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2002

Animals; Cell Division; Cell Line; Fluorescence Recovery After Photobleaching; Immunohistochemistry; Microtubules; Protein Transport; Protein-Serine-Threonine Kinases; Recombinant Fusion Proteins; Signal Transduction

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Full-Text Articles in Cell Biology

Both Midzone And Astral Microtubules Are Involved In The Delivery Of Cytokinesis Signals: Insights From The Mobility Of Aurora B, Maki Murata-Hori, Yu-Li Wang Oct 2002

Both Midzone And Astral Microtubules Are Involved In The Delivery Of Cytokinesis Signals: Insights From The Mobility Of Aurora B, Maki Murata-Hori, Yu-Li Wang

Open Access Articles

To address the mechanism that coordinates cytokinesis with mitosis, we have studied the dynamics of aurora B, a chromosomal passenger protein involved in signaling cytokinesis. Photobleaching analyses indicated dynamic exchange of aurora B between a centromeric and a cytoplasmic pool before anaphase onset, and stable associations with microtubules after anaphase onset. Bleaching near centromeres upon anaphase onset affected the subsequent appearance of fluorescence along midzone microtubules, but not that near the lateral equatorial cortex, suggesting that there were centromeric-dependent and -independent pathways that transported aurora B to the equator. The former delivered centromeric aurora B along midzone microtubules, whereas the ...