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2018

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Articles 1 - 30 of 35

Full-Text Articles in Cell Biology

Identification Of Deubiquitinating Enzymes That Control The Cell Cycle In Saccharomyces Cerevisiae, Claudine E. Mapa Nov 2018

Identification Of Deubiquitinating Enzymes That Control The Cell Cycle In Saccharomyces Cerevisiae, Claudine E. Mapa

GSBS Dissertations and Theses

A large fraction of the proteome displays cell cycle-dependent expression, which is important for cells to accurately grow and divide. Cyclical protein expression requires protein degradation via the ubiquitin proteasome system (UPS), and several ubiquitin ligases (E3) have established roles in this regulation. Less is understood about the roles of deubiquitinating enzymes (DUB), which antagonize E3 activity. A few DUBs have been shown to interact with and deubiquitinate cell cycle-regulatory E3s and their protein substrates, suggesting DUBs play key roles in cell cycle control. However, in vitro studies and characterization of individual DUB deletion strains in yeast suggest that these ...


Stochastic Difference Model For Evolutional Dynamics Of Large Antigen Repertoires In African Trypanosomes, Fan Yu Oct 2018

Stochastic Difference Model For Evolutional Dynamics Of Large Antigen Repertoires In African Trypanosomes, Fan Yu

Annual Symposium on Biomathematics and Ecology: Education and Research

No abstract provided.


Ire1b Degrades Rnas Encoding Proteins That Interfere With The Induction Of Autophagy By Er Stress In Arabidopsis Thaliana, Yan Bao, Yunting Pu, Xiang Yu, Brian D. Gregory, Renu Srivastava, Stephen H. Howell, Diane C. Bassham Aug 2018

Ire1b Degrades Rnas Encoding Proteins That Interfere With The Induction Of Autophagy By Er Stress In Arabidopsis Thaliana, Yan Bao, Yunting Pu, Xiang Yu, Brian D. Gregory, Renu Srivastava, Stephen H. Howell, Diane C. Bassham

Genetics, Development and Cell Biology Publications

Macroautophagy/autophagy is a conserved process in eukaryotes that contributes to cell survival in response to stress. Previously, we found that endoplasmic reticulum (ER) stress induces autophagy in plants via a pathway dependent upon AT5G24360/IRE1B (INOSITOL REQUIRING 1–1), an ER membrane-anchored factor involved in the splicing of AT1G42990/BZIP60 (basic leucine zipper protein 60) mRNA. IRE1B is a dual protein kinase and ribonuclease, and here we determined the involvement of the protein kinase catalytic domain, nucleotide binding and RNase domains of IRE1B in activating autophagy. We found that the nucleotide binding and RNase activity of IRE1B, but not ...


The Role Of Mesenchymal Stromal Cells And Classical Dendritic Cells In The Maintenance And Regulation Of The Bone Marrow Niche, Jingzhu Zhang Aug 2018

The Role Of Mesenchymal Stromal Cells And Classical Dendritic Cells In The Maintenance And Regulation Of The Bone Marrow Niche, Jingzhu Zhang

Arts & Sciences Electronic Theses and Dissertations

The bone marrow niche is an important microenvironment for the regulation of normal and malignant hematopoiesis. The first discovered niche component is mesenchymal stromal cells, which are the major source for the production and secretion of multiple niche factors. Mesenchymal stromal cells are heterogeneous and various transgenes have been used to target non-identical but overlapping subpopulations. To further characterize the heterogeneity of mesenchymal stromal cells, we tested the targeting specificity of three tissue-specific Cre-recombinase transgenes. We show that in addition to osteoblasts, Ocn-Cre targets a majority of Cxcl12-abundant reticular (CAR) cells and arteriolar pericytes. Surprisingly, Dmp1-Cre also targets a subset ...


