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Articles 1 - 4 of 4

Full-Text Articles in Cell Biology

The Effects Of Climate Change On Basic Animal Cell Functions, Michelle Fulbright, Andrew Andres Apr 2011

The Effects Of Climate Change On Basic Animal Cell Functions, Michelle Fulbright, Andrew Andres

Festival of Communities: UG Symposium (Posters)

Increasing global temperatures during the 21st century may have detrimental effects on basic cell functions within ectothermal animals. This project aims to systematically examine the effects of climate change on secretory cargo localization through the analysis of Rab Proteins found in the model organism Drosophila melanogaster. Rab Proteins play an essential role in vesicular transport within the cell and can be genetically manipulated to monitor the biological consequences of global warming.


Stationary Phase Mutagenesis In Bacillus Subtilisis Independent Of Genome Replication, Mark Upchurch, Holly Martin, Eduardo Robleto Apr 2011

Stationary Phase Mutagenesis In Bacillus Subtilisis Independent Of Genome Replication, Mark Upchurch, Holly Martin, Eduardo Robleto

Festival of Communities: UG Symposium (Posters)

Stationary phase mutagenesis is defined as cellular mechanisms that produce genetic diversity in cells experiencing conditions of stress. These processes are associated with many biological phenomena, including those that produce the formation of cancers in animal cells and other degenerative diseases. Also, these mechanisms are associated with the accumulation of beneficial mutations in bacteria, but follow stochastic processes and are controlled by genetic factors. The current models explaining the generation of stress-induced mutations are predicated on the formation of DNA replication intermediates that are formed during the repair of damaged DNA or during DNA replication and transcription encounters. Here we ...


The Release Of Calcium In Bacillus Anthracis Pathogenicity Methods, Natiera Magnuson, Manomita Patra Bhowmik, Maria Elena Reynaga, Ernesto Abel-Santos Apr 2011

The Release Of Calcium In Bacillus Anthracis Pathogenicity Methods, Natiera Magnuson, Manomita Patra Bhowmik, Maria Elena Reynaga, Ernesto Abel-Santos

Festival of Communities: UG Symposium (Posters)

Anthrax infection starts with germination of Bacillus anthracis spores in macrophages. Some bacteria, including B. anthracis, can sporulate in response to environmental stress, such as starvation. During germination, large concentrations of calcium ions are released from the B. anthracis spore. Calcium ions are hydrophilic secondary messengers, and may therefore interfere with detection of the spore by confusing the cell signaling pathways. We investigated calcium release on infected macrophage viability by replacing the calcium stored in B. anthracis spores for other cations via demineralization/remineralization. It was discovered that calcium ions typically out-performed other cations in germination of B. anthracis.


Stationary Phase Mutagenesis In Bacillus Subtilis: The Interaction Between Transcription And Error-Prone Replication In Conditions Of Stress, Mary Girard, Eduardo Robleto Aug 2009

Stationary Phase Mutagenesis In Bacillus Subtilis: The Interaction Between Transcription And Error-Prone Replication In Conditions Of Stress, Mary Girard, Eduardo Robleto

Undergraduate Research Opportunities Program (UROP)

While under conditions of stress, non-dividing cells may acquire beneficial mutations. This is referred to as stationary phase mutagenesis, or adaptive mutagenesis. Previous research has shown that actively transcribed genes and those under selective pressure are prone to mutations that confer escape from non-dividing conditions. Accordingly, strains lacking transcription factors have shown a drastically lower number of mutations that confer escape while under amino acid starvation than those observed in the wildtype background. Also, error-prone DNA polymerases are known to be active in cells under stress and it has been shown that strains lacking an error-prone DNA polymerase display reduced ...