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Full-Text Articles in Cell Biology

The Multifunctional Protein Daxx: Studies Of Its Biology And Regulation, And Discovery Of A Novel Function, Trisha Agrawal Jan 2013

The Multifunctional Protein Daxx: Studies Of Its Biology And Regulation, And Discovery Of A Novel Function, Trisha Agrawal

Publicly Accessible Penn Dissertations

Daxx, a multifunctional protein with a diverse set of proposed functions, is ubiquitously expressed and highly conserved through evolution. A primarily nuclear protein, Daxx is able to regulate apoptosis, transcription, and cellular proliferation. Despite many studies into the function of Daxx, its precise role in the cell remains enigmatic. Herein, evidence is presented to expand upon the known anti-apoptotic function of Daxx, to establish Daxx as a novel molecular chaperone, and to further its repertoire of transcriptional targets. As an apoptotic inhibitor, Daxx is known to regulate p53 by stabilizing its main negative regulator, Mdm2, via formation of a ternary ...


Regulation Of Tgf-Β1-Induced Apoptosis And Epithelial-Mesenchymal Transition By Matrix Rigidity, Jennifer L. Leight May 2011

Regulation Of Tgf-Β1-Induced Apoptosis And Epithelial-Mesenchymal Transition By Matrix Rigidity, Jennifer L. Leight

Publicly Accessible Penn Dissertations

Transforming growth factor-β (TGF-β) plays an important role in several diseases that characteristically involve changes in tissue rigidity, such as cancer and tissue fibrosis. To determine whether matrix rigidity regulates the effects of TGF-β, we examined NMuMG and MDCK epithelial cells cultured on polyacrylamide gels with varying rigidity and treated with TGF-β1. Decreasing matrix rigidity reduced cell spreading and increased TGF-β1-induced apoptosis, while increasing matrix rigidity resulted in epithelial-mesenchymal transition (EMT). To more carefully control cell spreading, microcontact printing was used to restrict ECM area and revealed that reducing cell spreading also increased apoptosis. Apoptosis on compliant substrates was associated ...