Understanding Nuage-Mitochondrial Coupling In Drosophila Pirna Biogenesis, Tianfang Ge Aug 2018

Understanding Nuage-Mitochondrial Coupling In Drosophila Pirna Biogenesis, Tianfang Ge

GSBS Dissertations and Theses

In the Drosophilaovary, PIWI-interacting RNAs (piRNAs) suppress transposon expression, ensuring female fertility. PIWI proteins Aub and Ago3, loaded with ping-pong piRNAs and reside in perinuclear nuage granules, engage in reciprocal transposon transcript cleavage termed the ping-pong cycle. The other PIWI protein Piwi, loaded with phased piRNAs and resides in the nucleus, silences transposon transcriptionally. Ping-pong piRNAs are made through the ping-pong amplification loop by Aub and Ago3, whereas phased piRNAs are made through consecutive endonucleolytic cleavages that spread in 5′-to-3′ direction, presumably by Zucchini (Zuc), an endonuclease resides on the surface of mitochondria. The ping-pong and phasing biogenesis ...


Effects Of The Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, Maria Carparelli, Kelsey Ripley, Stefanie Otto-Hitt Jul 2018

Effects Of The Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, Maria Carparelli, Kelsey Ripley, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Titanium dioxide (TiO2) is a widely used compound found in everything from food packaging to sunscreens. When ingested, TiO2 is readily transported across membranes and efficiently stored within cells. Previous studies showed that exposure to TiO2 results in underdeveloped nervous systems. For our study, we attempted to answer the following question: Will exposing Drosophila melanogaster larvae to TiO2 affect expression of the Neur gene and development of the central nervous system? The Neur gene is crucial during the celldetermination stage of development as its encoded protein helps specify neuroblast development and aids in nervous system and sensory organ development. It ...


Effects Of Fatty Acids From Avocados On Expression Of The Hmg1 Gene In Tetrahymena Thermophila, Jasmine Phan, Connor Kane, Stefanie Otto-Hitt Jul 2018

Effects Of Fatty Acids From Avocados On Expression Of The Hmg1 Gene In Tetrahymena Thermophila, Jasmine Phan, Connor Kane, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The enzyme 3-Hydroxy-3-Methyl-Glutaryl-CoA Reductase (HMG) HMG catalyzes the ratelimiting step in the biosynthesis of cholesterol and is a common drug target for cholesterol reduction. Previous studies in rats have shown that avocado oil reduces cholesterol levels. We were interested in determining whether the healthy fatty acids in avocado oil would have an effect on the expression of HMG. In this study, Tetrahymena thermophila were treated with avocado oil to determine the effect of fatty acids on expression of the HMG1 gene that encodes HMG. Expression of HMG1 was monitored using reverse transcription and semi-quantitative PCR. We predicted that expression of ...


Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis, Stefanie Otto-Hitt Jul 2018

Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. For this experiment, it was hypothesized that alcohol induces starvation due to a lack of energy available in the organism for feeding. This hypothesis was tested by monitoring food vacuole formation and expression of the PFK1 gene in Tetrahymena that were exposed to alcohol. The PFK-1 gene was chosen because its encoded protein plays an essential role in cellular metabolism. For the experiment, control and experimental cultures of Tetrahymena themophila were maintained in a nutrient rich media, with the media of the experimental group ...


The Effect Of Exposure To Ionizing Radiation On Cwf16 Expression In Tetrahymena Thermophila, Shelby Olsen, David Woolston Jul 2018

The Effect Of Exposure To Ionizing Radiation On Cwf16 Expression In Tetrahymena Thermophila, Shelby Olsen, David Woolston

Life and Environmental Sciences Course-based Research Projects

The following research seeks to determine whether exposure to ultraviolet radiation will induce an increase in the expression of the Cwf16 gene in Tetrahymena thermophila. Cwf16 is a homolog of Ccgc94, whose gene product is a functioning member of the Prp19 complex, a protein complex which protects cells from committing apoptosisprogrammed cell deathdue to exposure to ionizing radiation. The homology between Cwf16 and Ccgc94 yields the hypothesis that Cwf16 expression in T. thermophila will increase in response to UV radiation exposure in a similar mechanism to that observed the Ccgc94 system. In the present research, T. thermophila cultures were randomly ...


