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2002

Department of Microbiology Papers

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Full-Text Articles in Cell Biology

The D0 Domain Of Kir3d Acts As A Major Histocompatibility Complex Class I Binding Enhancer, Salim I. Khakoo, Ron Geller, Sunny Shin, Jomaquai A. Jenkins, Peter Parham Oct 2002

The D0 Domain Of Kir3d Acts As A Major Histocompatibility Complex Class I Binding Enhancer, Salim I. Khakoo, Ron Geller, Sunny Shin, Jomaquai A. Jenkins, Peter Parham

Department of Microbiology Papers

In contrast to the KIR2D:HLA-C interaction, little is known of KIR3DL1's interaction with HLA-B or the role of D0, the domain not present in KIR2D. Differences in the strength and specificity for major histocompatibility complex class I of KIR3DL1 and its common chimpanzee homologue Pt-KIR3DL1/2 were exploited to address these questions. Domain-swap, deletion, and site-directed mutants of KIR3DL1 were analyzed for HLA-B binding using a novel, positively signaling cell–cell binding assay. Natural ‘deletion’ of residues 50 and 51 from its D0 domain causes Pt-KIR3DL1/2 to bind Bw4+ HLA-B allotypes more avidly than does KIR3DL1. Deletion ...