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Full-Text Articles in Cell Biology

Molecular Interactions Of Talin And Actin: A Study On The Specific Domains Of Talin Involved In The Interactions , Ho-Sup Lee Jan 2002

Molecular Interactions Of Talin And Actin: A Study On The Specific Domains Of Talin Involved In The Interactions , Ho-Sup Lee

Retrospective Theses and Dissertations

Talin is an essential cytoskeletal protein present in cell-matrix type adherens junctions where it helps in attachment of the actin cytoskeleton to the plasma membrane. The nature of the interaction between talin and actin was examined in this dissertation by investigating three actin binding sites (ABS1--3), with ABS1 located in the 47 kDa N-terminal head and ABS2 and ABS3 present in the 190 kDa C-terminal tail of the talin molecule. Cosedimentation of expressed GST-fusion deletion constructs from the talin head with F-actin showed that actin binding activity of the talin head is contained within residues 271--320. Talin constructs containing the ...


Nitric Oxide In Astrocyte-Neuron Signaling , Nianzhen Li Jan 2002

Nitric Oxide In Astrocyte-Neuron Signaling , Nianzhen Li

Retrospective Theses and Dissertations

Astrocytes, a subtype of glial cell, have recently been shown to exhibit Ca2+ elevations in response to neurotransmitters. A Ca 2+ elevation can propagate to adjacent astrocytes as a Ca2+ wave, which allows an astrocyte to communicate with its neighbors. Additionally, glutamate can be released from astrocytes via a Ca2+ -dependent mechanism, thus modulating neuronal activity and synaptic transmission.;In this dissertation, I investigated the roles of another endogenous signal, nitric oxide (NO), in astrocyte-neuron signaling. First I tested if NO is generated during astrocytic Ca2+ signaling by imaging NO in purified murine cortical astrocyte cultures. Physiological concentrations of a ...