The Effect Of Basic Conditions On Cdk1 Expression In Tetrahymena Thermophila, Kaitlin Stromberg, Jakob Plagenz, Stefanie Otto-Hitt Jul 2018

The Effect Of Basic Conditions On Cdk1 Expression In Tetrahymena Thermophila, Kaitlin Stromberg, Jakob Plagenz, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The goal of this project was to discover whether expression of the CDK1 gene in Tetrahymena thermophila, as well as their growth, are aected by changing the pH of the growth media. We hypothesized that expression of the CDK1 gene would decrease significantly when grown in basic media. The CDK1 gene encodes the protein Cyclin-Dependent Kinase, which functions to advance cells through various stages of the cell cycle. To test our hypothesis, Tetrahymena cultures were randomly assigned to either the control group, which was grown in media with a neutral pH, or a treatment group, which was grown in media ...


Effect Of Potassium Bromate On Oxr1 Gene Expression And Cell Growth In Tetrahymena Thermophila, Scott Khale, Brendan Mcmahon, Stefanie Otto-Hitt Jul 2018

Effect Of Potassium Bromate On Oxr1 Gene Expression And Cell Growth In Tetrahymena Thermophila, Scott Khale, Brendan Mcmahon, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The purpose of our experiment was to answer the question: Does oxidative damage, induced by potassium bromate (KBrO3), affect growth rate and expression of the OXR1 gene in Tetrahymena thermophila? Potassium Bromate was chosen as the environmental stressor because it is a strong oxidizing agent that is used as a leavening agent in baked goods. It was hypothesized that expression of OXR1 would increase in Tetrahymena cells that were exposed to potassium bromate and that their growth rate would decrease. The exact mechanism and function of the OXR1 gene is still unknown; however, the literature suggests that it is required ...


Effects Of Salt Concentration On Tetrahymena Thermophila Growth And Crp1 Gene Expression, Katie Mchugh, Rachel Tremaine, Anna Sapone, Stefanie Otto-Hitt Jul 2018

Effects Of Salt Concentration On Tetrahymena Thermophila Growth And Crp1 Gene Expression, Katie Mchugh, Rachel Tremaine, Anna Sapone, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The use of salt to remove ice from roads has resulted in increased salinity in many freshwater lakes in North America. For this project, we wanted to explore how environmental salt concentrations influence the rate of growth of Tetrahymena thermophila and expression of the CRP1 gene. We hypothesized that if the sodium concentration is increased in the media, Tetrahymena growth would decrease and expression of the CRP1 gene would increase. The CRP1 gene encodes a protein that helps regulate calcium concentrations within a cell based on the concentration of sodium ions. The media of the experimental group was treated with ...


The Effect Of Exposure To Uv Light On Rad51 Expression In Tetrahymena Thermophila, Kendall Patch, Kerri Mcinnis, Stefanie Otto-Hitt Jul 2018

The Effect Of Exposure To Uv Light On Rad51 Expression In Tetrahymena Thermophila, Kendall Patch, Kerri Mcinnis, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, we attempted to answer the question: Does exposure to UV light affect expressionof the Rad51 gene in Tetrahymena thermophila? It was hypothesized that the expression of Rad51 would increase proportionately to the exposure of UV light. The Rad51 protein is fundamental in the repair of double-stranded DNA breaks in eukaryotes, specifically during the process of homologous recombination. In order to test this hypothesis, Tetrahymena were randomly assigned to either a control group, which was cultured under ideal conditions, or a treatment group, which was exposed to UV light. Previous experiments have suggested that T. thermophila prosper most ...


