The Thatching Ant, Formica Obscuripes, Gregory Park

Life and Environmental Sciences Undergraduate Theses

In a continuing attempt to purify mitochondrial DNA from the thatching ant, modifications were made to a previously developed extraction procedure. Although mitochondria were highly enriched in a subcellular fraction derived from the heads of the ants, when DNA purified from the fraction by an alkaline lysis procedure was electrophoresed, inconsistent results were obtained. Bands in the gel show a molecule larger than 22 kilobase pairs, which is not expected of mtDNA.


A Comparative Study Of The Binding Of Two Lectins In The Early Development Of The Freshwater Sponge, Ephydatia Mulleri, Jay Taylor Apr 1996

A Comparative Study Of The Binding Of Two Lectins In The Early Development Of The Freshwater Sponge, Ephydatia Mulleri, Jay Taylor

Life and Environmental Sciences Undergraduate Theses

Carbohydrates have been implicated in cell adhesion and in developmental processes. Lectin histochemistry using ConcanavalinA and Lotus lectin was used to localize lectin-accessible mannosyl and fucosyl residues in a maturing sponge developing from histoblasts generated during germination of a gemmule. With the exception of cystencytes found in the sponges stained with Lotus lectin, a similar staining pattern was seen with the two lectins, but there was a rather significant difference in the intensity of staining. This difference in staining intensity was thought to be a result of either more mannosyl residues available to the lectin or that the concentration of ...


Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon Apr 1996

Preliminary Attempts To Purify Genomic And Mitochondrial Dna From A Freshwater Sponge, Derek Gordon

Life and Environmental Sciences Undergraduate Theses

This project was intended to be a pair of preliminary experiments from which more detailed and specific experiments could be expanded. There were two procedures used in carrying out this study involving the isolation of DNA from a freshwater sponge. First, genomic DNA was isolated and purified. This genomic DNA was digested with the restriction endonuclease EcoR1 and then analyzed using agarose gel electrophoresis. The results showed an accumulation of the sponge DNA in one major band roughly slightly larger than the 21226 bp band generated by EcoR1 digestion of X-phage DNA. The second part of this experiment was an ...


Concanavalin A-Binding Glycoconjugates In The Freshwater Sponge, Ephydatia Mulleri, Marek Janout Apr 1995

Concanavalin A-Binding Glycoconjugates In The Freshwater Sponge, Ephydatia Mulleri, Marek Janout

Life and Environmental Sciences Undergraduate Theses

This preliminary study investigated the distribution and role of cell surface carbohydrates which were suspected to affect proximal cell interactions such as recognition and adhesion. Recognition and adhesion play roles in the development of many organisms but are easily followed in freshwater sponges. The distribution of glycoconjugates in a freshwater sponge Ephydatia mulleri was investigated with the Periodic acid-Schiff (PAS) reaction and lectin histochemistry. PAS revealed the distribution of general carbohydrate in the sponge. Lectin histochemistry used Concanavalin A (ConA), a mannosebinding lectin, coupled through avidin and biotin to horseradish peroxidase enzyme (HRP) to reveal the location of mannose-containing glycoconjugates ...