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Full-Text Articles in Cell Biology

#2 - Comparison Of Ion Transport Expression In Lake Sturgeon Exposed To Differing Water Chemistries, Caitlin Forkin Nov 2019

#2 - Comparison Of Ion Transport Expression In Lake Sturgeon Exposed To Differing Water Chemistries, Caitlin Forkin

Georgia Undergraduate Research Conference (GURC)

This project investigated ion uptake by fish endemic to Georgia, known as Lake Sturgeon (Acipenser fulvescens), which were acquired from a hatchery and naturally found in the Coosa River. For this investigation, identical recirculating tank systems provided separated environments for the two types of water treatments. Equal test groups were placed into the different water chemistries, one into the Coosa River and the other into the regular dechlorinated tap water. All subjects consumed the same type of food: Rangen, which is a commercial chow mix, and blood worm. The Coosa River water differs in ionic composition compared to the hatchery ...


#3 - Generation Of Sult4a1 Gene Mutations In Sh-Sy5y Cells, Elisabeth Bradberry, Frank Crittenden Nov 2019

#3 - Generation Of Sult4a1 Gene Mutations In Sh-Sy5y Cells, Elisabeth Bradberry, Frank Crittenden

Georgia Undergraduate Research Conference (GURC)

The cytosolic sulfotransferases (SULT) are a superfamily of enzymes that catalyze the metabolism of various substrates throughout the body. One member, SULT4A1, has no known substrates and is highly conserved among all vertebrates which is not a shared characteristic among the SULT family. Also unique among the SULTs, SULT4A1 localizes with mitochondria of neurons. Recent reports have suggested that this protein is believed play a protective role against oxidative stress. The goal of this project was to generate a SH-SY5Y cell line with a SULT4A1 gene deletion using CRISPR gene-editing technology. These neuroblastoma cells were used because of their ease ...


#7 - The Role Of Rnf216/Triad3 In Neuroinflammation Through Interactions With Toll-Like-Receptors, Dustin Grossman Nov 2019

#7 - The Role Of Rnf216/Triad3 In Neuroinflammation Through Interactions With Toll-Like-Receptors, Dustin Grossman

Georgia Undergraduate Research Conference (GURC)

Ubiquitin E3 ligases are enzymes that mark certain substrates with ubiquitin proteins which leads to different cellular fates. Ring finger protein 216 (RNF216) is a ubiquitin E3 ligase that is involved in synaptic plasticity, inhibiting cellular autophagy, and the immune response in the peripheral nervous system. Previous literature has demonstrated that RNF216 participates in various aspects of inflammation by regulating ubiquitination and proteasomal degradation of receptor-interacting serine-threonine kinase 1 (RIPK1), toll-interleukin 1 receptor domain containing adaptor protein (TIRAP), and TIR-domain-containing adapter-inducing interferon-β (TRIF), targeting TNF receptor associated factor 3 (TRAF3) for degradation. TLRs initiate signal transduction pathways which can lead ...


Development Of Fret Biosensors To Detect Kinase Activity In Living Cells, Jenny Okáľová Nov 2019

Development Of Fret Biosensors To Detect Kinase Activity In Living Cells, Jenny Okáľová

Georgia Undergraduate Research Conference (GURC)

Development of FRET Biosensors to Detect Kinase Activity in Living Cells
Jenny Okáľová, Undergraduate Research Assistant and CURO Honors Scholar
Dr. Neil J. Grimsey, Pharmaceutical and Biomedical Sciences, College of Pharmacy

G-protein-coupled receptors (GPCRs) are a group of over 800 membrane receptors that induce intracellular signaling cascades to interpret an array of external signals. The p38 mitogen-activated protein kinase (MAPK) pathway plays a key role in inflammatory responses found in many diseases. Our group discovered a family of GPCRs that regulate an atypical pathway for p38 activation in vascular cells. This atypical pathway specifically requires transforming growth factor-β (TGFβ) activated ...


Thymic Epithelial Cell Reprogramming And Co-Culture With T-Cell Progenitors, Isabelle Riddle Nov 2018

Thymic Epithelial Cell Reprogramming And Co-Culture With T-Cell Progenitors, Isabelle Riddle

Georgia Undergraduate Research Conference (GURC)

The thymus is a lymphoid organ found in the neck region of vertebrates that produces T-cells critical for the adaptive immune system. However, thymus involution relatively early in the aging process leads to a deterioration in immune function. Models in regenerative medicine have the prospect of replacing lost cells and combating the problem. This experiment is a preliminary step geared towards achieving the overarching goal of boosting long-term maintenance of adaptive immunity over the typical aging process or following therapeutic immune depletion. Specifically, the objective is to show that disparate cell lineages can be reprogrammed—both morphologically and physiologically—using ...