Effect Of Nitrate Pollution On Tetrahymena Thermophila, Reegan Debruycker, Hannah Sylvester, Stefanie Otto-Hitt Jul 2018

Effect Of Nitrate Pollution On Tetrahymena Thermophila, Reegan Debruycker, Hannah Sylvester, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The question under investigation in this project was: Does an elevated concentration of nitrates have a negative eect on cell growth and expression of the CDC16 gene in the organism Tetrahymena thermophila? Exposure of Tetrahymena cultures to nitrates mimics the exposure of these organisms to nitrate pollution in the environment, which can occur due to septic tank leakage, nitrogen-rich fertilizer run o, and agricultural processes. The EPA standard for nitrate concentrations in drinking water is a maximum of 10ppm. In our experiment, Tetrahymena cultures were treated with media that contained 30ppm of nitrates for one week. The 30ppm nitrate concentration ...


Impacts Of Environmental Motion On Cdc2 Expression And Cell Growth In Tetrahymena Thermophila, Jack Rees, Keaton Brady, Stefanie Otto-Hitt Jul 2018

Impacts Of Environmental Motion On Cdc2 Expression And Cell Growth In Tetrahymena Thermophila, Jack Rees, Keaton Brady, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, the eect of constant motion on expression of the cell cycle control gene CDC2 was examined in the organism Tetrahymena thermophila. We hypothesized that CDC2 expression would decrease with a corresponding increase in movement within the Tetrahymena culture environment. The CDC2 protein is primarily used in the replication of eukaryotic DNA because of its connection to HHO1, an important factor in transcriptional regulation. If the cells were subject to vegetative growth in which there are no stressors put on the cell, then HHO1 becomes phosphorylated to prevent it from interacting with chromatin. Because CDC2 is important in ...


Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt Jul 2018

Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Scramblase is an enzyme that facilitates the movement of newly synthesized phospholipids from the cytosolic side to the extracellular side of the lipid bilayer. This process is vital for cell membrane repair and growth. In Tetrahymena thermophila, the gene SIT1 encodes for the Scramblase protein, whose functionality is Ca2+-dependent. In this experiment, the concentration of accessible Ca2+ ions was decreased in order to observe whether the change had an aect on the expression of SIT1 and cell growth. It was hypothesized that expression of the SIT1 gene would increase, while cell growth would decrease. To carry out the experiment ...


Analysis Of Fbp1 Gene Expression In Uv Light-Exposed Tetrahymena Thermophila Cultures, Colby Henry, Stefanie Otto-Hitt Jul 2018

Analysis Of Fbp1 Gene Expression In Uv Light-Exposed Tetrahymena Thermophila Cultures, Colby Henry, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this study, Tetrahymena thermophila cultures were exposed to an acute treatment of UV light and expression of the FBP1 gene was examined. The FBP1 gene encodes Fructose- 1,6-biphosphatase which plays an important role in the process of gluconeogenesis. It was hypothesized that exposure of Tetrahymena to UV light would cause an upregulation in DNA repair enzymes, thereby causing these cells to require higher levels of glucose. The higher demand for glucose would then result in the upregulation of gluconeogenesis and FBP1. Following acute exposure of Tetrahymena cultures to UV light, the expression of FBP1 was analyzed using reverse ...


Exploring The Effects Of Environmental Temperature On Cdc-7 Expression In T. Thermophila, John Brothers, Alexis Gerry, Lauren Scofield, Stefanie Otto-Hitt Jul 2018

Exploring The Effects Of Environmental Temperature On Cdc-7 Expression In T. Thermophila, John Brothers, Alexis Gerry, Lauren Scofield, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Tetrahymena thermophila are unicellular eukaryotes with a remarkably large genome: over 27,000 genes. One important aspect of studying the Tetrahymena genome is identifying and understanding what factors influence gene expression. This study examined the effects of temperature on the expression of CDC-7, a cell division control gene. We subjected Tetrahymena cultures to increased temperature and hypothesized such treatments would induce cell division and increase CDC-7 expression. Gene expression levels were measured via semi-quantitative PCR analysis after an acute heat treatment.


