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Full-Text Articles in Cell Biology

Common-Path Multimodal Optical Microscopy, Marla Tipping, Chandra S. Yelleswarapu, Sri-Rajasekhar Kothapalli, Alexey Veraksa, D.V.G.L.N. Rao Jun 2014

Common-Path Multimodal Optical Microscopy, Marla Tipping, Chandra S. Yelleswarapu, Sri-Rajasekhar Kothapalli, Alexey Veraksa, D.V.G.L.N. Rao

Marla Tipping, Ph.D.

We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield + fluorescence, phase +fluorescence, and edge-enhanced+fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of ...


Signaling Functions Of Beta-Arrestin Kurtz In Drosophila Development, Marla Tipping Jun 2014

Signaling Functions Of Beta-Arrestin Kurtz In Drosophila Development, Marla Tipping

Marla Tipping, Ph.D.

The development of an organism is orchestrated by a small number of signaling pathways. In order to achieve tissue specificity, their activities are controlled by multiple regulatory molecules that fine-tune pathway output and increase the specificity of signaling. To better understand how these pathways function it is imperative that we strive to elucidate all signaling components. Affinity purification followed by Mass Spectrometry (AP-MS) has proved itself as a suitable technique in achieving this aim. Analysis of the Notch pathway via this method has identified Drosophila β-arrestin Kurtz (Krz) as a negative modulator of the pathway. I have further studied Krz ...