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2002

University of Massachusetts Medical School

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Articles 1 - 5 of 5

Full-Text Articles in Cell Biology

Both Midzone And Astral Microtubules Are Involved In The Delivery Of Cytokinesis Signals: Insights From The Mobility Of Aurora B, Maki Murata-Hori, Yu-Li Wang Oct 2002

Both Midzone And Astral Microtubules Are Involved In The Delivery Of Cytokinesis Signals: Insights From The Mobility Of Aurora B, Maki Murata-Hori, Yu-Li Wang

Open Access Articles

To address the mechanism that coordinates cytokinesis with mitosis, we have studied the dynamics of aurora B, a chromosomal passenger protein involved in signaling cytokinesis. Photobleaching analyses indicated dynamic exchange of aurora B between a centromeric and a cytoplasmic pool before anaphase onset, and stable associations with microtubules after anaphase onset. Bleaching near centromeres upon anaphase onset affected the subsequent appearance of fluorescence along midzone microtubules, but not that near the lateral equatorial cortex, suggesting that there were centromeric-dependent and -independent pathways that transported aurora B to the equator. The former delivered centromeric aurora B along midzone microtubules, whereas the ...


The Cyclin-Dependent Kinase 5 Activators P35 And P39 Interact With The Alpha-Subunit Of Ca2+/Calmodulin-Dependent Protein Kinase Ii And Alpha-Actinin-1 In A Calcium-Dependent Manner, Rani Dhavan, Paul L. Greer, Maria A. Morabito, Lianna R. Orlando, Li-Huei Tsai Sep 2002

The Cyclin-Dependent Kinase 5 Activators P35 And P39 Interact With The Alpha-Subunit Of Ca2+/Calmodulin-Dependent Protein Kinase Ii And Alpha-Actinin-1 In A Calcium-Dependent Manner, Rani Dhavan, Paul L. Greer, Maria A. Morabito, Lianna R. Orlando, Li-Huei Tsai

Morabito Lab Publications

Cyclin-dependent kinase 5 (Cdk5) is a critical regulator of neuronal migration in the developing CNS, and recent studies have revealed a role for Cdk5 in synaptogenesis and regulation of synaptic transmission. Deregulation of Cdk5 has been linked to the pathology of neurodegenerative diseases such as Alzheimer's disease. Activation of Cdk5 requires its association with a regulatory subunit, and two Cdk5 activators, p35 and p39, have been identified. To gain further insight into the functions of Cdk5, we identified proteins that interact with p39 in a yeast two-hybrid screen. In this study we report that alpha-actinin-1 and the alpha-subunit of ...


The Intraflagellar Transport Protein, Ift88, Is Essential For Vertebrate Photoreceptor Assembly And Maintenance, Gregory J. Pazour, Sheila A. Baker, James A. Deane, Douglas G. Cole, Bethany L. Dickert, Joel L. Rosenbaum, George B. Witman, Joseph C. Besharse Mar 2002

The Intraflagellar Transport Protein, Ift88, Is Essential For Vertebrate Photoreceptor Assembly And Maintenance, Gregory J. Pazour, Sheila A. Baker, James A. Deane, Douglas G. Cole, Bethany L. Dickert, Joel L. Rosenbaum, George B. Witman, Joseph C. Besharse

Open Access Articles

Approximately 10% of the photoreceptor outer segment (OS) is turned over each day, requiring large amounts of lipid and protein to be moved from the inner segment to the OS. Defects in intraphotoreceptor transport can lead to retinal degeneration and blindness. The transport mechanisms are unknown, but because the OS is a modified cilium, intraflagellar transport (IFT) is a candidate mechanism. IFT involves movement of large protein complexes along ciliary microtubules and is required for assembly and maintenance of cilia. We show that IFT particle proteins are localized to photoreceptor connecting cilia. We further find that mice with a mutation ...


Fc-Receptor-Mediated Phagocytosis Is Regulated By Mechanical Properties Of The Target, Karen A. Beningo, Yu-Li Wang Feb 2002

Fc-Receptor-Mediated Phagocytosis Is Regulated By Mechanical Properties Of The Target, Karen A. Beningo, Yu-Li Wang

Open Access Articles

Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. It is commonly assumed that the recognition/selection of phagocytic targets is based solely upon receptor-ligand binding. Here we report an important new criterion, that mechanical parameters of the target can dramatically affect the efficiency of phagocytosis. When presented with particles of identical chemical properties but different rigidity, macrophages showed a strong preference to engulf ...


Evidence That The Rat Osteopetrotic Mutation Toothless (Tl) Is Not In The Tnfsf11 (Trance, Rankl, Odf, Opgl) Gene, Paul R. Odgren, Nacksung Kim, Liesbeth Van Wesenbeeck, Carole A. Mackay, April Mason-Savas, Fayez F. Safadi, Steven N. Popoff, Christopher J. Lengner, Wim Van-Hul, Yongwon Choi, Sandy C. Marks Jr. Jan 2002

Evidence That The Rat Osteopetrotic Mutation Toothless (Tl) Is Not In The Tnfsf11 (Trance, Rankl, Odf, Opgl) Gene, Paul R. Odgren, Nacksung Kim, Liesbeth Van Wesenbeeck, Carole A. Mackay, April Mason-Savas, Fayez F. Safadi, Steven N. Popoff, Christopher J. Lengner, Wim Van-Hul, Yongwon Choi, Sandy C. Marks Jr.

Open Access Articles

The toothless (tl) osteopetrotic mutation in the rat affects an osteoblast-derived factor that is required for normal osteoclast differentiation. Although the genetic locus remains unknown, the phenotypic impact of the tl mutation on multiple systems has been well characterized. Some of its actions are similar to tumornecrosis factor superfamily member 11(TNFSF11; also called TRANCE, RANKL, ODF and OPGL) null mice. TNFSF11 is a recently described member of the tumor necrosis factor superfamily which, when expressed by activated T cells, enhances the survival of antigen-presenting dendritic cells, and when expressed by osteoblasts, promotes the differentiation and activation of osteoclasts. The ...