Uv Light Exposure And Tetrahymena Thermophilia: Expression Of The Phr2 Gene, Molly Moloney, Tanner Gustavsen, Stefanie Otto-Hitt Jul 2018

Uv Light Exposure And Tetrahymena Thermophilia: Expression Of The Phr2 Gene, Molly Moloney, Tanner Gustavsen, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

The PHR2 (photolyase repair) gene functions in the repair of DNA in Tetrahymena thermophila. It was hypothesized that when Tetrahymena cultures are exposed to UV light, increased expression of PHR2 would occur in response to the damage incurred. UV light has been known to cause the formation of pyrimidine dimers and other disruptions in DNA. Through two rounds of experimentation, the acute impact of UV light exposure was monitored. The cultures underwent RNA extraction, reverse transcription, and semi-quantitative PCR analysis. We predicted that UV exposure would result in the increased expression of PHR2.


The Effects Of Warming Temperatures On Rad51 Expression In Tetrahymena Thermophila, Timothy Brunson, Rachael Lefstad, Stefanie Otto-Hitt Jul 2018

The Effects Of Warming Temperatures On Rad51 Expression In Tetrahymena Thermophila, Timothy Brunson, Rachael Lefstad, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Our experiment explored whether raised temperatures affected expression of the Rad51 gene in Tetrahymena thermophila. Our hypothesis was that raised temperatures would result in a decrease in expression of the Rad51 gene. The protein product of this gene functions in double stranded DNA repair. We chose this experiment to see if the rising average temperatures around the world would affect expression of genes involved in DNA repair. Our test samples were kept in a growth medium at 37C while our control samples were kept in a growth medium at 24C. Afterwards, the transcribed mRNA was reverse transcribed into DNA and ...


Analyzing Atp Synthetase F1 Expression In Heat-Exposed Tetrahymena Thermophila, Kevin Gardner, Ian Lorang, Stefanie Otto-Hitt Jul 2018

Analyzing Atp Synthetase F1 Expression In Heat-Exposed Tetrahymena Thermophila, Kevin Gardner, Ian Lorang, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this study, we investigated the effect of temperature on expression of the ATP1 gene in the organism Tetrahymena thermophila. We hypothesized that expression of the ATP1 gene would increase as we increased temperature. ATP1 is a highly conserved gene in Tetrahymena that codes for the alpha subunit of ATP Synthase, an enzyme that generates the energy molecule ATP. We decided to use heat treatment based on studies performed on ATP1 in Alaskan Cod showing increased expression in response to increased temperatures (Michael et al., 2015). We used reverse transcription and semi-quantitative PCR to analyze expression of ATP1 in our ...


The Effect Of Metal Pollutants On Cell Growth And Ttherm0058490expressionint Etrahymenathermophila, Inderbir Bains, William French, Stefanie Otto-Hitt Jul 2018

The Effect Of Metal Pollutants On Cell Growth And Ttherm0058490expressionint Etrahymenathermophila, Inderbir Bains, William French, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

In this experiment, we sought to answer the following question: Do higher levels of metal exposure affect expression of the TTHERM 00584900 gene in Tetrahymena thermophila? The TTHERM 00584900 gene produces an ATP-binding cassette protein that is responsible for removing metals and metalloids from Tetrahymena thermophila. It is hypothesized that the expression of TTHERM 00584900 will increase with exposure to metals. In order to test this hypothesis, Tetrahymena thermophila were randomly assigned to either a control group, which was cultured in a normal growth medium, or a treatment group, whichin addition to the medium was exposed to water contaminated with ...


