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Full-Text Articles in Cell Biology

Calibrated Brightfield-Based Imaging For Measuring Intracellular Protein Concentration, Nathan Mudrak, Priyanka S. Rana, Michael A. Model Mar 2017

Calibrated Brightfield-Based Imaging For Measuring Intracellular Protein Concentration, Nathan Mudrak, Priyanka S. Rana, Michael A. Model

Undergraduate Research Symposium

Intracellular protein concentration is an essential cell characteristic which manifests itself through the refractive index. The latter can be measured from two or more mutually defocused brightfield images analyzed using the TIE (transport-of-intensity equation). In practice, however, TIE does not always achieve quantitatively accurate results on biological cells. Therefore, we have developed a calibration procedure that involves successive imaging of cells in solutions containing different amounts of added protein. This allows one to directly relate the output of TIE (T) to intracellular protein concentration C (g/l). The resultant relationship has a simple form: C ≈ 1.0(T/V), where ...


Peptide Inhibitors That Disrupt Viral-Viral Protein Interactions In Hiv-1: Literature-Based Review, Kevin Fei Xia Mar 2016

Peptide Inhibitors That Disrupt Viral-Viral Protein Interactions In Hiv-1: Literature-Based Review, Kevin Fei Xia

Undergraduate Research Symposium

Protein-protein interactions play a key role during each stage of the viral life cycle. Thus, inhibiting viral protein-protein interactions with the peptides derived from the binding interfaces is a promising approach for potential multi-epitope based treatments. However, molecular mechanisms underlying these protein interactions are not yet fully understood. This study is focused on a comprehensive literature survey to identify known peptide inhibitors derived from the HIV-1 viral proteins, which disrupt viral-viral protein interactions. We also discuss the limitations of these studies in identifying the most promising candidates. The candidate domains/regions identified in this survey will be used in the ...