Exploring The Role Of Rna Polymerase Iii Complex Assembly On Ribosomal Dna Silencing In Saccharomyces Cerevisiae, Kyle Thomas Kern Jun 2018

Exploring The Role Of Rna Polymerase Iii Complex Assembly On Ribosomal Dna Silencing In Saccharomyces Cerevisiae, Kyle Thomas Kern

LSU Doctoral Dissertations

The yeast rDNA region is host to a number of transcriptional regulatory elements, which work in conjunction to generate essential RNA subunits of ribosomes, as well as protecting the region from DNA damage. The role of RNA polymerase III complex binding at the 5S gene on rDNA silencing in the NTS2 region was investigated, both by use of a TY1:MET15 reporter insert and a MET15 gene integration at an endogenous SphI site. It was discovered that Pol III complexes do have an effect on reporter expression in the NTS2 region, though the specific effect was different based on the ...


Tarbp2 -Mediated Post-Transcriptional Regulation Of Gene Expression During Murine Embryonic Development And Spermatogenesis, Sri Ramulu N. Pullagura May 2018

Tarbp2 -Mediated Post-Transcriptional Regulation Of Gene Expression During Murine Embryonic Development And Spermatogenesis, Sri Ramulu N. Pullagura

Electronic Theses and Dissertations

Micro RNAs (miRNAs), which are ~22 nucleotide (nt) long RNA molecules and several RNA binding proteins (RBPs) engage in an RNA dependent post-transcriptional gene silencing process known as RNA interference (RNAi). In the canonical miRNA biogenesis pathway, an enzyme known as DICER cleaves the ~70nt pre-miRNA to a ~22nt long miRNA that is loaded into the RNAi effector mechanism, the RNA induced silencing complex (RISC).

Several in vitro studies provide suggestive evidence that mammalian double stranded RNA binding proteins (dsRBPs), such as TARBP2, act as DICER cofactors in miRNA processing and RISC loading to promote RNAi activity. A screen attempting ...


Functional Studies Of The E. Coli Proc And A Putative Ortholog Mrub_1345, Mourin Azar, Dr. Lori Scott May 2018

Functional Studies Of The E. Coli Proc And A Putative Ortholog Mrub_1345, Mourin Azar, Dr. Lori Scott

Meiothermus ruber Genome Analysis Project

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of Escherichia coli and Meiothermus ruber proC genes using the complementation assay. In this research project, mutants of varying severity to the functional state of the protein were developed. The results showed that two or more amino acid deletions reduced or eliminated ProC function. Amino acid substitutions, on the other hand, were not severe enough to impact ProC function. Double and triple mutants ...


Evolutionary Conservation Of Midline Repulsion By Robo Family Receptors In Flies And Mice, Allison Loy May 2018

Evolutionary Conservation Of Midline Repulsion By Robo Family Receptors In Flies And Mice, Allison Loy

Biological Sciences Undergraduate Honors Theses

As the nervous system develops in animal embryos, neuronal axons are guided to their synaptic targets by extra cellular cues that signal through axon guidance receptors expressed on the surface of the axon. In animals with bilateral symmetry, one of the important decisions made by nearly every axon in the embryonic nervous system is whether to stay on its own side of the body, or to cross the midline and connect to cells on the opposite side. The Roundabout (Robo) family is an evolutionarily conserved group of axon guidance receptors that regulate midline crossing in a wide range of animal ...


Restoration Of Phage Growth On A Non-Permissive Host By Bypassing Transcription Termination Signals, Millicent Ronkainen Apr 2018

Restoration Of Phage Growth On A Non-Permissive Host By Bypassing Transcription Termination Signals, Millicent Ronkainen

Honors College Capstone Experience/Thesis Projects

RNA polymerase is the central enzyme in all gene expression. The rpoCY75N mutation in the zinc-binding domain of the β’ subunit of E. coli RNA polymerase blocks a unique RNA-based mechanism of transcription antitermination utilized by bacteriophage HK022 and its relatives. Here, we describe the characterization of mutant phage, orc0368, which overcomes the rpoCY75N mutation. The orc0368 genome varies from the wild type phage genome by 4 single base pair mutations. Three of these mutations were not characterized because they occur in intergenic regions but the fourth was chosen for study because of its location between a series ...


Characterization Of A Morphogenetic Furrow Specific Gal4 Driver In The Developing Drosophila Eye, Ankita Sarkar, Neha Gogia, Kevin Farley, Lydia C. Payton, Amit Singh Apr 2018

Characterization Of A Morphogenetic Furrow Specific Gal4 Driver In The Developing Drosophila Eye, Ankita Sarkar, Neha Gogia, Kevin Farley, Lydia C. Payton, Amit Singh

Biology Faculty Publications

The ability to express a gene of interest in a spatio-temporal manner using Gal4-UAS system has allowed the use of Drosophila model to study various biological phenomenon. During Drosophila eye development, a synchronous wave of differentiation called Morphogenetic furrow (MF) initiates at the posterior margin resulting in differentiation of retinal neurons. This synchronous differentiation is also observed in the differentiating retina of vertebrates. Since MF is highly dynamic, it can serve as an excellent model to study patterning and differentiation. However, there are not any Gal4 drivers available to observe the gain- of- function or loss- of- function of a ...


Differential Gene Expression In Response To Hypoxia And Acidosis In Chest Wall Deformities And Chondrosarcoma, Jamie L. Durbin Apr 2018

Differential Gene Expression In Response To Hypoxia And Acidosis In Chest Wall Deformities And Chondrosarcoma, Jamie L. Durbin

Biological Sciences Theses & Dissertations

The importance of understanding how costal cartilage chondrocytes respond to stimuli such as oxidative stress and low pH has been largely overlooked in studies involving tissue culturing due to major differences between oxygen and pH levels during incubation and the natural environment of hyaline cartilage. Hyaline cartilage is avascular and naturally hypoxic which subsequently leads to increased glycolytic metabolism and ultimately causes a decrease in extracellular pH. To examine how healthy costal cartilage responds to these extreme growth conditions, we examined responses in three hyaline cartilage diseases. Our ability to identify the disease mechanisms responsible for pectus excavatum, pectus carinatum ...


Atypical Pkciota Contributes To Poor Prognosis Through Loss Of Apical-Basal Polarity And Cyclin E Overexpression In Ovarian Cancer, Astrid M. Eder, Xiaomei Sui, Daniel G. Rosen, Laura K. Nolden, Kwai Wa Cheng, John P. Lahad, Madhuri Kango-Singh, Karen H. Lu, Carla L. Warneke, Edward N. Atkinson, Isabelle Bedrosian, Khandan Keyomarsi, Wen-Lin Kuo, Joe W. Gray, Jerry C. P. Yin, Jinsong Liu, Georg Halder, Gordon B. Mills Feb 2018

Atypical Pkciota Contributes To Poor Prognosis Through Loss Of Apical-Basal Polarity And Cyclin E Overexpression In Ovarian Cancer, Astrid M. Eder, Xiaomei Sui, Daniel G. Rosen, Laura K. Nolden, Kwai Wa Cheng, John P. Lahad, Madhuri Kango-Singh, Karen H. Lu, Carla L. Warneke, Edward N. Atkinson, Isabelle Bedrosian, Khandan Keyomarsi, Wen-Lin Kuo, Joe W. Gray, Jerry C. P. Yin, Jinsong Liu, Georg Halder, Gordon B. Mills

Madhuri Kango-Singh

We show that atypical PKCι, which plays a critical role in the establishment and maintenance of epithelial cell polarity, is genomically amplified and overexpressed in serous epithelial ovarian cancers. Furthermore, PKCι protein is markedly increased or mislocalized in all serous ovarian cancers. An increased PKCι DNA copy number is associated with decreased progression-free survival in serous epithelial ovarian cancers. In a Drosophila in vivo epithelial tissue model, overexpression of persistently active atypical PKC results in defects in apical-basal polarity, increased Cyclin E protein expression, and increased proliferation. Similar to the Drosophila model, increased PKCι proteins levels are associated with ...